Electrochemical behavior of cerium hexacyanoferrate (CeHCF) incorporated on multi-walled carbon nanotubes (MWNTs) modified GC electrode is investigated by scanning electron microscopy (SEM) and ...electrochemical techniques. The CeHCF/MWNT/GC electrode showed potent electrocatalytic activity toward the electrochemical oxidation of tryptophan in phosphate buffer solution (pH 7.0) with a diminution of the overpotential of 240
mV. The anodic peak currents increased linearly with the concentration of tryptophan in the range of 2.0
×
10
−7 to 1.0
×
10
−4
M with a detection limit of 2.0
×
10
−8
M (at a S/N
=
3). And the determination of tryptophan in pharmaceutical samples was satisfactory.
Objective: Investigating the protective effect of MVE was the purpose of this study, which performed on LPS-induced liver injury in aged mice. Methods: The mice were randomized into the groups of ...normal control, MVE, LPS and LPS+MVE. The pathological changes of liver tissue and the expression of myeloperoxidase were observed by hematoxylin-eosin staining and immunohistochemical staining. The levels of IL-1β, IL-17, CXCL1, CXCL17 and other factors in liver were measured. Western blotting was used to detect the expressions of TLR4 and NF-κB p65 in liver tissue samples. Results: The MVE could effectively decline the infiltration of neutrophils and inflammatory cells, significantly decrease the levels of ALT, AST, ROS (P<0.01), pro-inflammatory cytokines and neutrophil chemokines (P<0.05) in the liver of LPS model mice, and decrease protein expressions of TLR4 and NF-κB p65 in inflammatory pathway (P<0.001). Conclusion: The MVE could improve the liver injury of mice with acute inflammation induced by LPS, and exe
Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease with the colorectum as its major target organ. Involvement of the upper gastrointestinal tract in UC is rare and presents ...with nonspecific endoscopic and microscopic characteristics. Recent studies have demonstrated proteinase 3 antineutrophil cytoplasmic antibody (PR3-ANCA) to be a serological marker for differentiating UC from Crohn's disease in children and for detecting disease activity and nonresponse to steroid therapy and antitumor necrotizing factor-α agents. Herein, we report a 13-year-old female patient mainly presenting with recurrent bilious vomiting who was initially diagnosed with acute gastroenteritis. Intestinal pseudo-obstruction was confirmed through observation of a patent but segmentally dilated jejunum in the barium follow-through examination and other imaging; such obstruction can be attributed to backwash ileitis, superior mesenteric artery syndrome, ileus due to hypokalemia, or PR3-associated enteritis. Laboratory data revealed leukocytosis with neutrophil predominance and serum antinuclear antibody and PR3-ANCA positivity. Overlapping syndrome with autoimmune diseases was suspected. Pathology revealed a crypt abscess with aggregates of neutrophils consistent with UC but did not indicate vasculitis. The
immunohistochemical staining revealed PR3 density mainly in the colon and focally in the duodenum. To our knowledge, this is the first case report with
pathological evidence of PR3 in inflamed intestinal tissues in a patient with UC and with rare initial presentation of intestinal pseudo-obstruction-induced recurrent bilious vomiting. Whether the clinical features of the present case constitute overlap syndrome with other autoimmune disease or a disease variation of UC warrants further investigation. Notably, the patient's serum PR3-ANCA titers remained high in coincidence with increased disease activity and nonresponse to steroid therapy, but became lower after infliximab treatment. PR3-ANCA as a potential serum biomarker to aid in making differential diagnoses of UC in children, correlating disease activity, and predicting therapeutic responses was also reviewed.
The polymorphism +49A/G in the cytotoxic T lymphocyte-associated antigen 4 (
CTLA
-
4
) gene has been implicated in susceptibility to Hashimoto’s thyroiditis (HT), but the findings are not clear-cut. ...This study aimed to investigate the association between
CTLA
-
4
polymorphisms and HT risk using an updated meta-analysis. A meta-analysis was carried out of 14 previous studies that investigated the
CTLA
-
4
+49A/G polymorphism and HT risk. +49A/G was associated with a significantly increased HT risk in both allele analysis and all genetic models (allele analysis: G vs. A:
P
< 0.001,
OR
= 1.379, 95 % CI = 1.244–1.529). Subgroup analysis by ethnicity showed a significantly increased HT risk with the G allele and all other genetic models in the Asian subgroup (
P
< 0.001). In the Caucasian subgroup, no significant association was detected between the
CTLA
-
4
+49 G allele and HT, or in the genetic model analysis (
P
= 0.05). This gene-based analysis indicates that the cumulative effect of the +49A/G polymorphism in
CTLA
-
4
is associated with HT in Asians, but appears to have no effect on HT in Caucasians.
