In the last two decades, thyroglobulin autoantibodies (TgAb) measurement has progressively switched from marker of thyroid autoimmunity to test associated with thyroglobulin (Tg) to verify the ...presence or absence of TgAb interference in the follow-up of patients with differentiated thyroid cancer. Of note, TgAb measurement is cumbersome: despite standardization against the International Reference Preparation MRC 65/93, several studies demonstrated high inter-method variability and wide variation in limits of detection and in reference intervals. Taking into account the above considerations, the main aim of the present study was the determination of TgAb upper reference limit (URL), according to the National Academy of Clinical Biochemistry guidelines, through the comparison of eleven commercial automated immunoassay platforms.
The sera of 120 healthy males, selected from a population survey in the province of Verona, Italy, were tested for TgAb concentration using eleven IMA applied on as many automated analyzers: AIA-2000 (AIA) and AIA-CL2400 (CL2), Tosoh Bioscience; Architect (ARC), Abbott Diagnostics; Advia Centaur XP (CEN) and Immulite 2000 XPi (IMM), Siemens Healthineers; Cobas 6000 (COB), Roche Diagnostics; Kryptor (KRY), Thermo Fisher Scientific BRAHMS, Liaison XL (LIA), Diasorin; Lumipulse G (LUM), Fujirebio; Maglumi 2000 Plus (MAG), Snibe and Phadia 250 (PHA), Phadia AB, Thermo Fisher Scientific. All assays were performed according to manufacturers' instructions in six different laboratories in Friuli-Venezia Giulia and Veneto regions of Italy Lab 1 (AIA), Lab 2 (CL2), Lab 3 (ARC, COB and LUM), Lab 4 (CEN, IMM, KRY and MAG), Lab 5 (LIA) and Lab 6 (PHA). Since TgAb values were not normally distributed, the experimental URL (e-URL) was established at 97.5 percentile according to the non-parametric method.
TgAb e-URLs showed a significant inter-method variability. Considering the same method, e-URL was much lower than that suggested by manufacturers (m-URL), except for ARC and MAG. Correlation and linear regression were unsatisfactory. Consequently, the agreement between methods was poor, with significant bias in Bland-Altman plot.
Despite the efforts for harmonization, TgAb methods cannot be used interchangeably. Therefore, additional effort is required to improve analytical performance taking into consideration approved protocols and guidelines. Moreover, TgAb URL should be used with caution in the management of differentiated thyroid carcinoma patients since the presence and/or the degree of TgAb interference in Tg measurement has not yet been well defined.
Shunt active power filters are devices, connected in parallel with nonlinear and reactive loads, which are in charge of compensating these characteristics in order to assure the quality of the ...distribution network. This paper analyzes the dynamics of a dc bus split-capacitor boost converter used as an active filter and proposes a control system which guarantees the desired closed-loop performance (unity power factor and load-current harmonics and reactive-power compensation). The proposed controller is hierarchically decomposed into two control loops, one in charge of shaping the network current and the other in charge of assuring the power balance. Unlike previous works that appeared in the literature, both control loops are analytically tuned. This paper describes the analytical design of the controller and presents some experimental results that show the good performance of the closed-loop system.
Oral immunization of healthy adults with 10⁷ CFU BCG Moreau Rio de Janeiro was well tolerated and significantly boosted gamma interferon responses to purified protein derivative, Ag85, and MPB70 from ...previous childhood intradermal BCG immunization. Oral BCG offers the possibility of a needle-free tuberculosis vaccine and of boosting the protective immunity from intradermal tuberculosis vaccines.
The most comprehensive and user-friendly photographic field guide to the world’s wildcats and hyenas
From the Leopard Cat of Asia, the Black-footed Cat of Africa, and the Amur Tiger of Siberia to ...South America’s Ocelots and North America’s Bobcats, the wildcats known as felids are among the most fascinating and spectacular of all animals. This stunningly illustrated book is the most comprehensive and user-friendly guide to the world’s felids and their often misunderstood relative, the hyenas. Covering and illustrating every species and subspecies, the guide features more than 150 superb full-color plates that incorporate more than 600 photographs and show species in similar poses for quick and easy comparison. Drawing on the latest taxonomy and research, the facing-page species accounts provide distribution maps, common and scientific names, and detailed information on key identification features, distribution, behavior, reproduction, similar species, habitat, conservation status, and where to observe each species. An ideal field companion for use anywhere in the world, the book will appeal to both casual nature enthusiasts and seasoned professionals.
