The pre‐cervical uterine torsion is a rotation of a pregnant uterine horn on its longitudinal axis with the point of rotation located cranial to the cervix. After diagnosis based on vaginal and ...rectal examination, uterine detorsion was first transrectal approached because the cervix was not open and blocked. Five cases are described. The direction of rotation in all 5 cows was clockwise. The head of the calf was manually caught with the left hand per rectum and the entire uterus swayed several times in a left–right direction. During left–right swinging of the pregnant uterus and fetus, manually through the rectum, when the right swing tip reached, then abruptly tried to turn anti‐clockwise. The procedure was repeated several times. After partial transrectal detorsion, we could access the calf, vaginally, and detorsion with the right hand and the same procedure of swinging the calf were started, but vaginally. Even if the uterus was restored to the normal position, sometimes the calf remained in an unfavourable position; therefore, it was corrected in the dorsal position and prepared for a forced extraction. This is the first report of a successful combination of transrectal and vaginal manual detorsion of a rare pre‐cervical uterine torsion in cows.
•Prophylactic and therapeutic use of ozone in buiatrics.•Treatment of reproductive tract and mammary gland in ruminants by ozone.•Potentials and limitations of ozone use in veterinary medicine.
Ozone ...therapy has been in use since 1896 in the USA. As a highly reactive molecule, ozone may inactivate bacteria, viruses, fungi, yeasts and protozoans, stimulate the oxygen metabolism of tissue, treat diseases, activate the immune system, and exhibit strong analgesic activity. More recently, ozone has been used in veterinary medicine, particularly in buiatrics, but still insufficiently. Medical ozone therapy has shown effectiveness as an alternative to the use of antibiotics, which are restricted to clinical use and have been withdrawn from non-clinical use as in-feed growth promoters in animal production. This review is an overview of current knowledge regarding the preventive and therapeutic effects of ozone in ruminants for the treatment of puerperal diseases and improvement in their fertility. In particular, ozone preparations have been tested in the treatment of reproductive tract lesions, urovagina and pneumomovagina, metritis, endometritis, fetal membrane retention and mastitis, as well as in the functional restoration of endometrium in dairy cows and goats. In addition, the preventive use of the intrauterine application of ozone has been assessed in order to evaluate its effectiveness in improving reproductive efficiency in dairy cows. No adverse effects were observed in cows and goats treated with ozone preparations. Moreover, there is a lot of evidence indicating the advantages of ozone preparation therapy in comparison to the application of antibiotics. However, there are certain limitations on ozone use in veterinary medicine and buiatrics, such as inactivity against intracellular microbes and selective activity against the same bacterial species, as well as the induction of tissue inflammation through inappropriate application of the preparation.
The bacterial species
is the most common causative agent of mastitis in cows in most countries with a dairy industry. The prevalence of infection caused by
ranges from 2% to more than 50%, and it ...causes 10-12% of all cases of clinical mastitis.
The objective was to analyze 237 strains of
isolated from the milk of cows with subclinical mastitis regarding the
,
and
genes and to perform
and multi-locus sequence typing (MLST).
Sequencing amplified gene sequences was conducted at Macrogen Europe. Ridom StaphType and BioNumerics software was used to analyze obtained sequences of
and seven housekeeping genes.
The
fragment was present in 204 (86.1%) of strains, while
and
gene were detected in 10 strains, and the
gene was not detected.
typing successfully analyzed 153 tested isolates (64.3%), confirming 53
types, four of which were new types. The most frequent
type was t2678 (14%). MLST typed 198 (83.5%) tested strains and defined 32 different allele profiles, of which three were new. The most frequent allele profile was ST133 (20.7%). Six groups (G) and 15 singletons were defined.
Taking the number of confirmed
types and sequence types (STs) into account, it can be concluded that the strains of
isolated from the milk of cows with subclinical mastitis form a heterogenous group. To check the possible zoonotic potential of isolates it would be necessary to test the persons and other livestock on the farms.
