Abstract
OrthoDB (https://www.orthodb.org) provides evolutionary and functional annotations of orthologs. This update features a major scaling up of the resource coverage, sampling the genomic ...diversity of 1271 eukaryotes, 6013 prokaryotes and 6488 viruses. These include putative orthologs among 448 metazoan, 117 plant, 549 fungal, 148 protist, 5609 bacterial, and 404 archaeal genomes, picking up the best sequenced and annotated representatives for each species or operational taxonomic unit. OrthoDB relies on a concept of hierarchy of levels-of-orthology to enable more finely resolved gene orthologies for more closely related species. Since orthologs are the most likely candidates to retain functions of their ancestor gene, OrthoDB is aimed at narrowing down hypotheses about gene functions and enabling comparative evolutionary studies. Optional registered-user sessions allow on-line BUSCO assessments of gene set completeness and mapping of the uploaded data to OrthoDB to enable further interactive exploration of related annotations and generation of comparative charts. The accelerating expansion of genomics data continues to add valuable information, and OrthoDB strives to provide orthologs from the broadest coverage of species, as well as to extensively collate available functional annotations and to compute evolutionary annotations. The data can be browsed online, downloaded or assessed via REST API or SPARQL RDF compatible with both UniProt and Ensembl.
In this paper, we present evidence from the Current Population Survey examining the effects of the COVID-19 crisis on parental status and gender inequalities in employment in the United States. We ...show that the drop in the employment rate in post-outbreak months was largely driven by mass layoffs and not by workers quitting their jobs. Results from fixed-effects regression models show a strong fatherhood premium in the likelihood of being laid off for post-outbreak months compared to mothers, men without children, and women without children. We also found that the “fatherhood premium” was higher among lower-educated and mid-educated workers. These findings show that gaps in layoff rates exacerbated pre-existing forms of parental status and gender inequality in employment. Possible mechanisms are discussed, but more work is needed to explain why employers were less likely to lay off fathers following the outbreak, and the short- and long-term consequences of the COVID-19 pandemic in reinforcing parental status and gender inequality in employment in the United States.
A large body of sociological research has shown that racial minorities and women experience significant disadvantages in the labor market. In this visualization, the author presents evidence from the ...Current Population Survey examining the effects of the coronavirus disease 2019 crisis on racial and gender inequalities in employment in the United States among prime-age workers. The author shows that the white-nonwhite gap in employment increased significantly during the post-outbreak period. Results from individual fixed-effects regression models show a strong white male advantage in the likelihood of being laid off for post-outbreak months compared with women, black men, Hispanic men, and Asian men.
Does racial wage discrimination increase during economic downturns? In this article, the author tests empirically the association between economic conditions and racial wage discrimination for black, ...Hispanic, and Asian workers. Using data from the Current Population Survey, the author finds that the wage gap between Hispanics and whites, and between Asians and whites, increases with the job-seeker rate and unemployment rate. However, the wage gap between black and white workers increases slightly with the unemployment rate and does not change at all with the job-seeker rate. The author advances the concept of “wage discrimination flexibility” to argue that racial wage discrimination against black workers is more rigid and resistant to changes in economic environments, whereas wage discrimination against Hispanics and Asians is more flexible and responsive to economic conditions. The author discusses the implications of these findings for theories of discrimination and for policies aiming to foster equal opportunities in the labor market.
Blood-feeding arthropods are vectors of infectious diseases such as dengue, Zika, Chagas disease, and malaria 1, and vector control is essential to limiting disease spread. Because these arthropods ...ingest very large amounts of blood, a protein-rich meal, huge amounts of amino acids are produced during digestion. Previous work on Rhodnius prolixus, a vector of Chagas disease, showed that, among all amino acids, only tyrosine degradation enzymes were overexpressed in the midgut compared to other tissues 2. Here we demonstrate that tyrosine detoxification is an essential trait in the life history of blood-sucking arthropods. We found that silencing Rhodnius tyrosine aminotransferase (TAT) and 4-hydroxyphenylpyruvate dioxygenase (HPPD), the first two enzymes of the phenylalanine/tyrosine degradation pathway, caused the death of insects after a blood meal. This was confirmed by using the HPPD inhibitor mesotrione, which selectively killed hematophagous arthropods but did not affect non-hematophagous insects. In addition, mosquitoes and kissing bugs died after feeding on mice that had previously received a therapeutic effective oral dose (1 mg/kg) of nitisinone, another HPPD inhibitor used in humans for the treatment of tyrosinemia type I 3. These findings indicate that HPPD (and TAT) can be a target for the selective control of blood-sucking disease vector populations. Because HPPD inhibitors are extensively used as herbicides and in medicine, these compounds may provide an alternative less toxic to humans and more environmentally friendly than the conventional neurotoxic insecticides that are currently used, with the ability to affect only hematophagous arthropods.
