Blood is a fluid connective tissue of human body, where it plays vital functions for the nutrition, defense and well-being of the organism. When circulating in peripheral districts, it is exposed to ...some physical stresses coming from outside the human body, as electromagnetic fields (EMFs) which can cross the skin. Such fields may interact with biomolecules possibly inducing non thermal-mediated biological effects at the cellular level. In this study, the occurrence of biochemical/biological modifications in human peripheral blood lympho-monocytes exposed in a reverberation chamber for times ranging from 1 to 20 h to EMFs at 1.8 GHz frequency and 200 V/m electric field strength was investigated. Morphological analysis of adherent cells unveiled, in some of these, appearance of an enlarged and deformed shape after EMFs exposure. Raman spectra of the nuclear compartment of cells exposed to EMFs revealed the onset of biochemical modifications, mainly consisting in the reduction of the DNA backbone-linked vibrational modes. Respirometric measurements of mitochondrial activity in intact lympho-monocytes resulted in increase of the resting oxygen consumption rate after 20 h of exposure, which was coupled to a significant increase of the FoF1-ATP synthase-related oxygen consumption. Notably, at lower time-intervals of EMFs exposure (i.e. 5 and 12 h) a large increase of the proton leak-related respiration was observed which, however, recovered at control levels after 20 h exposure. Confocal microscopy analysis of the mitochondrial membrane potential supported the respiratory activities whereas no significant variations in the mitochondrial mass/morphology was observed in EMFs-exposed lympho-monocytes. Finally, altered redox homeostasis was shown in EMFs-exposed lympho-monocytes, which progressed differently in nucleated cellular subsets. This results suggest the occurrence of adaptive mechanisms put in action, likely via redox signaling, to compensate for early impairments of the oxidative phosphorylation system caused by exposure to EMFs. Overall the data presented warn for health safety of people involved in long-term exposure to electromagnetic fields, although further studies are required to pinpoint the leukocyte cellular subset(s) selectively targeted by the EMFs action and the mechanisms by which it is achieved.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The VLF/LF radio signals method for studying preseimic activity is applied to the Abruzzo earthquake (M=6.3, 6 April 2009). The data collected by three receivers located in Moscow (Russia), Graz ...(Austria) and Bari (Italy) at about 3000 km, 1000 km and 500 km from the epicenter were used. The signals received from the Sardinia (20.27 kHz) and the Sicily (45.9 kHz) transmitters, both located in Italy, were compared with those received from the Iceland (37.5 kHz), the Great Britain (19.58 kHz) and the Germany (23.4 kHz) transmitters. The propagation paths of the two Italian transmitters cross the epicentral area (seismic paths) unlike the paths of the other three signals (control paths). Using two different analyses, that are the study of the night-time signal and the research of shifts in the evening terminator times, clear anomalies were revealed 2–8 days before the occurrence of the Abruzzo earthquake in the seismic paths, while no anomalies have been found in the control paths.
In the last years disturbances in VLF/LF radio signals related to seismic activity have been presented. The radio data were collected by receivers located on the ground or on satellites. The ...ground-based research implies systematic data collection by a network of receivers. Since 2000 the "Pacific VLF network", conducted by Japanese researchers, has been in operation. During 2008 a radio receiver was developed by the Italian factory Elettronika (Palo del Colle, Bari). The receiver is equipment working in VLF and LF bands. It can monitor 10 frequencies distributed in these bands and, for each of them, it saves the power level. At the beginning of 2009, five receivers were made for the realization of the "European VLF/LF Network"; two were planned for Italy and one for Greece, Turkey and Romania, respectively. In 2010 the network was enlarged to include a new receiver installed in Portugal. In this work, first the receiver and its setting up in the different places are described. Then, several disturbances in the radio signals related to the transmitters, receivers, meteorological/geomagnetic conditions are presented and described.
Background Monoclonal gammopathies are haematological conditions characterized by the clonal proliferation of plasma cells which produce a monoclonal immunoglobulin that accumulates in the blood. ...They have already been reported during treatment with a range of drugs but never before during treatment with the anti‐TNF‐α treatments: adalimumab, etanercept and infliximab currently used in the therapy of moderate‐severe psoriasis and psoriatic arthritis.
Objective This is a case series describing the development of MGUS in psoriatic patients treated with anti‐TNF‐α.
Methods Three hundred patients receiving an anti‐TNF‐α treatment for chronic plaque psoriasis or psoriatic arthritis in a clinical setting in Italy, These patients were screened through serum protein electrophoresis to investigate the possible development of MGUS.
Results Eight patients were found to have developed monoclonal gammopathy of undetermined significance. The median treatment duration for the eight patients was 1 year with excessive IgG present in five patients, IgM accumulation in one patient and a double monoclonal component in two patients.
Conclusion Our data suggest that there may be an association between anti‐TNF‐α therapy and development of MGUS.
