Organic UV-filters are key ingredients found in sunscreens, cosmetics and plastic goods. Concerns have been raised about potential ecological and human health effects of certain organic UV filters ...that are currently FDA approved for use in the United States. Here, we investigate the photochemical fate of two of these compounds, oxybenzone and sulisobenzone. Both oxybenzone and sulisobenzone have previously been detected in surface waters, seawater, and treated wastewater effluent. Direct photolysis is found to be unimportant for these compounds, however, enhanced photodegradation of oxybenzone and sulisobenzone was observed under simulated solar irradiation in solutions of International Humic Substance Society standards (Pony Lake fulvic acid and Suwannee River natural organic matter), filtered wastewater effluent (Southerly Wastewater Treatment Plant in Lockbourne, OH), and Scioto River water (Columbus, OH) at pH 7. Quenching experiments with isopropanol revealed that the main pathway for degradation appears to be reaction with the hydroxyl radical (OH). Observed degradation rates were 2–3 times slower than estimates calculated using literature reported second-order rate constants and measured hydroxyl radical steady-state concentrations for Suwannee River natural organic matter, Pony Lake fulvic acid and Scioto waters. The Southerly sample, however, exhibited nearly identical expected and observed rate constants, which we take to indicate the presence of unidentified reactive species that can react with oxybenzone and sulisobenzone. Values obtained in this work were used to calculate second-order rate constants for oxybenzone and sulisobenzone with the hydroxyl radical, as well as to estimate environmental half-lives for these compounds. Near surface 24-h averaged half-lives of 3.0 and 4.0 days were calculated for oxybenzone and sulisobenzone, respectively. When extrapolated to an environmentally representative water column, these same 24-h averaged half-lives increased to 2.4 and 3.5 years, respectively.
•No direct photodegradation observed for oxybenzone.•Sulisobenzone undergoes slow direct photolysis (kdirect = 5.39 ± 0.28 × 10−7 s−1).•DOM addition accelerates oxybenzone and sulisobenzone photodegradation.•Primary degradation mechanism of oxybenzone and sulisobenzone by reaction with OH.•Freshwater half-lives estimated at 2.4 (oxybenzone) and 3.5 (sulisobenzone) years.
Skin cancer, a malignant neoplasm originating from skin cell types including keratinocytes, melanocytes, and sweat glands, comprises three primary forms: basal cell carcinoma (BCC), squamous cell ...carcinoma (SCC), and malignant melanoma (MM). BCC and SCC, while constituting the most prevalent categories of skin cancer, are generally considered less aggressive compared to MM. Notably, MM possesses a greater capacity for invasiveness, enabling infiltration into adjacent tissues and dissemination via both the circulatory and lymphatic systems. Risk factors associated with skin cancer encompass ultraviolet (UV) radiation exposure, fair skin complexion, a history of sunburn incidents, genetic predisposition, immunosuppressive conditions, and exposure to environmental carcinogens. Early detection of skin cancer is of paramount importance to optimize treatment outcomes and preclude the progression of disease, either locally or to distant sites. In pursuit of this objective, numerous computer-aided diagnosis (CAD) systems have been developed. Hyperspectral imaging (HSI), distinguished by its capacity to capture information spanning the electromagnetic spectrum, surpasses conventional RGB imaging, which relies solely on three color channels. Consequently, this study offers a comprehensive exploration of recent CAD investigations pertaining to skin cancer detection and diagnosis utilizing HSI, emphasizing diagnostic performance parameters such as sensitivity and specificity.
Sulfadimethoxine (SDM) is an antibiotic often used in combination with ormetoprim to prevent the spread of disease in freshwater aquaculture. It is known to undergo photochemical degradation in ...natural sunlit surface waters, but the role of dissolved organic matter (DOM) in this process is poorly understood. Our results show that water from a eutrophic catfish pond at the Mississippi State University Delta Research and Extension Center facility in Stoneville, MS facilitates the rapid phototransformation of SDM. In contrast, water from a nearby stream (Deer Creek) whose DOM is derived from allochthonous precursors does not enhance SDM photodegradation. We attribute these disparate results to DOM composition, whereby dissolved organic matter originating from highly eutrophic water bodies is a better SDM photosensitizer. Experiments conducted concurrently using respective autochthonous (Pony Lake, Antarctica) and allochthonous (Suwannee River) derived fulvic acids corroborate these findings. Scavenging experiments and experiments conducted anoxically show that the main indirect photodegradation pathway occurs by triplet excited-state DOM oxidation. Finally, transformation products assayed by mass spectrometry reveal the same major SDM photoproducts in the presence and absence of dissolved organic matter.
