This study aimed to determine the impact of relocation as a potential stress factor on the reproductive properties of breeding boars, as well as the impact of the new environment on the quantity and ...quality of the semen obtained during different seasons. At the beginning of spring, 16 breeding boars (Large White (n=3), German Landrace (n=4), Swedish Landrace (n=6) and Pietrain (n=3)) were relocated from old to new housing conditions and exposed to loading, transport, unloading, and rehousing in a new environment. They had the same conditions of feeding, care, sexual exploitation and keeping, before and after relocation. The health status of all breeding boars was carefully monitored and controlled. No significant differences were observed in the total and average number of doses produced between seasons. There was a significantly higher value of total ejaculate volume and density during the spring compared to the values obtained in other seasons (P<0.01). Differences in the average percentage of mobility between autumn and winter were not significant (P=0.983), as in spring and summer (P=0.737). Ventilation rate, gas concentrations of ammonia, nitrous oxide and carbon dioxide in the boar enclosure were similar before and after relocation. Two days after relocation, one boar (ID 172) showed signs of illness and was treated, but his sperm was rejected due to sperm agglutination and reduced motility, so he was culled. Individual analysis of the production indicators showed that there was no significant change in the spermatogenesis of healthy boars, even though they were relocated.
Radiation processing of fish is recognized as a safe and effective method for reducing microorganisms and viruses as well for inactivating pathogens among the existing technologies for preservation. ...In the present study, the effect of gamma irradiation and temperature of storage on the histamine production, lipid peroxidation and antioxidant parameters in sardine (Sardina pilchardus, Walbaum, 1792) were investigated. Fish samples were irradiated with different doses (0 kGy, 1 kGy and 3 kGy) and monitored during the storage at two different temperatures (4 °C and 30 °C). The results indicate that histamine concentration was reduced by gamma irradiation and that the safe consumption can be prolongated for both temperatures of storage. However, irradiation treatment induced oxidative damage, as evidenced by changes in levels of lipid peroxidation, activity of the antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) as well as in the radical kinetic rate detected by EPR (electron paramagnetic resonance) spectroscopy. These results suggest that gamma radiation undoubtedly induces antioxidant defense system in sardine fish. However, further research is necessary to elucidate the precise role that the antioxidant system plays under the influence of gamma radiation and temperature.
•Antioxidant defense in γ-irradiated Sardine during storage at 4 °C and 30 °C.•γ-irradiation reducing histamine contents in Sardine.•Influence of different temperatures on the reduction of the histamine.
Determining the levels of biogenic amines in cheese except that it has significance for determining the nutritional value and hygienic accuracy, cheese as food can be used as a parameter to evaluate ...the conditions of production and/or ripening of products, and particularly in the selection of bacterial cultures. The purpose of this paper was to determine the effect of the ripening process on the amount of biogenic amines in cheese. For this purpose were conducted physico-chemical analysis, determination of biogenic amines and microbiological analysis. During the process of ripening Livno cheese from three different batches was taken one cheese from prime day and 9th, 20th, 29th, 50th, 60th and 105th day. From each cheese two samples were taken, one from the middle and one from the cheese rind. During 105th day of ripening Livno cheese, the presence of triptamin, ß-feniletlamine, putrescine, cadaverine, histamine, tyramine, spermine and spermidine were determinated. The maximum total values of searched biogenic amines were found 105th day, in the middle 184.13 mg/kg and 76.26 mg/kg in the rind of cheese. With an indication that the largest share of value rep¬resent histamine with 43.9 % and tyramine with 38.2 % in the middle, respectively histamine with 31.6 % and tyramine with 31.5 % in the rind of cheese. The values of putrescine and spermine were in small ranges and they are not identified in all samples. The values of histamine and tyramine are almost a third more at 105th than 60th day. There was a significant difference between the middle and the rind of cheese in the values of biogenic amines. Correlation between biogenic amines and microorganisms has not been determined.