On 6 April 2009 a strong (Mw=6.3) earthquake occurred in the Abruzzo region (central Italy). Since 1996, the intensity of CLT (f=189 kHz, Sicily, Italy), MCO (f=216 kHz, France) and CZE (f=270 kHz, ...Czech Republic) broadcast signals has been collected with a ten minutes sampling rate by a receiver operating in a place located about 13 km far from the epicenter. During March 2009, the old receiver was substituted with a new one able to measure, with one minute sampling rate, the intensity of five VLF signals and five LF signals radiated by transmitters located in different zones of Europe. The MCO and CZE transmitters mentioned above are included among them. From 31 March to 1 April the intensity of the MCO radio signal dropped and this drop was observed only in this signal. The possibility that the drop was connected to problems in the transmitter or in the receiver was investigated and excluded. So, the drop indicates a defocusing of the radiated signal. Since no particular meteorological situation along the path transmitter-receiver happened, the defocusing must be related to other causes, and a possibility is presented that it is a precursor of the Abruzzo earthquake.
We report the information that in the days of the radio anomaly presented in the paper Biagi et al. (2009) an interruption of the broadcasting from the transmitter (RMC, France) happened. It remains ...unclear if the action resulted in a complete power off of the system, or in a reduction in the radiated power, and if this has affected France only, or every direction. Should a complete power off have occurred, the proposed pre-seismic defocusing is inexistent. Our doubts on this action are reported.
In February 2002, a receiver was put into operation at the Department of Physics of Bari University (Southern Italy) to record VLF-LF radio signals. The intensity and the phase of the signals ...transmitted by GB (f=16 kHz, United Kingdom), FR (f=20.9 kHz, France), GE (f=23.4 kHz, Germany), IC (f=37.5 kHz, Island) and IT (f=54 kHz, Sicily, Italy) has been monitored with a 5 s sampling rate. The intensity raw data averaged over 10 min, from February 2002 to April 2006, have been analysed. Several decreases of the electric field intensity of the radio signals with a duration of some days were revealed, generally occurring in not concomitant periods. The GE signal decreases systematically in winter and summer each year and so, it could be supposed that such decreases are related to the transmitter. On the contrary, all the other decreases pointed out are sporadic and the previous justification does not seem realistic. On the time occurrence of these decreases, the geomagnetic activity, the meteorological conditions in the receiver area and the regional seismic activity were investigated. The main result is that, generally, a pre or post seismic effect seems to give the most convincing justification. The effect seems to appear for earthquakes with magnitude M≥4.3, when the epicentres are within the third Fresnel zone of the radio signals or near enough to some radio path.
On 22 August 2005 an earthquake with magnitude M=4.7 occurred in the Anzio (central Italy) offshore area. From 2002, a VLF-LF radio receiver is into operation in Bari (southern Italy). The intensity ...and the phase of the signals transmitted by GB (f=16 kHz, United Kingdom), FR (f=20.9 kHz, France), GE (f=23.4 kHz, Germany), IC (f=37.5 kHz, Island) and IT (f=54 kHz, Sicily, Italy) has been monitored with a 5 s sampling rate. The previous epicenter is near enough to some of the radio paths and the data collected were studied in order to reveal possible seismic effects. The raw analysis revealed a clear drop in the intensity of the FR radio signal on 19 August. Then the wavelet analysis was applied to the intensity and the phase data of the different radio signals. In the mentioned day an increase in the band 60–120 min was revealed in the spectra of the FR signal. Then the principal component analysis was applied and again the 19 August stood up as an anomalous day for the FR radio signal. The path of this signal, among the paths of the five radio signals collected by the Bari receiver, is the nearest to the mentioned epicentre and the anomaly revealed on 19 August appears as a precursor of the earthquake. This result confirms the possible precursor revealed by other researchers in the air Rn content in a site located 5 miles far from the epicenter.
A system generating 1.8 GHz electromagnetic fields for bio-medical and behavioral study on laboratory animals was designed and implemented. The system is based on a reverberation chamber. An input ...power up to 5 W can be sent to an indoor transmitting antenna and an electric field strength (E) more than 90 V/m can be reached inside the chamber. The system was characterized at different input powers measuring E in different points by means of a miniature sensor. Then, boxes with 300 cc of physiological liquid inside were realized as simple phantoms simulating laboratory animals (rats) and E inside the liquid was measured, performing several simulations by moving the phantoms (1,2) in the chamber and/or putting them still in different positions. On the basis of these measurements, the SAR (Specific Absorption Rate) and the Pe (power efficiency = SAR/input power) were determined at different powers. The actual system is characterized by a low power efficiency with respect to the "in vivo" exposition systems based on transversal electromagnetic (TEM) cells. Its advantage is to have inside the chamber a habitat similar to the usual one for the laboratory animals.
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