An electric double layer (EDL) generally exists at the interface between a conductive electrode and its adjacent liquid electrolyte. Accurate measurement of the capacitance of EDL is requisite but a ...great challenge due to the complexity of its variation mechanism correlated with the magnitude and frequency of applied signals and the difficulty in measuring the inner layer potentials across the EDL. Herein, a novel dielectrophoresis (DEP)-based approach is proposed to measure the capacitance of an EDL at a microelectrode/electrolyte interface. The measurement is achieved by employing DEP manipulation to micro polystyrene (PS) spheres suspended in a liquid electrolyte and determining the capacitance of EDL on the microelectrodes from the moving velocities of spheres. This method allows measurement of the capacitances of EDL under alternating current (AC) signals with different magnitudes and frequencies, so that the capacitance change with the magnitude and frequency of the applied signal can be characterized. The method avoids the impedance interference from the liquid electrolyte, external measuring systems, and other crosstalks, enabling an accurate measurement of double layer capacitance. In addition, the inner layer potentials across EDL under different magnitudes and frequencies of applied signals are comprehensively investigated, which facilitates an understanding of the ion behavior at the interfacial boundary that governs external observations of electrochemical reactions. The accurate measurement of the capacitance of EDL is of significance to explore the mechanism of interfacial functioning of electrochemical and bioelectrical devices and systems.
Different decaying characteristics for two types of El Nino and their relationships with the summer rainfall in China were investigated by using 743 stations daily precipitation data from China ...Meteorological Administration,NCEP circulation reanalysis datasets and the monthly mean Met Office Hadley Center sea surface temperature(SST)analyses data.Based on the evolution of the SST anomalies (SSTA)across the equator(5°S-5°N),two types of decaying patterns are identified for EP-El Nino:(1)An evolution pattern that SSTA decaying from east to west(E-W).The positive SSTA larger than 0.52°C first disappear in offshore of South America and the disappearance extends to the west.(2)An evolution pattern that SSTA decaying from west to east(W-E).The positive SSTA larger than 0.5°Cfirst disappear in the central Pacific and the disappearance extends to the east.The decaying evolution of CP-El Nino is classified into three patterns:(1)A symmetric(S)—decaying pattern whose SSTA grows and decays symmetrically with respect to
The June‐July Yangtze flooding in 1998 and 2020 drew incredible attention owing to the extreme precipitation events and devastating societal/economic damages. However, the quantitative estimation of ...the moisture transport mechanism is intensely discussed but still unresolved. Here we investigated two events from a unique perspective of Eulerian atmospheric water tracers that tries to explain the two events from model physics. The results showed that the moisture supplies from the Northwest Pacific decreased despite of different inducements, whereas the southwest summer monsoon (SWSM)‐related moisture supplies exhibited conspicuous enhancements in both events, suggesting that the SWSM‐related moisture supplies controlled the occurrence of Yangtze flooding. The physical processes of the two events were further compared. The 2020 flooding was more severe than the 1998 event, which was related to the stronger advective convergences and in‐stratus condensations of the SWSM‐related moisture.
Plain Language Summary
In 1998 and 2020, the Yangtze River valley experienced extreme rainfalls during June–July. The associated atmospheric river was detected to be significantly enhanced by the moisture from the southwest summer monsoon‐related source regions (such as the Bay of Bengal, Arabian Sea, Tropical Indian Ocean, Indo‐China Peninsula, and India) in both events. On the contrary, the moisture supplies from the Northwest Pacific declined. These results suggested that the southwest summer monsoon‐related moisture supplies played vital roles in the formations of extreme rainfalls over the Yangtze River valley. Additionally, our study investigated the differences in the physical mechanisms of the two extreme rainfall events. The stronger advective transport and cloud processes forced more southwest summer monsoon‐related moisture to be converted into precipitation around the Yangtze River, ultimately leading to more severe flooding in 2020 than in 1998.