•Blood-sucking insects ingest blood meals several-fold their weight before feeding•Toxic concentrations of tyrosine are produced by blood digestion•Capacity to detoxify excess tyrosine is an essential life trait for these animals•Inhibition of tyrosine degradation selectively kills blood-sucking arthropods
Sterkel et al. show that blood digestion by blood-sucking insects produces toxic amounts of tyrosine, and that detoxification of an excess of this amino acid is an adaptation to a blood-feeding way of life. Inhibition of tyrosine degradation selectively kills these insects, allowing the design of compounds that target only disease-transmitting insects.
The use of large-scale antenna arrays grants considerable benefits in energy and spectral efficiency to wireless systems due to spatial resolution and array gain techniques. By assuming a dominant ...line-of-sight environment in a massive multiple-input multiple-output scenario, we derive analytical expressions for the sum-capacity. Then, we show that convenient simplifications on the sum-capacity expressions are possible when working at low and high signal-to-noise ratio regimes. Furthermore, in the case of low and high signal-to-noise ratio regimes, it is demonstrated that the Gamma probability density function can approximate the probability density function of the instantaneous channel sum-capacity as the number of served devices and base station antennas grows, respectively. A second important demonstration presented in this work is that a Gamma probability density function can also be used to approximate the probability density function of the summation of the channel's singular values as the number of devices increases. Finally, it is important to highlight that the presented framework is useful for a massive number of Internet of Things devices as we show that the transmit power of each device can be made inversely proportional to the number of base station antennas.
One of the primary challenges in biodiversity conservation is the limited knowledge of the taxonomy and geographical distribution of species, particularly in megadiverse regions such as the Amazon
In ...this context, the present study aimed to mitigate the Linnean and Wallacean shortfalls within the Family Leptophlebiidae (Ephemeroptera: Insecta) in the Brazilian Amazon, identify priority areas for conducting faunal inventories and enhance taxonomic knowledge of this group.
Utilizing both primary Leptophlebiidae data collected in the state of Pará, and secondary data compiled from the literature on species occurring in the Amazon, we employed Species Distribution Modelling to identify areas with higher potential species richness and priority areas for faunal inventories in the Brazilian Amazon of the family.
For the primary data, 2,120 specimens were identified, representing 16 genera and 28 species. Among the 28 species analysed, 7 constitute new records for the state of Pará. Additionally, two new genera, five new species and six morphospecies were identified, significantly expanding the taxonomic landscape of the family in the state. Maps depicting potential species richness and priority areas for faunal inventories in the Brazilian Amazon generated from the Modelling indicated that the states of Pará and the western region of Maranhão exhibit high potential species richness, with overlapping areas identified as priorities for Leptophlebiidae faunal inventories.
In this context, this study represents a substantial advancement in the taxonomic knowledge of Leptophlebiidae in the state of Pará and the Amazon, underscoring the urgency of research and biodiversity conservation efforts for the group in the region. Furthermore, the identification of new taxa emphasizes the need for ongoing collaborative efforts to mitigate Linnean and Wallacean shortfalls.
