Purpose of Review
This review a highlights that to use artificial intelligence (AI) tools effectively for hypertension research, a new foundation to further understand the biology of hypertension ...needs to occur by leveraging genome and RNA sequencing technology and derived tools on a broad scale in hypertension.
Recent Findings
For the last few years, progress in research and management of essential hypertension has been stagnating while at the same time, the sequencing of the human genome has been generating many new research tools and opportunities to investigate the biology of hypertension. Cancer research has applied modern tools derived from DNA and RNA sequencing on a large scale, enabling the improved understanding of cancer biology and leading to many clinical applications. Compared with cancer, studies in hypertension, using whole genome, exome, or RNA sequencing tools, total less than 2% of the number cancer studies. While true, sequencing the genome of cancer tissue has provided cancer research an advantage, DNA and RNA sequencing derived tools can also be used in hypertension to generate new understanding how complex protein network, in non-cancer tissue, adapts and learns to be effective when for example, somatic mutations or environmental inputs change the gene expression profiles at different network nodes. The amount of data and differences in clinical condition classification at the individual sample level might be of such magnitude to overwhelm and stretch comprehension. Here is the opportunity to use AI tools for the analysis of data streams derived from DNA and RNA sequencing tools combined with clinical data to generate new hypotheses leading to the discovery of mechanisms and potential target molecules from which drugs or treatments can be developed and tested.
Summary
Basic and clinical research taking advantage of new gene sequencing-based tools, to uncover mechanisms how complex protein networks regulate blood pressure in health and disease, will be critical to lift hypertension research and management from its stagnation. The use of AI analytic tools will help leverage such insights. However, applying AI tools to vast amounts of data that certainly exist in hypertension, without taking advantage of new gene sequencing-based research tools, will generate questionable results and will miss many new potential molecular targets and possibly treatments. Without such approaches, the vision of precision medicine for hypertension will be hard to accomplish and most likely not occur in the near future.
Light is an important component in poultry production, and it may impact bird behavior, an important component of animal welfare. Light-emitting diode (LED) lamps are of interest for broiler ...production since they are inexpensive to run and provide monochromatic colors. This study aimed to understand the impact of three light colors (blue, green, or white), provided by LED lighting, on behavioral expression, stress and fear levels of broilers. A total of 14,256 male and female broilers of 2 genotypes (Ross EPMx708 and Ross YPMx708) were housed in 9 rooms in 2 blocked trials (3 room replicates per light per trial), with sexes and genotypes housed in 12 separate pens per room. Behavioral expression was recorded using an infrared camera and analyzed using a scan sampling technique. To assess fear, 3 tests were conducted: tonic immobility, novel object, and response to observer. Blood was collected to evaluate chronic stress using the heterophil:lymphocyte (H:L) ratio. Data were statistically analyzed using SAS (MIXED procedure) in a 3 × 2 × 2 factorial design, with lighting treatment nested within room. Fear tests indicated reduced fear levels in birds raised under blue light (lower latency to rise during the tonic immobility test and a lower percentage of birds moving due to the passage by of an observer). No differences were observed for the novel object test. Light color resulted in changes in stress levels, indicated by a lower H:L ratio for broilers raised under blue light compared to those raised under white light. Behavior was influenced by light color, especially at 33 to 34 d of age, where birds raised under white light were more active, and birds raised under blue light spent more time resting. Overall, results indicated that light color has minor influences on behavioral expression. Utilizing blue light during the brooding and rearing phase leads to lower stress and a reduction in fear, suggesting that blue light may improve the emotional states of fear and stress, thereby improving the welfare of poultry.
•Financial literacy research focuses on why, how, and when people acquire financial knowledge, shape their financial attitudes, and adapt their financial behaviors.•The literature demonstrates that ...some demographic characteristics highly correlate with financial literacy.•Demographic factors mask the ultimate determinants of financial literacy acquisition.•We offer experimental evidence supporting the key role of cognitive ability in financial literacy acquisition.•After controlling for some of the main confounding factors identified in the literature, we find that individuals with higher cognitive abilities are more financially literate.