The Postmortem Interval (PMI) is the time from the death of an animal to its discovery. From a veterinary forensic standpoint, an accurate estimation of the PMI is of particular importance, ...especially with the observed increase in deaths of domestic and wild animals. A preliminary study was conducted using the eyes of domestic pigs. A biochemical analysis was conducted on the vitreous humor of the eye, whilst a histological analysis was conducted on the retina. The eyes were stored at +4 °C and changes were assessed at time intervals of 0, 12, 24, 48, and 120 h. The biochemical analysis during the PMI established a decrease in sodium, chlorine, and glucose concentrations, and a rise in potassium concentration. Accordingly, a simple linear regression showed a significant correlation between changes in concentrations of sodium (Na
), potassium (K
), chloride (Cl
), and glucose, in relation to the PMI. The histological analysis showed evident morphological changes in the retina, which included homogenization of the rod and cone cells, pyknosis of the outer nuclear layer, homogenization of the outer plexiform layer, pyknosis of the inner nuclear layer, homogenization of the inner plexiform layer, and pyknosis of the nuclei of the ganglion layer of the retina.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
SEVERIN, K, P. DZAJA, M. SPERANDA, M. DIDARA, D. KONJEVIC, E. SATROVIC, K STARCEVIC: Estimation of red deer (Cervus elaphus) post mortal interval based on the biochemical parameters of vitreous fluid ...using linear regression analyses. Vet. arhiv 88, 511-519, 2018. Vitreous fluid is a stable biological fluid, which is well-protected from contamination and post mortem degradation, which makes it suitable in forensic pathology for determination of the post mortem interval (PMI). The present study was conducted to discover the correlation between increasing PMI and levels of various vitreous biochemical parameters (sodium, calcium, magnesium, chloride, potassium, phosphor, AST, urea, creatinine, total protein, glucose, total cholesterol). The estimation of PMI in red deer is of great interest in cases of illegal hunting. The vitreous humour samples were collected from six red deer carcasses. The eyes were enucleated, covered by parafilm and aluminium foil, packed in a small container and maintained at +4 degreesC. Sampling of vitreous fluid was done again at 8, 10 and then regularly each 10 hours until finally 90 hours after death. A significant linear correlation was found for potassium, sodium and chloride (P<0.0001). The present study revealed that biochemical analysis of vitreous fluid could be used to determine post mortem interval in red deer. Key words: vitreous fluid; potassium; sodium; chloride; red deer
An epidemiological study was carried out to determine the seroprevalence of Chlamydia abortus (C. abortus) in sheep in Bosnia and Herzegovina. This was the first systematic study of this kind carried ...out in Bosnia and Herzegovina. Samples were collected during 2012 and 19 sheep flocks, located in the different parts of the country and with a recorded history of reproductive failures (abortion, stillbirths and infertility) were involved. A representative sample from each flock was taken by a simple random sampling allowing the detection of seropositive animals within a flock with 95% confidence, with expected prevalence rates of 20%, using the recommendations for determining the required necessary sample size to detect the presence of disease. In total 178 sheep blood sera were tested for the specific antibodies against C. abortus, with the use of enzyme-linked immunosorbent assay (CHEKIT* Chlamydophila abortus Antibody Test Kit). The results showed that 77 (43.3%) out of 178 ovine sera were seropositive for C. abortus infection, as indicated by the manufacturer's interpretation of the results. The flock was considered to be positive if at least one animal was seropositive. The flock prevalence of C. abortus in the examined flocks was 84.2% (16/19 flocks). The results of the present study indicate that C. abortus infection occurs frequently in sheep in Bosnia and Herzegovina. Key words: Bosnia and Herzegovina, Chlamydia abortus, seroprevalence, sheep U radu je opisano epidemiolosko istrazivanje s ciljem utvrdivanja seroprevalencije protutijela za vrstu Chlamydia abortus ovaca u Bosni i Hercegovini (BiH). Ovo je prvo sustavno