Hydraulic systems in production equipment are relatively complex, involving various time series sensor data, such as operating sound frequencies and hydraulic pressure. However, fault prediction ...generally faces the bottleneck of insufficient fault data, leading to a severe imbalance between normal and fault data ratios. This imbalance makes it difficult for prediction models to effectively learn fault trends. In this paper, we propose a synthesis method for time series data to address this issue. The experimental results demonstrate that the synthesized data lead to significant improvements.
The sequential deletion method is commonly applied to locate the functional domain of a protein. Unfortunately, manually designing primers for multiplex polymerase chain reaction (PCR) is a ...labor-intensive task. In order to speed up the experimental procedure and to improve the efficiency of producing PCR products, this paper proposes a multiplex PCR primers (MPCRPs) designer to design multiple forward primers with a single 3′-UTR reverse primer for extracting various N-terminal truncated mutants to quickly locate the functional domain of a cDNA sequence. Several factors, including melting temperature, primer length, GC content, internal self-complement, cross-dimerization, terminal limitation, and specificity, are used as the criteria for designing primers. This study obtains a near-optimal solution of primer sets that can be placed in as few test tubes as possible for one multiplex PCR experiment.
Homo sapiens ribosomal protein L5, Homo sapiens xylosyltransferase I, and Bacteriophage T4 gene product 11 were used as test examples to verify efficacy of the proposed algorithm. In addition, the designed primers of Homo sapiens ribosomal protein L5 cDNA were applied in multiplex PCR experiments. A total of 48 forward primers and one reverse primer were designed and used to duplicate N-terminal truncated mutants of different lengths from the protein. The primers were classified into eight tube groups (i.e., test tubes) held within the same temperature range (53–57
°C), and the validity of the PCR products were verified using polyacrylamide gel electrophoresis (PAGE) with the functional domain correctly located. A software implementation of the proposed algorithm useful in assisting the researcher to design primers for multiplex PCR experiments was developed and available upon request.
Based on a compressed linear quadtree, a data structure is proposed which is more than a structure to compress a gray-level or color image, but a structure, which can be applied to retrieve a block ...image. This compressed linear quadtree may directly extract a detailed block image without decompressing the compressed data of the original image.
A minute lossy method for 2D-gel images compression Chan, Yung-Kuan; Liau, Duan-Li; Chen, Yung-Fu ...
International journal of imaging systems and technology,
2006, 2006-01-00, Letnik:
16, Številka:
1
Journal Article
Neutralizing antibodies (nAbs) are a critical component for protection against dengue virus (DENV) infection, but little is known about the immune mechanisms governing their induction and whether ...such mechanisms can be harnessed for vaccine development. In this study, we profiled the early immune responses to flaviviruses in human peripheral blood mononuclear cells and screened a panel of toll-like receptor (TLR) agonists that stimulate the same immune signatures. Monocyte/macrophage-driven inflammatory responses and interferon responses were characteristics of flavivirus infection and associated with induction of nAbs in humans immunized with the yellow fever vaccine YF-17D. The signatures were best reproduced by the combination of TLR agonists Pam
CSK
and PolyI:C (PP). Immunization of both mice and macaques with a poorly immunogenic recombinant DENV-2 envelope domain III (EDIII) induced more consistent nAb and CD4
T-cell responses with PP compared to alum plus monophosphoryl lipid A. Induction of nAbs by PP required interferon-mediated signals in macrophages in mice. However, EDIII + PP vaccination only provided partial protection against viral challenge. These results provide insights into mechanisms underlying nAb induction and a basis for further improving antigen/adjuvant combinations for dengue vaccine development.
This paper presents two image features, multi-orientation color complexity (MOCC) and color-space relation (CSR). MOCC refers to the color complexity. CSR concerns the spatial relations of similar ...color pixels in an image. By combining both features, an image retrieval system was developed. The experimental results revealed that such a system can perform expressively at accurate recognition rate. To further speed up this system, a clustering based filer was applied to quickly sieve out the most unqualified database images.
A Modified K-Means Algorithm - Two-Layer K-Means Algorithm Chen Chung Liu; Shao Wei Chu; Yung Kuan Chan ...
2014 Tenth International Conference on Intelligent Information Hiding and Multimedia Signal Processing,
2014-Aug.
Conference Proceeding
In this paper, a modified K-means algorithm is proposed to categorize a set of data. K-means algorithm is a simple and easy clustering method which can efficiently classify a large number of ...continuous numerical data of high-dimensions. Moreover, the data in each cluster are similar to one another. However, it is vulnerable to outliers and noisy data, and it spends much executive time in classifying data too. Noisy data, outliers, and the data with quite different values in one cluster may reduce the accuracy rate of data matching obtained by a pattern matching system since the cluster center cannot precisely describe the data in the cluster. Hence, this study provides a two-layer K-means algorithm to solve above problems. In experiment, several well-known data sets are used to evaluate the performance of proposed algorithm, and the two-layer K-means algorithm can give expressive experimental results.
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