Mastitis is one of the major diseases of dairy cows that affects milk quality and quantity and increases the potential risk for the presence of antimicrobial residues (AR) in milk, which could lead ...to the development of antimicrobial resistance (AMR) among human pathogens. Even if the presence of AR in milk and milk products is low in many countries, the threat is not negligible and cannot be ignored. These problems may be investigated by applying a One Health approach, and this prevalence study aimed to estimate the risks for human health related to milk production applied to dairy herds in Lombardy. Three hundred thirty-one bulk tank milk samples were randomly collected and analyzed by CombiFoss 7 and MilkoScan 7 (milk quality, bacteria, and somatic cell count), an HPLC system coupled to a Q-Exactive Orbitrap (AR), and qPCR (contagious pathogens). The data were analyzed by a generalized linear model. The results showed a relatively high prevalence of contagious pathogens (S. aureus 28.1%; Str. agalactiae 7.3%; M. bovis 3%), which primarily affect milk nutritional components decreasing mainly milk fat content (range 1%–2.5%), but did not show them to be associated to an increase of the risk of antimicrobial residues. These latter ones were recovered only in 7/331 samples at concentrations far below official MLRs. The results support currently active surveillance programs’ efficacy in reducing AR risks, which may be further improved by prioritizing them based on geographical area characteristics.
Pluto and its satellite, Charon (discovered in 1978; ref. 1), appear to form a double planet, rather than a hierarchical planet/satellite couple. Charon is about half Pluto's size and about ...one-eighth its mass. The precise radii of Pluto and Charon have remained uncertain, leading to large uncertainties on their densities. Although stellar occultations by Charon are in principle a powerful way of measuring its size, they are rare, as the satellite subtends less than 0.3 microradians (0.06 arcsec) on the sky. One occultation (in 1980) yielded a lower limit of 600 km for the satellite's radius, which was later refined to 601.5 km (ref. 4). Here we report observations from a multi-station stellar occultation by Charon, which we use to derive a radius, RC = 603.6 ± 1.4 km (1σ), and a density of ρ = 1.71 ± 0.08 g cm-3. This occultation also provides upper limits of 110 and 15 (3σ) nanobar for an atmosphere around Charon, assuming respectively a pure nitrogen or pure methane atmosphere.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The main scientific goal of TESS is to find planets smaller than Neptune around stars that are bright enough to allow for further characterization studies. Given our current instrumentation and ...detection biases, M dwarfs are prime targets in the search for small planets that are in (or near) the habitable zone of their host star. In this work, we use photometric observations and CARMENES radial velocity (RV) measurements to validate a pair of transiting planet candidates found by TESS. The data were fitted simultaneously, using a Bayesian Markov chain Monte Carlo (MCMC) procedure and taking into account the stellar variability present in the photometric and spectroscopic time series. We confirm the planetary origin of the two transiting candidates orbiting around TOI-2095 (LSPM J1902+7525). The star is a nearby M dwarf (
d
= 41.90 ± 0.03 pc,
T
eff
= 3759 ± 87 K,
V
= 12.6 mag), with a stellar mass and radius of
M
*
= 0.44 ± 0.02
M
⊙
and
R
*
= 0.44 ± 0.02
R
⊙
, respectively. The planetary system is composed of two transiting planets: TOI-2095b, with an orbital period of
P
b
= 17.66484 ± (7 × 10
−5
) days, and TOI-2095c, with
P
c
= 28.17232 ± (14 × 10
−5
) days. Both planets have similar sizes with
R
b
= 1.25 ± 0.07
R
⊕
and
R
c
= 1.33 ± 0.08
R
⊕
for planet b and planet c, respectively. Although we did not detect the induced RV variations of any planet with significance, our CARMENES data allow us to set stringent upper limits on the masses of these objects. We find
M
b
< 4.1
M
⊕
for the inner and
M
c
<
7.4
M
⊕
for the outer planet (95% confidence level). These two planets present equilibrium temperatures in the range of 300–350 K and are close to the inner edge of the habitable zone of their star.
