Book Reviews Kasioumi, Eirini; Plyushteva, Anna; Zemach-Bersin, Talya ...
03/2018, Letnik:
8, Številka:
1
Book Review
Recenzirano
Max Hirsh, Airport Urbanism: Infrastructure and Mobility in Asia (Minneapolis: University of Minnesota Press, 2016), 216 pp., 80 black-and-white illustrations, 20 color plates, $25 (paperback), ...$87.50 (hardback)Laura Bang Lindegaard, Congestion: Rationalising Automobility in the Face of Climate Change (Frankfurt: Peter Lang, 2015), 214 pp., $54.95 (hardback)Neriko Musha Doerr and Hannah Davis Taïeb, eds., The Romance of Crossing Borders: Studying and Volunteering Abroad (New York: Berghahn Books, 2017), 302 pp., $90 (hardback)Ehren Helmut Pflugfelder, Communicating Mobility and Technology: A Material Rhetoric for Persuasive Transportation (London: Routledge, 2017), 178 pp., 19 illustrations, $149.95 (hardback), $54.95 (ebook)Christo Sims, Disruptive Fixation: School Reform and the Pitfalls of Techno- Idealism (Princeton, NJ: Princeton University Press, 2017), 232 pp., $27.95 (paperback), $80 (hardback)Charlotte Mathieson, ed., Sea Narratives: Cultural Responses to the Sea, 1600– Present (London: Palgrave Macmillan, 2016), 281 pp., 5 illustrations, €93.59 (hardback), €74.96 (ebook)Till Mostowlansky, Azan on the Moon: Entangling Modernity along Tajikistan’s Pamir Highway (Pittsburgh, PA: University of Pittsburgh Press, 2017), 240 pp., 25 black-and-white illustrations, $26.95 (paperback)Steff en Köhn, Mediating Mobility: Visual Anthropology in the Age of Migration (New York: Columbia University Press, 2016), 208 pp., $30 (paperback)Margaret Guroff, The Mechanical Horse: How the Bicycle Reshaped American Life (Austin: University of Texas Press, 2016), 295 pp., 10 black-and-white photographs, 5 black-and-white illustrations, $17.95 (paperback)Melody L. Hoffmann, Bike Lanes Are White Lanes: Bicycle Advocacy and Urban Planning (Lincoln: University of Nebraska Press, 2016), 210 pp., $40 (paperback)Alexander Braun, ed., Winsor McCay: The Airship Adventures of Little Nemo (Cologne: Taschen, 2017), 288 pp., $15 (hardback)
Book Reviews Kasioumi, Eirini; Plyushteva, Anna; Zemach-Bersin, Talya ...
Transfers,
03/2018, Letnik:
8, Številka:
1
Book Review
Recenzirano
Max Hirsh, Airport Urbanism: Infrastructure and Mobility in Asia (Minneapolis: University of Minnesota Press, 2016), 216 pp., 80 black-and-white illustrations, 20 color plates, $25 (paperback), ...$87.50 (hardback) Laura Bang Lindegaard, Congestion: Rationalising Automobility in the Face of Climate Change (Frankfurt: Peter Lang, 2015), 214 pp., $54.95 (hardback) Neriko Musha Doerr and Hannah Davis Taïeb, eds., The Romance of Crossing Borders: Studying and Volunteering Abroad (New York: Berghahn Books, 2017), 302 pp., $90 (hardback) Ehren Helmut Pflugfelder, Communicating Mobility and Technology: A Material Rhetoric for Persuasive Transportation (London: Routledge, 2017), 178 pp., 19 illustrations, $149.95 (hardback), $54.95 (ebook) Christo Sims, Disruptive Fixation: School Reform and the Pitfalls of Techno- Idealism (Princeton, NJ: Princeton University Press, 2017), 232 pp., $27.95 (paperback), $80 (hardback) Charlotte Mathieson, ed., Sea Narratives: Cultural Responses to the Sea, 1600– Present (London: Palgrave Macmillan, 2016), 281 pp., 5 illustrations, €93.59 (hardback), €74.96 (ebook) Till Mostowlansky, Azan on the Moon: Entangling Modernity along Tajikistan’s Pamir Highway (Pittsburgh, PA: University of Pittsburgh Press, 2017), 240 pp., 25 black-and-white illustrations, $26.95 (paperback) Steff en Köhn, Mediating Mobility: Visual Anthropology in the Age of Migration (New York: Columbia University Press, 2016), 208 pp., $30 (paperback) Margaret Guroff, The Mechanical Horse: How the Bicycle Reshaped American Life (Austin: University of Texas Press, 2016), 295 pp., 10 black-and-white photographs, 5 black-and-white illustrations, $17.95 (paperback) Melody L. Hoffmann, Bike Lanes Are White Lanes: Bicycle Advocacy and Urban Planning (Lincoln: University of Nebraska Press, 2016), 210 pp., $40 (paperback) Alexander Braun, ed., Winsor McCay: The Airship Adventures of Little Nemo (Cologne: Taschen, 2017), 288 pp., $15 (hardback)
Book Reviews Kasioumi, Eirini; Plyushteva, Anna; Zemach-Bersin, Talya ...
