Benzaldehyde, the simplest aromatic aldehyde, is one of the most wide-spread volatiles that serves as a pollinator attractant, flavor, and antifungal compound. However, the enzyme responsible for its ...formation in plants remains unknown. Using a combination of in vivo stable isotope labeling, classical biochemical, proteomics and genetic approaches, we show that in petunia benzaldehyde is synthesized via the β-oxidative pathway in peroxisomes by a heterodimeric enzyme consisting of α and β subunits, which belong to the NAD(P)-binding Rossmann-fold superfamily. Both subunits are alone catalytically inactive but, when mixed in equal amounts, form an active enzyme, which exhibits strict substrate specificity towards benzoyl-CoA and uses NADPH as a cofactor. Alpha subunits can form functional heterodimers with phylogenetically distant β subunits, but not all β subunits partner with α subunits, at least in Arabidopsis. Analysis of spatial, developmental and rhythmic expression of genes encoding α and β subunits revealed that expression of the gene for the α subunit likely plays a key role in regulating benzaldehyde biosynthesis.
The flowability and mechanical properties are increasingly crucial in the filling process of deep metal mines with mining depths exceeding 1000 m. The rheological properties of filling slurry in the ...pipeline were analyzed through rheological tests, L-tube self-flow tests, and semi-industrial loop tests. The results revealed that with an increase in the cement-to-tailings mass ratio (c/t ratio) and mass concentration, the slurry exhibited a higher flow resistance and decreased stowing gradient. During slurry transportation, the pressure loss in the straight pipe was positively correlated with the slurry flow rate, c/t ratio, and mass concentration. A uniaxial compressive strength (UCS) test was conducted to analyze the mechanical properties of the cemented paste backfill containing BMC (CCPB) in both standard and deep-underground curing environments. The UCS of the CCPB showed an increasing trend with the rise in curing age, mass concentration, and the c/t ratio. The comprehensive analysis concluded that when the c/t ratio is 1:4, and the mass concentration is approximately 74%, and parameters such as the slump, bleeding rate, and flowability of the filling slurry meet the criteria for conveying and goaf filling, resulting in a high-strength filling body.
In this paper, the internal pore structure and mechanical properties of cemented paste backfill (CPB) with different mix proportions are studied by low field nuclear magnetic resonance (NMR) and ...unconfined compressive strength (UCS) test, and the relationship between UCS, pore structure characteristics and curing time is quantitatively characterized. The following conclusions are obtained: (1) The internal pores of CPB are divided into micropores, secondary pores, and macropores, among which secondary pores account for the largest proportion, accounting for about 70% of the total pores. The proportion of macropores is inversely proportional to the UCS, while the proportion of micropores is proportional to the UCS. (2) The porosity of CPB decreases with the increase of curing time, and UCS increases with the increase of curing time. The curing time has an exponential function relationship with porosity. UCS decreases exponentially with the increase of porosity. (3) A damage model of CPB is established considering the initial pore damage, which is highly consistent with the experimental results. The total damage evolution curve presents an “S" type distribution. The total damage change rate evolution curve showed a normal distribution.
The concepts of initial damage, loaded damage, and total damage were introduced for cemented paste backfills (CPBs) with stratified structures. Based on these, the damage evolution model, damage ...constitutive model, and strength criterion of CPB with stratified structures were formulated based on damage theory and the total differential rule. Finally, compression experiments and numerical simulations of CPBs were conducted with three-dimensional particle flow code with the layer numbers of 0, 1, 2, and 3, a solid content of 75% and a cement–sand ratio of 1:4. The theoretical curves of the damage constitutive model and strength criterion of the layered CPB closely matched the curves generated based on experimental tests and numerical simulations. This finding verified the correctness of the model. The initial increase of the damage fits closely to a quadratic polynomial as a function of the layer number, the total damage rate first increased and then decreased, while the maximum of the total damage rate increased.
Podoplanin (PDPN) expressed on tumour cells interacts with platelet C-type lectin-like receptor 2 (CLEC-2). This study aimed to investigate the role of the PDPN-platelet CLEC-2 interaction in ...melanoma pulmonary metastasis.
Murine melanoma B16-F0 cells, which have two populations that express podoplanin, were sorted by FACS with anti-podoplanin staining to obtain purified PDPN + and PDPN- B16-F0 cells. C57BL/6J mice transplanted with CLEC-2-deficient bone marrow cells were used for in vivo experiments.
The in vivo data showed that the number of metastatic lung nodules in WT mice injected with PDPN + cells was significantly higher than that in WT mice injected with PDPN- cells and in WT or CLEC-2 KO mice injected with PDPN- cells. In addition, our results revealed that the platelet Syk-dependent signalling pathway contributed to platelet aggregation and melanoma metastasis.
Our study indicates that the PDPN-CLEC-2 interaction promotes experimental pulmonary metastasis in a mouse melanoma model. Tumour cell-induced platelet aggregation mediated by the interaction between PDPN and CLEC-2 is a key factor in melanoma pulmonary metastasis.
