This article reviews current co-culture systems for fermenting mixtures of glucose and xylose to ethanol. Thirty-five co-culture systems that ferment either synthetic glucose and xylose mixture or ...various biomass hydrolysates are examined. Strain combinations, fermentation modes and conditions, and fermentation performance for these co-culture systems are compared and discussed. It is noted that the combination of Pichia stipitis with Saccharomyces cerevisiae or its respiratory-deficient mutant is most commonly used. One of the best results for fermentation of glucose and xylose mixture is achieved by using co-culture of immobilized Zymomonas mobilis and free cells of P. stipitis, giving volumetric ethanol production of 1.277 g/l/h and ethanol yield of 0.49-0.50 g/g. The review discloses that, as a strategy for efficient conversion of glucose and xylose, co-culture fermentation for ethanol production from lignocellulosic biomass can increase ethanol yield and production rate, shorten fermentation time, and reduce process costs, and it is a promising technology although immature.
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Dostopno za:
CEKLJ, DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Vascular endothelial dysfunction, a characteristic of the aging process, is an important risk factor for cardiovascular disease in aging. Although, vascular inflammation and oxidative stress are ...major contributors to endothelial dysfunction in aging, the underlying mechanisms during the aging process are not fully understood. Accumulating evidence reveals that gut microbiota-dependent metabolite trimethylamine-N-oxide (TMAO) is implicated in the pathogenesis of many cardiovascular diseases. We tested the hypothesis that aging increases circulating TMAO levels, which induce vascular inflammation and oxidative stress, resulting in age-associated endothelial dysfunction. Old (22-mo-old) and young (4-mo-old) Fischer-344 rats were treated without (control) or with 1.0% 3,3-Dimethyl-1-butanol (DMB, an inhibitor of trimethylamine formation) in drinking water for 8 weeks. Compared with young control group, old control group had markedly higher plasma TMAO levels, which were reduced by DMB treatment. Endothelium-dependent relaxation of aorta in response to acetylcholine was impaired in old control group compared with young control group as indicated by decreased maximal relaxation (E
) and reduced area under the curve (AUC). E
and AUC were both normalized in old rats treated with DMB. No difference in endothelial-independent relaxation in response to sodium nitroprusside was observed among groups. Molecular studies revealed that old control group exhibits increased expression of proinflammatory cytokines and superoxide production, and decreased expression of endothelial nitric-oxide synthase (eNOS) in the aorta, all of which were restored by DMB treatment. These results suggest that aging increases circulating TMAO levels, which may impair eNOS-derived NO bioavailability by increasing vascular inflammation and oxidative stress, contributing to aging-associated endothelial dysfunction.
The ever‐increasing demand for large‐scale energy storage systems requires novel battery technologies with low‐cost and sustainable properties. Due to earth‐abundance and cost effectiveness, the ...development of rechargeable potassium ion batteries (PIBs) has recently attracted much attention. Since carbon‐based materials are abundant, inexpensive, nontoxic, and safe, extensive feasibility investigations have suggested that they can become promising anode materials for PIBs. This review not only attempts to provide better understanding of the potassium storage mechanism, but also summarizes the availability of new carbon‐based materials and their electrochemical performance covering graphite, graphene, and hard carbon materials plus carbon‐based composites. Finally, the critical issues, challenges, and perspectives are discussed to demonstrate the developmental direction of PIBs.
This review not only attempts to provide better understanding of the potassium storage mechanism, but also summarizes the availability of new carbon‐based materials and their electrochemical performance, covering graphite, graphene, and hard carbon materials plus carbon‐based composites. Finally, the critical issues, challenges, and perspectives are discussed to demonstrate the developmental direction of Potassium‐Ion Batteries.
Chronic kidney disease (CKD) is strongly associated with increased cardiovascular risk. Impaired endothelial function, a key initiating step in the pathogenesis of cardiovascular disease, has been ...reported in patients with CKD, but the mechanisms responsible for endothelial dysfunction in CKD remain elusive. Emerging evidence reveals that trimethylamine-N-oxide (TMAO), a gut microbiota-generated metabolite, is involved in the pathogenesis of many cardiovascular diseases. Circulating TMAO is elevated in CKD. Here we tested the hypothesis that elevated TMAO plays a contributory role in the pathogenesis of endothelial dysfunction in CKD. Rats underwent 5/6 nephrectomy to induce CKD or sham operation, and were treated with 1.0% 3,3-Dimethyl-1-butanol (DMB, an inhibitor of trimethylamine formation) or vehicle. Eight weeks after nephrectomy and DMB treatment, circulating TMAO levels were markedly elevated in CKD-vehicle rats compared with sham-vehicle rats, but were reduced in CKD-DMB rats. Acetylcholine-induced endothelium-dependent vasodilation was impaired in CKD-vehicle rats compared with sham-vehicle rats as indicated by reduced maximal relaxation (Emax) and decreased area under the curve (AUC). Emax and AUC were both normalized in CKD-DMB rats. No difference in sodium nitroprusside-induced endothelial-independent vasodilation was observed across groups. Molecular studies revealed that endothelial nitric-oxide synthase activity was decreased, while superoxide production and proinflammatory cytokine expression were increased in the aorta of CKD-vehicle rats compared with sham-vehicle rats. Of note, the abnormalities in above molecular parameters were completely restored in CKD-DMB rats. These results suggest that CKD elevates circulating TMAO levels, which may reduce eNOS-derived NO production by increasing vascular oxidative stress and inflammation, contributing to CKD-associated endothelial dysfunction and cardiovascular disease.
