This study evaluated the effects of two chemical additives or a microbial inoculant on chemical composition and DM losses in whole-plant soybean silage. One-hundred and twenty mini-silos were used in ...a completely randomized design experiment with the following treatments: water without chloride (control, CON); a microbial inoculant (INO); a chemical additive containing 35–45% formic acid (FA type); and another chemical additive containing 50–60% propionic acid (PA type). Data were analyzed using mixed models of SAS, and treatment differences were evaluated by the following orthogonal contrasts: C1 = CON vs. additives (INO + FA type + PA type); C2 = INO vs. chemical additives (FA type + PA type); and C3 = PA type vs. FA type. Silage pH and ammonia nitrogen concentration were decreased, and concentrations of lactic acid and acetic acid were increased with additives. Counts of lactic acid bacteria were higher in silages with INO than with chemical additives. DM recovery increased with FA type and PA type. Additives increased DM and CP concentrations. Silage A-fraction proportion was greater with additives. Additives, particularly FA type and PA type, improved chemical composition and fermentative profile and reduced undigestible proportions of protein in whole-plant soybean silage. Chemical additives were more effective in reducing silage DM losses than INO.
ABSTRACT Aimed with this study was evaluate the increasing levels of crambe crushed, on in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM) and crude protein (IVDCP), parameters of ...fermentation and kinetics of the cumulative gas production. The crushed crambe were included in the diets at 0; 50; 100; 150 g/kg of DM. There was a quadratic effect of the proportions of crambe crushed in the concentrated supplements on IVDDM and IVDOM (maximum point at 0.25 and 0.91 g Kg-1 DM, respectively). Total gas production (A + D) was influenced by the presence of crambe crushed with quadratic effect on minimal point on 0.02 g Kg-1 DM. There was a quadratic effect for pH in the collection times in all diets, the lowest pH values were observed between 4.8 to 8.7 hours. There was a quadratic effect for ammoniacal nitrogen in the collection times in all diets with peaks of NH3-N production occurred between 4.8 and 6.3 hours after the beginning of the incubation. Concluded that the inclusion of crambe crushed up to 150 g kg-1 DM in diets for ruminants does not impair the ruminal degradation kinetics.
RESUMO O objetivo deste estudo foi avaliar a inclusão de níveis crescentes de torta de crambe, sobre a digestibilidade in vitro da matéria seca (DIVMS), matéria orgânica (DIVMO) e proteína bruta, cinética da produção cumulativa de gás e parâmetros de fermentação. Foram avaliadas as inclusões de 0; 50; 100; 150 g / kg de MS em dietas. Houve efeito quadrático das proporções de torta de crambe nos suplementos concentrados para DIVMS e DIVMO (ponto máximo em 0,25 e 0,91 g Kg-1 DM, respectivamente). A produção total de gás (A + D) foi influenciada por a presença de torta de crambe com efeito quadrático tendo como ponto mínimo em 0,02 g kg-1 DM. Houve um efeito quadrático do pH e NH3-N nos tempos de coleta em todas as dietas, os menores valores de pH foram observados entre 4,8 a 8,7 e os picos de produção de NH3-N entre 4,8 e 6,3 horas após o início da incubação. Conclui-se que a substituição do farelo de soja por crambe triturado até 150 g kg-1 em suplementos concentrados para ruminantes não prejudica a cinética de degradação ruminal.