Financial literacy research focuses on why, how, and when people acquire financial knowledge, shape their financial attitudes, and adapt their financial behaviors. The literature demonstrates that some demographic characteristics highly correlate with financial literacy. However, demographic factors often mask the ultimate determinants of financial literacy acquisition such as risk aversion, time preferences, cognitive and behavioral biases, personality traits, cognitive and non-cognitive abilities, among others. Theory suggests that cognitive ability is one of the fundamental factors in explaining financial literacy. We offer experimental evidence supporting the key role of cognitive ability in financial literacy acquisition. Our experimental setting allows us to (a) overcome particular limitations of the traditional multiple-choice questions survey designs, (b) provide compatible incentives to make participants exert an appropriate level of effort to solve the assigned tasks, and (c) use a well-known measure of cognitive abilities. We find that individuals with higher cognitive abilities are more financially literate. Our main result holds even after controlling for some of the main confounding factors identified in the literature. In contrast to previous studies, we find no role for gender in explaining financial literacy once we control for cognitive abilities.
AIM: Evaluate technologically relevant properties from bacteriocin‐producing strains to use as starter/adjunct cultures in cheese making. METHODS AND RESULTS: Eight isolates obtained from Pico cheese ...produced in Azores (Portugal) were found to produce bacteriocins against Listeria monocytogenes and three isolates against Clostridium perfringens. They were identified as Lactococcus lactis and Enterococcus faecalis and submitted to technological tests: growth at different conditions of temperature and salt, acid production, proteolysis, lipolysis, coexistence, enzymatic profile and autolytic capacity. Safety evaluation was performed by evaluating haemolytic, gelatinase and DNase activity, resistance to antibiotics and the presence of virulence genes. Some isolates presented good technological features such as high autolytic activity, acid and diacetyl production. Lactococcus lactis was negative for all virulence genes tested and inhibit the growth of all Lactic acid bacteria (LAB) isolates. Enterococci were positive for the presence of some virulence genes, but none of the isolates were classified as resistant to important antibiotics. CONCLUSIONS: The bacteriocin‐producing Lc. lactis present good potential for application in food as adjunct culture in cheese production. The study also reveals good technological features for some Enterococcus isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: Bacteriocin‐producing strains presented important technological properties to be exploited as new adjunct culture for the dairy industry, influencing flavour development and improve safety.
Light color during brooding and rearing may impact broiler production; however, literature results are inconsistent. To address this, the effects of 3 wavelength spectra on broiler performance in 2 ...sex and 2 genotypes (Ross YPMx708 and EPMx708) were studied. Broilers were raised (d 0–35) under wavelength programs provided by LED light bulbs (blue (455 nm), green (510 nm) or white) under similar intensities (clux). Two trials were conducted (total number of birds = 14,256; 6 room replications per lighting treatment; 18 replicate pens per light × sex × genotype). Data were analyzed as a 3 × 2 × 2 (wavelength × sex × genotype) factorial design, with trial as a random variable block and wavelength nested within rooms (Proc Mixed, SAS 9.4). Birds raised under white light were heavier than under blue or green light at d7 (P = 0.004), and green at d14 (P = 0.03). Feed intake, gain-to-feed efficiency and flock uniformity (d15, 28) did not differ. Mortality only differed at wk 5, when broilers raised under white light had higher mortality than those raised under blue (P = 0.03). YPM-708 were heavier at 21 d (P = 0.007), 28 d (P = 0.001), and 35 d (P < 0.0001), had a better total feed conversion rate (P < 0.0001), higher mortality for wk 1 (P = 0.001), lower mortality during the last wk (P = 0.02) and better uniformity at 28 d (P = 0.01) than EPM-708 broilers. Males were heavier at all measured ages except d0 (d7-P = 0.03, other weeks P < 0.0001), had better total feed conversion (P < 0.0001), increased weekly mortality except for wk 1 (wk2-P = 0.04, wk3-P = 0.002, wk4, 5, and total-P = 0.0001) and were less uniform (P = 0.0002) than females. YPM-708 and EPM-708 males had higher total feed intake (P < 0.0001), and males raised under white light had higher mortality than females raised under white or blue light (P = 0.01). To conclude, the use of specific light colors (blue and green) had only minor impacts on broiler production when light intensity was equalized and balanced for bird spectral sensitivity, and its use to improve productivity does not appear to be advantageous for broilers in a commercial setting.
