Lactic acid bacteria is an important dominant bacterial community in the intestinal tract, which can regulate the intestinal environment and maintain the balance of intestinal flora. However, there ...are few studies on Tibetan pig lactic acid bacteria. The purpose of this study was to isolate and identify lactic acid bacteria from Tibetan pig feces, and to provide experimental materials for the development and utilization of Tibetan pig probiotics. Seventeen strains of Lactobacillus isolated from Tibetan pig manure were identified by physiology, biochemistry, 16S rRNA molecular biology and acid and bile salt tolerance tests. The results showed that, screened strain is Lactobacillus Roy's F1. Tibetan pig source Roy's Lactobacillus F1 showed the ability to grow maximum when the inoculation was 1%, the temperature 37 °C and pH = 7. This strain depicted a strong tolerance for artificial gastric and intestinal juice. Roy's Lactobacillus F1 can inhibit the growth of Staphylococcus aureus and Salmonella. Lactobacillus reuteri F1 also showed a strong hydrophobicity and self-agglutination ability. It is sensitive to butamicane, chloramphenicol, erythromycin, cefazolin, norfloxacin, cotrimoxazole and other antibiotics, and has resistance for ampicillin, gentamicin, ciprofloxacin, penicillin and so on. In this study, one strain of Tibetan Porcine Lactobacillus was screened, obtained, and its biological characteristics were found good, which could provide reference and support for the development and utilization of Tibetan porcine probiotics.
Muscle growth stands as a pivotal economic trait within pig production, governed by a complex interplay of multiple genes, each playing a role in its quantitative manifestation. Understanding the ...intricate regulatory mechanisms of porcine muscle development is crucial for enhancing both pork yield and quality. This study used the GSE99749 dataset downloaded from the GEO database, conducting a detailed analysis of the RNA-seq results from the longissimus dorsi muscle (LD) of Tibetan pigs (TP), Wujin pigs (WJ) and large white pigs (LW) at 60 days of gestation, representing diverse body sizes and growth rates. Comparative analyses between TPvsWJ and TPvsLW, along with differential gene expression (DEG) analysis, functional enrichment analysis, and protein-protein interaction (PPI) network analysis, revealed 1048 and 1157 significantly differentially expressed genes (
< 0.001) in TPvsWJ and TPvsLW, respectively. With stricter screening criteria, 37 DEGs were found to overlap between the 2 groups. PPI analysis identified
,
, and
as the three core genes. This article focuses on exploring the
gene. Molecular-level experimental validation, through overexpression and interference of the
gene combined with EDU staining experiments, demonstrated that overexpression of
significantly promoted the proliferation of porcine skeletal muscle satellite cells (PSMSC), while interference with
inhibited their proliferation. Furthermore, by examining the effects of overexpressing and interfering with the
gene on the muscle hypertrophy marker
gene and the muscle degradation marker
gene, the pivotal role of the
gene in promoting muscle growth and preventing muscle degradation was further confirmed. These findings offer a new perspective on the molecular mechanisms behind porcine muscle growth and development, furnishing valuable data and insights for muscle biology research.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
The
gene, which is an actin-binding protein, is prevalent in eukaryotic cells and is abundantly present in various pathways associated with plateau hypoxia adaptation. Tibetan pigs, which have ...inhabited high altitudes for extended periods, provide an excellent research population for investigating plateau hypoxia adaptation.
This study focused on Tibetan pigs and Yorkshire pigs residing in Nyingchi, Tibet. The blood physiological data of Tibetan pigs were found to be significantly higher than those of Yorkshire pigs, including RBC, HGB, HCT, MCH, and MCHC. The SNP analysis of the
gene identified six sites with mutations only present in Tibetan pigs. Notably, the transcription factors at sites C-489T, C-274T, and A-212G were found to be altered, and these sites are known to be associated with hypoxia adaptation and blood oxygen transportation. The mRNA expression of the
gene exhibited highly significant differences in several tissues, with the target proteins predominantly higher in the Yorkshire pig compared to the Tibetan pig. Specifically, a notable difference was observed in the lung tissues. Immunohistochemistry analysis revealed high expression levels of
proteins in the lung tissues of both Tibetan and Yorkshire pigs, primarily localized in the cytoplasm and cell membrane.
The
gene plays a significant role in regulating hypoxia adaptation in Tibetan pigs. This study provides a theoretical basis for the conservation and utilization of Tibetan pig resources, the breeding of highland breeds, epidemic prevention and control, and holds great importance for the development of the highland livestock economy.
