The aim was to determine the changes of inflammatory mediator expression in human macrophages stimulated with outer membrane vesicles purified from Porphyromonas gingivalis.
outer membrane vesicles ...purified by ultracentrifugation from ATCC 33277 and W83 P. gingivalis strains were used for stimulating human macrophages and determine their inflammatory mediator expression changes. U937 monocyte cells line were differentiated into macrophages and stimulated with outer membrane vesicles for 30 min and six hours. In Independent experiments, the outer membrane vesicles and viable bacteria control were pre-treated with the gingipain inhibitors KYT-1 and KYT-36 (Arg-gingipain and Lys-gingipain, respectively) or Polymyxin-B to block the lipopolysaccharide activity to evaluate the secretion changes of immune mediators IL-1β, IL-6, TNF-α, IL-8, MCP-1, MIP-1α and RANTES by flow cytometry. A factorial ANOVA was used to analyze the data.
The outer membrane vesicles of P. gingivalis ATCC 33277 displayed higher Arg-gingipain activity than those obtained from the P. gingivalis W83 strain (0.6 U/μg vs. 0.46 U/μg). Although the outer membrane vesicles of P. gingivalis stimulated the production of cytokines and chemokines, specific Arg-gingipain and Lys-gingipain inhibition induced significant increases in IL-1β, IL-6, IL-8, MCP-1, and RANTES levels, and this induction was significantly greater at 6 h compared to 30 min (*p < 0.05). On the contrary, TNF-α secretion decreased when gingipains were blocked.
outer membrane vesicles may play a dual role during P. gingivalis infection based on their ability to induce changes in the immune responses of human macrophages, probably via gingipain-dependent events.
•Outer membrane vesicles P.gingivalis have important Arg-gingipain activity.•Outer membrane vesicles P.gingivalis modulate the macrophage immune response.•Outer membrane vesicles P.gingivalis induce transcriptional changes of cytokines.
We report on the production of inclusive Υ (1S) and Υ (2S) in p–Pb collisions at √ s NN = 5.02 TeV at the LHC. The measurement is performed with the ALICE detector at backward (−4.46 < y cms < −2.96) ...and forward (2.03 < y cms < 3.53) rapidity down to zero transverse momentum. The production cross sections of the Υ (1S) and Υ (2S) are presented, as well as the nuclear modification factor and the ratio of the forward to backward yields of Υ (1S). A suppression of the inclusive Υ (1S) yield in p–Pb collisions with respect to the yield from pp collisions scaled by the number of binary nucleon–nucleon collisions is observed at forward rapidity but not at backward rapidity. The results are compared to theoretical model calculations including nuclear shadowing or partonic energy loss effects. (creativecommons.org/licenses/by/3.0/). Funded by SCOAP 3 .
Two-particle angular correlations between unidentified charged trigger and associated particles are measured by the ALICE detector in p–Pb collisions at a nucleon–nucleon centre-of-mass energy of ...5.02 TeV. The transverse-momentum range 0.7 < p T,assoc < p T,trig < 5.0 GeV/c is examined, to include correlations induced by jets originating from low momentum-transfer scatterings (minijets). The correlations expressed as associated yield per trigger particle are obtained in the pseudorapidity range |η| < 0.9. The near-side long-range pseudorapidity correlations observed in high-multiplicity p–Pb collisions are subtracted from both near-side short-range and away-side correlations in order to remove the non-jet-like components. The yields in the jet-like peaks are found to be invariant with event multiplicity with the exception of events with low multiplicity. This invariance is consistent with the particles being produced via the incoherent fragmentation of multiple parton–parton scatterings, while the yield related to the previously observed ridge structures is not jet-related. The number of uncorrelated sources of particle production is found to increase linearly with multiplicity, suggesting no saturation of the number of multi-parton interactions even in the highest multiplicity p–Pb collisions. Further, the number scales only in the intermediate multiplicity region with the number of binary nucleon–nucleon collisions estimated with a Glauber Monte-Carlo simulation. (creativecommons.org/licenses/by/3.0/). Funded by SCOAP 3 .
