T-cell immunoglobulin and mucin domain 3 (TIM-3) belongs to the group of inhibitory checkpoint receptors and has traditionally been of interest in terms of its expression on activated CD4
+
and CD8
+
...T cells. The treatment with TIM-3 inhibitors is considered as a promising strategy in cancer immunotherapy. The review focuses on new data on the expression of TIM-3 on dendritic cells (DCs) that play a key role in initiating the antigen-specific immune response and inducing effector CD8
+
T cells. The main hypothesis is that TIM-3 is suggested to act as a negative regulator of DCs. Further studies on TIM-3-mediated DC regulation will improve the effectiveness of current strategies in the treatment of cancer using DCs and checkpoint molecule inhibitors, where the main targets can be not only T cells, but also TIM-3-expressing DCs.
Hole traps in hydride vapor phase epitaxy β-Ga2O3 films were studied by deep level transient spectroscopy with electrical and optical excitation (DLTS and ODLTS) and by photocapacitance and ...temperature dependence measurements. Irradiation with 20 MeV protons creates deep electron and hole traps, a strong increase in photocapacitance, and prominent persistent photocapacitance that partly persists above room temperature. Three hole-trap-like signals H1 self-trapped holes (STH), H2 electron capture barrier (ECB), and H3, with activation energies 0.2 eV, 0.4 eV, 1.3 eV, respectively, were detected in ODLTS. The H1 (STH) feature is suggested to correspond to the transition of polaronic states of STH to mobile holes in the valence band. The broad H2 (ECB) feature is due to overcoming of the ECB of the centers responsible for persistent photocapacitance for temperatures below 250 K. The H3 peak is produced by detrapping of holes from Ev + 1.3 eV hole traps believed to be related to gallium vacancy acceptors. One more deep acceptor with optical ionization threshold near 2.3 eV is likely responsible for high temperature persistent photocapacitance surviving up to temperatures higher than 400 K. The latter traps show a significant barrier for capture of electrons.
We studied angiogenin production by human macrophages and evaluated the role of this factor in the macrophage-mediated regulation of fibroblasts. All macrophage subtypes, and especially the ...efferocytosis-polarized macrophages, M2(LS), actively produced angiogenin. Exogenous recombinant angiogenin dose-dependently enhanced the proliferation and differentiation of dermal fibroblasts. The addition of the angiogenin inhibitor to fibroblasts cultures suppressed the stimulating effect of exogenous angiogenin or M2(LS) conditioned media. These findings indicate the involvement of angiogenin in the macrophage-mediated paracrine regulation of skin fibroblasts.
We studied suppressor potential of myeloid-derived suppressor cells (MDSC) in multiple myeloma patients, including before and after mobilization of hematopoietic stem cells (HSC), by evaluating the ...expression of arginase-1 (Arg1), indolamine-2,3-dioxygenase (IDO), and PD-L1 in MDSC subsets. The study included 20 multiple myeloma patients in remission, 5 patients with progression, as well as 10 sex-and age-matched healthy donors. The expression of Arg1, IDO, and PD-L1 in circulating granulocytic MDSC (G-MDSC, Lin
–
HLA-DR
–
CD33
+
CD66b
+
), monocytic MDSC (M-MDSC, CD14
+
HLA-DR
low/–
), and early-stage MDSC (E-MDSC, Lin
–
HLA-DR
–
CD33
+
CD66b
–
) was evaluated by flow cytometry. Multiple myeloma patients in remission were characterized by reduced expression of Arg1 in M-MDSC in comparison with donors. The expression of Arg1 in M-MDSC depended on the number of induction therapy lines performed and was significantly lower in patients who received ⩾2 lines and responded with remission. Patients with multiple myeloma progression (resistant to therapy) showed significantly increased expression of Arg1 and PD-L1 in M-MDSC, as well as increased expression of Arg1 in E-MDSC. After G-CSF-induced mobilization of HSC, the content of circulating Arg1-expressing M-MDSC increased significantly. Considering the presence of MDSC in apheresis products, MDSC suppressive activity is discussed as a factor affecting the outcomes of autologous HSC transplantation in multiple myeloma patients.
Abstract Neurorehabilitation of poststroke cognitive impairments is an important medical problem. The purpose of the present study was to estimate the efficacy of new methods of restoration of ...impaired cognitive functions using computer correction programs. 43 poststroke patients aged 57–69, (male — 23, female — 20) were randomized into two groups. First group patients have been treated with the standard methods and supplementary neuropsychological computer training for 14 days, 25–35 min of duration per day. Control group received standard treatment according to Federal and local medical recommendations. Initial and achieved levels of cognitive functioning were estimated with the use of Mini Mental State Examination, Frontal Assessment Battery, the Clock Drawing Test, the Montreal Cognitive Assessment, Schulte's test, Hospital Anxiety and Depression Scale. We found that including the computer correction programs into the complex protocol of rehabilitation of post-stroke patients confirmed their efficacy in both clinical aspects and the Patient Global Impression Scale. Although the results are encouraging, further studies are required with larger samples and longer follow-up to identify characteristics of those patients who are most likely to benefit from computer training of cognitive functions.
We studied the expression of arginase-1 (Arg1) and tyrosine kinase Mer (MerTK) in GMCSF-differentiated human macrophage populations М0, М1(IFNγ), М2а(IL-4), and М2(low serum) generated under ...conditions of growth/serum factor deficiency. The maximum relative content of Arg1+ and MerTK+ cells was found in М2 macrophage populations: М2а(IL-4) and М2(low serum). As the uptake of apoptotic cells is the key mechanism of M2 polarization during M2(low serum) generation, we performed a special series of experiments and showed that incubation with allogeneic apoptotic neutrophils significantly increased the percentages of CD206+ macrophages co-expressing Arg1 and MerTK.
The atomic and electronic structure of intrinsic point defects in orthorhombic tantalum oxide has been studied by numerical simulation within the density functional theory. It has been shown that all ...defects responsible for metal enrichment of Ta
2
O
5
serve as electron and hole traps. Under conditions of strong oxygen depletion and at a metal–insulator interface, which are characteristic of resistive memory elements, interstitial tantalum atoms compete with an oxygen vacancy in the formation of a conducting filament. Interstitial oxygen atoms are not involved in charge transport. Tantalum substituting oxygen can be considered as a combination of the oxygen vacancy and interstitial tantalum. The analysis of the calculated thermal and optical energies of trap ionization shows that the oxygen vacancy is a key defect for charge transport in Ta
2
O
5
.
The NICA accelerator complex includes beam transfer lines and stations for applied research. The first commissioning of the Station of Chip Irradiation (SOCHI) was performed at the end of 2021with С
...4+
heavy ions extracted from the linear accelerator (HILAC) at an energy of 3.2 MeV/n. The new SOCHI beam transfer line is integrated in the existing HILAC-Booster beamline. The Irradiation Setup for Components of Radioelectronic Apparatus (ISCRA) with ion energy ranging from 150 to 500 MeV/n and the Setup for Investigation of Medical Biological Objects (SIMBO) with the ion energy ranging from 400 to1100 MeV/n are based on the beams extracted from Nuclotron. The equipment of ISCRA and SIMBO stations has been manufactured and is planned to mount in the end of 2022. The beamlines are being designed now. The technical parameters of the beamlines and stations and the results of the first run of the SOCHI station are presented in this study.
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