Background
The relationship between obesity and risk of complications described during the 2009 influenza pandemic is poorly defined for seasonal influenza and other viral causes of influenza‐like ...illness (ILI).
Methods
An observational cohort of hospitalized and outpatient participants with ILI was conducted in six hospitals in Mexico. Nasopharyngeal swabs were tested for influenza and other common respiratory pathogens.
Results
A total of 4778 participants were enrolled in this study and had complete data. A total of 2053 (43.0%) had severe ILI. Seven hundred and seventy‐eight (16.3%) were positive for influenza, 2636 (55.2%) were positive for other viral respiratory pathogens, and 1364 (28.5%) had no respiratory virus isolated. Adults with influenza were more likely to be hospitalized if they were underweight (OR: 5.20), obese (OR: 3.18), or morbidly obese (OR: 18.40) compared to normal‐weight adults. Obese adults with H1N1 had a sixfold increase in odds of hospitalization over H3N2 and B (obese OR: 8.96 vs 1.35, morbidly obese OR: 35.13 vs 5.58, respectively) compared to normal‐weight adults. In adults with coronavirus, metapneumovirus, parainfluenza, and rhinovirus, participants that were underweight (OR: 4.07) and morbidly obese (OR: 2.78) were more likely to be hospitalized as compared to normal‐weight adults. All‐cause influenza‐like illness had a similar but less pronounced association between underweight or morbidly obesity and hospitalization.
Conclusions
There is an increased risk of being hospitalized in adult participants that are underweight or morbidly obese, regardless of their viral pathogen status. Having influenza, however, significantly increases the odds of hospitalization in those who are underweight or morbidly obese.
•Ionic liquid is covalently immobilized on the surface of magnetic nanoparticles.•The IL-MNPs are employed to extract thirteen PAHs from water samples.•The extracted analytes are finally determined ...by GC/MS.
In this paper, ionic liquid coated magnetic nanoparticles (IL-MNPs) have been prepared by covalent immobilization. The as-synthesized MNPs have been successfully used as sorbent for the extraction of polycyclic aromatic hydrocarbons (PAHs) from water samples, the analytes being finally determined by gas chromatography/mass spectrometry. The influence of several experimental variables (including the ionic strength, amount of MNPs, sample volume, agitation time and desorption solvent) has been considered in depth in the optimization process. The developed method, which has been analytically characterized under its optimal operation conditions, allows the detection of the analytes in the samples with method detection limits in the range from 0.04μgL−1 (fluoranthene) to 1.11μgL−1 (indeno(1,2,3-cd)pyrene). The repeatability of the method, expressed as relative standard deviation (RSD, n=7), varies between 4.0% (benzobfluoranthene) and 8.9% (acenaphthene), while the enrichment factors are in the range from 49 (naphthalene) to 158 (fluoranthene). The proposed procedure has been applied for the determination of thirteen PAHs in water samples (tap, river, well and reservoir ones) with recoveries in the range from 75 to 102%.
Agricultural management decision-making in salinization-prone environments requires efficient soil salinity monitoring methods. This is the case in the B-XII irrigation district in SW Spain, a heavy ...clay reclaimed marsh area where a shallow saline water table and intensively irrigated agriculture create a fragile balance between salt accumulation and leaching in the root zone, which might be disrupted by the introduction of new crops and increasing climate variability. We evaluated the potential of electromagnetic induction (EMI) tomography for field-scale soil salinity assessment in this hyper-conductive environment, using EMI and limited analytical soil data measured in 2017 and 2020 under a processing tomato–cotton–sugar beet crop rotation. Salinity effects on crop development were assessed by comparing Sentinel 2 NDVI imagery with inverted depth-specific electrical conductivity (EC). Average apparent electrical conductivity (ECa) for the 1-m depth signal was 20% smaller in 2020 than in 2017, although the spatial ECa pattern was similar for both years. Inverted depth-specific EC showed a strong correlation (R ≈ 0.90) with saturated paste extract EC (ECe), Na+ and sodium absorption ratio (SAR), resulting in linear calibration equations with R2 ≈ 0.8 for both years and leave-one-out cross validation Nash–Sutcliffe Efficiency Coefficient, ranging from 0.57 to 0.74. Overall, the chemical parameter estimation improved with depth and soil wetness (2017), yielding 0.83 < R <0.98 at 0.9 m. The observed spatial EC distributions showed a steadily increasing inverse correlation with NDVI during the growing season, particularly for processing tomato and cotton, reaching R values of −0.71 and −0.85, respectively. These results confirm the potential of EMI tomography for mapping and monitoring soil salinity in the B-XII irrigation district, while it allows, in combination with NDVI imagery, a detailed spatial assessment of soil salinity impacts on crop development throughout the growing season. Contrary to the popular belief among farmers in the area, and despite non-saline topsoil conditions, spatial EC and subsoil salinity patterns were found to affect crop development negatively in the studied field.