Atmospheric acidity is increasingly determined by carbon dioxide and organic acids
. Among the latter, formic acid facilitates the nucleation of cloud droplets
and contributes to the acidity of ...clouds and rainwater
. At present, chemistry-climate models greatly underestimate the atmospheric burden of formic acid, because key processes related to its sources and sinks remain poorly understood
. Here we present atmospheric chamber experiments that show that formaldehyde is efficiently converted to gaseous formic acid via a multiphase pathway that involves its hydrated form, methanediol. In warm cloud droplets, methanediol undergoes fast outgassing but slow dehydration. Using a chemistry-climate model, we estimate that the gas-phase oxidation of methanediol produces up to four times more formic acid than all other known chemical sources combined. Our findings reconcile model predictions and measurements of formic acid abundance. The additional formic acid burden increases atmospheric acidity by reducing the pH of clouds and rainwater by up to 0.3. The diol mechanism presented here probably applies to other aldehydes and may help to explain the high atmospheric levels of other organic acids that affect aerosol growth and cloud evolution.
Light is a powerful management tool in poultry production systems, affecting productivity, physiology, and behavior. The objective of this study was to understand the impacts of three light colors ...(blue, green, or white) on broiler health. Broilers (N = 14,256) were raised in floor pens with fresh litter from 0 to 35 d in 9 rooms (2 blocked trials). Additionally, 2 genotypes (Ross YPMx708 and EPMx708) and sex were studied (6 room replications per lighting treatment and 18 pen replicates per sex × genotype × lighting program). Blood samples and tissue samples from the retina and the pineal gland were collected from birds (16–18 d of age) 9 times in one 24-hr period per trial, then analyzed to determine melatonin levels (pg/mL). Mobility was assessed via gait scoring, using a 0 to 5 scale at 31 to 32 d of age. Footpad dermatitis was assessed using a 0 to 4 scale, and litter quality by a subjective scoring system (scores ranging from 0–4). Mortality and morbidity causes were identified through necropsies performed by pathologists. Data were analyzed as a 3 × 2 × 2 factorial design, with trial as a random variable block and lighting treatment nested within rooms (MIXED procedure, SAS). Birds raised under blue light had lower serum melatonin levels during one time-point during the scotophase, but no other differences were noted. No effect of light color was observed for melatonin produced in the tissues, nor mobility and footpad dermatitis. An interaction was noted for litter quality where a higher percentage of pens housing YPM-708 broilers had litter categorized into dry, but not easily moved with the foot (category 1). Males had higher incidence of infectious and metabolic deaths than females. Interactions were observed between light and sex, where males raised under white light had a higher incidence of skeletal causes of mortality. Overall, the results showed that light color had minor impacts only on melatonin levels, mobility, footpad dermatitis, litter quality, and cause of mortality.
Objective
To evaluate the rate of change in serum lipids and the factors associated with their variations, stratifying for pre‐pregnancy body mass index (BMI) categories.
Design
Prospective cohort.
...Setting
Public Health centre, Rio de Janeiro, Brazil.
Population
Two hundred and twenty‐five healthy pregnant women recruited between 2009 and 2011.