Application of machine learning models to study land-cover change is typically restricted to the change detection of categorical, i.e. classified, land-cover data. In this study, our aim is to extend ...the utility of such models to predict the spectral band information of satellite images. A Random Forests (RF)-based machine learning model is trained using topographic and historical climatic variables as inputs to predict the spectral band values of high-resolution satellite imagery across two large sites in the western United States, New Mexico (10,570 km
2
), and Washington (9400 km
2
). The model output is used to obtain a true colour photorealistic image and an image showing the normalized difference vegetation index values. We then use the trained model to explore what the land cover might look like for a climate change scenario during the 2061-2080 period. The RF model achieves high validation accuracy for both sites during the training phase, with the coefficient of determination (R
2
) = 0.79 for New Mexico site and R
2
= 0.73 for Washington site. For the climate change scenario, prominent land-cover changes are characterized by an increase in the vegetation cover at the New Mexico site and a decrease in the perennial snow cover at the Washington site. Our results suggest that direct prediction of spectral band information is highly beneficial due to the ability it provides for deriving ecologically relevant products, which can be used to analyse land-cover change scenarios from multiple perspectives.
Blood-sucking insects are responsible for the transmission of several important disease-causing organisms such as viruses, bacteria, and protozoans. The hematophagous hemipteran Rhodnius prolixus is ...one of the most important vectors of Trypanosoma cruzi, the etiological agent of Chagas disease. Due to the medical importance of this insect, it has been used as a study model in physiology and biochemistry since the 1930s. Artificial feeding has been recognized as a feasible and a more ethical alternative method of feeding these hematophagous insects. To prevent clotting after blood collection defibrination or treatment with anticoagulants are necessary. Although anticoagulants have been routinely used for stabilizing the collected blood, there is a gap in demonstration of the effects of using anticoagulants on the feeding and development of the hematophagous insect Rhodnius prolixus. In this study, we compared the survival rate, molting efficiency, fertility, and infection development between insects that were fed on blood containing three different anticoagulants (citrate, EDTA, and heparin). We observed that fifth instar nymphs that were fed on blood containing EDTA and citrate could not perform digestion properly, which resulted in molting inefficiency. Adult insects that were fed on EDTA-containing blood laid lower number of eggs, and also had a diminished egg hatch percentage. When we delivered T. cruzi parasites in blood containing citrate or EDTA to the insects, a lower number of parasites and metacyclic trypomastigotes was observed in the intestine compared to the group fed on heparin-containing blood. Since heparin could potentially inhibit DNA polymerase activity in DNA samples extracted from the intestine, we analyzed different heparin concentrations to determine which one is the best for use as an anticoagulant. Concentrations ranging between 2.5 and 5 U/mL were able to inhibit coagulation without severely impairing DNA polymerase activity, thus indicating that this should be considered as the range of use for feeding experiments. Our results suggest that among the three anticoagulants tested, heparin can be recommended as the anticoagulant of choice for R. prolixus feeding experiments.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Guatteria olivacea R. E. Fries (synonym Guatteria punctata (Aubl.) R.A. Howard) is a tree of 10–27 m tall popularly known as “envira-bobó”, “envira-fofa”, “envireira”, “embira”, “embira-branca”, ...“embira-preta”, envira-branca”, and “envira-preta”, which can be found in the Brazilian Amazon biome. In this study, we evaluated the cytotoxic and antitumor effects of the essential oil (EO) obtained from the leaves of G. olivacea against liver cancer using HepG2 cells as a model. EO was obtained using a hydrodistillation Clevenger-type apparatus and was qualitatively and quantitatively characterized using GC–MS and GC–FID, respectively. The alamar blue assay was used to assess the cytotoxic potential of EO in a panel of human cancer cell lines and human non-cancerous cells. In HepG2 cells treated with EO, YO-PRO-1/propidium iodide staining, cell cycle distribution, and reactive oxygen species (ROS) were examined. In C.B-17 SCID mice with HepG2 cell xenografts, the efficacy of the EO (20 and 40 mg/kg) was tested in vivo. GC–MS and GC–FID analyses showed germacrene D (17.65%), 1-epi-cubenol (13.21%), caryophyllene oxide (12.03%), spathulenol (11.26%), (E)-caryophyllene (7.26%), bicyclogermacrene (5.87%), and δ-elemene (4.95%) as the major constituents of G. olivacea leaf EO. In vitro cytotoxicity of EO was observed, including anti-liver cancer action with an IC50 value of 30.82 μg/mL for HepG2 cells. In HepG2 cells, EO treatment increased apoptotic cells and DNA fragmentation, without changes in ROS levels. Furthermore, the EO inhibited tumor mass in vivo by 32.8–57.9%. These findings suggest that G. olivacea leaf EO has anti-liver cancer potential.