Transfers,
03/2018, Letnik:
8, Številka:
1
Book Review
Recenzirano
Max Hirsh, Airport Urbanism: Infrastructure and Mobility in Asia (Minneapolis:
University of Minnesota Press, 2016), 216 pp., 80 black-and-white illustrations,
20 color plates, $25 (paperback), ...$87.50 (hardback)
Laura Bang Lindegaard, Congestion: Rationalising Automobility in the Face
of Climate Change (Frankfurt: Peter Lang, 2015), 214 pp., $54.95 (hardback)
Neriko Musha Doerr and Hannah Davis Taïeb, eds., The Romance of Crossing
Borders: Studying and Volunteering Abroad (New York: Berghahn Books,
2017), 302 pp., $90 (hardback)
Ehren Helmut Pflugfelder, Communicating Mobility and Technology: A Material
Rhetoric for Persuasive Transportation (London: Routledge, 2017), 178
pp., 19 illustrations, $149.95 (hardback), $54.95 (ebook)
Christo Sims, Disruptive Fixation: School Reform and the Pitfalls of Techno-
Idealism (Princeton, NJ: Princeton University Press, 2017), 232 pp., $27.95
(paperback), $80 (hardback)
Charlotte Mathieson, ed., Sea Narratives: Cultural Responses to the Sea, 1600–
Present (London: Palgrave Macmillan, 2016), 281 pp., 5 illustrations, €93.59
(hardback), €74.96 (ebook)
Till Mostowlansky, Azan on the Moon: Entangling Modernity along Tajikistan’s
Pamir Highway (Pittsburgh, PA: University of Pittsburgh Press, 2017),
240 pp., 25 black-and-white illustrations, $26.95 (paperback)
Steff en Köhn, Mediating Mobility: Visual Anthropology in the Age of Migration
(New York: Columbia University Press, 2016), 208 pp., $30 (paperback)
Margaret Guroff, The Mechanical Horse: How the Bicycle Reshaped American
Life (Austin: University of Texas Press, 2016), 295 pp., 10 black-and-white photographs,
5 black-and-white illustrations, $17.95 (paperback)
Melody L. Hoffmann, Bike Lanes Are White Lanes: Bicycle Advocacy and
Urban Planning (Lincoln: University of Nebraska Press, 2016), 210 pp., $40
(paperback)
Alexander Braun, ed., Winsor McCay: The Airship Adventures of Little Nemo
(Cologne: Taschen, 2017), 288 pp., $15 (hardback)
Preemptive and therapeutic donor lymphocyte infusions (preDLI and tDLI) are widely used in relapsing and relapsed hematopoietic malignancies after allogeneic stem cell transplantation (alloSCT) to ...enhance the graft-versus-malignancy effect. However, in advanced myeloid malignancies, long-term survival after preDLI and tDLI remains low, reflecting our inability to master the double-edged sword of alloreactivity, balancing anti-neoplastic activity versus graft-versus-host disease (GvHD). We previously evaluated a quantitative PCR-based high-sensitivity chimerism (hs-chimerism) based on insertion/deletion polymorphisms instead of short tandem repeats, where increasing host chimerism in peripheral blood predicts relapse more than a month before clinical diagnosis, and declining host chimerism signals anti-host alloreactivity. Here we report 32 consecutive patients with advanced myeloid malignancies receiving preDLI or tDLI “navigated” by hs-chimerism (“navigated DLI”). We compared them to a historical cohort of 110 consecutive preDLI or tDLI recipients, prior to implementation of hs-chimerism at our institution (“controls”). Both groups were comparable regarding age, gender, conditioning, donor type, and time to DLI. With longer median follow-up of the navigated DLI group (8.5 versus 5 months), their landmark overall (64%) and disease-free survival (62%) at 2 years from first DLI compared favorably with controls (23% and 21%, respectively). Improved survival of navigated DLI was due to both reduced relapse incidence (38% versus 60%) and non-relapse mortality (17% versus 44%) at 2 years. Early relapse prediction by hs-chimerism allowed a preemptive approach in 28% of navigated DLI versus 7% in controls. Our results confirm hs-chimerism as a highly valuable tool for monitoring and steering immune interventions after alloSCT.