The implementation of soil conservation measures is essential to promote sustainable crop production in the Mediterranean region. In an organic rainfed almond orchard located in Lanjarón (SE, Spain), ...a study carried out during 2016–2021 analyzed the influence of different soil management strategies (SMSs) (TT, traditional tillage; NT, no tillage; VF, cover of Vicia faba; VS, cover of Vicia sativa; VS-VE, cover of Vicia sativa and Vicia ervilia) on some selected physical (bulk density, available water content, and aggregate stability), chemical (pH, electrical conductivity, soil-organic content, N, P, K, and micronutrients), and biological (microbial activity) soil properties, relevant to soil health, and their implications for yield and almond quality (physical and chemical). Our results showed that the SMS with legume cover improves soil properties, which had a favorable effect on soil health. The mean almond yield was not significantly affected by the SMS applied, being 315.9, 256.4, 229.1, 212.5, and 176.6 kg ha−1 year−1 for TT, VF, VS-VE, VS, and NT, respectively. Regarding the almond nut quality, the strategy based on implementation of legume cover increased the almond antioxidant activity and the total polyphenol content, which would improve their nutritional value. Here we showed how the use of sustainable SMSs improved the soil properties compared to traditional tillage in rainfed organic almonds, allowing the long-term sustainability of agroecosystems while at the same time obtaining higher nutritional quality almonds.
En España, hay un interés creciente por la conservación de las razas caprinas autóctonas con formas de producción basadas en el pastoreo y por la posibilidad de transformación en producciones ...ecológicas. El objetivo ha sido evaluar las características sensoriales de la carne de cabrito lechal de dos razas autóctonas, criados en sistemas de producción convencional y ecológica, basados en el pastoreo. Se utilizaron 21 cabritos lechales de los cuales 12 fueron criados en un sistema ecológico (6 de raza Payoya y 6 de raza Blanca Andaluza) y 9 en un sistema convencional (3 de raza Payoya y 6 de raza Blanca Andaluza). El perfil sensorial de la carne se evaluó mediante un panel analítico. Con relación al sistema de explotación, los resultados obtenidos indican que las carnes procedentes de sistemas ecológicos tenían menos intensidad de olor y una textura más blanda, tierna y jugosa que las carnes del sistema convencional. Respecto a la raza, el estudio mostró que las carnes de cabrito de la raza Blanca Andaluza presentaron una intensidad de olor menor y una textura más blanda, tierna y jugosa que las carnes de cabrito de la raza Payoya. Estos resultados preliminares podrían ser favorables para la transformación de las explotaciones convencionales, basadas en pastoreo, a ecológicas.
Con ocasión del 80 aniversario de la Universidad Iberoamericana y de su Departamento de Filosofía hemos querido recuperar y comentar uno de los dos primeros libros que se publicaron en lo que ...entonces se llamaba Centro Cultural Universitario. El libro que nos ocupa es fiel testimonio de la época en que fue publicado. Las conferencias del padre jesuita Ledit son ejemplo de la manera de entender la historia moderna y contemporánea de muchos pensadores católicos preconciliares. La cristiandad medieval había realizado una síntesis con el pensamiento griego. La modernidad fue la paulatina separación de esta filosofía perenne abierta a la trascendencia, con el resultado de una razón soberbia y orgullosa de su finitud; una de sus consecuencias fueron los sistemas totalitarios de la primera mitad del siglo xx, los cuales en su confrontación llevaron al estallido de la Segunda Guerra Mundial.
(1) Introduction. An analysis was made of posterior crossbites in deciduous dentition and their relation to the type of feeding received by the child, with the objective of determining the influence ...of the way in which the child is fed in the early stages of life on the development of posterior crossbites. (2) Material and methods. A total of 1401 preschool children between 3 and 6 years of age from Seville (Spain) were included in the study. An intraoral exploration was carried out to assess the presence of crossbites (uni- or bilateral, and functional or not). The study was completed with a parent or legal guardian questionnaire exploring the type of feeding received by the child in the first stages of life, as well as the presence of bad oral habits and their duration. (3) Results. A total of 276 children (19.7%) presented posterior crossbite in occlusion. Uponn centering the midlines, 197 were maintained, indicating that 79 were due to premature contacts (functional crossbites). There were no significant differences in crossbites among the children who had received breastfeeding, though bottle-feeding was seen to favor crossbite. (4) Conclusions. No statistically significant relationship was found between posterior crossbites and breastfeeding, though an association between posterior crossbites and bottle-feeding was observed, with the number of crossbites increasing with the duration of bottle-feeding.