A-to-I RNA editing, catalyzed by the ADAR protein family, significantly contributes to the diversity and adaptability of mammalian RNA signatures, aligning with developmental and physiological needs. ...Yet, the functions of many editing sites are still to be defined. The Unc80 gene stands out in this context due to its brain-specific expression and the evolutionary conservation of its codon-altering editing event. The precise biological functions of Unc80 and its editing, however, are still largely undefined. In this study, we first demonstrated that Unc80 editing occurs in an ADAR2-dependent manner and is exclusive to the brain. By employing the CRISPR/Cas9 system to generate Unc80 knock-in mouse models that replicate the natural editing variations, our findings revealed that mice with the “gain-of-editing” variant (Unc80G/G) exhibit heightened basal neuronal activity in critical olfactory regions, compared to the “loss-of-editing” (Unc80S/S) counterparts. Moreover, an increase in glutamate levels was observed in the olfactory bulbs of Unc80G/G mice, indicating altered neurotransmitter dynamics. Behavioral analysis of odor detection revealed distinctive responses to novel odors—both Unc80 deficient (Unc80+/−) and Unc80S/S mice demonstrated prolonged exploration times and heightened dishabituation responses. Further elucidating the olfactory connection of Unc80 editing, transcriptomic analysis of the olfactory bulb identified significant alterations in gene expression that corroborate the behavioral and physiological findings. Collectively, our research advances the understanding of Unc80’s neurophysiological functions and the impact of its editing on the olfactory sensory system, shedding light on the intricate molecular underpinnings of olfactory perception and neuronal activity.
Osteoarthritis (OA) is still a recalcitrant musculoskeletal disease on account of its complex biochemistry and mechanical stimulations. Apart from stimulation by external mechanical forces, the ...regulation of intracellular mechanics in chondrocytes has also been linked to OA development. Recently, visfatin has received significant attention because of the clinical finding of the positive correlation between its serum/synovial level and OA progression. However, the precise mechanism involved is still unclear. This study determined the effect of visfatin on intracellular mechanics and catabolism in human primary chondrocytes isolated from patients. The intracellular stiffness of chondrocytes was analyzed by the particle-tracking microrheology method. It was shown that visfatin damages the microtubule and microfilament networks to influence intracellular mechanics to decrease the intracellular elasticity and viscosity via glycogen synthase kinase 3β (GSK3β) inactivation induced by p38 signaling. Further, microtubule network destruction in human primary chondrocytes is predominantly responsible for the catabolic effect of visfatin on the cyclooxygenase 2 upregulation. The present study shows a more comprehensive interpretation of OA development induced by visfatin through biochemical and biophysical perspectives. Finally, the role of GSK3β inactivation, and subsequent regulation of intracellular mechanics, might be considered as theranostic targets for future drug development for OA.
A recommendation system is based on the user and the items, providing appropriate items to the user and effectively helping the user to find items that may be of interest. The most commonly used ...recommendation method is collaborative filtering. However, in this case, the recommendation system will be injected with false data to create false ratings to push or nuke specific items. This will affect the user’s trust in the recommendation system. After all, it is important that the recommendation system provides a trusted recommendation item. Therefore, there are many algorithms for detecting attacks. In this article, it proposes a method to detect attacks based on the beta distribution. Different researchers in the past assumed that the attacker only attacked one target item in the user data. This research simulated an attacker attacking multiple target items in the experiment. The result showed a detection rate of more than 80%, and the false rate was within 16%.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
38.
Dupilumab bei einem 5‐jährigen Kind mit Prurigo nodularis Lin, Chia‐Jen; Li, Yun‐Chang; Chang, Hui‐Chin ...