•Circulating TMAO is elevated in chronic kidney disease.•Elevated TMAO in chronic kidney disease impairs endothelial function.•Elevated TMAO in chronic kidney disease reduces vascular eNOS activity.•Elevated TMAO in chronic kidney disease causes vascular oxidative stress.•Elevated TMAO in chronic kidney disease induces vascular inflammation.
Developing nonprecious hydrogen evolution electrocatalysts that can work well at large current densities (e.g., at 1000 mA/cm2: a value that is relevant for practical, large-scale applications) is of ...great importance for realizing a viable water-splitting technology. Herein we present a combined theoretical and experimental study that leads to the identification of α-phase molybdenum diboride (α-MoB2) comprising borophene subunits as a noble metal-free, superefficient electrocatalyst for the hydrogen evolution reaction (HER). Our theoretical finding indicates, unlike the surfaces of Pt- and MoS2-based catalysts, those of α-MoB2 can maintain high catalytic activity for HER even at very high hydrogen coverage and attain a high density of efficient catalytic active sites. Experiments confirm α-MoB2 can deliver large current densities in the order of 1000 mA/cm2, and also has excellent catalytic stability during HER. The theoretical and experimental results show α-MoB2’s catalytic activity, especially at large current densities, is due to its high conductivity, large density of efficient catalytic active sites and good mass transport property.
Path planning is important to the efficiency and navigation safety of USV autonomous operation offshore. To improve path planning, this study proposes the improved ant colony optimization-artificial ...potential field (ACO-APF) algorithm, which is based on a grid map for both local and global path planning of USVs in dynamic environments. The improved ant colony optimization (ACO) mechanism is utilized to search for a globally optimal path from the starting point to the endpoint for a USV in a grid environment, and the improved artificial potential field (APF) algorithm is subsequently employed to avoid unknown obstacles during USV navigation. The primary contributions of this article are as follows: (1) this article proposes a new heuristic function, pheromone update rule, and dynamic pheromone volatilization factor to improve convergence and mitigate finding local optima with the traditional ant colony algorithm; (2) we propose an equipotential line outer tangent circle and redefine potential functions to eliminate goals unreachable by nearby obstacles (GNRONs) and local minimum problems, respectively; (3) to adapt the USV to a complex environment, this article proposes a dynamic early-warning step-size adjustment strategy in which the moving distance and safe obstacle avoidance range in each step are adjusted based on the complexity of the surrounding environment; (4) the improved ant colony optimization algorithm and artificial potential field algorithm are effectively combined to form the algorithm proposed in this article, which is verified as an effective solution for USV local and global path planning using a series of simulations. Finally, in contrast to most papers, we successfully perform field experiments to verify the feasibility and effectiveness of the proposed algorithm.
Recycling precious metals from electronic waste not only benefits environmental protection, but is also favorable for alleviating resource shortages. Ionic porous organic polymers, as one type of ...burgeoning material, are regarded as excellent adsorbents due to their high ion density, but their application in precious metal recovery is still very limited. Here, V-PPOP-Br, a highly stable and easy-to-build cationic porous organic polymer, was successfully prepared for the first time. By linking porphyrins with viologens, V-PPOP-Br obtained the characteristics of a hierarchical porous structure, a high ion density skeleton, and a rich nitrogen content, which gave it an ultrahigh adsorption capacity (Qmax = 792.22 mg g−1) and rapid adsorption rate for Au(III). V-PPOP-Br also had an effective Au(III) recovery capability from SIM cards. Mechanism investigation confirmed that this remarkable adsorption performance was attributed to the interplay of ion exchange, redox reactions and coordination. Moreover, V-PPOP-Br had excellent recyclability and could maintain an ultrahigh adsorption efficiency of 81% after eight consecutive adsorption-desorption experiments. These excellent performances as well as the roughly calculated gold recycling economics ($37.37/g V-PPOP-Br) confirmed that it possesses promising potential as an ionic porous material for gold recovery.