The aim of the study was to determine effects of dietary clinoptilolite (CPL) supplementation on β-hydroxybutirate (BHB) serum levels and milk fat to protein ratio (FPR) during early lactation in ...Holstein-Friesian cows. Seventy cows of the Holstein-Friesian breed, aged from 3 to 5 years, kept in a farm nearby Đurđevac, Croatia, were used in the study. The cows were randomly assigned into two groups, either test, in-feed CPL treated; CPL (n = 35) or control, non-treated group; CON (n = 35). The CPL group received 50 g of natural modified CPL preparation in the ratio for dairy cows, twice a day, from third month of pregnancy. Blood and milk samples were collected from the cows after parturition on days 7, 12, 19, 26 and 33 of lactation. The average value of BHB was higher in the CON than in the CPL group (1.03 ± 0.78 vs. 0.82 ± 0.42 mmol/L), as well as average value of FPR (1.32 ± 0.20 vs. 1.24 ± 0.14), but without significant differences. The highest BHB serum levels were obtained on days 12 and 19 of lactation in the CON group and in the CPL group on day 12. The cows in CPL group had lower BHB levels and FPR ratio, mostly, in the optimal range or below, while cows from CON group had FPR higher than optimal on days 19, 26 and 33 after parturition. In the CON and CPL groups 17.5% and 7.9% cows, respectively suffered from subclinical ketosis. On days 7, 12 and 19 non-esterified fatty acids were significantly higher in the CON group than in the CPL group. The cows from the CON group were in severe and longer negative energy balance than the cows from the CPL group. In conclusion, the CPL had positive effects on BHB serum levels and FPR during early lactation in Holstein-Friesian cows.
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•Zeolite decreased BHB serum level during early lactation in dairy cows.•Zeolite had positive effect on milk fat to protein ratio during early lactation in cows.•Zeolite decreased severity and duration of negative energy balance in dairy cows.
Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production.
To investigate the difference in the serum proteome between the ...cows with RP and the physiologic puerperium (PP).
Analysis of serum samples from nine cows with RP and six with PP using high-resolution liquid chromatography-tandem mass spectrometry approach. The proteins differing in the relative abundance between the PP and RP groups were classified using the Protein Analysis Through Evolutionary Relationship tool. For the pathway enrichment analysis, the REACTOME tool, with the human genome as the background, was employed. The criterion for significance was the false discovery rate corrected P-value less than 0.05.
In total 651 proteins were identified with altered relative abundance of ten proteins. Among them, seven had higher, and three showed lower relative abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in lipoprotein metabolism, and the remaining ones associated with for instance redox homeostasis, post-translational modification, and scavenging. Finally, the validation of the proteomic results showed that haptoglobin and lipopolysaccharide-binding protein levels reliably differentiated between the RP and PP groups.
The pattern of serum proteome alterations in the cows with RP mirrored several interplaying mechanisms underlying the systematic response to the presence of RP, therefore representing a source to mine for predictive or prognostic biomarkers.
The aim of this study was to investigate the influence of season, rainfall and air temperature on the reproductive efficiency in the Romanov breed of sheep in continental part of Croatia during five ...consecutive years (2012–2016). During this period, 5379 matings resulted in 5046 successful conceptions, i.e. lambings at eight medium-scale Romanov breed sheep farms. The conception rate was 93.81%, fecundity was 195% and average preweaning mortality until 90 days of age was 12.41%. The seasonal distribution of lambings was 47.64% for ewes that delivered in winter (
n
= 2422), 23.37% in spring (
n
= 1179), 18.82% in summer (
n
= 950) and 9.81% in autumn (
n
= 495). Sexual activity was lowest during spring and early summer when air temperatures were above average (very and extremely warm), and sexual activity peaked from August to September, especially during extremely wet and very wet seasons. Litter size was greater during winter than in other seasons (1.70 vs. 1.54) and was significantly different as compared to each of selected years of the study period. There was a statistically significant difference in the number of pregnant ewes between mating seasons. Most female Romanov lambs born during winter and early spring mated in late summer or autumn and delivered at the age of 1 year or earlier. The seasonal distribution of matings and lambing was not uniform throughout the seasons over five consecutive years. Thus, it can be assumed that air temperature and rainfall during different seasons could affect the reproductive efficiency in Romanov breed of sheep in continental part of Croatia.
This study aimed to determine the impact of relocation as a potential stress factor on the reproductive properties of breeding boars, as well as the impact of the new environment on the quantity and ...quality of the semen obtained during different seasons. At the beginning of spring, 16 breeding boars (Large White (n=3), German Landrace (n=4), Swedish Landrace (n=6) and Pietrain (n=3)) were relocated from old to new housing conditions and exposed to loading, transport, unloading, and rehousing in a new environment. They had the same conditions of feeding, care, sexual exploitation and keeping, before and after relocation. The health status of all breeding boars was carefully monitored and controlled. No significant differences were observed in the total and average number of doses produced between seasons. There was a significantly higher value of total ejaculate volume and density during the spring compared to the values obtained in other seasons (P<0.01). Differences in the average percentage of mobility between autumn and winter were not significant (P=0.983), as in spring and summer (P=0.737). Ventilation rate, gas concentrations of ammonia, nitrous oxide and carbon dioxide in the boar enclosure were similar before and after relocation. Two days after relocation, one boar (ID 172) showed signs of illness and was treated, but his sperm was rejected due to sperm agglutination and reduced motility, so he was culled. Individual analysis of the production indicators showed that there was no significant change in the spermatogenesis of healthy boars, even though they were relocated.