Key Points
The southwest summer monsoon (SWSM)‐related moisture supplies regulate the emergence of the Yangtze flooding during June–July
Along with the flooding appearances, the moisture supplies from the Northwest Pacific to the Yangtze River valley decrease
Stronger advective transport and in‐stratus condensations of the SWSM‐related moisture avail the larger flooding in 2020 than in 1998
Lung adenocarcinoma (LUAD) is the predominant type of non-small lung cancer (NSCLC) with strong invasive ability and poor prognosis. The drug resistance related genes are potentially associated with ...prognosis of LUAD. Our research aimed to identify the drug resistance related genes and explore their potential prognostic value in LUAD patients. The data used in this study were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Firstly, we screened drug resistance related genes in LUAD by differential gene analysis, univariate Cox regression and drug sensitivity analyses. Subsequently, we constructed a risk score model using LASSO Cox regression analysis, and verified whether the risk score can predict the survival of LUAD patients independent of other factors. Moreover, we explored the immune infiltration of 22 immune cells between high-risk and low-risk patients. Totally 10 drug-resistance positively related genes (PLEK2, TFAP2A, KIF20A, S100P, GDF15, HSPB8, SASH1, WASF3, LAMA3 and TCN1) were identified in LUAD. The risk score model of LUAD constructed with these 10 genes could reliably predict the prognosis of LUAD patients. 18 pathways were significantly activated in high-risk group compared with low-risk group. In addition, the infiltration proportion of multiple immune cells was significantly different between high-risk and low-risk groups, and the proportion of M1 phagocytes was significantly higher in the high-risk group compared with the low-risk group. The drug resistance related genes (PLEK2, TFAP2A, KIF20A, S100P, GDF15, HSPB8, SASH1, WASF3, LAMA3 and TCN1) could predict the prognosis of LUAD patients. Clarifying the roles and mechanisms of these 10 genes in regulating drug resistance in LUAD will help to improve individualized clinical treatment protocols and predict patient sensitivity to treatment.
To detect circulating tumor cells (CTCs) in the peripheral blood of patients with tumor, and to analyze the significance of CTC detection in tumor diagnosis and monitoring. In the present study, ...peripheral blood was collected from 125 patients with tumor, and CTCs were isolated and identified. Differences in CTC number and subtype detection were analyzed for different tumor diseases and stages. CTCs were detected in 122 of the 125 patients with tumor, with a positive rate of 97.6%. The number of CTCs increases in patients with vascular metastasis. The number of mesenchymal CTCs increases in patients with lymph node or vascular metastasis. The average ratio of epithelial CTCs in each positive sample decreases in the later stages of cancer compared with the earlier stages, while the average ratio of mesenchymal CTCs increases in the later stages of cancer compared with the earlier stages. The results showed that CTCs with mesenchymal phenotypes are closely related to lymph node or vascular metastasis. CTC detection can help with early diagnosis of tumor diseases. Continuous monitoring of changes in CTCs number and subtypes can assist clinical judgment of tumor disease development status and prognosis.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
In rice (Oryza sativa), amylose content (AC) is the major factor that determines eating and cooking quality (ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy ...(Wx) locus. Here we identified a rare Wx allele, Wxmw, which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wxmw may have derived from recombination between two important natural Wx alleles, Wxin and Wxb. We validated the effects of Wxmw on rice grain quality using both transgenic lines and near‐isogenic lines (NILs). When introgressed into the japonica Nipponbare (NIP) background, Wxmw resulted in a moderate AC that was intermediate between that of NILs carrying the Wxb allele and NILs with the Wxmp allele. Notably, mature grains of NILs fixed for Wxmw had an improved transparent endosperm relative to soft rice. Further, we introduced Wxmw into a high‐yielding japonica cultivar via molecular marker‐assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wxmw is a promising allele to improve grain quality, especially ECQ and grain transparency of high‐yielding japonica cultivars, in rice breeding programs.
In the present study, a rare Wx allele, Wxmw which may have derived from recombination between two important natural Wx alleles in rice, was identified as a promising allele to improve grain quality, especially cooked rice taste and grain transparency of high‐yielding japonica cultivars, in rice breeding programs.
Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death in the world, and has a relatively low survival rate. Long non-coding RNAs (lncRNAs) have been demonstrated to modulate ...cancer progression through a variety of molecular mechanisms. We sought to investigate the role and potential mechanism of MYC-induced long non-coding RNA (MINCR) in NSCLC.
Expression levels of MINCR was first identified using The Cancer Genome Atlas (TCGA), further confirmed with specimens from 29 NSCLC patients and three cell lines using qRT-PCR. Overexpression and knockdown of MINCR were performed in NSCLC cell lines through MINCR overexpression vectors and synthesized siRNAs, respectively. The roles of MINCR in NSCLC cell lines, such as cell proliferation, cell cycle arrest, and apoptosis, were identified by MTT, flow cytometry, and Western blot. The modulation of MINCR-regulated genes, including c-Myc and its downstream effectors, as well as apoptosis-associated genes, was analyzed using Western blot.
MINCR expression was increased in NSCLC patients from TCGA datasets, and was also significantly increased in our collected specimens from NSCLC patients and NSCLC cell lines. Knocking down of MINCR greatly inhibited the growth of NSCLC cell lines PC9 and A549. In addition, silencing of MINCR induced cell cycle arrest and apoptosis. Furthermore, silencing of MINCR reduced the expression levels of oncogene c-Myc and its downstream cyclin A, cyclin D, CD4, and CDK2, as well as apoptosis-associated Bcl-2, while significantly increased the expression levels of cleaved PARP-1. In the meantime, overexpression of MINCR remarkably enhanced cell proliferation of PC9 cells and activated c-Myc and its downstream effectors.