Covers 41 felids and 4 hyenas—every species and subspecies in the world
Features more than 150 color plates incorporating more than 600 photos
Depicts species in similar poses for quick and easy comparisons
Provides key identification information in detailed, facing-page species accounts
Uses the latest taxonomy
Includes easy-to-read distribution maps and tips on where to observe each species
Many studies have established a role for oxidative stress and mitochondrial dysfunction as an important mechanism in the pathogenesis of neuronal disorders. Metalloporphyrins are a class of catalytic ...antioxidants that are capable of detoxifying a wide range of reactive oxygen species. The AEOL112 series of glyoxylate metalloporphyrins were designed with increased lipid solubility for better oral bioavailability and penetration of the blood-brain barrier. The goal of this study was to develop an in vitro assay using rat brain mitochondria to reliably detect endogenously released hydrogen peroxide (H(2)O(2)) and identify glyoxylate metalloporphyrins based on rank order of potency for removal of physiologically relevant H(2)O(2). A polarographic method was established for the sensitive, accurate, and reproducible detection of low levels of H(2)O(2). The assay identified several potent glyoxylate metalloporphyrins with H(2)O(2) scavenging potencies (IC(50)) in the nanomolar range. These results provide a simplified in vitro model system to detect physiologically generated mitochondrial H(2)O(2) as a screening tool to predict the biological efficacy of potential therapeutic entities.
The expected increase of hydrogen fuel cell vehicles has motivated the emergence of a significant number of studies on Hydrogen Refuelling Stations (HRS). Some of the main HRS topics are sizing, ...location, design optimization, and optimal operation. On-site green HRS, where hydrogen is produced locally from green renewable energy sources, have received special attention due to their contribution to decarbonization. This kind of HRS are complex systems whose hydraulic and electric linked topologies include renewable energy sources, electrolyzers, buffer hydrogen tanks, compressors and batteries, among other components. This paper develops a linear model of a real on-site green HRS that is set to be built in Zaragoza, Spain. This plant can produce hydrogen either from solar energy or from the utility grid and is designed for three different types of services: light-duty and heavy-duty fuel cell vehicles and gas containers. In the literature, there is a lack of online control solutions developed for HRS, even more in the form of optimal online control. Hence, for the HRS operation, a Model Predictive Controller (MPC) is designed to solve a weighted multi-objective online optimization problem taking into account the plant dynamics and constraints as well as the disturbances prediction. Performance is analysed throughout 210 individual month-long simulations and the effect of the multi-objective weighting, prediction horizon, and hydrogen selling price is discussed. With the simulation results, this work shows the suitability of MPC for HRS control and its significant economic advantage compared to the rule-based control solution. In all simulations, the MPC operation fulfils all required services. Moreover, results show that a seven-day prediction horizon can improve profits by 57% relative to a one-day prediction horizon; that the battery is under-sized; or that the MPC operation strategy is more resolutive for low hydrogen selling prices.
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•Model Predictive control application for green hydrogen refuelling station.•Linear modelling of green hydrogen refuelling station.•Parameter sensitivity analysis of the multi-objective function.•Prediction horizon sensitivity analysis.•Model Predictive Control performance advantage compared to rule-based control.
Canids of the world Castelló, José R
2018., 20180911, 2018, Letnik:
116
eBook
The most complete and user-friendly photographic field guide to the world’s canids This stunningly illustrated and easy-to-use field guide covers every species of the world’s canids, from the Gray ...Wolf of North America to the dholes of Asia, from African jackals to the South American Bush Dog. It features more than 150 superb color plates depicting every kind of canid and detailed facing-page species accounts that describe key identification features, morphology, distribution, subspeciation, habitat, and conservation status in the wild. The book also includes distribution maps and tips on where to observe each species, making Canids of the World the most comprehensive and user-friendly guide to these intriguing and spectacular mammals. Covers every species and subspecies of canid Features more than 150 color plates with more than 600 photos from around the globe Depicts species in similar poses for quick and easy comparisons Describes key identification features, habitat, behavior, reproduction, and much more Draws on the latest taxonomic research Includes distribution maps and tips on where to observe each species The ideal field companion and a delight for armchair naturalists
Introduction
Growth hormone (GH) consumption is the object of a particular attention by regulatory bodies, due to its financial impact; nevertheless, GH treatment has been demonstrated to be ...cost-effective and is, therefore, usually reimbursed by public health service systems. In Italy, significant differences in GH consumption between regions have been recorded. Different appropriateness in real practice is a possible explanation, but the proportion of drug wasted due to different combinations of therapeutic regimes and types of devices used in drug administration is a complementary explanation. Aim of the study is, therefore, to determine how much of the variability in GH consumption is actually due to differences in clinical practice, and how much to waste.
