NEB414 is the original source strain for the restriction enzyme BclI. Its complete sequence and full methylome were determined using single-molecule real-time sequencing.
65 is the original source strain for the restriction enzyme Acc65I. Its complete sequence and full methylome were determined using single-molecule real-time (SMRT) sequencing.
P63, the major regulator of epithelial development/differentiation, is mutated in humanectodermal dysplasias, such as ankyloblepharon, ectodermal dysplasia and clefting (AEC). Werecently identified ...that p63? physically associated with mRNA processing/splicing proteins. Wepreviously showed that p63 mutations mapped to the sterile ?-motif led to disruption of theseinteractions and modulated an aberrant splicing of keratinocyte growth factor receptorcontributing into molecular mechanism underlying AEC phenotype.
To further investigate the molecular mechanisms associated with AEC syndrome weestablished the cellular model for this disorder by stable introduction of mutated allele L514Fof p63? into immortalized keratinocyte cells. We showed that mutated ?Np63? mediated anaberrant splicing of its own p63 mRNA transcript, which in turn led to accumulation ofproteasome-resistant C- terminal truncated p63. The truncated p63 failed to associate with theC-terminal domain of RNA polymerase II through SRA4 protein and, therefore affectedkeratinocyte proliferation, differentiation and survival and may strongly contribute to AECphenotype.
The genome of halophilic archaeon Haloarcula sinaiiensis ATCC 33800 was sequenced and assembled and comprises seven replicons. Four m6A and one m4C modified motifs and their responsible ...methyltransferase genes have been identified in the genome by single-molecule real-time (SMRT) sequencing and bioinformatic analysis.
This paper describes the complete genome sequences and methylome analysis of six psychrotrophic strains isolated from perennially ice-covered Lake Untersee in Antarctica.
The P53 homolog
p63 encodes multiple proteins with transactivating, apoptosis-inducing, and oncogenic activities. We showed that
p63 is amplified and that ΔNp63 isotypes are overexpressed in squamous ...cell carcinoma (SCC) and enhance oncogenic growth in vitro and in vivo. Moreover, p53 associated with ΔNp63α and mediated its degradation. Here, we report that ΔNp63 associates with the B56α regulatory subunit of protein phosphatase 2A (PP2A) and glycogen synthase kinase 3β (GSK3β), leading to a dramatic inhibition of PP2A-mediated GSK3β reactivation. The inhibitory effect of ΔNp63 on GSK3β mediates a decrease in phosphorylation levels of β-catenin, which induces intranuclear accumulation of β-catenin and activates β-catenin-dependent transcription. Our results suggest that ΔNp63 isotypes act as positive regulators of the β-catenin signaling pathway, providing a basis for their oncogenic properties.
Here, we report the complete closed genome sequence and methylome analysis of
strain D-401 (DSM 14945, UNIQEMU 779), which is quite different from the previously described
neotype strain D-402
(DSM ...14946, UNIQEM U 779) with regard to morphology and lithotrophic growth in the presence of thiosulfate.
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