The Profile of Subjective Life-Quality questionnaire is presented, describing the conceptual foundations of its design. The questionnaire was developed to specifically evaluate life quality & changes ...in life quality of patients marked by psychiatric or somatic pathology. The questionnaire's measures include performance, satisfaction, & perceived achievement of life goals, using such variables as functioning in various areas (eg, motor, intellectual), relationships, emotional/spiritual life, socioeconomic status, & mood. The questionnaire was tested & validated in a sample of 1,500 pathological Ss. 5 Figures, 21 References. Adapted from the source document.
The number of ACTH-(1-24) receptors and the adenylate cyclase (AC) responsiveness to several stimuli have been measured in crude adrenal membranes from fetal and newborn lambs. At 124 days of ...gestation, the number of ACTH-(1-24)-binding sites was 51 +/- 9 (SE) pmol/microgram DNA, and AC was stimulated by 2 x 10(-2) M NaF (195 +/- 5%) and 10(-5) M guanosine (beta, gamma-imido) triphosphate Gpp(NH)p; (168 +/- 8%) but not by 10(-6) M ACTH-(1-24). However, when Gpp(NH)p was added to ACTH-(1-24), the response was significantly higher than with Gpp(NH)p alone. On the contrary, at 140 days, despite no increase in the number of ACTH-(1-24) receptors, AC was stimulated by ACTH-(1-24) (135 +/- 6%), and Gpp(NH)p enhanced this response. In addition, the response to NaF and to Gpp(NH)p alone was higher at 140 than at 124 days. Between day 140 of gestation and birth, the stimulation of AC by ACTH-(1-24) and ACTH-(1-24) plus Gpp(NH)p increased, but the response to NaF and to Gpp(NH)p alone did not. During the same time, the number of ACTH-(1-24) receptors was increased by a factor of 3. No change in the Kd and Km of ACTH-(1-24) was observed during the period studied. Neither prostaglandin E2 (10(-5) M) nor alpha MSH (10(-6) M) stimulated AC at any stage. The relative insensitivity of AC before 140 days can thus be related to 1) a defect of availability of GTP, and 2) a low activity of the catalytic subunit of AC (subunit C) or, alternatively, a defect in the GTP-binding component (subunit N). The increased sensitivity to ACTH-(1-24) of the enzyme just before parturition correlates closely with an increase in the number of ACTH-(1-24) receptors.
RÉSUMÉ
Cet article présente les bases conceptuelles qui ont présidé à l'élaboration d'un questionnaire (« Profil de la Qualité de Vie Subjective » ou « PQVS ») évaluant la qualité de vie subjective ...des patients en pathologie somatique et psychiatrique. Une description de l'instrument précède les données concernant la validation de ce questionnaire ainsi que les résultats déjà obtenus sur environ 1500 sujets présentant des pathologies différentes. Les premiers résultats montrent qu'à chaque pathologie correspond un profil différent de patients; qu'il est possible, grâce à ce nouvel instrument d'évaluation, de mettre en évidence des changements spécifiques et parfois inattendus chez les patients; et qu'enfin, dans un certain nombre de cas, l'on peut dégager des hypothèses explicatives de ces changements.
The effects in vivo ACTH administration on the synthesis of mRNA coding for total adrenal proteins and for protein E a specific marker of ACTH action, have been studied. After 4 h of in vivo ACTH ...treatment, protein E is one of the major translational products. Its electrophoretic characteristics in a 2D gel acrylamide system are defined (molecular mass = 36,000 daltons, pHi = 7). We have investigated the effects of ACTH on both poly(A)-RNA coding for total adrenal proteins, and non-poly(A)-RNA. The time course of these effects is different: the effect on mRNA is maximal at 48 h whereas the effect on non-poly(A)-RNA continues to increase until the end of the experiment (5 days). In vitro translocational assays of mRNA indicate that the highest efficiency (protein synthesis/microgram of mRNA) is observed after 4 h of ACTH treatment in vivo. After 5 days this efficiency is similar to that of mRNA extracted from non ACTH-treated rats.
The mechanism of corticotropin stimulation of the synthesis of a specific rat adrenal cytosolic protein was investigated. This protein (protein E) has a mol.wt. of approx. 30000. It is detected by ...polyacrylamide-gel electrophoresis of cytosol prepared from adrenal slices from rats treated with corticotropin in vivo and control rats, the slices being incubated with (3)H- and (14)C-leucine respectively. In rats 1-15 days after hypophysectomy, corticotropin, like dibutyryl cyclic AMP, induces an increase in protein E similar to that induced in control rats, even though both compounds no longer stimulate total protein synthesis. Corticotropin stimulation of protein E synthesis is mediated by cyclic AMP but not by corticosterone, since aminoglutethimide, a steroidogenic inhibitor, does not affect corticotropin stimulation, and dexamethasone alone has no effect. Actinomycin D, when injected in vivo 1h before or after corticotropin injection, prevents the effect of corticotropin on protein E synthesis, which is interpreted as evidence that mRNA synthesis is necessary for the stimulation of protein E synthesis. When injected more than 2h after corticotropin, actinomycin D does not prevent corticotropin stimulation of protein E synthesis, but completely blocks corticotropin stimulation of total protein synthesis. This is interpreted as meaning that, after stimulation of mRNA coding for protein E, corticotropin has no effect on the synthesis of protein E. On the other hand, corticotropin stimulation of protein E synthesis persists after hypophysectomy even though it no longer stimulates total protein synthesis. These data suggest that the factor(s) involved in the synthesis of protein E are more stable than those involved in total protein synthesis.
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