While interplay between BRCA1 and AURKA-RHAMM-TPX2-TUBG1 regulates mammary epithelial polarization, common genetic variation in HMMR (gene product RHAMM) may be associated with risk of breast cancer ...in BRCA1 mutation carriers. Following on these observations, we further assessed the link between the AURKA-HMMR-TPX2-TUBG1 functional module and risk of breast cancer in BRCA1 or BRCA2 mutation carriers. Forty-one single nucleotide polymorphisms (SNPs) were genotyped in 15, 252 BRCA1 and 8, 211 BRCA2 mutation carriers and subsequently analyzed using a retrospective likelihood approach. The association of HMMR rs299290 with breast cancer risk in BRCA1 mutation carriers was confirmed: per-allele hazard ratio (HR) = 1.10, 95% confidence interval (CI) 1.04 - 1.15, p = 1.9 x 10(-4) (false discovery rate (FDR)-adjusted p = 0.043). Variation in CSTF1, located next to AURKA, was also found to be associated with breast cancer risk in BRCA2 mutation carriers: rs2426618 per-allele HR = 1.10, 95% CI 1.03 - 1.16, p = 0.005 (FDR-adjusted p = 0.045). Assessment of pairwise interactions provided suggestions (FDR-adjusted p(interaction) values > 0.05) for deviations from the multiplicative model for rs299290 and CSTF1 rs6064391, and rs299290 and TUBG1 rs11649877 in both BRCA1 and BRCA2 mutation carriers. Following these suggestions, the expression of HMMR and AURKA or TUBG1 in sporadic breast tumors was found to potentially interact, influencing patients' survival. Together, the results of this study support the hypothesis of a causative link between altered function of AURKA-HMMR-TPX2-TUBG1 and breast carcinogenesis in BRCA1/2 mutation carriers.
Many antimicrobial compounds in mouthwashes can have a negative impact on the oral microbiome. O-cymen-5-ol, a compound derived from a phytochemical, has a targeted mode of action and is being used ...as an alternative. However, its effect on the native oral microbiome is unknown.
To assess the effect of a mouthwash formulated with o-cymen-5-ol and zinc chloride on the oral microbiome of healthy individuals.
A mouthwash formulated with o-cymen-5-ol and zinc chloride was administered to a cohort of 51 volunteers for 14 days, while another cohort of 49 volunteers received a placebo. The evolution of the oral microbiome in both groups was analysed using a metataxonomic approach.
Analysis of the oral microbiome showed that the mouthwash selectively targeted potential oral pathogens while maintaining the integrity of the rest of the microbiome. Specifically, the relative abundance of several potentially pathogenic bacterial taxa, namely Fusobacteriota, Prevotella, Actinomyces, Granulicatella, Abiotrophia, Lautropia, Lachnoanaerobaculum, Eubacterium (nodatum group) and Absconditabacteriales (SR1) decreased, while the growth of Rothia, a nitrate-reducing bacterium beneficial for blood pressure, was stimulated.
The use of o-cymen-5-ol and zinc chloride as antimicrobial agents in oral mouthwashes is a valuable alternative to classical antimicrobial agents.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
The development of psychomotor skills allows students to demonstrate movement skills, knowledge and social relationships that strengthen the personality of the individual. The purpose of the study ...was to propose a plan of socio-educational strategies for the development of psychomotor skills in early education students of the Sewarare 1200 kindergarten, located in Mexico. It was configured within a quantitative paradigm, of a descriptive-projective type, with a transversal non-experimental design, with a sample of 47 students and 4 teachers, to whom a questionnaire structured in two parts was applied. The results showed that initial education teachers in the area of Physical Education have moderate and low knowledge in the management of socio-educational strategies. While the students demonstrated a moderate and low level of psychomotor competencies. Hence the need to propose a plan of socio-educational strategies for the development of psychomotor skills in students which offers elements of experiences, adaptation to space, relationship with others, bringing rewarding and healthy emotions, essential for the future performance of the learner in the different facets of life, besides being a pedagogical tool to help teachers to contribute and improve their school practice.
