Summary
The Orchidaceae is a diverse and ecologically important plant family. Approximately 69% of all orchid species are epiphytes, which provide diverse microhabitats for many small animals and ...fungi in the canopy of tropical rainforests. Moreover, many orchids are of economic importance as food flavourings or ornamental plants. Phalaenopsis aphrodite, an epiphytic orchid, is a major breeding parent of many commercial orchid hybrids. We provide a high‐quality chromosome‐scale assembly of the P. aphrodite genome. The total length of all scaffolds is 1025.1 Mb, with N50 scaffold size of 19.7 Mb. A total of 28 902 protein‐coding genes were identified. We constructed an orchid genetic linkage map, and then anchored and ordered the genomic scaffolds along the linkage groups. We also established a high‐resolution pachytene karyotype of P. aphrodite and completed the assignment of linkage groups to the 19 chromosomes using fluorescence in situ hybridization. We identified an expansion in the epiphytic orchid lineage of FRS5‐like subclade associated with adaptations to the life in the canopy. Phylogenetic analysis further provides new insights into the orchid lineage‐specific duplications of MADS‐box genes, which might have contributed to the variation in labellum and pollinium morphology and its accessory structure. To our knowledge, this is the first orchid genome to be integrated with a SNP‐based genetic linkage map and validated by physical mapping. The genome and genetic map not only offer unprecedented resources for increasing breeding efficiency in horticultural orchids but also provide an important foundation for future studies in adaptation genomics of epiphytes.
Abstract
The industrialization of quantum dot light-emitting diodes (QLEDs) requires the use of less hazardous cadmium-free quantum dots, among which ZnSe-based blue and InP-based green and red ...quantum dots have received considerable attention. In comparison, the development of InP-based green QLEDs is lagging behind. Here, we prepare green InP/ZnSe/ZnS quantum dots with a diameter of 8.6 nm. We then modify the InP quantum dot emitting layer by passivation with various alkyl diamines and zinc halides, which decreases electron mobility and enhances hole transport. This, together with optimizing the electron transport layer, leads to green 545 nm InP QLEDs with a maximum quantum efficiency (EQE) of 16.3% and a current efficiency 57.5 cd/A. EQE approaches the theoretical limit of InP quantum dots, with an emission quantum yield of 86%.
Nanoscale carbon materials hold great promise for biotechnological and biomedical applications. Fluorescent nanodiamond (FND) is a recent new addition to members of the nanocarbon family. Here, we ...report long-term in vivo imaging of FNDs in Caenorhabditis elegans (C. elegans) and explore the nano-biointeractions between this novel nanomaterial and the model organism. FNDs are introduced into wild-type C. elegans by either feeding them with colloidal FND solution or microinjecting FND suspension into the gonads of the worms. On feeding, bare FNDs stay in the intestinal lumen, while FNDs conjugated with biomolecules (such as dextran and bovine serum albumin) are absorbed into the intestinal cells. On microinjection, FNDs are dispersed in the gonad and delivered to the embryos and eventually into the hatched larvae in the next generation. The toxicity assessments, performed by employing longevity and reproductive potential as physiological indicators and measuring stress responses with use of reporter genes, show that FNDs are stable and nontoxic and do not cause any detectable stress to the worms. The high brightness, excellent photostability, and nontoxic nature of the nanomaterial have enabled continuous imaging of the whole digestive system and tracking of the cellular and developmental processes of the living organism for several days.
Frailty is associated with major health outcomes. However, the relationships between frailty and frailty symptoms haven't been well studied. This study aims to show the associations between frailty ...and frailty symptoms. The Health and Retirement Study (HRS) is an ongoing longitudinal biannual survey in the United States. Three of the most used frailty diagnoses, defined by the Functional Domains Model, the Burden Model, and the Biologic Syndrome Model, were reproduced according to previous studies. The associations between frailty statuses and input symptoms were assessed using odds ratios and correlation coefficients. The sample sizes, mean ages, and frailty prevalence matched those reported in previous studies. Frailty statuses were weakly correlated with each other (coefficients = 0.19 to 0.38, p 0.05 for all). One to six symptoms defined by the other two models were not significantly correlated with each of the three frailty statuses (p > 0.05 for all). Frailty statuses were significantly correlated with their own bias variables (p < 0.05 for all). Frailty diagnoses lack significant correlations with some of their own frailty symptoms and some of the frailty symptoms defined by the other two models. This finding raises questions like whether the frailty symptoms lacking significant correlations with frailty statuses could be included to diagnose frailty and whether frailty exists and causes frailty symptoms.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
It has been established that the transfer of human adaptive impedance is of great significance for physical human-robot interaction (pHRI). By processing the electromyography (EMG) signals collected ...from human muscles, the limb impedance could be extracted and transferred to robots. The existing impedance transfer interfaces rely only on visual feedback and, thus, may be insufficient for skill transfer in a sophisticated environment. In this paper, physical haptic feedback mechanism is introduced to result in muscle activity that would generate EMG signals in a natural manner, in order to achieve intuitive human impedance transfer through a designed coupling interface. Relevant processing methods are integrated into the system, including the spectral collaborative representation-based classifications method used for hand motion recognition; fast smooth envelop and dimensionality reduction algorithm for arm endpoint stiffness estimation. The tutor's arm endpoint motion trajectory is directly transferred to the robot by the designed coupling module without the restriction of hands. Haptic feedback is provided to the human tutor according to skill learning performance to enhance the teaching experience. The interface has been experimentally tested by a plugging-in task and a cutting task. Compared with the existing interfaces, the developed one has shown a better performance. Note to Practitioners -This paper is motivated by the limited performance of skill transfer in the existing human-robot interfaces. Conventional robots perform tasks independently without interaction with humans. However, the new generation of robots with the characteristics, such as flexibility and compliance, become more involved in interacting with humans. Thus, advanced human robot interfaces are required to enable robots to learn human manipulation skills. In this paper, we propose a novel interface for human impedance adaptive skill transfer in a natural and intuitive manner. The developed interface has the following functionalities: 1) it transfers human arm impedance adaptive motion to the robot intuitively; 2) it senses human motion signals that are decoded into human hand gesture and arm endpoint stiffness that ia employed for natural human robot interaction; and 3) it provides human tutor haptic feedback for enhanced teaching experience. The interface can be potentially used in pHRI, teleoperation, human motor training systems, etc.
