The lack of an observed supernova associated with GRB 060614 appears to require a new paradigm for the formation of (a subset of) long-duration GRBs. This requirement is based on the presumed low ...redshift of the burst, which was inferred from the spatial coincidence of the afterglow with a z = 0.125 galaxy. We explore the possibility that this low-redshift galaxy is a chance superposition along the line of sight to GRB 060614. We examine the galaxy distribution of the field of GRB 060614 and find that the probability of a chance association with a galaxy at least as bright as the putative host is only 60.5%-1.9%. However, for the current ensemble of -180 Swift GRBs, it is likely that several such coincidences have occurred, and given the "nonstandard" nature of GRB 060614 it is not implausible that this is one such occurrence. Thus, the conclusion that GRB 060614 requires a revision to the formation paradigm for long-duration GRBs should be approached with caution.
We present comprehensive multiwavelength observations of three gamma-ray bursts (GRBs) with durations of several thousand seconds. We demonstrate that these events are extragalactic transients; in ...particular, we resolve the long-standing conundrum of the distance of GRB 101225A (the "Christmas-day burst"), finding it to have a redshift z = 0.847 and showing that two apparently similar events (GRB 111209A and GRB 121027A) lie at z = 0.677 and z = 1.773, respectively. The systems show extremely unusual X-ray and optical light curves, very different from classical GRBs, with long-lasting, highly variable X-ray emission and optical light curves that exhibit little correlation with the behavior seen in the X-ray. Their host galaxies are faint, compact, and highly star-forming dwarf galaxies, typical of "blue compact galaxies." We propose that these bursts are the prototypes of a hitherto largely unrecognized population of ultra-long GRBs, which while observationally difficult to detect may be astrophysically relatively common. The long durations may naturally be explained by the engine-driven explosions of stars of much larger radii than normally considered for GRB progenitors, which are thought to have compact Wolf-Rayet progenitor stars. However, we cannot unambiguously identify supernova signatures within their light curves or spectra. We also consider the alternative possibility that they arise from the tidal disruption of stars by massive black holes and conclude that the associated timescales are only consistent with the disruption of compact stars (e.g., white dwarfs) by black holes of relatively low mass (<10 super(5) M sub(odot)).
Context. Multiwavelength observations of gamma-ray burst prompt and afterglow emission are a key tool to separate the various possible emission processes and scenarios proposed to interpret the ...complex gamma-ray burst phenomenology. Aims. We collected a large dataset on GRB 060908 in order to carry out a comprehensive analysis of the prompt emission as well as the early and late afterglow. Methods. Data from Swift-BAT, -XRT and -UVOT together with data from a number of different ground-based optical/near-infrared and millimeter telescopes allowed us to follow the afterglow evolution after about a minute from the high-energy event down to the host galaxy limit. We discuss the physical parameters required to model these emissions. Results. The prompt emission of GRB 060908 was characterised by two main periods of activity, spaced by a few seconds of low intensity, with a tight correlation between activity and spectral hardness. Observations of the afterglow began less than one minute after the high-energy event, when it was already in a decaying phase, and it was characterised by a rather flat optical/near-infrared spectrum which can be interpreted as due to a hard energy-distribution of the emitting electrons. On the other hand, the X-ray spectrum of the afterglow could be fit by a rather soft electron distribution. Conclusions. GRB 060908 is a good example of a gamma-ray burst with a rich multi-wavelength set of observations. The availability of this dataset, built thanks to the joint efforts of many different teams, allowed us to carry out stringent tests for various interpretative scenarios, showing that a satisfactorily modelling of this event is challenging. In the future, similar efforts will enable us to obtain optical/near-infrared coverage comparable in quality and quantity to the X-ray data for more events, therefore opening new avenues to progress gamma-ray burst research.
