Fluorescent Cd metal–organic frameworks (MOFs), Cd2(dicarboxylate)2(NI‐bpy‐44)2 (dicarboxylate=benzene‐1,4‐dicarboxylate (1,4‐bdc, 1), 2‐bromobenzene‐1,4‐dicarboxylate (Br‐1,4‐bdc, 2), ...2‐nitrobenzene‐1,4‐dicarboxylate (NO2‐1,4‐bdc, 3), biphenyl‐4,4′‐dicarboxylate (bpdc, 4); NI‐bpy‐44=N‐(pyridin‐4‐yl)‐4‐(pyridin‐4‐yl)‐1,8‐naphthalimide)), featuring non‐ and twofold interpenetrating pcu‐type bipillared‐layer open structures with sufficient free voids of 58.4, 51.4, 51.5, and 41.4 %, respectively, have been hydro(solvo)thermally synthesized. MOFs 1–4 emitted solid‐state blue or cyan fluorescence emissions at 447±7 nm, which mainly arose from NI‐bpy‐44 and are dependent on the incorporated solvents. After immersing the crystalline samples in different solvents, that is, H2O and DMSO (1 and 2) as well as nitrobenzene and phenol (1–4), they exhibited a remarkable fluorescence quenching effect, whereas o‐xylene and p‐xylene (4) caused significant fluorescence enhancement. The sensing ability of MOFs 1–4 toward nitro compounds carried out in the vapor phase showed that nitrobenzene and 2‐nitrophenol displayed detectable fluorescence quenching with 1, 2, and 4 whereas 4‐nitrotoluene was an effective fluorescence quencher for 1 and 2; this is most likely attributed to their electron‐deficient properties and higher vapor pressures. Moreover, MOFs 1–4 are highly reusable for quick capture of volatile iodine, as supported by clear crystal color change and also by immense fluorescence quenching responses owing to the donor–acceptor interaction. Low‐pressure CO2 adsorption isotherms indicate that activated materials 1′–4′ are inefficient at taking up CO2.
Cube root: The synthesis and crystal structures of four fluorescent Cd metal–organic frameworks (MOFs), Cd2(dicarboxylate)2(NI‐bpy‐44)2 (dicarboxylate=benzene‐1,4‐dicarboxylate (1,4‐bdc, 1), 2‐bromobenzene‐1,4‐dicarboxylate (Br‐1,4‐bdc, 2), 2‐nitrobenzene‐1,4‐dicarboxylate (NO2‐1,4‐bdc, 3), biphenyl‐4,4′‐dicarboxylate (bpdc, 4); NI‐bpy‐44=N‐(pyridin‐4‐yl)‐4‐(pyridin‐4‐yl)‐1,8‐naphthalimide), featuring a pcu‐type bipillared‐layer open framework, and their potential for sensing small organic molecules and iodine capture are reported.
Feline coronavirus (FCoV) can cause either asymptomatic enteric infection or fatal peritonitis in cats. Although the mutation of FCoV accessory gene 3c has been suggested to be related to the ...occurrence of feline infectious peritonitis (FIP), how the 3C protein is involved in this phenomenon remains unknown. To investigate the role of the 3C protein, a full-length 3c gene was transiently expressed and the cytoplasmic distribution of the protein was found to be primarily in the perinuclear region. Using 3c-stable expression cells, the replication of a 3c-defective FCoV strain was titrated and a significant decrease in replication (p<0.05) was observed. The mechanism underlying the decreased FIPV replication caused by the 3C protein was further investigated; neither the induction nor inhibition of autophagy rescued the viral replication. Taken together, our data suggest that the 3C protein might have a virulence-suppressing effect in FCoV-infected cats. Deletion of the 3c gene could therefore cause more efficient viral replication, which leads to a fatal infection.
碩士
國立暨南國際大學
應用化學系
105
A series of fluorescent metal-organic frameworks (MOFs) based on Cd(II) and N-(pyridin-4-yl)-4-(pydrin-4-yl)-1,8-naphthalimide (NI-bipy-44) with different aromatic ...dicarboxylates were synthesized. Cd2(1,4-bdc)2(NI-bipy-44)2 (1, 1,4-H2bdc = 1,4-benzenedicarboxylic acid), Cd2(1,4-bdc-Br)2(NI-bipy-44)2 (2, 1,4-H2bdc-Br = 2-bromoterephthalic acid), Cd2(1,4-bdc-NO2)2(NI-bipy-44)2 (3, 1,4-H2bdc-NO2 = 2-nitroterephthalic acid), and Cd2(BPDC)2(NI-bipy-44)2 (4, H2BPDC = biphenyl-4,4′-dicarboxylic acid) have similar structures suiting a 3D pillared-layer framework with porosity of 58.4%, 51.4%, 51.5%, and 41.4%, respectively. Of note, compound 4 is 2-fold interpenetration, while compounds 1-3 are non-interpenetration.
Compounds 1-4 exhibit intense solid-state photoluminescence with λmax of 450 ± 5 nm, making them useful for detecting small organic molecules and nitro compounds.
