Diagnosis of meningitis Eguiguren, L; Chernow, B; Todres, I D
Pediatrics (Evanston)
81, Številka:
4
Journal Article
Recenzirano
To the Editor.—
In reviewing the medical literature about meningitis in infants, we found the review article by the Academy's Task Force on Diagnosis and Management of Meningitis1 to be helpful. ...However, we noted two important errors in Tabe 2: (1) The table (which is adapted from another article2) should be properly entitled, "Cerebrospinal Fluid Findings in Infants Without Meningitis" or "CSF Findings in Normal Infants." (2) The fifth age category in the table should be >12 months instead of 7-12 months.
Organic osmolyte and halide permeability pathways activated in epithelial HeLa cells by cell swelling were studied by radiotracer efflux techniques and single-cell volume measurements. The ...replacement of extracellular Cl
by anions that are more permeant through the volume-activated Cl
channel, as indicated by electrophysiological measurements, significantly decreased taurine efflux. In the presence of less-permeant anions, an increase in taurine efflux was observed. Simultaneous measurement of the
I, used as a tracer for Cl
, and
Htaurine efflux showed that the time courses for the two effluxes differed. In Cl
-rich medium the increase in I
efflux was transient, whereas that for taurine was sustained. Osmosensitive Cl
conductance, assessed by measuring changes in cell volume, increased rapidly after hypotonic shock. The influx of taurine was able to counteract Cl
conductance-dependent cell shrinkage but only ∼4 min after triggering cell swelling. This taurine-induced effect was blocked by DIDS. Differences in anion sensitivity, the time course of activation, and sensitivity to DIDS suggest that the main cell swelling-activated permeability pathways for taurine and Cl
are separate.