•Acrylamide (ACR) induces neurotoxicity by forming irreversible adducts with thiolates.•The ACR-adducted proteome was characterized in mesencephalic dopaminergic cells (N27).•Less than 1.0% (103 ...peptides) of the N27 cell proteome contained ACR adducts.•ACR-adducted proteins are associated with neurotoxicity and neurodegeneration.•The catalog of adducted proteins offers new insight into neurotoxic mechanisms of ACR.
Acrylamide (ACR) is an electrophilic unsaturated carbonyl derivative that produces neurotoxicity by forming irreversible Michael-type adducts with nucleophilic sulfhydryl thiolate groups on cysteine residues of neuronal proteins. Identifying specific proteins targeted by ACR can lead to a better mechanistic understanding of the corresponding neurotoxicity. Therefore, in the present study, the ACR-adducted proteome in exposed primary immortalized mesencephalic dopaminergic cells (N27) was determined using tandem mass spectrometry (LTQ-Orbitrap). N27 cells were characterized based on the presumed involvement of CNS dopaminergic damage in ACR neurotoxicity. Shotgun proteomics identified a total of 15,243 peptides in N27 cells of which 103 unique peptides exhibited ACR-adducted Cys groups. These peptides were derived from 100 individual proteins and therefore ~0.7% of the N27 cell proteome was adducted. Proteins that contained ACR adducts on multiple peptides included annexin A1 and pleckstrin homology domain-containing family M member 1. Sub-network enrichment analyses indicated that ACR-adducted proteins were involved in processes associated with neuron toxicity, diabetes, inflammation, nerve degeneration and atherosclerosis. These results provide detailed information regarding the ACR-adducted proteome in a dopaminergic cell line. The catalog of affected proteins indicates the molecular sites of ACR action and the respective roles of these proteins in cellular processes can offer insight into the corresponding neurotoxic mechanism.
In order to further enrich the existing manufacturing methods and approaches of magnesium alloy sheet forming, a new process of circumferential extending extrusion forming of magnesium alloy sheet, ...which focuses on the process principle and characteristics of the method, is proposed in this paper. Through theoretical analysis, technological theoretical models such as the dimension relationship between the original billet and the formed sheet and the extrusion speed were deduced. The circumferential extending extrusion process of AZ31 magnesium alloy sheets was experimented. The results show that the backpressure ring of pure aluminum can change the stress state of the sheet during extending extrusion and effectively restrain the change of sheet thinning trend and cracking defects at the outer edge caused by tensile stress, and the product quality is improved remarkably. According to the actual requirements, the size of the required sheets can be customized. It can be seen from the comparison that as the distance from the axis increases, the average grain size of the product decreases remarkably, and and the degree of recrystallization is higher. Meanwhile, the microstructure is refined. With the maturity of the process, it is expected to provide a new idea for the short-process forming of high-performance lightweight alloy sheets.