The enzyme concentrations of seminal plasma are important for spermatozoa metabolism and function in boars. The need has arisen for introducing a biochemical evaluation of semen, along with the usual ...standard semen analyses. There are no data on the influence of boar breeds on the seminal plasma biochemical variables investigated in this study. Therefore, the objective was to determine the influence of breed and hybrid genetic composition of boars on semen quality and seminal plasma biochemical variables. Semen samples of 27 boars (Swedish Landrace, German Landrace, Large White, Pietrain and Pig Improvement Company hybrid—PIC-hybrid), aged between 1.5 and 3 years, were collected. After evaluation of semen quality, the seminal plasma was separated from the spermatozoa by centrifugation of semen. The seminal plasma was subjected to spectrophotometric analysis to determine alkaline phosphatase (ALP), acid phosphatase (ACP), γ-glutamyltransferase (GGT), creatine kinase (CK) and lactate dehydrogenase (LDH) and to atomic absorption spectrophotometric analysis to measure the concentration of calcium and magnesium. Conventional semen quality variables differed depending on breed and PIC-hybrid genetic composition, though these differences were typically insignificant. In the seminal plasma, significant differences were determined in enzyme activity (ALP, GGT, CK and LDH) and in calcium concentration among boars of differnt breeds. There are, therefore, differences in semen quality and significant differences in the seminal plasma biochemical variables among boars of different breeds and PIC-hybrid genetic composition. The data and differences in semen variables detected in the present study provide knowledge for enhancing evaluation and monitoring of boar reproductive potential, semen quality and explain the potential causes of boar infertility.
The objectives of this study were to determine the influence of breed and hybrid genetic traits of boars on lipid and protein concentrations and antioxidative system variables in seminal plasma (SP) ...and spermatozoa and their correlations with semen quality variables. Semen samples from 27 boars: Swedish Landraces (SL), German Landraces (GL), Large Whites (LW), Pietrains (P) and Pig Improvement Company hybrids (PIC-hybrid), aged from 1.5 to 3 years old, were collected. SP was spectrophotometrically analyzed to determine total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triacylglycerol (TAG), total protein (TP), albumin, and zinc concentrations. The antioxidative system in SP and spermatozoa was established spectrophotometrically by determining total antioxidative status (TAS), total superoxide dismutase (TSOD) and glutathione peroxidase (GSH-Px) parameters, as well as copper-zinc superoxide dismutase (CuZnSOD) and manganese superoxide dismutase (MnSOD) activity in spermatozoa. The hybrid boars had higher (P<0.05) SP concentrations of: TC, LDL-C and TAG than P and GL; HDL-C than P, GL and SL; and TP than P and LW. PIC-hybrid had lower values (P<0.05) in spermatozoa of: TAS and CuZnSOD than SL; TSOD and GSH-Px than SL and P; and MnSOD than SL and LW. Differences in SP and spermatozoa antioxidative system variables and the significant differences in SP protein and lipid variables exist among boars of different breeds and hybrid. Novel data and observed differences in semen variables among boar breeds and hybrids and their correlations with semen quality parameters in this study could contribute to better assessment of boar semen quality.
Dairy cows can suffer from a negative energy balance (NEB) during their transition from the dry period to early lactation, which can increase the risk of postpartum diseases such as clinical ketosis, ...mastitis, and fatty liver. Zeolite clinoptilolite (CPL), due to its ion-exchange property, has often been used to treat NEB in animals. However, limited information is available on the dynamics of global metabolomics and proteomic profiles in serum that could provide a better understanding of the associated altered biological pathways in response to CPL. Thus, in the present study, a total 64 serum samples were collected from 8 control and 8 CPL-treated cows at different time points in the prepartum and postpartum stages. Labelled proteomics and untargeted metabolomics resulted in identification of 64 and 21 differentially expressed proteins and metabolites, respectively, which appear to play key roles in restoring energy balance (EB) after CPL supplementation. Joint pathway and interaction analysis revealed cross-talks among valproic acid, leucic acid, glycerol, fibronectin, and kinninogen-1, which could be responsible for restoring NEB. By using a global proteomics and metabolomics strategy, the present study concluded that CPL supplementation could lower NEB in just a few weeks, and explained the possible underlying pathways employed by CPL.
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