The measurement of two-particle angular correlations is a powerful tool to study jet quenching in a $p_{\mathrm{T}}$ region inaccessible by direct jet identification. In these measurements ...pseudorapidity ($\Delta\eta$) and azimuthal ($\Delta\varphi$) differences are used to extract the shape of the near-side peak formed by particles associated to a higher $p_{\mathrm{T}}$ trigger particle ($1 < p_{\mathrm{T, trig}} <$ 8 GeV/c). A combined fit of the near-side peak and long-range correlations is applied to the data allowing the extraction of the centrality evolution of the peak shape in Pb-Pb collisions at $\sqrt{s_{\mathrm{NN}}}$ = 2.76 TeV. A significant broadening of the peak in the $\Delta\eta$ direction at low $p_{\mathrm{T}}$ is found from peripheral to central collisions, which vanishes above 4 GeV/c, while in the $\Delta\varphi$ direction the peak is almost independent of centrality. For the 10% most central collisions and $1 < p_{\mathrm{T, assoc}} <$ 2 GeV/c, $1 < p_{\mathrm{T, trig}} <$ 3 GeV/c a novel feature is observed: a depletion develops around the centre of the peak. The results are compared to pp collisions at the same centre of mass energy and to AMPT model simulations. The comparison to the investigated models suggests that the broadening and the development of the depletion is connected to the strength of radial and longitudinal flow.
The production of muons from heavy-flavour hadron decays in p-Pb collisions at $\sqrt{{\textit s}_{\rm NN}}=5.02$ TeV was studied for $2 < p_{\rm T} < 16$ GeV/$c$ with the ALICE detector at the CERN ...LHC. The measurement was performed at forward (p-going direction) and backward (Pb-going direction) rapidity, in the ranges of rapidity in the center-of-mass system (cms) $2.03<y_{\rm cms}<3.53$ and $-4.46<y_{\rm cms}<-2.96$, respectively. The production cross sections and nuclear modification factors are presented as a function of transverse momentum ($p_{\rm T}$). At forward rapidity, the nuclear modification factor is compatible with unity while at backward rapidity, in the interval $2.5<p_{\rm T}<3.5$ GeV/$c$, it is above unity by more than 2$\sigma$. The ratio of the forward-to-backward production cross sections is also measured in the overlapping interval $2.96 < \vert y_{\rm cms} \vert < 3.53$ and is smaller than unity by 3.7$\sigma$ in $2.5<p_{\rm T}<3.5$ GeV/$c$. The data are described by model calculations including cold nuclear matter effects.
In two-particle angular correlation measurements, jets give rise to a near-side peak, formed by particles associated to a higher $p_{\mathrm{T}}$ trigger particle. Measurements of these correlations ...as a function of pseudorapidity ($\Delta\eta$) and azimuthal ($\Delta\varphi$) differences are used to extract the centrality and $p_{\mathrm{T}}$ dependence of the shape of the near-side peak in the $p_{\mathrm{T}}$ range $1 < p_{\mathrm{T}} <$ 8 GeV/c in Pb-Pb and pp collisions at $\sqrt{s_{\mathrm{NN}}}$ = 2.76 TeV. A combined fit of the near-side peak and long-range correlations is applied to the data and the peak shape is quantified by the variance of the distributions. While the width of the peak in the $\Delta\varphi$ direction is almost independent of centrality, a significant broadening in the $\Delta\eta$ direction is found from peripheral to central collisions. This feature is prominent for the low $p_{\mathrm{T}}$ region and vanishes above 4 GeV/c. The widths measured in peripheral collisions are equal to those in pp in the $\Delta\varphi$ direction and above 3 GeV/c in the $\Delta\eta$ direction. Furthermore, for the 10\% most central collisions and $1 < p_{\mathrm{T, assoc}} <$ 2 GeV/c, $1 < p_{\mathrm{T, trig}} <$ 3 GeV/c a departure from a Gaussian shape is found: a depletion develops around the centre of the peak. The results are compared to AMPT model simulations as well as other theoretical calculations indicating that the broadening and the development of the depletion is connected to the strength of radial and longitudinal flow.