Zeolitic Imidazolate Framework-8 (ZIF-8), for the first time for ZIFs, exhibits a remarkable capacity for the anticancer drug 5-fluorouracil (5-FU), around 660 mg of 5-FU/g of ZIF-8, and presents a ...pH-triggered controlled drug release property. These prove ZIF-8 to be a valuable candidate for delivery of anticancer agents and reveal its potential applications in the treatment of cancer.
Metal–organic polyhedra (MOPs) are promising candidates for many potential applications; however, their use as photocatalysts for hydrogen production has yet to be developed. Herein, the ...photocatalytic performance of a water‐stable Zr‐MOP, ZrT‐1‐NH2, was evaluated, for the first time, through photocatalytic hydrogen evolution under visible‐light irradiation. ZrT‐1‐NH2 shows clearly enhanced photocatalytic activity (510.42 μmol g−1 h−1) for hydrogen production, in comparison with that of other homogeneous crystalline materials. If platinum nanoparticles were introduced into the photocatalytic system, the hydrogen production efficiency of ZrT‐1‐NH2 could be further improved. For ZrT‐1‐NH2, the conspicuous improvement in photocatalysis can be attributed to efficient electron–hole separation, targeted electron transfer, and excellent recombination suppression. Furthermore, ZrT‐1‐NH2 shows excellent stability during photocatalytic hydrogen evolution over five continuous runs. This work illustrates that MOP‐based photocatalysts hold promise for broad applications in the domain of clean energy.
MOPping up light: Metal–organic polyhedra (MOPs) are promising candidates for many potential applications, but have not previously been reported to act as photocatalysts for hydrogen production. For the first time, a molecular tetrahedron, as a representative zirconium‐based MOP, is demonstrated to act as a photocatalyst with fairly high catalytic activity for hydrogen production (see figure).
Electrolyte concentration is crucial for low-temperature aqueous batteries (LTABs) as it directly dictates electrolyte freezing point. However, the conventional approach for identifying suitable ...concentrations relies on determining freezing points of a large number of concentration combinations in the given H 2 O–solute system, which is inefficient, particularly for multiple-solute systems. Here, we propose an approach to efficiently and rationally design anti-freezing electrolyte concentrations via the freeze concentration process. Freeze concentration is a process of concentrating dilute solution by precipitating ice or hydrates at target low temperatures ( T t ). For single-solute systems, the frozen concentrated electrolyte (FCE) extracted from the liquid–ice mixture has, by nature, the lowest concentration (therefore the lowest cost) that remains unfrozen at T t . For multiple-solute systems, instead of testing a large number of concentration combinations, the proposed approach can directly determine suitable concentrations via one freeze concentration experiment at T t . As a demonstration, we successfully designed FCEs in H 2 O–LiCl, H 2 O–NaClO 4 , and H 2 O–NaClO 4 –NaCF 3 COO systems, and demonstrated superior performance in Li-based LiMn 2 O 4 //3,4,9,10-perylenetetracarboxylic diimide (PTCDI) and Na-based Na 1.4 CoFe(CN) 6 0.84 ·2.5H 2 O//PTCDI full cells. This work provides a universal and efficient strategy to design electrolyte concentrations for LTABs.
One great challenge in understanding the history of life is resolving the influence of environmental change on biodiversity. Simulated annealing and genetic algorithms were used to synthesize data ...from 11,000 marine fossil species, collected from more than 3000 stratigraphic sections, to generate a new Cambrian to Triassic biodiversity curve with an imputed temporal resolution of 26 ± 14.9 thousand years. This increased resolution clarifies the timing of known diversification and extinction events. Comparative analysis suggests that partial pressure of carbon dioxide (
co
) is the only environmental factor that seems to display a secular pattern similar to that of biodiversity, but this similarity was not confirmed when autocorrelation within that time series was analyzed by detrending. These results demonstrate that fossil data can provide the temporal and taxonomic resolutions necessary to test (paleo)biological hypotheses at a level of detail approaching those of long-term ecological analyses.
Clinical evidence suggests that conventional cardiovascular disease (CVD) risk factors cannot explain all CVD incidences. Recent studies have shown that telomere attrition, clonal hematopoiesis of ...indeterminate potential (CHIP), and atherosclerosis (telomere–CHIP–atherosclerosis, TCA) evolve to play a crucial role in CVD. Telomere dynamics and telomerase have an important relationship with age-related CVD. Telomere attrition is associated with CHIP. CHIP is commonly observed in elderly patients. It is characterized by an increase in blood cell clones with somatic mutations, resulting in an increased risk of hematological cancer and atherosclerotic CVD. The most common gene mutations are DNA methyltransferase 3 alpha (DNMT3A), Tet methylcytosine dioxygenase 2 (TET2), and additional sex combs-like 1 (ASXL1). Telomeres, CHIP, and atherosclerosis increase chronic inflammation and proinflammatory cytokine expression. Currently, their epidemiology and detailed mechanisms related to the TCA axis remain incompletely understood. In this article, we reviewed recent research results regarding the development of telomeres and CHIP and their relationship with atherosclerotic CVD.
PDF
