Three decades of authoritarian rule in Indonesia came to a sudden end in 1998. The collapse of the Soeharto regime was accompanied by massive economic decline, widespread rioting, communal conflict, ...and fears that the nation was approaching the brink of disintegration. Although the fall of Soeharto opened the way towards democratization, conditions were by no means propitious for political reform. This book asks how political reform could proceed despite such unpromising circumstances. It examines electoral and constitutional reform, the decentralization of a highly centralized regime, the gradual but incomplete withdrawal of the military from its deep political involvement, the launching of an anti-corruption campaign, and the achievement of peace in two provinces that had been devastated by communal violence and regional rebellion.
In the last few decades, Förster resonance energy transfer (FRET) based spectroscopy rulers have served as a key tool for the understanding of chemical and biochemical processes, even at the single ...molecule level. Since the FRET process originates from dipole-dipole interactions, the length scale of a FRET ruler is limited to a maximum of 10 nm. Recently, scientists have reported a nanomaterial based long-range optical ruler, where one can overcome the FRET optical ruler distance dependence limit, and which can be very useful for monitoring biological processes that occur across a greater distance than the 10 nm scale. Advancement of nanoscopic long range optical rulers in the last ten years indicate that, in addition to their long-range capability, their brightness, long lifetime, lack of blinking, and chemical stability make nanoparticle based rulers a good choice for long range optical probes. The current review discusses the basic concepts and unique light-focusing properties of plasmonic nanoparticles which are useful in the development of long range one dimensional to three dimensional optical rulers. In addition, to provide the readers with an overview of the exciting opportunities within this field, this review discusses the applications of long range rulers for monitoring biological and chemical processes. At the end, we conclude by speculating on the role of long range optical rulers in future scientific research and discuss possible problems, outlooks and future needs in the use of optical rulers for technological applications.
This review reports recent advances in the development of long-range nanomaterial-based optical rulers.
Hepatocellular carcinoma (HCC) is the 2nd leading cause of cancer-related deaths every year globally. The most common form of treatment, hepatic arterial infusion (HAI), involves the direct injection ...of doxorubicin (DOX) into the hepatic artery. It is plagued with limited therapeutic efficacy and the occurrence of severe toxicities (e.g. cardiotoxicity). We aim to improve the therapeutic index of DOX delivered via HAI by loading the drug onto generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers targeted to hepatic cancer cells via N-acetylgalactosamine (NAcGal) ligands. DOX is attached to the surface of G5 molecules via two different enzyme-sensitive linkages, L3 or L4, to achieve controllable drug release inside hepatic cancer cells. We previously reported on P1 and P2 particles that resulted from the combination of NAcGal-targeting with L3- or L4-DOX linkages, respectively, and showed controllable DOX release and toxicity towards hepatic cancer cells comparable to free DOX. In this study, we demonstrate that while the intratumoral delivery of free DOX (1 mg/kg) into HCC-bearing nod scid gamma (NSG) mice achieves a 2.5-fold inhibition of tumor growth compared to the saline group over 30 days, P1 and P2 particles delivered at the same DOX dosage achieve a 5.1- and 4.4-fold inhibition, respectively. Incubation of the particles with human induced pluripotent stem cell derived cardiomyocytes (hiPSC CMs) showed no effect on monolayer viability, apoptosis induction, or CM electrophysiology, contrary to the effect of free DOX. Moreover, magnetic resonance imaging revealed that P1- and P2-treated mice maintained cardiac function after intraperitoneal administration of DOX at 1 mg/kg for 21 days, unlike the free DOX group at an equivalent dosage, confirming that P1/P2 can avoid DOX-induced cardiotoxicity. Taken together, these results highlight the ability of P1/P2 particles to improve the therapeutic index of DOX and offer a replacement therapy for clinical HCC treatment.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abstract
Marine bivalves are important components of ecosystems and exploited by humans for food across the world, but the intrinsic vulnerability of exploited bivalve species to global changes is ...poorly known. Here, we expand the list of shallow-marine bivalves known to be exploited worldwide, with 720 exploited bivalve species added beyond the 81 in the United Nations FAO Production Database, and investigate their diversity, distribution and extinction vulnerability using a metric based on ecological traits and evolutionary history. The added species shift the richness hotspot of exploited species from the northeast Atlantic to the west Pacific, with 55% of bivalve families being exploited, concentrated mostly in two major clades but all major body plans. We find that exploited species tend to be larger in size, occur in shallower waters, and have larger geographic and thermal ranges—the last two traits are known to confer extinction-resistance in marine bivalves. However, exploited bivalve species in certain regions such as the tropical east Atlantic and the temperate northeast and southeast Pacific, are among those with high intrinsic vulnerability and are a large fraction of regional faunal diversity. Our results pinpoint regional faunas and specific taxa of likely concern for management and conservation.