Materials and methods
A model was settled to estimate the population with indication for GH administration, separately for children, transition subjects and adults, based on both the scientific evidence available and directly collected clinical evaluations. A systematic literature search was conducted using Cochrane Library (HTA and NHSEE) databases, Medline via Ovid, Econlit via Ovid, Embase.
Conclusion
The model applied to the Italian population showed that there was apparently unexplainable over-prescription and potential under-prescription in various regions, ranging from 20 to 40 % less than the estimated theoretical consumption to over 200 %. Wastage, at level of single device, could amount to as much as 15 % of the consumption, demonstrating that price per mg is not in general a good proxy of the cost per mg of therapy. Our estimates of the wastage shows a significant potential gap in the model assessment of the HTA bodies, as far as they do not explicitly take into account the issue of wastage and, consequently, the actual variability in local clinical practice.
Bacille Calmette-Guerin (BCG) is currently the only available vaccine against tuberculosis (TB) and comprises a heterogeneous family of sub-strains with genotypic and phenotypic differences. The ...World Health Organization (WHO) affirms that the characterization of BCG sub-strains, both on genomic and proteomic levels, is crucial for a better comprehension of the vaccine. In addition, these studies can contribute in the development of a more efficient vaccine against TB. Here, we combine two-dimensional electrophoresis (2DE) and mass spectrometry to analyse the proteomic profile of culture filtrate proteins (CFPs) from M. bovis BCG Moreau, the Brazilian vaccine strain, comparing it to that of BCG Pasteur. CFPs are considered of great importance given their dominant immunogenicity and role in pathogenesis, being available for interaction with host cells since early infection.
The 2DE proteomic map of M. bovis BCG Moreau CFPs in the pH range 3-8 allowed the identification of 158 spots corresponding to 101 different proteins, identified by MS/MS. Comparison to BCG Pasteur highlights the great similarity between these BCG strains. However, quantitative analysis shows a higher expression of immunogenic proteins such as Rv1860 (BCG1896, Apa), Rv1926c (BCG1965c, Mpb63) and Rv1886c (BCG1923c, Ag85B) in BCG Moreau when compared to BCG Pasteur, while some heat shock proteins, such as Rv0440 (BCG0479, GroEL2) and Rv0350 (BCG0389, DnaK), show the opposite pattern.
Here we report the detailed 2DE profile of CFPs from M. bovis BCG Moreau and its comparison to BCG Pasteur, identifying differences that may provide relevant information on vaccine efficacy. These findings contribute to the detailed characterization of the Brazilian vaccine strain against TB, revealing aspects that may lead to a better understanding of the factors leading to BCG's variable protective efficacy against TB.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Tuberculosis (TB) is the second greatest killer worldwide that is caused by a single infectious agent. For its control, studies of TB vaccines are needed. Since Bacillus Calmette-Guerin (BCG) is the ...only vaccine against TB currently in use, studies addressing the protective role of BCG in the context of inducible inflammatory mediators are urgently required.
In this study, groups of HIV-negative adult healthy donors (HD; n = 42) and neonates (UV; n = 18) have been voluntarily enrolled, and BCG Moreau strain was used for the in vitro mononuclear cell infections for an initial period of 48 h. Subsequently, harvested conditioned medium (CM) was added to autologous resting cells for an additional 24, 48, and 120 h, and Annexin V, in conjunction with a vital dye, was then used for apoptosis detection. CM was also assayed for nitric oxide (NO), prostaglandin E2 (PGE2), leukotriene B4 (LTB4), interferon (IFN)-β, and transforming growth factor (TGF)-β1 levels. The p values were set up for any differences between two groups of individuals using Student's t-test and considered significant when ≤ 0.05.
At 120 h, CM induced the highest apoptosis levels in both group studied, but necrosis was high in UV group only (p-value < 0.05). NO was released equally during BCG infection in both groups, but higher levels were found in HD when compared with UV group (p-value < 0.05). Overall, BCG Moreau triggered high PGE2, LTB4 and IFN-β productions in macrophages from the UV group (p-value ≤ 0.05), whereas the prostanoid PGE2 and TGF-β1 had an opposite pattern in the HD group.
This study uncovers critical roles for endogenous compounds in the instruction of host macrophage cell death patterns. Understanding the regulation of human immune responses is critical for vaccine development and the treatment of infectious diseases. These findings shed new light on the potential condition for a booster immunization in individuals already vaccinated with BCG for TB protection, and further studies are warranted.
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