Muscle growth is essential for the economic sustainability of the swine industry. Gaining a deeper understanding of pig muscle development is crucial for improving both the quantity and quality of ...pork production. This study employed RNA-seq technology to conduct a transcriptomic analysis of the longissimus dorsi muscle (LD) in three different pig breeds with varying body sizes and growth rates: Tibetan pigs (TP), Wujin pigs (WJ), and large white pigs (LW). Significant differences in gene expression were observed among the different breeds. We identified that myosin light chain 4 (MYL4) plays a role in influencing muscle growth. Experimental validation confirmed that MYL4 promotes skeletal muscle cell proliferation and inhibits degradation. These findings reveal the molecular mechanisms that control muscle growth and provide valuable insights for improving pig breeding practices, ultimately enhancing the efficiency and quality of meat production. Muscle growth stands as a pivotal economic trait within pig production, governed by a complex interplay of multiple genes, each playing a role in its quantitative manifestation. Understanding the intricate regulatory mechanisms of porcine muscle development is crucial for enhancing both pork yield and quality. This study used the GSE99749 dataset downloaded from the GEO database, conducting a detailed analysis of the RNA-seq results from the longissimus dorsi muscle (LD) of Tibetan pigs (TP), Wujin pigs (WJ) and large white pigs (LW) at 60 days of gestation, representing diverse body sizes and growth rates. Comparative analyses between TPvsWJ and TPvsLW, along with differential gene expression (DEG) analysis, functional enrichment analysis, and protein–protein interaction (PPI) network analysis, revealed 1048 and 1157 significantly differentially expressed genes (p < 0.001) in TPvsWJ and TPvsLW, respectively. With stricter screening criteria, 37 DEGs were found to overlap between the 2 groups. PPI analysis identified MYL5, MYL4, and ACTC1 as the three core genes. This article focuses on exploring the MYL4 gene. Molecular-level experimental validation, through overexpression and interference of the MYL4 gene combined with EDU staining experiments, demonstrated that overexpression of MYL4 significantly promoted the proliferation of porcine skeletal muscle satellite cells (PSMSC), while interference with MYL4 inhibited their proliferation. Furthermore, by examining the effects of overexpressing and interfering with the MYL4 gene on the muscle hypertrophy marker Fst gene and the muscle degradation marker FOXO3 gene, the pivotal role of the MYL4 gene in promoting muscle growth and preventing muscle degradation was further confirmed. These findings offer a new perspective on the molecular mechanisms behind porcine muscle growth and development, furnishing valuable data and insights for muscle biology research.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Fat deposition traits are one of the key factors in pig production and breeding. The fat deposition capacity of pigs mainly affects the quality of pork and pig productivity. The aim of this study was ...to analyze the differential expression of mRNA levels in dorsal adipose tissue of Tibetan and York pigs at different growth stages using transcriptomic data to estimate key genes that regulate fat deposition in pigs. The results showed that a total of 32,747 positively expressed genes were present in the dorsal adipose tissue of the two breeds. Differentially expressed gene (DEG) screening of multiple combinations between the two breeds yielded 324 DEGS. Gene ontology (GO) biofunctional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DEGS were mainly involved in lipid metabolic pathways, steroid biosynthetic pathways and lipid biosynthetic processes, sterol biosynthetic processes, brown adipocyte differentiation, and other pathways related to lipid deposition and metabolism. The results showed that ACACA, SLC2A4 and THRSP genes positively regulated the lipid deposition ability and CHPT1 gene negatively regulated the lipid deposition ability in pigs. The results of this experiment suggest a theoretical basis for further studies on the regulatory mechanisms of fat deposition in pigs.
This study uses the method of Audio-frequency magnetotelluric sounding (EH4) to detect and analyze the deep geological structure in the Donglifang mining area. The underground spatial electricity and ...structural characteristics were effectively determined. We built an EH4-based indicator system for localizing ore bodies and summarized the relationship between the apparent resistivity anomalies and the ore bodies in the EH4 profile. The shallow veined or columnar low-resistance bodies may correspond to the low–medium temperature hydrothermal gold, lead, and zinc polymetallic ore bodies; the columnar medium-resistance or medium–low resistance bodies correspond to the silica or porphyry copper–molybdenum polymetallic ore bodies; the medium-resistance bodies correspond to the medium acidic magmatic rocks (porphyrites and porphyries). We identified eleven concealed ore bodies in the deep of the mine area, five of which are consistent with the drill holes, and the remaining six have an excellent prospect of finding
Although the delayed-type hypersensitivity (DTH) skin reaction to tuberculin is used worldwide for tuberculosis (TB) detection, it has poor diagnostic specificity due to the presence of common ...antigens in tuberculin shared by many mycobacterial species. The problem is noticed, especially in countries where the Bacillus Calmette-Gue'rin (BCG) vaccination is widely practiced. Thus, a new skin test antigen specific for the diagnosis of Mycobacterium tuberculosis (MTB) infection is urgently needed. CFP-10, a mycobacterial secretary protein that is absent in Mycobacterium bovis BCG and most other mycobacterial species including Mycobacterium avium, Mycobacterium intracellulare, has been shown to elicit cellular immune responses in MTB infected individuals and can be a good candidate for MTB specific diagnosis. We prepared recombinant MTB CFP-10, rCFP-10, and its utility as specific antigen for TB diagnosis was evaluated by skin testing in guinea pigs sensitized with M. tuberculosis, M. bovis, and M. bovis BCG. Our results show that the purified MTB rCFP-10 antigen elicits a positive skin response only in the guinea pigs sensitized with M. tuberculosis and M. bovis, and not in the animals sensitized with M. bovis BCG vaccine. The data presented in this study supports further testing of the use rCFP-10 as the specific antigen in the skin test for the diagnosis of MTB infection in humans.
A several-step tank formation method and related charging equipment have been developed for automotive lead/acid batteries. This process offers the advantages of reduced energy requirements, ...increased charging efficiency and reduced environmental problems. Results also suggest that several-step formation ameliorates the problem of premature capacity loss and extends the useful service-life of automotive batteries. This is thought to be due to the production of greater amounts of α-PbO
2 in the positive plates.