Fiber digestibility is a key-point of forage usage in ruminant production systems. The present study aimed to evaluate the effect of fibrolytic enzyme blend on whole-plant sorghum silage fermentation ...profile, fermentative losses, chemical composition,
degradation, and aerobic stability. It used fifty experimental silos (plastic bucket, 28 cm i.d. and 25 cm in height) in a blocked randomized design to evaluate the following fibrolytic enzyme levels: 0, 150, 300, 450, and 600 mL per ton of dry matter (DM). Enzymes had 300 U/mL of xylanase and 300 U/mL of cellulase. Enzymes linearly increased acetic acid, branched-chain organic acids, and ethanol concentration of silage. Although there was no treatment effect on silage DM recovery over the ensiling process, enzyme levels linearly increased gas, effluent, and total losses. Intermediary levels of enzymes (from 312 to 342 mL/ton DM) decreased fiber content (NDF and ADF), whereas improved NFC silage content. Besides, enzymes did not affect DM and NDF degradation and tended to linearly reduce acid detergent fiber
degradation. The enzymes addition linearly decreased silage temperature after aerobic exposure. However, there was no treatment effect on silage pH after aerobic exposure also during the time that silage remained with a temperature lower than 2°C above environmental temperature. Thus, fibrolytic enzymes reduce fiber content, promote a heterolactic fermentation, and reduce silage temperature after aerobic exposure. However, this increases fermentative losses and has no positive effect on
degradation and the time of aerobic stability.
This paper aims to investigate the influence of increasing chitosan doses on the relative proportion and abundance of cellulotytic, amylolytic bacteria, and Archaea transcripts for grazing cattle. ...Five rumen cannulated crossbread steers 3.6 months and 300 ± 25 kg body LW (live weight), mean ± standard deviation were used in a 5 × 5 latin square design, randomly assigned to treatment sequence containing chitosan added to 0, 400, 800, 1200, or 1600 mg/kg concentrate. There was the effect of chitosan on the population of Fibrobacter succinogenes, Ruminococcus albus, and Archaea. The lowest population of these bacteria of 576.60 mg/kg DM (dry matter), 1010.40 mg/kg DM, and 634.80 mg/kg DM were noted when chitosan was added at levels of 3.87, 4.16, and 3.52. Except for Ruminococcus albus, which was not affected by increasing chitosan doses, supplementation of this additive in the concentrate quadratically increased the relative abundance of Fibrobacter succinogenes and Archaea Supplemental 740 mg CHI/kg concentrate for grazing steers receiving concentrate at 150 grams/100 kg LW is recommended to promote minimal effect on the relative population and abundance of cellulolytics and amylomatics and to restrict Archaea growth.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, GIS, IJS, IZUM, KILJ, KISLJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
ABSTRACT This study was undertaken to determine the effects of chitosan and whole raw soybean on nutrient intake and total tract digestion, nitrogen utilization, microbial protein synthesis, blood ...metabolites, and energy balance of dairy heifers. Twelve Jersey heifers (6±0.5 months of age and 139.50±25.56 kg of live weight; mean ± standard deviation) were randomly assigned to a replicated Latin square design with a 2 × 2 factorial arrangement. The experimental period consisted of 14 days of adaptation to diets, six days of sampling, and five days of washout. The experimental diets were: control (CO); chitosan (CHI, inclusion of 2.0 g kg−1 DM of chitosan); whole raw soybean (WS, 163.0 g kg−1 of WS on diet DM basis); and chitosan + whole raw soybean (CHI+WS). Chitosan decreased dry matter and neutral detergent fiber intakes; however, CHI increased DM total tract digestion. An interaction effect was observed on retained nitrogen, which increased when animals were fed CHI+WS compared with CO or CHI, but did not differ from that of animals fed WS. Chitosan decreased microbial nitrogen and crude protein flow of heifers. Energy balance was improved when heifers received diets containing WS. Efficiency of energy utilization was not affected by experimental diets. An interaction effect was observed for blood high-density lipoprotein (HDL) concentration, which increased with both dietary inclusion of CHI and WS compared with the other diets, and CHI provided the lowest value of HDL cholesterol. Chitosan and whole raw soybean do not alter nutrient intake and total tract digestion; however, they decrease nitrogen urinary excretion and increase blood HDL cholesterol of heifers.
