Secreted protein, acidic and rich in cysteine （SPARC） is expressed in numerous types of tumors and is suggested to have prognostic value. Moreover, because of its strong affinity for albumin, and ...hence albumin-bound drugs, SPARC has increasingly become a focus for research. In this study, we aimed to determine SPARC expression in patients with non-small cell lung cancer （NSCLC） and investigate the association of SPARC with disease prognosis. Tissue microarrays were constructed with specimens from 105 patients with NSCLC treated at Sun Yat-sen University Cancer Center, and immunohistochemical analysis was performed on these tissue microarrays to assess SPARC expression. Our results showed that SPARC expression status did not significantly relate with age, gender, and tumor stage. However, SPARC was expressed more frequently in squamous cell carcinoma than in adenocarcinoma （75% vs. 43.5%, P = 0.004）. Patients with smoking history had higher SPARC expression than non-smokers （68.2% vs. 33.3%, P = 0.002）. In both univariate and multivariate analyses, SPARC was a prognostic factor of overall survival （HR = 0.32; 95% CI： 0.16-0.65） but not disease-free survival. Our study indicates that SPARC expression is higher in squamous cell carcinoma than in adenocarcinoma in NSCLC. Most notably, SPARC can be used as a prognostic factor for NSCLC.
High dielectric-permittivity (Ca0.5Cu0.5)TiO3-based ceramics have been prepared by a sol-gel method combined with a solid state sintering process. The results indicate that the additives of H3BO3 ...have a remarkable effect on the sintering temperature, microstructure and dielectric properties. High density (Ca0.5Cu0.5)TiO3 bulk ceramics can be obtained after sintering at 900°C. The as-sintered ceramics show high dielectric constants (~1000), and low losses (~0.05). The dielectric properties are nearly independent of frequency and temperature in a wide range. The activation energy is calculated as about 0.50 eV by impedance spectrum method.
Inhibition of VEGFR-2 signaling pathway is one of the most promising approaches for the treatment of cancer. In this paper, we reported the design, synthesis, and biological evaluation of a series of ...biphenylurea derivatives as VEGFR-2 inhibitors. Among these compounds, 39 exhibited potent inhibitory activity against VEGFR-2 both in vitro and in vivo. The antiangiogenesis activity of 39 was further confirmed by both tube formation assay and chick chorioallantoic membrane assay.
Al-Mg-Si-Cu alloys with and without Zn addition were fabricated by conventional ingot metallurgy method. The microstructures and properties were investigated using optical microscopy （OM）, field ...emission scanning electron microscopy （FE-SEM）, transmission electron microscopy （TEM）, tensile test, hardness test, and electrical conductivity measurement. It is found that the as-cast A1-Mg-Si-Cu-Zn alloy is composed of coarse dendritic grains, long needle-like ~/5-A1FeSi white intermetallics, and Chinese script-like a-A1FeSi compounds. During high temperature homogenization treatment, only harmful needle-like ~-A1FeSi phase undergoes fragmentation and spheroidizing at its tips, and the destruc- tive needle-like 5-phase does not show any morphological and size changes. Phase transitions from ~-A1FeSi to ~-A1FeSi and from 6-A1FeSi to 3-A1FeSi are also not found. Zn addition improves the aging hardening response during the former aging stage and postpones the peak-aged hardness to a long aging time. In T4 condition, Zn addition does not obviously increase the yield strength and decrease the elongation, but it markedly improves paint-bake hardening response during paint-bake cycle. The addition of 0.5wt% Zn can lead to an increment of 99 MPa in yield strength compared with the value of 69 MPa for the alloy without Zn after paint-bake cycle.
A protein with the activity of phospholipase A(2) named asAPLA(2) was purified to homogeneity from the venom of Agkistrodon shedaoensis Zhao through DEAE-Sepharose CL-6B anion exchange column, Source ...S, and Mono Q FPLC. Its molecular weight was estimated to be 19 kD by SDS-PAGE, and its pI was about 3.5 by IEF analysis. It inhibited the platelet aggregation that was induced by 1 micromol/ L ADP, and the IC(50) was determined to be 6 micromol/L. Degenerating primer was designed and synthesized according to the N-terminal amino acid sequence of asAPLA(2). Its full-length cDNA was cloned by RT-PCR from the total RNA extracted from the snake venom gland. Its molecular weight and the pI are determined to be 13,649 and 4.39 respectively as calculated by DNAclub and DNAstar software according to the deduced amino acid sequence. Then the gene was cloned into the expression plasmid pET-40b(+) and expressed in E. coli BL21(DE3). Western blot analysis indicated that the expressed protein cross-reacted with the antibody against the native enzyme.
High dielectric-permittivity ( Ca
0.5
Cu
0.5
)TiO
3
-based ceramics have been prepared by a sol-gel method combined with a solid state sintering process. The results indicate that the additives of H
...3
BO
3
have a remarkable effect on the sintering temperature, microstructure and dielectric properties. High density ( Ca
0.5
Cu
0.5
)TiO
3
bulk ceramics can be obtained after sintering at 900°C. The as-sintered ceramics show high dielectric constants (~1000), and low losses (~0.05). The dielectric properties are nearly independent of frequency and temperature in a wide range. The activation energy is calculated as about 0.50 eV by impedance spectrum method.
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