The need to improve agroecosystem sustainability to secure yields, minimize environmental impacts and improve soil health is widely recognized. Organic production systems are one of the strategies ...that may be used to alleviate the negative environmental repercussions of conventional agriculture. In the present study, we compared the impact of conventional and organic production systems on the almond (Prunus dulcis (Mill.) D.A. Webb) yield and quality of nuts of two cultivars (Marcona and Desmayo largueta), with both systems being managed on marginal hillslopes in the southeast of Spain. Our findings show that the organic production system in rainfed almond trees has positive effects on certain nut quality parameters, with a slight decrease in almond yield, specifically 9.5% for cv. D. largueta and 1.3% for cv. Marcona, with respect to the conventional system. The results obtained have varied depending on the cultivar. Statistically significant differences have been obtained for cv. Marcona in the sugar content (54.4 and 49.8 g kg−1 in organic and conventional, respectively) and the total phenol content (3.41 and 2.46 g GAE kg−1 for organic and conventional, respectively). In the case of cv D. largueta, statistically significant differences were found between the organic and conventional systems for antioxidant activity (14.8 vs. 8.68 mmol Trolox kg−1, DPPH), fatty acid content (229 vs. 188 g kg−1 dw), saturated fatty acids (36 vs. 28.7 g kg−1 dw), monounsaturated fatty acids (113 vs. 110 g kg−1 dw) and polyunsaturated fatty acids (60.3 vs. 49.6 g kg−1 dw). Here, we show for the first time how a rainfed organic system allows for higher-quality almonds, specifically with a higher content of phytochemicals beneficial for health, which, together with the higher price compared to conventional almonds, could compensate for the yield losses while preserving the sustainability of marginal agroecosystems.
Objective To evaluate the use of multiple displacement amplification (MDA) for whole genome amplification in the preimplantation genetic diagnosis (PGD) of X-linked adrenoleukodystrophy. Design MDA ...was used to amplify the whole genome directly from a single blastomere. MDA products were used for polymerase chain reaction (PCR) analysis of two polymorphic markers flanking the ABCD1 gene and a new X/Y marker, X22, to sex embryos in an X-linked adrenoleukodystrophy PGD program. Setting Fertility and gynecology private center in Alicante, Spain. Patient(s) A couple in which the wife is a carrier of the ABCD1 gene mutation (676A→C) that was previously identified in her family. Intervention(s) MDA of single blastomere and PCR tests for PGD. Main Outcome Measure(s) The ability to analyze single blastomeres for X-linked adrenoleukodystrophy using MDA. Result(s) The development of an MDA-PGD protocol for X-linked adrenoleukodystrophy allowed for the diagnosis of five embryos. These were biopsied on day 3 of culture and analyzed. One embryo was an affected male and one embryo was a female carrier. Three healthy female embryos were transferred 48 hours after biopsy. Unfortunately, no pregnancy was achieved. Conclusion(s) The MDA technique is useful for overcoming the problem of insufficient genomic DNA in PGD and allows the simultaneous amplification of different targets to perform a diagnosis of any known gene defect and a sexing test by standard methods and conditions.
Background
αB‐crystallin is a promiscuous protein involved in numerous cell functions. Mutations in CRYAB have been found in patients with different pathological phenotypes that are not properly ...understood. Patients can present different diseases like cataracts, muscle weakness, myopathy, cardiomyopathy, respiratory insufficiency or dysphagia, but also a variable combination of these pathologies has been found. These mutations can show either autosomal dominant or recessive mode of inheritance and variable penetrance and expressivity. This is the first report of congenital cataracts and myopathy described in childhood due to a CRYAB mutation with autosomal dominant mode of inheritance.
Methods
The whole exome sequence was subjected to phenotype‐driven analysis and a novel variant in CRYAB was detected: c.514delG, p.(Ala172ProfsTer14). The mutation was located in the C‐terminal domain of the protein, which is essential for chaperone activity. The deduced protein was analyzed searching for alterations of the relevant physico‐chemical properties described for this domain. A muscle biopsy was also tested for CRYAB with immunohistochemical and histoenzymatic techniques.
Results
CRYAB displayed a mild immunoreactivity in the subsarcolemmal compartment with no pathological sarcoplasmic accumulation. It agrees with an alteration of the physico‐chemical properties predicted for the C‐terminal domain: hydrophobicity, stiffness, and isomerization.
Conclusions
The described mutation leads to elongation of the protein at the carboxi‐terminal domain (CTD) with altered properties, which are essential for solubility and activity. It suggests that can be the cause of the severe conditions observed in this patient.
The isolation of DNA and whole exome sequencing led us to discover a novel mutation in a CRYAB which causes cataracts, myopathies and other conditions observed in the proband. The predicted altered physico‐chemical properties of the C‐terminal are related with low activity and low solubility. This new condition agrees with the muscle biopsy where the cell is depleted and the mutated protein is found attached to the membrane cell.
PDF
