For biomimetic applications, an artificial material is needed to be self-healing, electroactive and bio-applicable. Herein we report a strategy to build a graphene-poly(N,N-dimethyl acrylamide) ...(PDMAA) cross-linking structure based on graphene networks. The obtained hydrogel exhibits good neural compatibility, high conductivity, low impedance and efficient near-infrared-triggered photothermal self-healing behaviour owing to its unique 3-dimensional graphene-PDMAA cross-linking networks. The results indicate that the graphene-PDMAA hydrogel has potential for application as an artificial tissue.
Calcium phosphate (CaP) has been widely used as the vector for gene transfection in the past three decades. However, clinical application is still not popular due to the poor-controlling of DNA/CaP ...complexes preparation, cytotoxicity and its low transfection efficiency. In this study, a novel amphipathic octadecyl-quatemized carboxymethyl chitosan (OQCMC) derivative from chitosan was combined with calcium phosphate to synthesize CaP/OQCMC nanoparticles (CaP/OQCMC NPs). The nanoparticles were 122-177 nm in diameter exhibited neutral zeta potential (from -0.115 mV to 0.216 mV), and they were applied as DNA vectors for DNA loading and in vitro transfection. The results showed that CaP/OQCMC NPs displayed high DNA loading capacity and enhanced transfection efficiency with extremely low cytotoxicity. In addition, both CaP and OQCMC are biocompatible and biodegradable, thus the as-prepared CaP/OQCMC NPs are promising in gene delivery.
This study aims to determine the sensitivity, specificity and accuracy of epidermal growth factor receptor monoclonal antibody (EGFRmAb) modified poly(lactic acid-
co
-
l
-lysine) nanoparticles ...(PLA-PLL-EGFRmAb) NPs delivery system to EGFR positive cancer cells. In the study, a new PLA-PLL-EGFRmAb NPs was prepared. The cellular cytotoxicity, cellular uptake, and the targeted effect for hepatocellular carcinoma of PLA-PLL-EGFRmAb NPs were investigated. In vitro, the findings of Flow cytometry and Confocal Laser scanning Biological Microscopy showed that PLA-PLL-EGFRmAb NPs can bind to hepatocellular carcinoma cells and were uptaken effectively. In vivo in the SMMC-7721 xenograft mouse model, PLA-PLL-EGFRmAb NPs could target to the tumor effectively, which demonstrated a better targeting. These results showed that the PLA-PLL-EGFRmAb NPs have the potential to be used as a target delivery carrier for tumor therapies.
Study of SiRNA-loaded PS-mPEG/CaP nanospheres on lung cancer Wang, Qi; Qin, Liubin; Sun, Ying ...
Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology,
05/2014, Letnik:
16, Številka:
5
Journal Article
An ultrasound-adsorption method was used to prepare Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres. The size and zeta potential were 18.41 ± 4.31 nm (
n
= 5) and −23.5 ± 0.6 mV, respectively. The ...entrapment efficiency of SiRNA was 92.86 %. MTT assay results confirmed that the blank nanospheres demonstrated a negligible cytotoxicity response in H1299 cells. Flow cytometer analysis results demonstrated that PS-mPEG/CaP NSs could carry SiRNA into the cells effectively. RT-PCR experiments and apoptosis assay results approved that, compared with free SiRNA, SiRNA-loaded PS-mPEG/CaP NSs could silence Bcl-2 gene and induce cell apoptosis effectively. In vivo distribution results confirmed PS-mPEG/CaP NSs could carry SiRNA enter the tumor tissue effectively. Taken together, these results suggest that the Bcl-2-SiRNA-loaded PS-mPEG/CaP nanospheres have great potential to be used to cure lung cancer.
Drug-phospholipid lipid nanoparticles (DPLNs) can effectively enhance the properties of traditional solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs), as previously ...demonstrated by our research group and others. To date, however, very few studies have focused on the cellular uptake mechanism and fate of DPLNs in hepatoma. Therefore, we systematically studied the cellular uptake mechanism and endosomal fate of DPLNs through in vitro and in vivo experiments. Confocal laser scanning microscopy (CLSM) and flow cytometry demonstrated that the Raw264.7 cell line (macrophage Raw264.7 cells), Chang cells (a human liver cell line) and HepG2 cells (a human hepatoma cell line) exhibited distinct uptake mechanisms. The Raw264.7 cells served as a model for examining liver-targeting ability. The results from mice with subcutaneous hepatomas and in situ hepatomas confirmed that the liver tumor-targeting property of the DPLNs was associated with the liver drug reservoir function. These findings further improve our understanding of DPLNs for clinical applications.
The combination of ultrasound (US) and microbubbles (MB) is a promising physical method for improving the nanoparticles (NPs) delivery efficiency. However, few concerns over comparable delivery ...effect of the passive or active targeting property's NPs mediated by US and MB have limited their translation towards further application. For this, we prepared small interfering RNA (siRNA)-loaded mPEG-PLGA-PLL (siRNA/mPPP) NPs with passive targeting property and siRNA-loaded mPEG-PLGA-PLL-cRGD (siRNA/mPPPR) NPs with active targeting property, and evaluated the effect of US and MB for their delivery efficiency. The experimental results demonstrated that US and MB effectively enhance the siRNA delivery efficiency of the mPPP NPs compared with the mPPP NPs alone. In contrast, an improved delivery efficiency of siRNA was not observed in PC-3 cells treated with the mPPPR NPs mediated by US and MB, suggesting that the delivery efficiency of NPs mediated US and MB also depend on its targeting properties.