El desarrollo de competencias psicomotoras permite que los estudiantes demuestren habilidades de movimientos, conocimientos y relaciones sociales que fortalecen la personalidad del individuo. El propósito del estudio fue proponer un Plan de estrategias socioeducativas para el desarrollo de competencias psicomotoras en estudiantes de educación inicial del Jardín Sewarare 1200, ubicado en México. Se configuró dentro de un paradigma cuantitativo, de tipo descriptivo-proyectivo, con un diseño no experimental transversal, con una muestra de 47 estudiantes y 4 docentes, a quienes se les aplicó un cuestionario estructurado en dos partes. Los resultados demostraron que los docentes de educación inicial en el área de Educación Física poseen conocimientos moderado y bajo en el manejo de estrategias socioeducativas. Mientras que los estudiantes demostraron un moderado y bajo nivel de competencias psicomotoras. De allí la necesidad de proponer un plan de estrategias socioeducativas para el desarrollo de competencias psicomotoras en los estudiantes la cual ofrece elementos de vivencias, adaptación al espacio, relación con otros, trayendo emociones gratificantes y saludables, esenciales para el futuro desempeño del educando en las diferentes facetas de la vida, además de ser una herramienta de ayuda pedagógica a los docentes para contribuir y mejorar su práctica escolar.
Chlorhexidine (CHX) is one of the most widely used antiseptics in the oral cavity due to its high antimicrobial potential. However, many authors have stated that the effect of CHX in nonsurgical ...periodontal therapy is hampered by its rapid elimination from the oral environment. The aim of this study was to determine the antibacterial efficacy of a new compound of chlorhexidine 0.20% + cymenol (CYM) 0.10% on a multispecies biofilm. For this, an in vitro study was designed using a multispecies biofilm model of
,
,
, and
. Quantification of the microbial viability of the biofilm was performed using 5-cyano-2,3-ditolyl tetrazolium-chloride (CTC) to calculate the percentage of survival, and the biofilms were observed using a a confocal laser scanning microscopy (CLSM). It was observed that the bactericidal activity of the CHX + cymenol bioadhesive gel was superior to that of the CHX bioadhesive gel, in addition to higher penetrability into the biofilm. Therefore, there was greater elimination of bacterial biofilm with the new compound of chlorhexidine 0.2% plus cymenol 0.1% in a bioadhesive gel form compared to the formulation with only chlorhexidine 0.2% in a bioadhesive gel form.
To describe the primary cancer sites and clinical features of choroidal metastasis in Mexican patients.
This was a retrospective, observational, and multi-center study. Data were recollected from 6 ...ophthalmological hospitals in Mexico from patients with choroidal metastasis diagnosed from 2000 to 2018.
Seventy-eight patients were studied: 43 were female and 35 were male. Mean age at presentation was 57.6 years. Overall, primary cancer sites were: 1) breast: 27 cases (34.6%); 2) lung: 19 cases (24.3%); 3) unknown: 8 cases (10.2%); 4) gastrointestinal: 7 cases (8.9%); 5) renal: 5 cases (6.4%); 6) testicular: 3 cases (3.8%); 7) ovary: 3 Cases (3.8%); 8) prostate: 2 cases (2.5%); 9) thyroid: 2 cases (2.5%); 10) carcinoid: 1 case (1.2%); and 11) multiple myeloma: 1 case (1.2%). Divided by gender, for women, the main three sites were: breast, unknown, and ovary. For men, the main three sites were: lung, gastrointestinal, and testicular. Oldest cases were breast cancer (87 and 85 years); youngest cases were testicular (23 and 25 years). Solitary lesions were observed in 56 cases (71.7%); multiple lesions were observed in 22 cases (28.2%). Forty-two cases had a white or yellowish color, while 6 cases presented an orange color.