Context. The physical characterization of trans-Neptunian objects is essential for improving our understanding of the formation and evolution of our Solar System. Stellar occultation is a ...ground-based technique that can be successfully used to determine some of the TNOs' fundamental physical properties with high precision, such as size and shape. Aims: This work is aimed at constraining the size, shape, and geometric albedo of the dwarf planet candidate (307261) 2002 MS4 through the analysis of nine stellar occultation events. Using multichord detection, we also study the object's topography by analyzing the obtained limb and residuals between the observed chords and the best-fit ellipse. Metods. We predicted and organized the observational campaigns of nine stellar occultations by 2002 MS4 between 2019 and 2022, resulting in two single-chord events, four double-chord detections, and three events with between 3 and 61 positive chords. We derived the occultation light curves using differential aperture photometry, from which the star ingress and egress instants were calculated. Using 13 selected chords from the 8 August 2020 event, we determined the global elliptical limb of 2002 MS4. The best-fit ellipse, combined with the object's rotational information from the literature, sets constraints on the object's size, shape, and albedo. Additionally, we developed a new method to characterize the topography features on the object's limb. Results: The global limb has a semi-major axis of a′ = 412 ± 10 km, a semi-minor axis of b′= 385 ± 17 km, and the position angle of the minor axis is 121° ± 16°. From this instantaneous limb, we obtained 2002 MS4's geometric albedo of pV = 0.1 ± 0.025, using HV = 3.63 ± 0.05 mag and a projected area-equivalent diameter of 796 ± 24 km. Significant deviations from the fitted ellipse in the northernmost limb were detected from multiple sites, highlighting three distinct topographic features: one 11 km depth depression, followed by a 25−5+4 km height elevation next to a crater-like depression, with an extension of 322 ± 39 km and 45.1 ± 1.5 km deep. Conclusions: Our results indicate the presence of an object that is ≈138 km smaller in diameter than that derived from thermal data, possibly indicating the presence of a thus-far unknown satellite. However, within the error bars, the geometric albedo in the V-band is in agreement with the results published in the literature, even with the radiometric-derived albedo. This stellar occultation has allowed for the first multichord measurement of a large topography in a TNO. Tables B.1-B.5 are available at the CDS via anonymous ftp to cdsarc.cds.unistra.fr (ftp://130.79.128.5) or via https://cdsarc.cds.unistra.fr/viz-bin/cat/J/A+A/678/A167
Context.
The physical characterization of trans-Neptunian objects is essential for improving our understanding of the formation and evolution of our Solar System. Stellar occultation is a ...ground-based technique that can be successfully used to determine some of the TNOs’ fundamental physical properties with high precision, such as size and shape.
Aims.
This work is aimed at constraining the size, shape, and geometric albedo of the dwarf planet candidate (307261) 2002 MS
4
through the analysis of nine stellar occultation events. Using multichord detection, we also study the object’s topography by analyzing the obtained limb and residuals between the observed chords and the best-fit ellipse.
Metods.
We predicted and organized the observational campaigns of nine stellar occultations by 2002 MS
4
between 2019 and 2022, resulting in two single-chord events, four double-chord detections, and three events with between 3 and 61 positive chords. We derived the occultation light curves using differential aperture photometry, from which the star ingress and egress instants were calculated. Using 13 selected chords from the 8 August 2020 event, we determined the global elliptical limb of 2002 MS
4
. The best-fit ellipse, combined with the object’s rotational information from the literature, sets constraints on the object’s size, shape, and albedo. Additionally, we developed a new method to characterize the topography features on the object’s limb.
Results.