The inclusive J/$\psi$ production has been studied in Pn-Pb and pp collisions at the centre-of-mass energy per nucleon pair $\sqrt{s_{\rm NN}}=5.02$ TeV, using the ALICE detector at the CERN LHC. The ...J/$\psi$ meson is reconstructed, in the centre-of-mass rapidity interval $2.5<y<4$ and in the transverse-momentum range $p_{\rm T}<12$ GeV/$c$, via its decay to a muon pair. In this Letter, we present results on the inclusive J/$\psi$ cross section in pp collisions at $\sqrt{s}=5.02$ TeV and on the nuclear modification factor $R_{\rm AA}$. The latter is presented as a function of the centrality of the collision and, for central collisions, as a function of the transverse momentum $p_{\rm T}$ of the J/$\psi$. The measured $R_{\rm AA}$ values indicate a suppression of the J/$\psi$ in nuclear collisions and are then compared to our previous results obtained in Pb-Pb collisions at $\sqrt{s_{\rm NN}}=2.76$ TeV. The ratio of the $R_{\rm AA}$ values at the two energies is also computed and compared to calculations of statistical and dynamical models. The numerical value of the ratio for central events (0-10\% centrality) is $1.17 \pm 0.04 {\rm{(stat)}}\pm 0.20 {\rm{(syst)}}$. In central events, as a function of $p_{\rm T}$, a slight increase of $R_{\rm AA}$ with collision energy is visible in the region $2<p_{\rm T}<6$ GeV/$c$. Theoretical calculations provide a good description of the measurements, within uncertainties.
The production of beauty hadrons was measured via semi-leptonic decays at mid-rapidity with the ALICE detector at the LHC in the transverse momentum interval $1<p_{\rm T}<8$ GeV/$c$ in minimum-bias ...p-Pb collisions at $\sqrt{s_{\rm NN}}=5.02$ TeV and in $1.3<p_{\rm T}<8$ GeV/$c$ in the 20% most central Pb-Pb collisions at $\sqrt{s_{\rm NN}}=2.76$ TeV. The pp reference spectra at $\sqrt{s}=5.02$ TeV and $\sqrt{s}=2.76$ TeV, needed for the calculation of the nuclear modification factors $R_{\rm pPb}$ and $R_{\rm PbPb}$, were obtained by a pQCD-driven scaling of the cross section of electrons from beauty-hadron decays measured at $\sqrt{s}=7$ TeV. The $R_{\rm PbPb}$ is about 0.7 with an uncertainty of about 30% in the interval $3<p_{\rm T}<6$ GeV/$c$ and 0.47 with an uncertainty of 25% in $6<p_{\rm T}<8$ GeV/$c$ in Pb-Pb collisions. Below $p_{\rm T}=3$ GeV/$c$, the $R_{\rm PbPb}$ values increase with decreasing transverse momentum with systematic uncertainties of 30-45%. The $R_{\rm pPb}$ is consistent with unity within systematic uncertainties of about 20% at high $p_{\rm T}$, increasing at low $p_{\rm T}$, and is well described by theoretical calculations that include cold nuclear matter effects in p-Pb collisions. The measured $R_{\rm pPb}$ and these calculations indicate that cold nuclear matter effects are small at high transverse momentum also in Pb-Pb collisions. Therefore, the observed reduction of $R_{\rm PbPb}$ below unity for high $p_{\rm T}$ can be ascribed to an effect of the hot and dense medium formed in Pb-Pb collisions.
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