Methods
Women were evaluated during the three trimesters of pregnancy (5th–13th, 20th–26th and 30th–36th gestational weeks). Pre‐pregnancy BMI (kg/m²) was classified as normal weight (NW = 18.5–24.9), overweight (OW = 25.0–29.9) or obese (OB ≥ 30.0). The independent variables included maternal socioeconomic, demographic, biochemical and lifestyle factors. We performed linear mixed‐effects models adjusted for gestational age and body weight, reporting coefficient (β) and 95% confidence interval (95% CI).
Main outcome measures
Longitudinal total cholesterol (TC), high‐density lipoprotein (HDL‐c), low‐density lipoprotein (LDL‐c) and triglyceride (TG) measurements.
Results
OW and OB women presented higher mean TG, TC and LDL‐c compared with their NW counterparts (P < 0.05). The mean HDL‐c concentrations were higher in NW than in OB women (P = 0.03). OW and OB women presented lower serum TC (βOW = −0.014; 95% CI = −0.026 to −0.002; P = 0.022; βOB = −0.015; 95% CI −0.015 to 0.001; P = 0.066) and LDL‐c (βOW = −0.012; 95% CI = −0.021 to −0.002; P = 0.017; βOB = −0.018; 95% CI = −0.031 to −0.005; P = 0.005) rates of change (mmol/l per gestational week) compared with the NW. Pre‐gestational BMI was the main factor associated with the rate of changes in TC and LDL‐c concentrations.
Conclusion
Pre‐pregnancy BMI was the main factor associated with the rate of change in TC and LDL‐c throughout pregnancy, and OW and OB women presented lower rates of change compared with NW controls.
Despite the recent growth in interest for metal additive manufacturing (AM) in the biomedical and aerospace industries, variability in the performance, composition, and microstructure of AM parts ...remains a major impediment to its widespread adoption. The underlying physical mechanisms, which cause variability, as well as the scale and nature of variability are not well understood, and current methods are ineffective at capturing these details. Here, a Nickel-Titanium alloy is used as a sensory material in order to quantitatively, and rather rapidly, observe compositional and/or microstructural variability in selective laser melting manufactured parts; thereby providing a means to evaluate the role of process parameters on the variability. We perform detailed microstructural investigations using transmission electron microscopy at various locations to reveal the origins of microstructural variability in this sensory material. This approach helped reveal how reducing the distance between adjacent laser scans below a critical value greatly reduces both the in-sample and sample-to-sample variability. Microstructural investigations revealed that when the laser scan distance is wide, there is an inhomogeneity in subgrain size, precipitate distribution, and dislocation density in the microstructure, responsible for the observed variability. These results provide an important first step towards understanding the nature of variability in additively manufactured parts.
Aims To describe, at high resolution, the bacterial population dynamics and chemical transformations during the ensiling of alfalfa and subsequent exposure to air. Methods and Results Samples of ...alfalfa, ensiled alfalfa and silage exposed to air were collected and their bacterial population structures compared using 16S rRNA gene libraries containing approximately 1900 sequences each. Cultural and chemical analyses were also performed to complement the 16S gene sequence data. Sequence analysis revealed significant differences (P < 0·05) in the bacterial populations at each time point. The alfalfa-derived library contained mostly sequences associated with the Gammaproteobacteria (including the genera: Enterobacter, Erwinia and Pantoea); the ensiled material contained mostly sequences associated with the lactic acid bacteria (LAB) (including the genera: Lactobacillus, Pediococcus and Lactococcus). Exposure to air resulted in even greater percentages of LAB, especially among the genus Lactobacillus, and a significant drop in bacterial diversity. Conclusions In-depth 16S rRNA gene sequence analysis revealed significant bacterial population structure changes during ensiling and again during exposure to air. Significance and Impact of the Study Significance and Impact of the Study: This in-depth description of the bacterial population dynamics that occurred during ensiling and simulated feed out expands our knowledge of these processes.
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