Currently, mushroom poisoning still poses a huge problem to humans' health and life globally. Poisoning incidents caused by
Inosperma
spp. were reported continuously in tropical China in recent ...years. In this study, a new poisonous
Inosperma
species, discovered from a poisoning incident, was described in tropical China based on morphological, molecular, and toxin detection evidence; detailed descriptions, photographs, and comparisons to closely related species were provided. For qualitative analysis, through targeted screening using ultra-high liquid chromatography triple quadrupole mass spectrometry (UPLC-MS/MS), the new species contains muscarine and no other toxins (two isoxazole derivatives, two tryptamine alkaloids, three amatoxins, and three phallotoxins). For quantitative analysis, muscarine contents in the pileus and the stipe were 2.08 ± 0.05 and 6.53 ± 1.88 g/kg, respectively.
Mallocybe
, a member of the ectomycorrhizal mushroom family
Inocybaceae
, is a newly elevated genus based on
Inocybe
subg.
Mallocybe
. Although new species of
Mallocybe
have continuously been ...discovered worldwide during recent decades, species native to China are poorly documented. Members of the genus are frequently characterized by the presence of phaseoliform basidiospores and short, catenate cheilocystidia. Here, we describe three new
Mallocybe
species from China featuring elongate cheilocystidia that were revealed through morphological and molecular analyses:
M. pallidipes
collected from
Populus
in northeastern China,
M. aurantiodisca
discovered in subtropical evergreen broad-leaved forests dominated by
Castanopsis
in Zhejiang and Guizhou provinces, and
M. longicystis
found in fagaceous forests in tropical and subtropical China. According to a multi-gene phylogenetic analysis, which resolved the genus into three major clades, the three new species group with three North American species in a fully supported clade. Finally, we discuss the systematic position of the new species and infrageneric phylogeny of the genus.
An accurate identification of poisonous mushrooms and the confirmation of the toxins involved are both of great importance in the treatment of mushroom poisoning incidents. In recent years, cases of ...mushroom poisoning by
spp. have been repeatedly reported from tropical Asia. It is urgent to know the real species diversity of
in this region. In the present study, we proposed two new
species from tropical Asia, namely
and
. They were described based on morphology and multilocus phylogeny. Detailed descriptions, color photographs and the discussion with other closely related species of the two new taxa were provided. In addition, a comprehensive muscarine determination of these two new species using ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) approach has been performed. Results showed that these two species were muscarine positive, with a content of 16.03 ± 1.23 g/kg in
and a content of 11.87 ± 3.02 g/kg in
, much higher than the known species
. Recovery of muscarine ranged from 93.45% to 97.25%, and the average recovery is 95.56%.
Deficits in the clearance of amyloid β-protein (Aβ) play a pivotal role in the pathogenesis of sporadic Alzheimer's disease (AD). The roles of blood monocytes in the development of AD remain unclear. ...In this study, we sought to investigate the alterations in the Aβ phagocytosis function of peripheral monocytes during ageing and in AD patients. A total of 104 cognitively normal participants aged 22-89 years, 24 AD patients, 25 age- and sex-matched cognitively normal (CN) subjects, 15 Parkinson's disease patients (PD), and 15 age- and sex-matched CN subjects were recruited. The Aβ uptake by blood monocytes was measured and its alteration during ageing and in AD patients were investigated. Aβ
uptake by monocytes decreased during ageing and further decreased in AD but not in PD patients. Aβ
uptake by monocytes was associated with Aβ
levels in the blood. Among the Aβ uptake-related receptors and enzymes, the expression of Toll-like receptor 2 (TLR2) was reduced in monocytes from AD patients. Our findings suggest that monocytes regulate the blood levels of Aβ and might be involved in the development of AD. The recovery of the Aβ uptake function by blood monocytes represents a potential therapeutic strategy for AD.
Urolithin A (UroA) can inhibit the growth of many human cancer cells, but it has not be reported if UroA inhibits nasopharyngeal carcinoma (NPC) cells.
To explore the inhibitory effect of UroA on NPC ...and potential mechanism in vitro.
RNA-sequencing-based mechanistic prediction was conducted by comparing KEGG enrichment of 40 μM UroA-treated for 24 h with untreated CNE2 cells. The untreated cells were selected as control. After NPC cells were treated with 20-60 μM UroA, proliferation, migration and invasion of were measured by colony formation, wound healing and transwell experiments. Apoptosis, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) were measured by flow cytometry, Hoechst 33342, Rhodamine 123, JC-1 staining and ROS assay methods, respectively. Gene and protein expression were measured by RT-qPCR and Western blotting assay.