The development of high-efficiency and robust photocatalysts is imperative and challenging in advanced oxidation processes (AOPs). Herein, magnetically separable ZnFe2O4/MoS2 photocatalyst was ...prepared by a simple hydrothermal method. The degradation characteristics of several organic pollutants (rhodamine B (RhB), methyl orange, methylene blue, and antibiotic tetracycline) were investigated with/without the addition of H2O2 under visible light irradiation. It is found that the synergistic effect of Z-scheme ZnFe2O4/MoS2 photocatalyst and photo-fenton-like reaction promotes the decomposition of H2O2, accelerates the separation efficiency of photogenerated electrons and holes, and thus improves the photocatalytic activity. The RhB photo-degradation of the constructed Z-scheme ZnFe2O4/MoS2 (96.3%) photocatalyst in the absence of H2O2 for 100 min is better than that of MoS2 (74.8%) and ZnFe2O4 (6.2%), with a rate constant of 0.0332 min−1. The slight amount of H2O2 boosts the photocatalytic performance significantly. Its degradation rate is 98.6% within 8 min with the rate constant of 0.396 min−1, 12 times higher than that of ZnFe2O4/MoS2 photocatalyst without H2O2. The magnetic separation, recycling stability and neutral solution process are also confirmed in ZnFe2O4/MoS2 system, indicating the enormous potential of this photocatalyst in environmental remediation and waste water treatment. The hole-mediated oxidation mechanism has been proposed instead of conventional active radicals of •OH based on the free radicals capture and electron spin resonance experiments. MoS2 plays a crucial role in facilitating the H2O2 decomposition and realizing the conversion circulation from Fe3+ to Fe2+ by the redox cycling as a co-catalyst. This study provides insights into the photocatalytic mechanism of Z-scheme photo-Fenton heterojunction photocatalysts, conducive to the development of high-efficient and stable photocatalysts in AOPs.
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•The magnetically separable ZnFe2O4/MoS2 photocatalyst are prepared by a hydrothermal method.•The synergistic effect of Z-scheme heterojunction and photo-like Fenton reaction promotes the separation efficiency of photogenerated electrons and holes.•ZnFe2O4/MoS2 system is a hole-mediated oxidation mechanism.•The addition of a small amount of H2O2 showed excellent degradation performance.
•A new fluorogenic probe (TPAN-Asn) for l-asparaginase detection was developed.•TPAN-Asn exhibited high selectivity and activity in complex physiological environments.•TPAN-Asn was successfully ...applied to the detection of l-asparaginase in serum samples.•TPAN-Asn is more robust and convenient to use than traditional Nessler reaction.•TPAN-Asn has great potential in the analysis of l-asparaginase-based cancer therapy.
Fluorescence-based in vitro assays are highly sensitive, selective and convenient to use, which is suitable for qualitative and quantitative detection of various types of biological samples. Herein, we designed and synthesized a novel fluorogenic probe TPAN-Asn for in vitro l-asparaginase detection. TPAN-Asn exhibited selective and robust response to l-asparaginase over various anions, cations and amino acids. More importantly, we have demonstrated that TPAN-Asn is able to accurately quantify the amount of l-asparaginase in patient serum samples. These results suggest that TPAN-Asn holds great potential in the benchmarking and analysis of l-asparaginase-based cancer therapy.
Abstract Objectives —To explore the association of baseline characteristics and the outcome of acute basilar artery occlusion (BAO) patients following stent retriever based thrombectomy (SRT). ...Methods —Clinical and imaging information of consecutive SRT treated BAO patients from a comprehensive stroke center and up-to-date literatures were reviewed respectively. The impact of baseline variables towards favorable outcome was evaluated using subgroup analysis and odds ratio extracted from published data resource together with single center records using pooled analysis. Results —Nineteen cases from our center and 15 published studies involving 487 cases were included. Estimated pooled favorable outcome rate was 0.3746 95% CI 0.3165, 0.4327, mortality rate was 0.2950 95% CI 0.2390; 0.3510. And pooled estimates showed that successful reperfusion (modified thrombolysis in cerebral ischemia scale 2b or 3) gained by SRT alone was 0.7317 95% CI 0.6532; 0.8102, final successful reperfusion rate with or without additional reperfusion procedures was 0.8834 95% CI 0.8279; 0.9390. Univariate analysis indicated that patients with successful reperfusion (OR=2.05, P=0.05), distal segment occlusion (OR=2.03, P=0.03), cardio-embolus origin (OR=2.13, P=0.01) were more likely to have favorable outcome(modified Rankin Scale ≤2 at 3 months). Study series that applied intra-arterial thrombolysis, angioplasty or stenting as rescuing therapy had higher successful reperfusion rate, but they didn’t show higher rate of favorable outcome. Conclusions —SRT with or without additional treatment appeared to be effective for the treatment of BAO. Successful reperfusion, distal segment occlusion, cardiac embolism were associated with favorable outcome. The overall benefit of lesions requiring additional reperfusion therapy was unclear.
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