The global limb has a semi-major axis of
a
′ = 412 ± 10 km, a semi-minor axis of
b
′= 385 ± 17 km, and the position angle of the minor axis is 121° ± 16°. From this instantaneous limb, we obtained 2002 MS
4
’s geometric albedo of
p
V
= 0.1 ± 0.025, using
H
V
= 3.63 ± 0.05 mag and a projected area-equivalent diameter of 796 ± 24 km. Significant deviations from the fitted ellipse in the northernmost limb were detected from multiple sites, highlighting three distinct topographic features: one 11 km depth depression, followed by a 25
−5
+4
km height elevation next to a crater-like depression, with an extension of 322 ± 39 km and 45.1 ± 1.5 km deep.
Conclusions.
Our results indicate the presence of an object that is ≈138 km smaller in diameter than that derived from thermal data, possibly indicating the presence of a thus-far unknown satellite. However, within the error bars, the geometric albedo in the V-band is in agreement with the results published in the literature, even with the radiometric-derived albedo. This stellar occultation has allowed for the first multichord measurement of a large topography in a TNO.
Summary Background Metachromatic leukodystrophy (a deficiency of arylsulfatase A ARSA) is a fatal demyelinating lysosomal disease with no approved treatment. We aimed to assess the long-term outcomes ...in a cohort of patients with early-onset metachromatic leukodystrophy who underwent haemopoietic stem-cell gene therapy (HSC-GT). Methods This is an ad-hoc analysis of data from an ongoing, non-randomised, open-label, single-arm phase 1/2 trial, in which we enrolled patients with a molecular and biochemical diagnosis of metachromatic leukodystrophy (presymptomatic late-infantile or early-juvenile disease or early-symptomatic early-juvenile disease) at the Paediatric Clinical Research Unit, Ospedale San Raffaele, in Milan. Trial participants received HSC-GT, which consisted of the infusion of autologous HSCs transduced with a lentiviral vector encoding ARSA cDNA, after exposure-targeted busulfan conditioning. The primary endpoints of the trial are safety (toxicity, absence of engraftment failure or delayed haematological reconstitution, and safety of lentiviral vector-tranduced cell infusion) and efficacy (improvement in Gross Motor Function Measure GMFM score relative to untreated historical controls, and ARSA activity, 24 months post-treatment) of HSC-GT. For this ad-hoc analysis, we assessed safety and efficacy outcomes in all patients who had received treatment and been followed up for at least 18 months post-treatment on June 1, 2015. This trial is registered with ClinicalTrials.gov , number NCT01560182. Findings Between April, 2010, and February, 2013, we had enrolled nine children with a diagnosis of early-onset disease (six had late-infantile disease, two had early-juvenile disease, and one had early-onset disease that could not be definitively classified). At the time of analysis all children had survived, with a median follow-up of 36 months (range 18–54). The most commonly reported adverse events were cytopenia (reported in all patients) and mucositis of different grades of severity (in five of nine patients grade 3 in four of five patients). No serious adverse events related to the medicinal product were reported. Stable, sustained engraftment of gene-corrected HSCs was observed (a median of 60·4% range 14·0–95·6 lentiviral vector-positive colony-forming cells across follow-up) and the engraftment level was stable during follow-up; engraftment determinants included the duration of absolute neutropenia and the vector copy number of the medicinal product. A progressive reconstitution of ARSA activity in circulating haemopoietic cells and in the cerebrospinal fluid was documented in all patients in association with a reduction of the storage material in peripheral nerve samples in six of seven patients. Eight patients, seven of whom received treatment when presymptomatic, had prevention of disease onset or halted disease progression as per clinical and instrumental assessment, compared with historical untreated control patients with early-onset disease. GMFM scores for six patients up to the last follow-up showed that gross motor performance was similar to that of normally developing children. The extent of benefit appeared to be influenced by the interval between HSC-GT and the expected time of disease onset. Treatment resulted in protection from CNS demyelination in eight patients and, in at least three patients, amelioration of peripheral nervous system abnormalities, with signs of remyelination at both sites. Interpretation Our ad-hoc findings provide preliminary evidence of safety and therapeutic benefit of HSC-GT in patients with early-onset metachromatic leukodystrophy who received treatment in the presymptomatic or very early-symptomatic stage. The results of this trial will be reported when all 20 patients have achieved 3 years of follow-up. Funding Italian Telethon Foundation and GlaxoSmithKline.