Abstract A amphiphilic block copolymer composed of conventional monomethoxy (polyethylene glycol)-poly ( d , l -lactide- co -glycolide)-poly ( l -lysine) (mPEG-PLGA- b -PLL) was synthesized. The ...chemical structure of this copolymer and its precursors was confirmed by Fourier Transform Infrared Spectroscopy (FTIR),1 H Nuclear Magnetic Resonance (1 H NMR) and Gel Permeation Chromatography (GPC). The copolymer was used to prepare nanoparticles (NPs) that were then loaded with either the anti-cancer drug adriamycin or small interfering RNA-negative (siRNA) using a double emulsion method. MTT assays used to study the in vitro cytotoxicity of mPEG-PLGA- b -PLL NPs showed that these particles were not toxic in huh-7 hepatic carcinoma cells. Confocal laser scanning microscopy (CLSM) and flow cytometer analysis results demonstrated efficient mPEG-PLGA- b -PLL NPs-mediated delivery of both adriamycin and siRNA into the cells. In vivo the targeting delivery of adriamycin or siRNA mediated by mPEG-PLGA- b -PLL NPs in the huh-7 hepatic carcinoma-bearing mice was evaluated using a fluorescence imaging system. The targeting delivery results and froze section analysis confirmed that drug or siRNA is deliver to tumor more efficiently by mPEG-PLGA- b -PLL NPs than free drug or Lipofectamine™2000. The high efficiency delivery of mPEG-PLGA- b -PLL NPs mainly due to the enhancement of cellular uptake. These results imply that mPEG-PLGA- b -PLL NPs have a great potential to be used as an effective carriers for adriamycin or siRNA.
This study describes the preparation and the evaluation of biodegradation monomethoxy (polyethylene glycol)-poly (lactide-co-glycolide)-monomethoxy (polyethyleneglycol) (mPEG-PLGA-mPEG, PELGE) ...nanoparticles (PELGE-NP) containing mitoxantrone (DHAQ) as a model drug. PELGE copolymers with various molar ratios of lactic to glycolic acid and different molecular weights and various content mPEG were synthesized by ring-opening polymerization. mPEG with weight-average molecular weight (Mw) 2,000 or 5,000 was introduced as a hydrophilic segment into a hydrophobic PLGA. A double emulsion method with dextran70 as stabilizer in the external aqueous phase was used to prepare the nanoparticles. The drug entrapment efficiencies were more than 80% and the mean diameters of the nanoparticles were less than 200 nm. Various PELGE was studied as biodegradable drug carriers and there in vitro/in vivo release profiles were examined. It was found that drug loading, polymer molecular weight, copolymer composition and end group modifications were critical factors affecting the in vitro/in vivo release properties. The amount of drug released increased as the mPEG contents increased and the molar ratios of lactic acid decreased in vitro. The intravenous (i.v.) administration of mPEG-PLGA-mPEG nanoparticles of DHAQ in mice resulted in prolonged DHAQ residence in systemic blood circulation compared to the intravenous administration of PLGA nanoparticles.
Triptolide has been proven to possess anticancer efficacy; however, its application in the clinical practice was limited by poor water solubility, hepatotoxicity, and nephrotoxicity. In this study, a ...triptolide-loaded exosomes delivery system (TP-Exos) was constructed and its effects on the proliferation and apoptosis of SKOV3 cells in vitro and in vivo were observed. SKOV3-exosomes (SK-Exos) were collected by ultracentrifugation and ultrafiltration centrifugation. TP-Exos was constructed by sonication and ultrafiltration centrifugation. SK-Exos and TP-Exos were characterized by transmission electron microscopy, western blotting, nanoparticle-tracking analysis, and high-performance liquid chromatography. Cellular uptake of exosomes, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine (BrdU) cell proliferation assay, and cell apoptosis experiment were used to study the effect of TP-Exos on ovarian cancer in vitro. Tumor-targeting study of exosomes, monitoring the tumor volume of mice, and TdT-mediated dUTP Nick-End labeling (TUNEL) assay were used to evaluate the effect of TP-Exos on ovarian cancer in vivo. The toxicity of TP-Exos in vivo was evaluated by liver and kidney function and histopathology of major organs (heart, liver, spleen, lung, kidney, and ovary). The results revealed that TP-Exos not only have the general characteristics of exosomes but also have high drug encapsulation efficiency. Besides, PKH26 labeled exosomes (PKH26-Exos) could be uptaken by SKOV3 cells, and Dir labeled exosomes (Dir-Exos) could be enriched to the tumor site of tumor bearing mice. Furthermore, the cytotoxic and apoptotic effects on SKOV3 cells of TP-Exos were weaker than those of free TP, and tumor cell proliferation inhibition and tumor growth inhibition were stronger than that of free TP. Moreover, TP-Exos have toxic effect on liver and spleen. In conclusion, the TP-Exos could be a promising strategy for ovarian cancer, but they need to be further optimized to attenuate the damage to liver and spleen.
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