The aim of this study was to evaluate the haematological, biochemical and body temperature parameters of pacu Piaractus mesopotamicus juveniles after capture stress followed by anaesthetic induction. ...Fish (30.4±1.4 g) were divided into five groups (12 fish each group): control (water only handling), ethanol handling (600 µL L-1), handling with 100, 350 and 600 mg L-1 of essential Ocimum basilicum oil. Fish were caught and anaesthetised, followed by biometric handling, blood collection and thermographic images. Increased anaesthetic concentrations had a linear positive effect on haemoglobin content, erythrocytes, mean corpuscular haemoglobin concentration (MCHC), monocytes and granular leukocyte PAS positive (LG-PAS) (P<0.05). A quadratic effect (P<0.05) was observed for lymphocytes, with a maximum peak at the 350 mg L-1 concentration. The fish surface temperature ranged was 25.9-29.9 ºC, with the highest values in the non-anaesthetised fish’s cephalic regions. The fish anaesthetised with the O. basilicum essential oil 100 mg L-1 concentration showed a lower surface temperature. Using O. basilicum essential oil in biometric handling procedures was unable to prevent stress-related haematological alterations in juvenile pacu. Employing infrared thermography to assess surface temperature provides useful data to understand the effects of anaesthesia on fish, but more studies are needed to better understand this technique as a measure of well-being in fish farming.
The purpose of this study was to evaluate the effects of inoculation of Lactobacillus buchneri (L. buchneri) alone or with Bacillus subtilis (B. subtilis) on gas and effluent losses, chemical ...composition, in vitro digestibility of nutrients, aerobic stability, and microbiological quality of sunflower silage. A randomized experimental design was used, which contained 3 treatments, each one included 15 mini-silos. Mini-silos were distributed to the following treatments: (1) Control (CON), without inoculants; (2) L. buchneri alone (LB), inoculation at 2.6 × 10
10
cfu/g; and (3) L. buchneri with B. subtilis (LB + BS), inoculation at 2.6 × 10
10
cfu/g and 1 × 10
9
cfu/g with L. buchneri and B. subtilis, respectively. Treatments were applied at 2 g/t of fresh forage ensiled. Silages with microbial inoculants had lower DM content, and higher in vitro digestibility of DM and neutral detergent fibre than CON. Inoculants decreased the number of aerobic bacteria, mould, and yeast, and increased the counts of lactic acid bacteria in sunflower silage. L. buchneri exhibited positive effects on aerobic stability, in vitro digestibility of nutrients and decreased the counts of mould and yeast, but did not show a synergistic effect with B. subtilis on sunflower silage.
This study aimed to evaluate the effects of the inclusion of unsaturated fatty acid (UFA) sources on the nutrient intake, apparent digestibility, ruminal fermentation, and feeding behavior in diets ...for buffaloes. Four castrated Murrah buffaloes with approximately 24 months of age and an initial average body weight of 351 ± 15 kg were randomly assigned to a 4 × 4 Latin square experiment, containing the following diets: (1) control (CON): control diet based on soybean meal and ground corn, (2) soybean oil (SO): dietary inclusion of 2.20% (DM basis), (3) whole raw soybean (WRS): dietary inclusion of 16.0%, and (4) calcium salts of FA (CSFA): dietary inclusion of 2.60%. There was an effect of diets in ether extract intake among buffaloes fed UFA and CON diets, and among buffaloes fed CSFA and WRS diets (
P
< 0.05). Diets containing UFA sources provided higher EE digestibility (
P
< 0.05). Buffaloes fed WRS had higher rumen pH values than animals fed the CSFA diet (
P
< 0.05). Supplementation of UFA sources decreased the molar concentrations of short-chain fatty acids (
P
< 0.05). Diets influenced the times spent in chewing, idling, and the rumination efficiencies of DM and NDF (
P
< 0.05). The supplementation with WRS, SO, and CSFA does not negatively affect intake, digestion, ruminal metabolism, and feeding behavior. The WRS as a fat supplement source decreases dietary costs by replacing ground corn and soybean meal simultaneously compared to other fat sources used. Nevertheless, whole and raw soybean in buffaloes’ diet can reduce chewing and rumination activity.