The main scientific goal of TESS is to find planets smaller than Neptune around stars that are bright enough to allow for further characterization studies. Given our current instrumentation and ...detection biases, M dwarfs are prime targets in the search for small planets that are in (or near) the habitable zone of their host star. In this work, we use photometric observations and CARMENES radial velocity measurements to validate a pair of transiting planet candidates found by TESS. The data were fitted simultaneously, using a Bayesian Markov chain Monte Carlo (MCMC) procedure and taking into account the stellar variability present in the photometric and spectroscopic time series. We confirm the planetary origin of the two transiting candidates orbiting around TOI-2095 (LSPM J1902+7525). The star is a nearby M dwarf (\(d = 41.90 \pm 0.03\) pc, \(T_{\rm eff} = 3759 \pm 87\) K, \(V = 12.6\) mag), with a stellar mass and radius of \(M_\star = 0.44 \pm 0.02 \; M_\odot\) and \(R_\star = 0.44 \pm 0.02 \; R_\odot\), respectively. The planetary system is composed of two transiting planets: TOI-2095b, with an orbital period of \(P_b = 17.66484 \pm (7\times 10^{-5})\) days, and TOI-2095c, with \(P_c = 28.17232 \pm (14\times 10^{-5})\) days. Both planets have similar sizes with \(R_b = 1.25 \pm 0.07 \; R_\oplus\) and \(R_c = 1.33 \pm 0.08 \; R_\oplus\) for planet b and planet c, respectively. Although we did not detect the induced RV variations of any planet with significance, our CARMENES data allow us to set stringent upper limits on the masses of these objects. We find \(M_b < 4.1 \; M_\oplus\) for the inner and \(M_c < 7.4 \; M_\oplus\) for the outer planet (95% confidence level). These two planets present equilibrium temperatures in the range of 300-350 K and are close to the inner edge of the habitable zone of their star.
In this study, we characterize the natural course of metachromatic leukodystrophy (MLD), explore intra/inter group differences, and identify biomarkers to monitor disease progression. This is a ...longitudinal observational study. Genotype and characteristics at disease onset were recorded. Time‐to‐event analyses were performed to assess time to major disease‐related milestones in different subgroups. Longitudinal trajectories of nerve conduction velocities (NCV), brain MRI score, and brainstem auditory evoked responses (BAERs) were described. We recruited 22 late‐infantile, 14 early‐juvenile, 5 late‐juvenile, and 4 adult MLD patients. Thirty‐four were prospectively evaluated (median FU time 43 months). In late‐infantile patients, the attainment of independent walking was associated with a later age at dysphagia. In early‐juvenile, the presence of isolated cognitive impairment at onset was not a favorable prognostic factor. Late‐infantile and early‐juvenile subjects showed similar rapid loss of ambulation and onset of seizures, but late‐infantile displayed earlier loss of trunk control, dysphagia, and death. We found significant differences in all major disease‐related milestones (except death) between early‐juvenile and late‐juvenile patients. Late‐juvenile and adult patients both presented with a predominant cognitive impairment, mild/no peripheral neuropathy, lower brain MRI score at plateau compared to LI/EJ, and later cerebellar involvement. NCV and BAER were consistently severely abnormal in late‐infantile but not in older subjects, in whom both NCV and BAER were variably affected, with no deterioration over time in some cases. This study clarifies intra/inter group differences between MLD subtypes and provides additional indications regarding reliable clinical and instrumental tools to monitor disease progression and to serve as areference to evaluate the efficacy of future therapeutic interventions inthe different MLD variants.
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