MINCR exerted inhibitory effects on the cell cycle arrest and apoptosis of NSCLC cells by activating c-Myc and its downstream effectors, suggesting that this lncRNA could be used as a potential therapeutic target for the treatment of NSCLC.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Ambrosia trifida is an invasive alien plant species, which has very high reproductive and environmental adaptability. Through strong resource acquisition ability and allelopathy, it could inhibit the ...growth and reproduction of surrounding plants and destroy the stability of an invasive ecosystem. It is very important to predict the change of suitable distribution area of A. trifida with climate change before implementing scientific control measures. Based on 106 A. trifida distribution data and 14 points of environmental data, the optimal parameter combination (RM = 0.1, FC = LQ) was obtained using the MaxEnt (version 3.4.1) model optimized by Kuenm package, and thus the potential suitable areas of A. trifida in Northeast China under three different climate scenarios (RCP2.6, RCP4.5, RCP8.5) with different emission intensities in the future (2050, 2070) were predicted. The changes of A. trifida suitable area in Northeast China under three climate scenarios were compared, and the relationship between the change of suitable area and emission intensity was analyzed. In general, the suitable area of A. trifida in Northeast China will expand gradually in the future, and the area of its highly suitable area will also increase with the increasing emission intensity, which is unfavorable to the control of A. trifida.
The advancement of Li-metal batteries is significantly impeded by the presence of unstable solid electrolyte interphase and Li dendrites upon cycling. Herein, we present an innovative approach to ...address these issues through the synergetic regulation of solid electrolyte interphase mechanics and Li crystallography using yttrium fluoride/polymethyl methacrylate composite layer. Specifically, we demonstrate the in-situ generation of Y-doped lithium metal through the reaction of composite layer with Li metal, which reduces the surface energy of the (200) plane, and tunes the preferential crystallographic orientation to (200) plane from conventional (110) plane during Li plating. These changes effectively passivate Li metal, thereby significantly reducing undesired side reactions between Li and electrolytes by 4 times. Meanwhile, the composite layer with suitable modulus (~1.02 GPa) can enhance mechanical stability and maintain structural stability of SEI. Consequently, a 4.2 Ah pouch cell with high energy density of 468 Wh kg
and remarkable capacity stability of 0.08% decay/cycle is demonstrated under harsh condition, such as high-areal-capacity cathode (6 mAh cm
), lean electrolyte (1.98 g Ah
), and high current density (3 mA cm
). Our findings highlight the potential of reactive composite layer as a promising strategy for the development of stable Li-metal batteries.
Morphology‐controlled nanostructures are important functional materials in multidisciplinary research fields, such as sensing, imaging, catalysis, and energy harvesting. However, existing ...nanofabrication methods have limitations in terms of controllability, diversity, cost, and efficiency. Here, a novel nanofabrication approach is proposed to construct Au nanostructures with high controllability, cost‐efficiency, and high‐performance sensing and energy harvesting. The fabrication incorporates electric field‐assisted hydrothermal growth of zinc oxide nanorods (ZnO NRs) with two‐step gold electroplating. The ZnO NRs serve as a dissolvable template during the electroplating formation of gold nanostructures for eventually synthesizing pure gold nanostructures on electrodes. The synthesized gold nanostructures take unique composite shape of nanospheres (hundreds of nanometers) covered with tinier nanostructures (tens of nanometers) like floccules, grains, lamellas, or rods, which are highly controlled by regulating electroplating parameters. The gold composite nanostructures provide high‐performance sensing and energy harvesting, such as enhanced electrochemical sensing interface (electric double‐layer impedance decrease up to 97.1%), excellent surface‐enhanced Raman scattering (SERS enhancement factor up to 5×107$5 \times \left(10\right)^{7}$), and preferable visible–near‐infrared light harvesting (absorptivity near to 90%). For the first time, the application of tumor cell photothermal therapy with nanostructured electrodes and in situ bimodal monitoring of single‐cell impedance and SERS‐based molecular expression during therapy is demonstrated.
A novel nanofabrication approach to controllably construct diverse gold composite nanostructures is proposed, enabling high‐performance electrochemical sensing and excellent Raman scattering measurement as well as preferable visible–near‐infrared light harvesting. For the first time, cytopathological change in tumor cell photothermal therapy is traced by in situ bimodal monitoring of single‐cell electrophysiology and surface‐enhanced Raman scattering‐based molecular expression.
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