istrazivanje ove vrste provedeno u BiH. Uzorci seruma bili su uzeti tijekom 2012. godine, a bilo je obuhvaceno 19 stada ovaca s registriranim reprodukcijskim poremecajima (pobacaji, mrtvorodenja, neplodnost) diljem drzave. Reprezentativan uzorak iz svakog stada osigurali smo jednostavnim slucajnim odabirom kojim je omoguceno otkrivanje seropozitivnosti unutar stada na 95%-tnoj razini povjerljivosti, s ocekivanom prevalencijom od 20%, sluzeci se preporukama za odredivanje potrebne velicine uzorka za otkrivanje prisutnosti bolesti. Pretrazeno je ukupno 178 uzoraka krvnog seruma ovaca na prisutnost specificnih protutijela protiv C. abortus pomocu imunoenzimnog testa (CHEKIT* Chlamydophila abortus Antibody Test Kit). Sukladno preporukama proizvodaca za tumacenje rezultata, od ukupno 178 pretrazenih uzoraka 77 (43,3%) je bilo pozitivno na C. abortus. Stado se smatralo pozitivnim ako je sadrzavalo makar jednu seropozitivnu ivotinju. Prevalencija obuhvacenih stada na C. abortus iznosila je 84,2% (16/19 stada). Rezultati ovog istrazivanja ukazuju na cinjenicu da je infekcija vrstom C. abortus kod ovaca u Bosni i Hercegovini vrlo prosirena. Kljucne rijeci: Bosna i Hercegovina, Chlamydia abortus, seroprevalencija, ovce
The aim of this research was to determine the presence of Chlamydophila abortus (C. abortus) infection in one flock of goats with a previously recorded history of reproductive failures (abortion, ...stillbirths, weak born kids) and long-term poor reproductive performances. The affected flock was from the southern region of Bosnia and Herzegovina (B&H) and consisted of 48 goats kept in semi-intensive conditions. Blood samples and vaginal swabs were collected twice during 2012 and 2013 and the sample size was estimated at a 95% confidence level, with predicted prevalence of 20%, using the recommendations for determining the required sample size necessary to detect the presence of disease in a flock. A representative sample from this flock was taken by simple random sampling. In the total of 12 blood sera that were tested for the specific antibodies against C. abortus, with the use of enzyme-linked immunosorbent assay (CHEKITR Chlamydophila abortus Antibody Test Kit), the results showed that 11 (91.7%) sera were positive for C. abortus antibodies. Vaginal swabs from all animals were analysed by a modified Chlamydiaceae-specific rtPCR test, targeting the 23S rRNA gene, to determine the presence of known Chlamydiaceae, and three (25%) samples were positive. These positive samples were subsequently tested with a test targeting the ompA gene region (ompA-rtPCR) specific for Chlamydophila abortus. All three samples were also positive using this test. Key words: Chlamydophila abortus, goats, flock, Bosnia and Herzegovina Cilj istrazivanja bio je utvrdivanje infekcije vrstom Chlamydophila abortus (C. abortus) u stadu koza s reproduktivnim poremecajima (pobacaji, mrtvorodenja, radanje slabih mladunaca i dugotrajnim losim reproduktivnim rezultatima). Pretrazeno je stado iz juzne regije Bosne i Hercegovine (BiH), a cini ga 48 koza drzanih u poluintenzivnim uvjetima. Uzorke krvi i vaginalne obriske prikupljali smo u dva navrata tijekom 2012. i 2013. godine, a potreban broj smo odredili koristeci se preporukama za odredivanje potrebne velicine uzorka za otkrivanje bolesti u stadu na 95% razini povjerljivosti i ocekivanom prevalencijom od 20%. Reprezentativan uzorak osigurali smo jednostavnim slucajnim uzorkovanjem. Od ukupno 12 pretrazenih uzoraka krvnog seruma na prisutnost specificnih protutijela protiv C. abortus pomocu imunoenzimnog testa (CHEKITR Chlamydophila abortus Antibody Test Kit), 11 (91,7%) je bilo pozitivno. Vaginalne obriske svih zivotinja potom smo analizirali modifi ciranim Chlamydiaceae-specifi cnim rtPCR za dokaz 23S rRNA gena. Tri su (25%) bila pozitivna. Potom smo pozitivne uzorke pretrazili testom za dokaz gena ompA (ompA-rtPCR) specifi cnoga za vrstu Chlamydophila abortus. Sva tri uzorka su takoder bila pozitivna. Kljucne rijeci: Chlamydophila abortus, koze, stado, Bosna i Hercegovina