RNA-sequencing and KEGG enrichment revealed UroA mainly altered the ECM receptor interaction pathway. UroA inhibited cells proliferation, epithelial-mesenchymal-transition pathway, migration and invasion with IC
50
values of 34.72 μM and 44.91 μM, induced apoptosis, MMP depolarization and increase ROS content at a concentration of 40 μM. UroA up-regulated E-cadherin, Bax/Bcl-2, c-caspase-3 and PARP proteins, while inhibiting COL4A1, MMP2, MMP9, N-cadherin, Vimentin and Snail proteins at 20-60 μM. Moreover, co-treatment of UroA (40 μM) and NAC (5 mM) could reverse the effect of UroA on apoptosis-related proteins.
RNA-sequencing technology based on bioinformatic analyses may be applicable for studiying the mechanism of drugs for tumour treatment.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was ...used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads' diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found.
Traditional model tests for soil and rock materials face challenges in observing the slurry diffusion within the soil mass, hindering the understanding of the relationship between grouting-induced ...ground deformation and grout diffusion. This study conducts grouting diffusion model tests using a self-developed experimental setup on both ordinary and transparent sand. We investigate cement slurry diffusion patterns, distribution characteristics, and temporal variations in ground uplift displacement during the grouting process. By leveraging a visualization grouting model and non-intrusive displacement measurements, we directly observe and verify the changes in cement slurry diffusion and ground displacement in transparent sand. The results indicate the following: during non-steady grouting in sand, slurry diffusion progresses from low-pressure infiltration to medium-pressure compaction, culminating in high-pressure fracturing; ground uplift displacement curves exhibit a consistent “step-like” increase with grouting time, featuring accelerated growth after each step; and visualization tests reveal a strong correlation between grouting pressure, slurry diffusion, and corresponding uplift displacement. Distinct features in the grouting pressure plot align with the acceleration phases of the displacement; at a water–cement ratio (w/c) of 0.8, the stratum’s vertical deformation shows a symmetric “higher in the middle, lower on the sides” distribution. As the burial depth decreases, the stratum’s uplift displacement tends to flatten horizontally, especially at w/c = 0.8 and 1.2.
Wide-bandgap gallium oxide (Ga2O3) is one of the most promising semiconductor materials for solar-blind (200 nm–280 nm) photodetection. In its amorphous form, a-Ga2O3 maintains its intrinsic ...optoelectronic properties while can be prepared at a low growth temperature, thus it is compatible with Si integrated circuits (ICs) technology. Herein, the a-Ga2O3 film is directly deposited on pre-fabricated Au interdigital electrodes by plasma enhanced atomic layer deposition (PE-ALD) at a growth temperature of 250 °C. The stoichiometric a-Ga2O3 thin film with a low defect density is achieved owing to the mild PE-ALD condition. As a result, the fabricated Au/a-Ga2O3/Au photodetector shows a fast time response, high responsivity, and excellent wavelength selectivity for solar-blind photodetection. Furthermore, an ultra-thin MgO layer is deposited by PE-ALD to passivate the Au/a-Ga2O3/Au interface, resulting in the responsivity of 788 A/W (under 254 nm at 10 V), a 250-nm-to-400-nm rejection ratio of 9.2 × 103, and the rise time and the decay time of 32 ms and 6 ms, respectively. These results demonstrate that the a-Ga2O3 film grown by PE-ALD is a promising candidate for high-performance solar-blind photodetection and potentially can be integrated with Si ICs for commercial production.
This paper presents a new steam sterilization approach that uses a solar-driven evaporation system at the water/air interface. Compared to the conventional solar autoclave, this new steam ...sterilization approach via interfacial evaporation requires no complex system design to bear high steam pressure. In such a system, a reduced graphene oxide/polytetrafluoroethylene composite membrane floating at the water/air interface serves as a light-to-heat conversion medium to harvest and convert incident solar light into localized heat. Such localized heat raises the temperature of the membrane substantially and helps generate steam with a temperature higher than 120 °C. A sterilization device that takes advantage of the interfacial solar-driven evaporation system was built and its successful sterilization capability was demonstrated through both chemical and biological sterilization tests. The interfacial evaporation-based solar driven sterilization approach offers a potential low cost solution to meet the need for sterilization in undeveloped areas that lack electrical power but have ample solar radiation.
PDF