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DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Reducing solid waste emission is the development direction of cleaner production in the future. This paper proposes to use the agricultural solid waste rice husk ash (RHA) as an alternative ...cementitious material for the underground filling of mines. The pore structure and mechanical properties of cemented paste backfill with different RHA dosages (RCPB) are analyzed. The results reveal that: (1) The pore structure of RCPB is divided into three types: micropores (<0.1 μm), secondary pores (0.1–100 μm), and main pores (>100 μm). The total number of micropores accounts for the largest proportion, followed by secondary pores and main pores. The total volume of secondary pores accounts for the largest proportion, followed by micropores and main pores. (2) The porosity decreases and the UCS increases with the increase of RHA dosage and curing time. The UCS decreases linearly with the increase of RHA dosage. The UCS has a linear negative correlation with porosity. (3) The cracks of RCPB initiated at the yield point, gradually expanded at the peak point, and finally penetrated at the post-peak point. The failure mode of RCPB is mainly a tensile failure, accompanied by a small number of secondary tensile cracks. (4) The hydration products of RCPB are mainly C–S–H and AFt gels. The greater the RHA dosage and CT, the more hydration products, the fewer pores, the greater the strength, and the greater the average gray value. The outcome of this work will afford a new ideas and methods for using solid waste (Rice husk) as backfilling.
The goaf formed by mining is filled and treated, which greatly improves the safety and stability of the surrounding rock. During the filling process, the roof-contacted filling rates (RCFR) of goaf ...were closely related to the stability control of the surrounding rock. The influence of the roof-contacted filling rate on the mechanical characteristics and crack propagation of the goaf surrounding rock (GSR) has been studied. Biaxial compression experiments and numerical simulation experiments were conducted on samples under different operating conditions. The results were as follows: (1) The peak stress, peak strain, and elastic modulus of the GSR are closely related to the RCFR and the goaf size; they increase with the increase of the RCFR, and decrease with the increase of the goaf size; (2) In the initial loading stage, a small number of cracks are generated, and the acoustic emission ringing count increases slowly. The mid-loading stage is the crack initiation and rapid expansion, and the cumulative ring count curve shows a "stepwise" growth. In the later loading stage, cracks continue to propagate and form macroscopic fractures, but the number of rings significantly decreases; (3) Shear cracks are prone to occur in the rock part of the GSR; tensile cracks are prone to occur in the backfill; and the crack propagation speed in the rock is faster than in the backfill. Stress concentration is the direct cause of GSR failure. The maximum concentrated stress of rock mass and backfill is 1~2.5 times and 0.17~0.7 times of the peak stress of the GSR, respectively.
Core 1-derived mucin-type O-glycans (O-glycans) are a major component of gastric mucus with an unclear role. To address this, we generated mice lacking gastric epithelial O-glycans (GEC C1galt1-/-). ...GEC C1galt1-/- mice exhibited spontaneous gastritis that progressed to adenocarcinoma with ∼80% penetrance by 1 yr. GEC C1galt1-/- gastric epithelium exhibited defective expression of a major mucus forming O-glycoprotein Muc5AC relative to WT controls, which was associated with impaired gastric acid homeostasis. Inflammation and tumorigenesis in GEC C1galt1-/- stomach were concurrent with activation of caspases 1 and 11 (Casp1/11)-dependent inflammasome. GEC C1galt1-/- mice genetically lacking Casp1/11 had reduced gastritis and gastric cancer progression. Notably, expression of Tn antigen, a truncated form of O-glycan, and CASP1 activation was associated with tumor progression in gastric cancer patients. These results reveal a critical role of O-glycosylation in gastric homeostasis and the protection of the gastric mucosa from Casp1-mediated gastric inflammation and cancer.
Quantitative real-time PCR (RT-qPCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Several studies examining ...the selection of reference genes have been performed in ornamental plants but none in sweet osmanthus (Osmanthus fragrans Lour.). Based on transcriptomic sequencing data from O. fragrans buds at four developmental stages, six reference genes (OfACT, OfEF1α, OfIDH, OfRAN1, OfTUB, and OfUBC2) with stable expression (0.5 to 2 fold change in expression levels between any two developmental stages), as well as the commonly used reference gene Of18S, were selected as candidates for gene expression normalization in the RT-qPCR analysis of O. fragrans. For the normalization of RT-qPCR with two dyes, SYBR Green and EvaGreen, the expressional stability of seven candidate reference genes in 43 O. fragrans samples was analyzed using geNorm, NormFinder and BestKeeper. For RT-qPCR using SYBR Green, OfRAN1 and OfUBC2 were the optimal reference genes for all samples and different cultivars, OfACT and OfEF1α were suitable for different floral developmental stages, and OfACT was the optimal reference gene for different temperature treatments. The geometric mean values of the optimal reference gene pairs for the normalization of RT-qPCR are recommended to be used for all samples, different cultivars and different floral developmental stages in O. fragrans. For RT-qPCR using EvaGreen, OfUBC2 was the optimal reference gene for all samples and different cultivars, and OfACT was the optimal reference gene for different floral developmental stages and different temperature treatments. As the worst reference gene, Of18S should not be used as a reference gene in O. fragrans in the future. Our results provide a reference gene application guideline for O. fragrans gene expression characterization using RT-qPCR.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK