Computational integrative analysis has become a significant approach in the data-driven exploration of biological problems. Many integration methods for cancer subtyping have been proposed, but ...evaluating these methods has become a complicated problem due to the lack of gold standards. Moreover, questions of practical importance remain to be addressed regarding the impact of selecting appropriate data types and combinations on the performance of integrative studies. Here, we constructed three classes of benchmarking datasets of nine cancers in TCGA by considering all the eleven combinations of four multi-omics data types. Using these datasets, we conducted a comprehensive evaluation of ten representative integration methods for cancer subtyping in terms of accuracy measured by combining both clustering accuracy and clinical significance, robustness, and computational efficiency. We subsequently investigated the influence of different omics data on cancer subtyping and the effectiveness of their combinations. Refuting the widely held intuition that incorporating more types of omics data always produces better results, our analyses showed that there are situations where integrating more omics data negatively impacts the performance of integration methods. Our analyses also suggested several effective combinations for most cancers under our studies, which may be of particular interest to researchers in omics data analysis.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Herein, we reported a label-free and reliable photoelectrochemical (PEC) platform for highly selective monitoring of norepinephrine (NE) based on metallic Bi nanoparticles anchored on hollow porous ...ZnSnO3 microspheres (3D Bi/ZnSnO3) via a simple solvothermal strategy. The designed 3D Bi/ZnSnO3 Schottky junction exhibited a unique photoanodic response toward NE among other catechol derivatives, such as epinephrine (EP) and dopamine (DA), and effectively shielded the interference from thirteen coexisting biomolecules like uric acid (UA) and ascorbic acid (AA). High selectivity and excellent sensitivity could be correlated to the unique chelating coordination interaction between NE and Zn2+ at surface sites as well as the efficient carrier separation of Bi/ZnSnO3, thereby developing a novel "signal-on" label-free and selective strategy for NE detection. The proposed Bi/ZnSnO3-based PEC sensor achieved remarkable NE biosensing with a low detection limit of 0.68 nmol L-1 and a wide response ranging from 0.002 to 350.0 μmol L-1. The applicability of this biosensor was realized for the selective analysis of NE in human serum, human urine and injection samples, laying the foundation for the label-free PEC monitoring of NE in biological fluids.
Herein, we reported a label-free and reliable photoelectrochemical (PEC) platform for highly selective monitoring of norepinephrine (NE) based on metallic Bi nanoparticles anchored on hollow porous ...ZnSnO
3
microspheres (3D Bi/ZnSnO
3
)
via
a simple solvothermal strategy. The designed 3D Bi/ZnSnO
3
Schottky junction exhibited a unique photoanodic response toward NE among other catechol derivatives, such as epinephrine (EP) and dopamine (DA), and effectively shielded the interference from thirteen coexisting biomolecules like uric acid (UA) and ascorbic acid (AA). High selectivity and excellent sensitivity could be correlated to the unique chelating coordination interaction between NE and Zn
2+
at surface sites as well as the efficient carrier separation of Bi/ZnSnO
3
, thereby developing a novel "signal-on" label-free and selective strategy for NE detection. The proposed Bi/ZnSnO
3
-based PEC sensor achieved remarkable NE biosensing with a low detection limit of 0.68 nmol L
−1
and a wide response ranging from 0.002 to 350.0 μmol L
−1
. The applicability of this biosensor was realized for the selective analysis of NE in human serum, human urine and injection samples, laying the foundation for the label-free PEC monitoring of NE in biological fluids.
Proposed mechanism of the label-free PEC biosensing platform for selective detection of norepinephrine.
Concentrating and spectral beam splitting technology towards the efficient utilization of full-spectrum solar energy has been widely concerned. This paper proposes a novel non-confocal two-stage ...parabolic dish concentrating photovoltaic/thermal (NTPD-CPVT) hybrid system which offers access to the alleviation of photovoltaic heat load and the two-stage heating of heat transfer fluid through a specially developed spectral beam splitter. The thermodynamic and optical properties of the system are analyzed via the comprehensive model including ray tracing, energy flux evaluation and photovoltaic/thermal hybrid energy evaluation. Results indicate that the satisfied spot uniformity and optical efficiency of 80.7% could be obtained by non-confocal optimization, and the NTPD-CPVT hybrid system shows stable overall energy efficiency over 20.9% under different irradiance conditions. Based on the comprehensive comparison, the introduction of a spectral beam splitter promotes the photovoltaic/thermal module efficiency and the system exergy efficiency by 17.6% and 10.2%, and effectively shortens the payback period. Furthermore, the reference values of implemental parameters such as error tolerances and heat transfer coefficients are provided to guarantee the optical and economic performance of the system. The research results support the potential of the NTPD-CPVT hybrid system in improving the cascade use of solar spectrum and guide the construction of future prototypes.
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Gouty arthritis evokes joint pain and inflammation. Mechanisms driving gout pain and inflammation remain incompletely understood. Here we show that CXCL5 activates CXCR2 expressed on nociceptive ...sensory neurons to drive gout pain and inflammation. CXCL5 expression was increased in ankle joints of gout arthritis model mice, whereas CXCR2 showed expression in joint-innervating sensory neurons. CXCL5 activates CXCR2 expressed on nociceptive sensory neurons to trigger TRPA1 activation, resulting in hyperexcitability and pain. Neuronal CXCR2 coordinates with neutrophilic CXCR2 to contribute to CXCL5-induced neutrophil chemotaxis via triggering CGRP- and substance P-mediated vasodilation and plasma extravasation. Neuronal Cxcr2 deletion ameliorates joint pain, neutrophil infiltration and gait impairment in model mice. We confirmed CXCR2 expression in human dorsal root ganglion neurons and CXCL5 level upregulation in serum from male patients with gouty arthritis. Our study demonstrates CXCL5-neuronal CXCR2-TRPA1 axis contributes to gouty arthritis pain, neutrophil influx and inflammation that expands our knowledge of immunomodulation capability of nociceptive sensory neurons.
•Preparation of biochar carriers is the effective utilization of waste biomass.•ZrO2-MoO3/biochar catalysts can upgrade heavy oil at lower temperatures.•The viscosity reduction rate of heavy oil is ...86.1% at 200 ℃.•The temperature of heavy oil catalytic aquathermolysis is reduced by 20 ℃.
Aquathermolysis is one of the thermochemical reactions in the heavy oil thermal recovery process. The key issue, i.e., the high temperature required by aquathermolysis, can be reduced by the involvement of catalysts. However, the effective working temperature of catalysts is still above 240 ℃, which needs to be further reduced to enhance energy efficiency. Considering that biochar has a high specific surface area, abundant functional groups, a wide temperature window, and can be easily modified, a series of ZrO2-MoO3-based catalysts loaded on raw or three modified lotus stem biochar were studied to investigate heavy oil catalytic viscosity reduction at low temperatures. Physicochemical properties of catalysts were investigated by a variety of characterization methods. The performance of four kinds of catalysts and the influence of reaction conditions (temperature and time) on aquathermolysis were evaluated. The results showed that silane coupling agent KH570 modified biochar (KH570-MLSB) has a higher specific surface area and more functional groups, which help the loading of active components and the dispersion in heavy oil. ZrO2 can form coordination compounds with S atoms in C-S, which greatly weakens the bond energy of C-S and reduces the activation energy of the aquathermolysis. The coordination bonding energy with S of Mo is weaker than that of Zr. The main role of MoO3 is to inhibit the formation of coke. Therefore, catalyst Ⅳ, which consists of KH570-MLSB, ZrO2, and MoO3, has the best catalytic effect at 200 ℃. In addition, the optimum reaction time of the catalyst is 24 h.
Objective: Respiratory infections or colonization of Acinetobacter baumannii (Ab) are common in clinical practice but are treated differently. Early identification of Ab infection and colonization ...reduces the risk of antibiotic mismatch but objective laboratory indicators to distinguish between bacterial infections and colonization are lacking. To distinguish infection and colonization of Ab, we tested the role of two biomarkers, triggering receptor expressed on myeloid cells-1 (TREM-1) and hemolysin coregulated protein. Methods: A total of 96 inpatients with Ab were divided into infection and colonization groups. Blood samples were collected on days 1, 2, 3, 5, 8, and 10 and daily maximum body temperature was recorded. Polymerase Chain Reaction and Reverse Transcription Polymerase Chain Reaction were used to detect the presence and expression levels of the hcp gene in Ab clinical isolates. Results : sTREM-1 and procalcitonin (PCT) levels on days 1 to 10 and neutrophil classification (N%) on days 1 to 3 were different ( P < 0.05) in the infection group and colonization group. Receiver operating characteristic (ROC) curves showed significant differences in N% and sTREM-1 on days 2 and 3 ( P < 0.01). sTREM-1 had the highest AUC ROC on days 1, 2, and 3 of all the markers. On day 1, the ROC curve of "WBC&N%&PCT&sTREM-1" was statistically different from individual indices (white blood cell count, N%, and PCT; P < 0.05) and was equal to the ROC curve of sTREM-1 ( P > 0.05). Thirty five of 96 patients were classified as infection group and 61 as colonization group with hcp gene detection rates of 71.43% (25/35) and 31.15% (19/61), respectively. No differences in hcp gene presence and transcript levels were found between two groups ( P > 0.05). Conclusions: Dynamic monitoring of sTREM-1 and PCT is valuable in identifying Ab infection and colonization. sTREM-1 can be improved by combination with multiple biomarkers in the early stage for identification of infection and colonization. The hcp gene was more likely to be present in the infection cohort.
In this article, we propose a facile method for synthesis of K2SiF6:Mn4+ phosphor and discuss its promising application in warm‐white light emitting diodes (LED). The K2SiF6:Mn4+ was synthesized from ...SiO2 powders through redox reaction in HF/KMnO4 solution. The optical properties of LEDs containing different ratios of K2SiF6:Mn4+ phosphor and commercial Ce3+‐doped garnets (YAG‐40) yellow–green phosphor were studied. A warm‐white LED, with color temperature of 3510 K and color rendering index of 90.9 and efficacy of 81.56 lm/W was demonstrated.
Carbon, as a promising commercial material, has captured a lot of attention for use in sodium-ion batteries (SIBs). But, their energy-storage mechanism is still confusing, especially the effect of an ...ester/ether-based electrolyte. Attractive because of its rich resources and low cost, natural coal has been regarded as an important candidate. Through optimization of pyrolysis temperature, a series of significant traits towards improvements in energy-storage ability were tailored, including graphitization degree, interlayer distance and hetero-atoms (oxygen, sulfur). As an SIB anode, the optimized sample delivers a capacity of 252 mA h g
−1
in an ester-based electrolyte and 325 mA h g
−1
in ether-based systems. Through detailed analysis, it could be confirmed that the relatively small energy of dissolution would induce an enhancement in adsorption behavior. The flexible Na-solvation shells weaken the combining energy in a graphitized interlayer and compact ring-structure, resulting in improved capacity. More significantly, with the assistance of an ether-based solvation shell, the strong force between quasi-metallic Na and carbon atoms is reduced, facilitating the stabilization of the low-voltage capacity contribution even at high current density. Given this, the results are anticipated to provide an in-depth understanding of Na-ion diffusion with a solvation shell, and an effective strategy for natural coal-based carbon materials.
Coal-based materials were produced by pyrolyzing at 1000 °C. The use of ether-based electrolytes offered advantages, including low solvation enthalpy and viscosity, which resulting material demonstrated a high capacity of up to 325 mA h g−
1
.
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