Previous research has reported diverging patterns of results with respect to discriminability and response bias when comparing the simultaneous lineup to two different lineup procedures in which ...items are presented sequentially, the sequential stopping rule lineup and the UK lineup. In a single large sample experiment, we compared discriminability and response bias in six-item photographic lineups presented either simultaneously, sequentially with a stopping rule, or sequentially requiring two full laps through the items before making an identification and including the ability to revisit items, analogous to the UK lineup procedure. Discriminability was greater for the simultaneous lineup compared to the sequential stopping rule lineup, despite a non-significant difference in empirical discriminability between the procedures. There was no significant difference in discriminability when comparing the simultaneous lineup to the sequential two lineup and the sequential two lap lineup to the sequential stopping rule lineup. Responding was most lenient for the sequential two lap lineup, followed by the simultaneous lineup, followed by the sequential lineup. These results imply that sequential item presentation may not exert a large effect in isolation on discriminability and response bias. Rather, discriminability and response bias in the sequential stopping rule lineup and UK lineup result from the interaction of sequential item presentation with other aspects of these procedures.
•First published study to compare a UK-style lineup (a sequential two-lap lineup) to a sequential stopping rule lineup.•Discriminability was greater for the simultaneous lineup compared to the sequential stopping rule lineup.•Responding was most lenient in the sequential two lap lineup and most conservative in the sequential stopping rule lineup.•Results partially support the Diagnostic Feature Detection Theory of eyewitness memory.
Laboratory-based mock crime studies have often been interpreted to mean that (i) eyewitness confidence in an identification made from a lineup is a weak indicator of accuracy and (ii) sequential ...lineups are diagnostically superior to traditional simultaneous lineups. Largely as a result, juries are increasingly encouraged to disregard eyewitness confidence, and up to 30% of law enforcement agencies in the United States have adopted the sequential procedure. We conducted a field study of actual eyewitnesses who were assigned to simultaneous or sequential photo lineups in the Houston Police Department over a 1-y period. Identifications were made using a three-point confidence scale, and a signal detection model was used to analyze and interpret the results. Our findings suggest that (i) confidence in an eyewitness identification from a fair lineup is a highly reliable indicator of accuracy and (ii) if there is any difference in diagnostic accuracy between the two lineup formats, it likely favors the simultaneous procedure.
Signal detection theory (SDT) plays a central role in the characterization of human judgments in a wide range of domains, most prominently in recognition memory. But despite its success, many of its ...fundamental properties are often misunderstood, especially when it comes to its testability. The present work examines five main properties that are characteristic of existing SDT models of recognition memory: (a) random-scale representation, (b) latent-variable independence, (c) likelihood-ratio monotonicity, (d) ROC function asymmetry, and (e) nonthreshold representation. In each case, we establish testable consequences and test them against data collected in the appropriately designed recognition-memory experiment. We also discuss the connection between yes-no, forced-choice, and ranking judgments. This connection introduces additional behavioral constraints and yields an alternative method of reconstructing yes-no ROC functions. Overall, the reported results provide a strong empirical foundation for SDT modeling in recognition memory.
This article addresses the issue of whether the remember-know (RK) task is best explained by a single-process or a dual-process model. All single-process models propose that remember and know ...responses reflect different levels of a single strength-of-evidence dimension. Thus, across conditions in which response criteria are held constant, these models predict that the RK task is unidimensional. Many dual-process models propose that remember and know responses reflect two qualitatively distinct processes underlying recognition memory, often characterized as recollection and familiarity. These models predict that the RK task is bidimensional. Using data from 37 studies, the author conducted a state-trace analysis to determine the dimensionality of the RK task. In those studies, non-memory-related differences between conditions were eliminated via decision criteria constrained to be constant across all levels of the independent variables. The results reveal little or no evidence of bidimensionality and lend additional support to the unequal-variance signal detection model. Other arguments supporting a bidimensional interpretation are examined, and the author concludes there is insufficient evidence for the RK task to be used to identify qualitatively different memory components.
Polymerases that synthesize artificial genetic polymers hold great promise for advancing future applications in synthetic biology. However, engineering natural polymerases to replicate unnatural ...genetic polymers is a challenging problem. Here we present droplet-based optical polymerase sorting (DrOPS) as a general strategy for expanding polymerase function that employs an optical sensor to monitor polymerase activity inside the microenvironment of a uniform synthetic compartment generated by microfluidics. We validated this approach by performing a complete cycle of encapsulation, sorting and recovery on a doped library and observed an enrichment of ∼1,200-fold for a model engineered polymerase. We then applied our method to evolve a manganese-independent α-L-threofuranosyl nucleic acid (TNA) polymerase that functions with >99% template-copying fidelity. Based on our findings, we suggest that DrOPS is a versatile tool that could be used to evolve any polymerase function, where optical detection can be achieved by Watson-Crick base pairing.