Primary cancer sites and clinical features of choroidal metastasis in Mexican patients show important differences from other populations previously studied, mainly the presence of a higher proportion of gastrointestinal and renal cancer, as well as higher incidence of ovarian and testicular cancer. These types of cancer, although not as common as breast or lung, need to be taken into account when studying Mexican patients living abroad.
The development of psychomotor skills allows students to demonstrate movement skills, knowledge and social relationships that strengthen the personality of the individual. The purpose of the study ...was to propose a plan of socio-educational strategies for the development of psychomotor skills in early education students of the Sewarare 1200 kindergarten, located in Mexico. It was configured within a quantitative paradigm, of a descriptive-projective type, with a transversal non-experimental design, with a sample of 47 students and 4 teachers, to whom a questionnaire structured in two parts was applied. The results showed that initial education teachers in the area of Physical Education have moderate and low knowledge in the management of socio-educational strategies. While the students demonstrated a moderate and low level of psychomotor competencies. Hence the need to propose a plan of socio-educational strategies for the development of psychomotor skills in students which offers elements of experiences, adaptation to space, relationship with others, bringing rewarding and healthy emotions, essential for the future performance of the learner in the different facets of life, besides being a pedagogical tool to help teachers to contribute and improve their school practice. Key words: Learning, didactic activities, sensory coordination, physical education, movements.
Abstract only
TPS209
Background: Multiple lines of evidence suggest a crosstalk between androgen receptor (AR) signaling and DNA damage repair (DDR) in prostate cancer. Co-targeting both pathways in ...mHNPC can result in a clinically relevant synergistic effect. ZZFIRST trial is evaluating the combination of TALA –a poly(ADP-ribose) polymerase inhibitor– and EZ –an AR signaling inhibitor– in mHNPC patients. Methods: This is a multicenter, open-label, randomized, investigator-initiated phase 2 clinical trial. Men aged ≥18 years with histologically confirmed mHNPC, an ECOG performance status of 0-1, and a prostate-specific antigen (PSA) ≥4 ng/mL at enrolment are eligible. Patients must have not received previous systemic treatment for locally advanced or mHNPC. A total of 54 patients will start treatment with EZ 160 mg/day for 2 28-day cycles in addition to standard androgen-deprivation therapy (ADT). Patients are then randomized and stratified based on homologous recombination gene alterations on a 1:2 ratio to either continue EZ 160 mg/day, or to receive EZ 160 mg/day plus TALA 0.5 mg/day. In both arms, patients will continue ADT throughout the trial. Treatment will continue until progressive disease (PD) or unacceptable toxicity. PSA will be determined every 4 weeks and radiological tumor extend will be assessed at screening and every 8 weeks for the 6 initial months of treatment and every 12 weeks thereafter until PD. Primary endpoint is PSA-complete response defined as the percentage of patients with PSA < 0.2 ng/mL at 12 months of therapy. Secondary endpoints include PSA-complete response at any time point and at month 7, PSA response ( < 4 ng/ml) at 7 and 12 months, PSA-progression-free survival (PSA-PFS), radiologic PFS, time to castration resistance based on PSA-PFS and rPFS, and overall survival. Safety will be assessed as per NCI-CTCAE 5.0. Exploratory endpoints include analysis of transcriptional changes in AR and DDR pathways and assessment of genomic signatures on tumor and liquid biopsies collected at baseline, 4 weeks, and PD. An imaging sub-study of whole-body diffusion weighted MRI will help to further study antitumor activity and drug resistance mechanisms. This trial was opened to accrual in July 2020. Currently 44 patients have been enrolled (with 37 randomized by cycle 3 day 1) out of 54 expected. Analysis will be assessed with the exact binomial test. At least 11 patients must maintain PSA < 0.2 ng/mL by 12 months of therapy among 32 evaluable patients in the combination arm to justify further investigation of this strategy. A drop-out rate of 10% has been considered. Clinical trial information: NCT04332744.
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