This anthology brings together the late Barry A. Crouch’s most important articles on the African American experience in Texas during Reconstruction. Grouped topically, the essays explore what freedom ...meant to the newly emancipated, how white Texans reacted to the freed slaves, and how Freedmen’s Bureau agents and African American politicians worked to improve the lot of ordinary African American Texans. The volume also contains Crouch’s seminal review of Reconstruction historiography, “Unmanacling Texas Reconstruction: A Twenty-Year Perspective.” The introductory pieces by Arnoldo De Leon and Larry Madaras recapitulate Barry Crouch’s scholarly career and pay tribute to his stature in the field of Reconstruction history.
Identifying relationships between variables in ecological systems is challenging due to the large number of interacting factors. One system studied in detail is avian reproduction, where molecular ...analyses have revealed dramatic variation in rates of extra-pair paternity-the frequency with which broods contain individuals sired by different males. Despite the attention the topic has received, identification of ecological predictors of the observed variation remains elusive. In this study we evaluate how structural equation modeling-which allows for simultaneous estimation of covariation between all variables in a model-can help identify significant relationships between ecological variables and extra-pair paternity. We estimated the correlation of eight different variables using data from 36 species of passerines by including them in six different models of varying complexity. We recover strong support for species with lower rates of male care having higher rates of extra-pair paternity. Our results also suggest that testes size, range size, and longevity all potentially have a relationship with rates of extra-pair paternity; however, interpretation of this result is more challenging. More generally, these results demonstrate the utility of applying structural equation modeling to understanding correlations among interacting variables in complex biological systems.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
In this highly respected work, Harold Crouch analyzes the role of the Indonesian army in that country's politics, putting special emphasis on the Sukarno years, the gradual take-over of power by the ...military, and the nature of Suharto's New Order government. The Army and Politics in Indonesia is now available in a paperback edition, updated with a new preface and epilogue that expand the book's coverage to the 1980s.
The objective testing of algorithms for performing ambiguity resolution in vector magnetic field data is continued, with an examination of the effects of noise in the data. Through the use of ...analytic magnetic field models, two types of noise are “added” prior to resolving: noise to simulate Poisson photon noise in the observed polarization spectra, and a spatial binning to simulate the effects of unresolved structure. The results are compared through the use of quantitative metrics and performance maps. We find that while no algorithm severely propagates the effects of Poisson noise beyond very local influences, some algorithms are more robust against high photon-noise levels than others. In the case of limited spatial resolution, loss of information regarding fine-scale structure can easily result in erroneous solutions. Our tests imply that photon noise and limited spatial resolution can act so as to make assumptions used in some ambiguity resolution algorithms no longer consistent with the observed magnetogram. We confirm a finding of the earlier comparison study that results can be very sensitive to the details of the treatment of the observed boundary and the assumptions governing that treatment. We discuss the implications of these findings, given the relative sensitivities of the algorithms to the two sources of noise tested here. We also touch on further implications for interpreting observational vector magnetic field data for general solar physics research.
We continue the investigation of how to use the divergence-free condition to resolve the azimuthal ambiguity present in vector magnetogram data. In previous articles by Crouch, Barnes, and Leka (
...Solar Phys.
260
, 271,
2009
) and Crouch (
Solar Phys.
282
, 107,
2013
), all methods used an expression for the divergence of the magnetic field that involves differentiation of quantities that depend on the choice of azimuthal angle. As a result, all heights used to approximate line-of-sight derivatives should generally be disambiguated simultaneously. In this article, we investigate a set of methods that use an expression for the divergence that involves differentiation of quantities that do not depend on the choice of azimuthal angle. This results in an expression for the divergence that can be used to disambiguate each height independently. We test two methods using synthetic data and find that the two-step hybrid method, adapted to disambiguate each height independently, generally produces reasonable results. Moreover, the time required to compute solutions is substantially decreased in comparison to the corresponding method that disambiguates all relevant heights simultaneously.
In this comprehensive account, Harold Crouch argues that, while they may appear contradictory, the responsive and the repressive features of the system combine in an integrated and coherent whole.
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