Context.GRB 050730 is a long duration high-redshift burst ($z=3.967$) that was discovered by Swift. The afterglow shows variability and was well monitored over a wide wavelength range. We present ...comprehensive temporal and spectral analysis of the afterglow of GRB 050730 including observations covering the wavelength range from the millimeter to X-rays. Aims.We use multi-wavelength afterglow data to understand the complex temporal and spectral decay properties of this high redshift burst. Methods.Five telescopes were used to study the decaying afterglow of GRB 050730 in the $B, V, r', R, i', I, J$ and K photometric pass bands. A spectral energy distribution was constructed at 2.9 h post-burst in the $B, V, R, I, J$ and K bands. X-ray data from the satellites Swift and XMM-Newton were used to study the afterglow evolution at higher energies. Results.The early afterglow shows variability at early times and the slope steepens at 0.1 days (8.6 ks) in the $B, V, r', R, i', I, J$ and K passbands. The early afterglow light curve decayed with a powerlaw slope index $\alpha_1 = -0.60\pm0.07$ and subsequently steepened to $\alpha_2 = -1.71\pm0.06$ based on the R and I band data. A millimeter detection of the afterglow around 3 days after the burst shows an excess in comparison to theoretical predictions. The early X-ray light curve observed by Swift is complex and contains flares. At late times the X-ray light curve can be fit by a powerlaw decay with $\alpha_x = -2.5\pm0.15$ which is steeper than the optical light curve. A spectral energy distribution (SED) was constructed at ~2.9 h after the burst. An electron energy index, p, of ~2.3 was calculated using the SED and the photon index from the X-ray afterglow spectra and implies that the synchrotron cooling frequency $\nu_{\rm c}$ is above the X-ray band.
WNK (with no lysine K) kinases are serine-threonine protein kinases with an atypical placement of the catalytic lysine. Intronic deletions increase the expression of WNK1 in humans and cause ...pseudohypoaldosteronism type II, a form of hypertension. WNKs have been linked to ion carriers, but the underlying regulatory mechanisms are unknown. Here, we report a mechanism for the control of ion permeability by WNK1. We show that WNK1 activates the serum- and glucocorticoid-inducible protein kinase SGK1, leading to activation of the epithelial sodium channel. Increased channel activity induced by WNK1 depends on SGK1 and the E3 ubiquitin ligase Nedd4-2. This finding provides compelling evidence that this molecular mechanism contributes to the pathogenesis of hypertension in pseudohypoaldosteronism type II caused by WNK1 and, possibly, in other forms of hypertension.
The structures of the MAP kinase p38 in complex with docking site peptides containing a φ
A-X-φ
B motif, derived from substrate MEF2A and activating enzyme MKK3b, have been solved. The peptides bind ...to the same site in the C-terminal domain of the kinase, which is both outside the active site and distinct from the “CD” domain previously implicated in docking site interactions. Mutational analysis on the interaction of p38 with the docking sites supports the crystallographic models and has uncovered two novel residues on the docking groove that are critical for binding. The two peptides induce similar large conformational changes local to the peptide binding groove. The peptides also induce unexpected and different conformational changes in the active site, as well as structural disorder in the phosphorylation lip.
We have cloned and characterized a novel mammalian serine/threonine protein kinase WNK1 (withno lysine (K)) from a rat brain cDNA library. WNK1 has 2126 amino acids and can be detected as a protein ...of ∼230 kDa in various cell lines and rat tissues. WNK1 contains a small N-terminal domain followed by the kinase domain and a long C-terminal tail. The WNK1 kinase domain has the greatest similarity to the MEKK protein kinase family. However, overexpression of WNK1 in HEK293 cells exerts no detectable effect on the activity of known, co-transfected mitogen-activated protein kinases, suggesting that it belongs to a distinct pathway. WNK1 phosphorylates the exogenous substrate myelin basic protein as well as itself mostly on serine residues, confirming that it is a serine/threonine protein kinase. The demonstration of activity was striking because WNK1, and its homologs in other organisms lack the invariant catalytic lysine in subdomain II of protein kinases that is crucial for binding to ATP. A model of WNK1 using the structure of cAMP-dependent protein kinase suggests that lysine 233 in kinase subdomain I may provide this function. Mutation of this lysine residue to methionine eliminates WNK1 activity, consistent with the conclusion that it is required for catalysis. This distinct organization of catalytic residues indicates that WNK1 belongs to a novel family of serine/threonine protein kinases.
WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other
protein kinases. These enzymes have been linked to a genetically defined ...form of hypertension. In this study we introduced
mutations to test hypotheses about the position of the catalytic lysine, and we examined mechanisms involved in the regulation
of WNK1 activity. Through the analysis of enzyme fragments and sequence alignments, we have identified an autoinhibitory domain
of WNK1. This isolated domain, conserved in all four WNKs, suppressed the activity of the WNK1 kinase domain. Mutation of
two key residues in this autoinhibitory domain attenuated its ability to inhibit WNK kinase activity. Consistent with these
results, the same mutations in a WNK1 fragment that contain the autoinhibitory domain increased its kinase activity. We also
found that WNK1 expressed in bacteria is autophosphorylated; autophosphorylation on serine 382 in the activation loop is required
for its activity.
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