Journal der Deutschen Dermatologischen Gesellschaft,
December 2023, 2023-12-00, Letnik:
21, Številka:
12
Journal Article
Photolysis of the non-steroidal anti-inflammatory drug ibuprofen was studied by exposure to a solar simulator in solutions of fulvic acid (FA) isolated from Pony Lake, Antarctica; Suwannee River, GA, ...USA; and Old Woman Creek, OH, USA. At an initial concentration of 10 μM, ibuprofen degrades by direct photolysis, but the presence of FA significantly increases reaction rates. These reactions proceeded up to 6× faster in FA solutions at lower ibuprofen concentrations (0.1 μM), but the rates are highly dependent upon DOM composition. Incomplete quenching of the reaction in the presence of isopropanol suggests that the hydroxyl radical is only partially responsible for ibuprofen’s photodegradation in FA solutions, and other reactive transients likely play an important role. Liquid chromatography-quadrupole time-of-flight mass spectrometry and NMR spectroscopy reveal the formation of multiple photoproducts, with three byproducts identified as 1-(4-isobutylphenyl)ethanol, isobutylacetophenone, and a phenol derivative. Pony Lake FA significantly increases the production of the major byproduct relative to yields produced by direct photolysis and the other two FA. Thus, the photolytic fate of ibuprofen in sunlit waters is affected by its initial concentration and the source of dissolved organic matter present.
► Ibuprofen photodegradation occurs slowly in water and is enhanced in the presence of fulvic acids. ► The composition of fulvic acids influences ibuprofen’s transformation pathway and kinetics. ► Enhanced photoreactivity of ibuprofen at low initial concentration (0.1 μM) in the presence of fulvic acids was observed. ► Multiple photoproducts were identified including isobutylacetophenone, a precursor used in the synthesis of ibuprofen.
Abstract
Background
Pentafecta is a major goal in the era of partial nephrectomy (PN). Simplified PADUA REnal (SPARE) nephrometry system was developed to evaluate the complexity of tumor. However, ...the predictive ability in pentafecta of SPARE system is yet to be determined. The aim of this study was to externally validate the applicability of SPARE nephrometry system in predicting pentafecta achievement after partial nephrectomy, and to examine inter-observer concordance.
Methods
We retrospectively reviewed data of 207 consecutive patients who underwent PN between January 2012 and August 2018 at a tertiary referral center. We obtained SPARE, R.E.N.A.L., and PADUA scores and evaluated correlations among the nephrometries and surgical outcomes including pentafecta by Spearman test. Logistic regression analysis was used to identify independent predictors of pentafecta outcomes. We compared the nephrometries to determine the predictive ability of achieving pentafecta using receiver operating characteristic curve analysis. Fleiss’ generalized kappa was used to assessed interobserver variation in the SPARE system.
Results
Based on the SPARE system, 120, 74, and 13 patients were stratified into low-risk, intermediate-risk, and high-risk groups, respectively. Regarding the individual components of pentafecta, there were significant differences in the complication rate (
p
= 0.03), ischemia time (
p
< 0.001), and percent change of eGFR (
p
< 0.001) among the three risk groups. In addition, higher tumor complexity was significantly associated with a lower achievement rate of pentafecta (
p
= 0.01). In Spearman correlation tests, SPARE nephrometry was correlated with ischemia time (ρ:0.37,
p
< 0.001), operative time (ρ:0.28,
p
< 0.001), complication rate (ρ:0.34,
p
< 0.001), percent change of eGFR (ρ:0.34,
p
< 0.001), and progression of chronic kidney disease stage (ρ:0.17,
p
= 0.02). Multivariate analysis revealed that SPARE significantly affected pentafecta (OR: 0.67,
p
< 0.001). In ROC curve analysis, SPARE showed fair predictive ability in the achievement pentafecta (AUC: 0.71). The predictive ability of pentafecta was similar between nephrometries (SPARE vs. R.E.N.A.L.,
p
= 0.78; SPARE vs. PADUA,
p
= 0.66). The interobserver concordance of SPARE was excellent (Kappa: 0.82,
p
= 0.03).
Conclusions
SPARE system was a predictive factor of surgical outcomes after PN. This refined nephrometry had similar predictive abilities for pentafecta achievement compared with R.E.N.A.L. and PADUA.
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