Remember-Know Dunn, John C
Psychological review,
04/2004, Letnik:
111, Številka:
2
Journal Article
Recenzirano
This article critically examines the view that the signal detection theory (SDT) interpretation of the remember-know (RK) paradigm has been ruled out by the evidence. The author evaluates 5 empirical ...arguments against a database of 72 studies reporting RK data under 400 different conditions. These arguments concern (a) the functional independence of remember and know rates, (b) the invariance of estimates of sensitivity, (c) the relationship between remember rates and overall hit and false alarm rates, (d) the relationship between RK responses and confidence judgments, and (e) dissociations between remember and overall hit rates. Each of these arguments is shown to be flawed, and despite being open to refutation, the SDT interpretation is consistent with existing data from both the RK and remember-know-guess paradigms and offers a basis for further theoretical development.
Aptamers are often prone to nuclease digestion, which limits their utility in many biomedical applications. Here we describe a xeno-nucleic acid system based on α-l-threofuranosyl nucleic acid (TNA) ...that is completely refractory to nuclease digestion. The use of an engineered TNA polymerase permitted the isolation of functional TNA aptamers that bind to HIV reverse transcriptase (HIV RT) with K D’s of ∼0.4–4.0 nM. The aptamers were identified using a display strategy that provides a powerful genotype–phenotype linkage. The TNA aptamers remain active in the presence of nuclease and exhibit markedly higher thermal stability than monoclonal antibodies. The combined properties of biological stability, high binding affinity, and thermal stability make TNA aptamers a powerful system for the development of diagnostic and therapeutic agents.
Synthetic genetics is a subdiscipline of synthetic biology that aims to develop artificial genetic polymers (also referred to as xeno-nucleic acids or XNAs) that can replicate in vitro and eventually ...in model cellular organisms. This field of science combines organic chemistry with polymerase engineering to create alternative forms of DNA that can store genetic information and evolve in response to external stimuli. Practitioners of synthetic genetics postulate that XNA could be used to safeguard synthetic biology organisms by storing genetic information in orthogonal chromosomes. XNA polymers are also under active investigation as a source of nuclease resistant affinity reagents (aptamers) and catalysts (xenozymes) with practical applications in disease diagnosis and treatment. In this review, we provide a structural perspective on known antiparallel duplex structures in which at least one strand of the Watson-Crick duplex is composed entirely of XNA. Currently, only a handful of XNA structures have been archived in the Protein Data Bank as compared to the more than 100 000 structures that are now available. Given the growing interest in xenobiology projects, we chose to compare the structural features of XNA polymers and discuss their potential to access new regions of nucleic acid fold space.
Hip and knee arthroplasties length of stay continues to shorten after advances in perioperative and intraoperative management, as well as financial incentives. Some authors have demonstrated good ...results with outpatient arthroplasty, but safety and general feasibility of such procedures remain unclear. Our hypothesis is that outpatient arthroplasty would demonstrate higher readmission and complication rates than inpatient arthroplasty.
We performed a systematic review of all publications on outpatient arthroplasty between January 1, 2000 and June 1, 2016. Included publications had to demonstrate a specific outpatient protocol and have reported perioperative complications and unplanned readmissions. Patient demographics, surgical variables, and protocol details were recorded in addition to complications, readmission, and reoperation.
Ten manuscripts accounting for 1009 patients demonstrated that 955 (94.7%) were discharged the same day as planned, with the majority of failures to discharge being secondary to pain, hypotension, and nausea. There were no deaths and only 1 major complication. Only 20 patients (1.98%) required reoperation and 20 (1.98%) had readmission or visited the emergency room within 90 days of their operation. In the 2 series recording patient outcomes, 80% and 96% of patients reported that they would choose to undergo outpatient arthroplasty again.
For carefully selected patients with experienced surgeons in major centers, outpatient arthroplasty may be a safe and effective procedure. Although our data is promising, further study is required to better elucidate the differences between inpatient and outpatient arthroplasty outcomes.
Abstract Background This investigation sought to quantify incidence rates (IRs) and risk factors for primary and secondary (ie, posttraumatic) osteoarthritis (OA) of the knee in an active military ...population. Methods We performed a retrospective review of United States military active duty servicemembers with first-time diagnosis of primary (International Classification of Disease, 9th Edition code: 715.16) and secondary (International Classification of Disease, 9th Edition code: 715.26) OA of the knee between 2005 and 2014 using the Defense Medical Epidemiology Database. IRs and 95% CIs were expressed per 1000 person-years, with stratified subgroup analysis adjusted for sex, age, race, military rank, and branch of military service. Relative risk factors were evaluated using IR ratios and multiple regression analysis. Results A total of 21,318 cases of OA of the knee were identified among an at-risk population of 13,820,906 person-years for an overall IR of 1.54 per 1000 person-years, including 19,504 cases of primary (IR: 1.41) and 1814 cases of secondary OA (IR: 0.13). The IRs of both primary and secondary OA increased significantly from 2005 to 2014. Increasing age ( P < .0001); black race ( P < .001); senior military rank ( P < .0001); and Army, Marines, and Air Force services ( P < .0001) were significantly associated with an increased risk for knee OA. Conclusion This study is the first large-scale report of knee OA in a young athletic population. An increasing incidence and several risk factors for knee OA were identified, indicating a need for better preventative strategies and forecasting the increased anticipated demands for knee arthroplasty among US military servicemembers.
PDF