When compounds 1 and 2 were soaked in H2O and DMSO, there was a significant fluorescence quenching. On the other
Diosgenin, a traditional Yam extraction, has been used in hormone replacement for menopausal women. We aimed to investigate the influences of diosgenin administration upon the MMP-2 and -9 activity ...and expression and reproductive hormones of ovariectomized (OVX) rats, a model of menopausal status. Seven-week old female Wistar rats with bilateral OVX or sham operation (controls) were divided and administered different dosages of diosgenin (0, 10, 50, or 100 mg/kg/day) for 8 weeks. Serum was then sampled for progesterone (P4) and estradiol (E2) assay and uterine horns harvested. Myometrial MMP-2 and -9 activity and expression were surveyed and myometrial collagen expression was also assayed. The results show higher body weight in OVX rats across the 8 weeks post surgery and no significant differences were noted among OVX or Sham rats with diosgenin supplements. There were lower P4 and E2 concentrations in OVX rats compared to Sham rats, and higher P4 concentration of Sham rats post diosgenin supplement. MMP-2 and -9 mRNA expression and activity was lower in OVX rats, although higher MMP-2 and lower MMP-9 activity/mRNA expression was observed in OVX rats post diosgenin supplementation. Collagen mRNA expression was higher in OVX rats compared to Sham controls, and diosgenin administration decreased collagen mRNA expression in OVX rats. In conclusion, diosgenin is associated with gelatinase expression and collagen metabolism in OVX rats. Diosgenin administration can partially reverse the effects of OVX upon MMP functions and hormone status. Adequate diosgenin supplement might modulate myometrial gelatinase expression and collagen metabolism in menopausal subjects.
The extracellular matrix (ECM) plays a critical role in the development and invasion of primary breast tumors. Lysyl oxidase (LOX), which is an ECM remodeling enzyme, appears to play roles in ...promoting cancer cell motility and invasion. To ascertain whether LOX overexpression in breast tumor tissues from Asian patients is associated with decreases in metastasis-free and overall survival in breast cancer patients, the mRNA levels of LOX were examined in paired tumor/normal tissue samples using real-time RT-PCR analysis (
n
= 246 pair-matched samples). To test whether specifically targeting LOX by inhibiting its activity (using beta-aminopropionitrile (β-APN), a LOX inhibitor), mRNA expression (using siRNA), or protein expression (using 25 μM magnolol) attenuates the invasion of MDA-MB-231 breast cancer cells, a cancer cell migration assay was performed. Interestingly, only 78.5% (
n
= 193) of the breast cancer tumors displayed detectable LOX expression. Nearly 60% (
n
= 120) of the cases fell into Group 1 (tumor > normal,
T
>
N
); in this group, the mean LOX expression in the tumor cells was 20.2-fold greater than in normal cells. However, in Group 2 (normal > tumor,
N
>
T
), the LOX expression level in most of the normal tissues examined (80%, 59/73) was less than fivefold greater than in the tumor tissues. The increased level of active LOX in the invasive breast cancer cell line MDA-MB-231 was accompanied by the increased phosphorylation of focal adhesion kinase at Tyr-576 and of paxillin at Tyr-118. We also found that the addition of β-APN (300 μM) and magnolol (25 μM), synergistically inhibited the migration and invasion of MDA-MB-231 cells. In this article, we describe, for the first time, higher expression of a LOX protein in breast tumors compared with normal tissues from Asian patients. Moreover, the results indicate that the inhibition of LOX using magnolol may represent a more desirable strategy for breast cancer therapy than the use of β-APN.
Pleural effusion is common problem, but the rapid and reliable diagnosis for specific pathogenic effusions are lacking. This study aimed to identify the diagnosis based on clinical variables to ...differentiate pleural tuberculous exudates from other pleural effusions. We also investigated the role of renin-angiotensin system (RAS) and matrix metalloproteinase (MMPs) in the pathogenesis of pleural exudates.
The major components in RAS and extracellular matrix metabolism, including angiotensin converting enzyme (ACE), ACE2, MMP-2 and MMP-9 activities, were measured and compared in the patients with transudative (n = 45) and exudative (n = 80) effusions. The exudative effusions were come from the patients with tuberculosis (n = 20), pneumonia (n = 32), and adenocarcinoma (n = 28).
Increased ACE and equivalent ACE2 activities, resulting in a significantly increased ACE/ACE2 ratio in exudates, were detected compared to these values in transudates. MMP-9 activity in exudates was significantly higher than that in transudates. The significant correlation between ACE and ACE2 activity that was found in transudates was not found in exudates. Advanced analyses showed significantly increased ACE and MMP-9 activities, and decreased ACE2 activity in tuberculous pleural effusions compared with those in pneumonia and adenocarcinoma effusions. The results indicate that increased ACE and MMP-9 activities found in the exudates were mainly contributed from a higher level of both enzyme activities in the tuberculous pleural effusions.
Interplay between ACE and ACE2, essential functions in the RAS, and abnormal regulation of MMP-9 probably play a pivotal role in the development of exudative effusions. Moreover, the ACE/ACE2 ratio combined with MMP-9 activity in pleural fluid may be potential biomarkers for diagnosing tuberculous pleurisy.
This paper develops a novel ultra-wideband bandpass filter by cascading a broadband bandpass filter with another broadband bandstop filter. Properly selected impedances of transmission lines achieve ...broadband bandpass and bandstop filters and make independent designs possible. Detailed design and synthesis procedures are provided; moreover, agreement between measured and theoretically predicted results demonstrates feasibility of the proposed filter. Due to its simple structure, the ultra-wideband bandpass filter newly introduced in this paper is suitable for integration in the single-chipped circuit or implementation on printed circuit boards.
A 3-dB planar wideband power divider with an ultra-wide stopband and isolated frequency band is proposed. In order to extend the passband, the quasi-coupled lines are adopted. Moreover, the open and ...shorted stubs added at the input and output ports, respectively, can increase the bandwidths of the passband and stopband. Furthermore, in order to broaden the isolated frequency band, the impedance Z iso with the series connected resister and capacitor on the right side of the middle coupled line is adopted. The detailed derivation is proposed as well. In addition, this power divider is fabricated on the substrate Rogers RO4003C with a compact size of 15.19 mm × 11.4 mm.
PDF