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•A porphyrin and viologen-linked cationic POP was prepared via Zincke reaction.•The V-PPOP-Br exhibited ultrahigh adsorption capacity for Au(III).•The mechanism was ascribed to the ion exchange, redox reaction, and coordination.•The V-PPOP-Br could selectively recover gold from the SIM card.•The V-PPOP-Br possessed great recyclability and practical application potential.
ABSTRACT
ETHYLENE RESPONSE FACTOR1 (ERF1) is a key component in ethylene signaling, playing crucial roles in both biotic and abiotic stress responses. Here, we demonstrate that ERF1 also has an ...important role during floral initiation in Arabidopsis thaliana. Knockdown or knockout of ERF1 accelerated floral initiation, whereas overexpression of ERF1 dramatically delayed floral transition. These contrasting phenotypes were correlated with opposite transcript levels of FLOWERING LOCUS T (FT). Chromatin immunoprecipitation (ChIP) assays revealed that ERF1 associates with genomic regions of the FT gene to repress its transcription. ft‐10/ERF1RNAi plants showed a similar flowering phenotype to the ft‐10 mutant, whereas the flowering of FTox/ERF1ox mimicked that of FTox plants, suggesting that ERF1 acts upstream of FT during floral initiation. Similarly, altered floral transition in ethylene‐related mutants was also correlated with FT expression. Further analysis suggested that ERF1 also participates in delay in flowering‐time control mediated by the ethylene precursor 1‐aminocyclopropane‐1‐carboxylic acid. Thus, ERF1 may act as a negative modulator of flowering‐time control by repressing FT transcription in Arabidopsis.
Flowering plants have evolved sophisticated gene regulatory networks to coordinately govern floral initiation under suitable environmental conditions. ETHYLENE RESPONSE FACTOR1 functions as a negative modulator in the transmission of ethylene‐mediated flowering signals by directly associating with the FLOWERING LOCUS T promoter and repressing FLOWERING LOCUS T expression.
Silicon is regarded as one of the most promising candidates for lithium-ion battery anodes owing to its large theoretical energy density (about 4200 mAh g−1) and low working potential (vs. Li/Li+). ...However, its practical application is limited by structure degradation and a comparatively poor capacity retention caused by large volume changes during cycling. In this study, we have prepared a novel nanofiber form of silicon/carbon with hollow core–shell structured silicon@carbon (Si@C) nanoparticles embedded in carbon nanofibers. Voids between the silicon nanoparticle (SiNP) core and carbon shell help to accommodate the volume expansion associated with the lithiation/delithiation process in a working electrode and allow formation of a stable solid electrolyte interphase (SEI) film. The obtained electrodes exhibited good cycle performance with a high reversible capacity of 1020.7 mAh g−1 after 100 cycles at a current density of 0.2 A g-1, and also delivered excellent cycling performance at a high current density of 3.2 A g-1. The design of this new structure provides a potential method for developing other functional composite anode materials with high reversible capacities and long-term cycle stabilities.
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•Core-shell-structured Si@SiO2 nanoparticles were prepared by a sol−gel method.•The hollow core-shell structure was formed by electrospinning and an HF treatment.•The void could accommodate the volume expansion of Si nanoparticles.•A stable solid electrolyte interface film formed on the carbon shell surface.•The structural integrity and cycle stability were significantly improved.
The urgent need for sustainable energy development depends on the progress of green technologies, which have steered hot research areas into environmentally benign approaches viainexpensive ...precursors and abundant resources obtained directly from nature for energy devices such as fuel cells and supercapacitors. By using fermented rice as starting materials, we herein demonstrate a facile, green and scalable approach to synthesize porous N-doped carbon spheres characterised by high specific surface areas (2105.9 m super(2) g super(-1)) and high porosity (1.14 cm super(3) g super(-1)), which exhibit not only excellent electrocatalytic activity toward the four-electron oxygen reduction reaction with long-term stability for fuel cells, but also have excellent resistance to crossover effects and CO poisoning superior to that of the commercial Pt/C catalyst. Furthermore, the naturally derived porous N-doped carbon spheres, used as the active electrode materials, present superior performance for capacitors with a capacitance of 219 F g super(-1) at a high discharge current density of 15 A g super(-1) and good cycling stability for over 4400 cycles. This work shows a good example for taking advantage of the abundant resources provided by nature, and opening the door for the creation of functional materials with promising applications in high-performance renewable devices related to energy conversion and storage.
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