The aim of this research was to investigate the use of an indirect enzyme-linked immunosorbent assay (ELISA) and Western blotting test with native excretory/secretory Fascioloides magna antigen ...(ES-Ag) in free-ranging red deer (Cervus elaphus). The research was performed on 48 red deer shot during routine culls from two different areas, considering the occurrence of fascioloidosis. After coprological examination and gross pathology examination of the livers for F. magna, serum samples were divided into three groups as infected (n = 32), uninfected (n = 13) and previously infected deer (n = 3). Indirect ELISA results were significantly higher for serum samples from infected deer (percentage of positivity (PP) 65.1 ± 18.4) than uninfected (PP 11.6 ± 13.7) and previously infected deer (PP 20.3 + or - 6.4). Samples from uninfected deer had fewer bands (30-33 and 104 kDa) on Western blotting than samples from infected deer (6, 17, 22, 27, 30-33, 40, 45 and 104 kDa). The number of alive flukes positively correlated with the ELISA results and the number of F. magna eggs per gram of faeces (EPG). The results indicated that ELISA and Western blotting test could be useful in the early diagnosis of fascioloidosis in red deer and also in control in the population of free-ranging red deer. Key words: Fascioloides magna, red deer, excretory/secretory antigens, indirect ELISA, Western blotting Cilj ovog rada bio je istraziti imunodijagnosticki potencijal ekskretomo/sekretomog antigena (ES-Ag) dobivenog od odraslih Fascioloides magna u populaciji slobodno zivucih jelena obicnih primjenom neizravnoga imunoenzimnog i Western blotting testa. Istrazivanje je provedeno na 48 jelena obicnih odstrijeljenih u okviru planiranog odstrjela na dva razlicita podrucja s obzirom na pojavu fascioloidoze. Nakon koproloske pretrage i razudbe jetara na prisutnost F. magna, uzorci seruma bili su podijeljeni u tri skupine: invadirani (n = 32), neinvadirani (n = 13) i prethodno invadirani jeleni (n = 3). Rezultati neizravnog ELISA testa bili su znacajno visi u uzorcima seruma od invadiranih jelena (postotak pozitivnosti (PP) 65,08 ± 18,40) nego neinvadiranih (PP 11,6 ± 13,7) i prethodno invadiranih jelena (PP 20,31 ± 6,43). Uzorci od invadiranih imali su nekoliko linija (30-33 i 104 kDa) primjenom Western blottinga za razliku od uzoraka od invadiranih jelena (6, 17, 22, 27, 3033, 40, 45 i 104 kDa). Broj zivih metilja u pozitivnoj je korelaciji s rezultatima ELISA testa i broja F. magna jajasca po gramu izmeta (EPG). Rezultati ukazuju da neizravna ELISA i Western blotting test mogu biti korisni s ciljem ranog otkrivanja fascioloidoze u jelena obicnog te isto tako u njenoj kontroli u populaciji slobodno zivucih jelena obicnih. Kljucne rijeci: Fascioloides magna, jelen obicni, ekskretorno/sekretorni antigeni, neizravna ELISA, Western blotting
A case of eccrine carcinoma of the interdigital foot glands in a 39-yr-old female Asian elephant (Elephas maximus) from Zagreb Zoo is described. The tumor between the toenails of the right forefoot ...was surgically removed 3 yr before postmortem examination (2003), and the histopathologic diagnosis was compound eccrine carcinoma characterized with glandular tubular and papillary proliferations, mild cellular pleomorphism, proliferation of the myoepithelial cells with mucoid secretions, and necrosis. Immunohistochemistry revealed strong immunoreactivity to S-100 protein, estrogen, and high–molecular weight cytokeratin. This elephant also had chronic renal fibrosis with uremia.
The study was undertaken to examine the effects of castration on plasma lipids and on the fatty acid profile of abdominal fat in male pheasants. Thirty pheasants reared in a commercial pheasantry ...were included in the experiment. Half the pheasants were castrated at 8 weeks of age and the other half underwent sham surgery at the same age. Plasma levels of triglycerides and high density lipoproteins (HDL) were significantly higher in the castrated pheasants. Values for cholesterol tended to be higher in castrated pheasants although without statistical significance. The fatty acid content of the abdominal fat from castrated and intact pheasants were primarily composed of oleic acid (42.58%-40.33%), followed by palmitic acid (25.25%-27.33%), linoleic (14.05%-12.65%) and stearic acid (8.95%-9.40%). Castration also significantly influenced the fatty acid composition of abdominal fat. Compared to the intact pheasants, the fatty acid content of abdominal fat from castrated pheasants contained higher values for saturated fatty acids (SFA) and lower values for unsaturated fatty acids (UFA), unsaturated to saturated fatty acids ratio (UFA/SFA) and polyunsaturated to saturated fatty acids ratio (PUFA/SFA).
PDF