This work aims to provide an overview of the territorial evolution of COVID-19 (SARS-CoV-2) in Brazil using socio-demographic variables, for the time span between February 26, 2020 until January 24, ...2021. Socio-demographic indicators, basic sanitation infrastructure data, and epidemiological bulletins were integrated using Principal Components Analysis (PCA) to develop a social vulnerability index (SVI), to estimate the degree of exposure risk of the Brazilian population to COVID-19. The results indicate that the majority of confirmed cases were reported from the main Brazilian capitals, linked to well-developed port and airport modes. In terms of deaths, the states of São Paulo, Rio de Janeiro, Ceará and Pernambuco were at the top of the ranking. On the contrary, there were some states of the mid-west (Mato Grosso do Sul) and the north (Acre, Amapá, Roraima, Rondônia and Tocantins), that recorded low mortality indexes. The SVI reveals that the states of the north and north-east are the most vulnerable. Regarding the metropolitan areas, it was observed that the main capitals of the north and north-east, with the exception of Salvador, present significantly more critical numbers in terms of dissemination and deaths by COVID-19 than the capitals of the south-southeast, where the SVI is lower. The comparative exception was Santa Catarina state metropolitan areas. Finally, as the virus does not strike everyone in the same way, one of the great challenges is to search for solutions to cope with COVID-19 in the face of very unequal realities. Thus, a reflection on the strategies adopted by the Brazilian government is relevant, while considering the continental dimensions and the diversity of the Brazilian regions, to obtain a better analysis of the more vulnerable populations and social groups.
The objective of this work was to evaluate the performance of the thermopotassium and thermophosphate agrominerals, as sources of P and K, in an organic pasture consortium of 'Marandu' Urochloa ...brizantha + Stylosanthes spp. in the Brazilian Cerrado. The organic management was compared with conventional farming using chemical fertilizers, during three years, in two adjacent experiments, one with and the other without previous growth of green manure (Crotalaria juncea). The management treatments were: conventional, with the application of 200 kg ha-1 potassium chloride, 260 kg ha-1 triple superphosphate, and 217 kg ha-1 urea; organic, with the application of 1.0 Mg ha-1 thermophosphate, 2.0 Mg ha-1 termopotassium, and 7.0 Mg ha-1 chicken manure; and control, without fertilizers. Soil fertility, dry mass production, and legume content in the pasture were evaluated. The experiment with green manure resulted in a significant increase in the soil-extractable contents of P and K and in pasture productivity. In the organic treatment, due to the slow release of agromineral nutrients, long-term effects are observed on the soil-extractable contents of P and K.
Resumo: O objetivo deste trabalho foi avaliar o desempenho dos agrominerais termopotássio e termofosfato, como fontes de P e K, em consórcio de pastagem orgânica de Urochloa brizantha 'Marandu' + Stylosanthes spp. no Cerrado brasileiro. O manejo orgânico foi comparado ao convencional com uso de fertilizantes químicos, durante três anos, em dois experimentos adjacentes, um com e outro sem cultivo prévio de adubo verde (Crotalaria juncea). Os tratamentos de manejo foram: convencional, com aplicação de 200 kg ha-1 de cloreto de potássio, 260 kg ha-1 de superfosfato triplo e 217 kg ha-1 de ureia; orgânico, com aplicação de 1,0 Mg ha-1 termopotássio, 2,0 Mg ha-1 termofosfato e 7,0 Mg ha-1 cama de frango; e controle, sem adubação. Foram avaliadas fertilidade do solo, produção de matéria seca e contribuição da leguminosa na pastagem. O experimento com adubação verde proporcionou aumento significativo nos teores de P e K extraíveis do solo e na produtividade da pastagem. No tratamento orgânico, devido à liberação lenta dos nutrientes dos agrominerais, são observados efeitos de longo prazo nos teores extraíveis de P e K do solo.
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