Megakaryopoiesis is controlled by a variety of hematopoietic growth factors in order to maintain a physiological level of circulating platelets. Thrombopoietin (TPO) is the main regulator of ...megakaryopoiesis modulating megakaryocyte differentiation, promoting endomitosis and proplatelet formation and as such supports the self-renewal and survival of hematopoietic stem cells. To allow proper proliferation and differentiation of different hematopoetic lineages, TPO signal transduction must be tightly regulated. Several mechanisms negatively modulating hematopoiesis and differentiation of the megakaryocytic lineage have previously been identified. Among those are suppressors cytokine signaling, protein phosphatases as well as a multitude of negative regulatory signaling pathways. However, one of the most effective mechanisms to permanently disable activated signaling proteins is by targeted degradation via lysosomes or proteasomes.
In this study, we investigated the mechanisms that regulate TPO-mediated MPL degradation in primary mouse cells. Previous studies have identified CBL as an E3 ligase responsible for the ubiquitination of MPL in cell lines. In order to determine the potential role of c-CBL in murine thrombopoiesis, we used Cre/loxP technology to specifically delete c-CBL in the megakaryocytic lineage. Mice expressing two floxed c-CBL alleles were crossed to mice expressing Cre recombinase under the control of the platelet factor 4 (PF4) promoter. This yielded progeny with the desired genotype of c-CBLfl/fl PF4-Cre (CBL ko) after two generations of breeding. The desired cohort exhibited a quantitative absence of c-CBL in megakaryocytes and platelets as assessed by western blotting compared with wild type C57/BL6 mice. The expression of CBL in other hematopoietic cells such as B cells, T cells, neutrophils, monocytes and dendritic cells remained unaffected in this conditional ko strain. The experimental cohort showed significantly higher numbers of megakaryocytes in the bone marrow and of platelets in the peripheral blood as compared to wild type mice (1.2 mio vs. 1.8 mio cells/µl, p<0.0001). In addition, the platelets from the mutant mouse strain were of significantly smaller size (43 vs. 38 fL, p=0.0022). To evaluate the role of c-CBL in mature megakaryocytes, total bone marrow was collected from 12 wk old CBL ko mice and grown in TPO-containing culture medium for 72 h. Megakaryocytes derived from the bone marrow of wild type mice served as controls. Mature megakaryocytes were eventually isolated on a BSA-density gradient. Subsequent Western Blot analysis revealed a significant reduction of MPL ubiquitination in the CBL ko mice as compared to wild type mice, thereby identifying c-CBL as a critical negative regulator of megakaryopoesis.
Taken together, we have successfully ablated c-CBL specifically from the megakaryocyte lineage and could demonstrate that this has profound effects on platelet counts and platelet size. In addition, we were able to show that c-CBL ablation leads to reduced ubiquitination of MPL and a consecutively longer half life of this protein culminating in substantially increased megakaryopoiesis in the c-CBL ko cohort. In summary, these data enhance our understanding of the regulation of TPO signaling and the physiological role of CBL in the megakaryocytic lineage.
No relevant conflicts of interest to declare.
Human cytomegalovirus (HCMV) harmfully impacts survival after peripheral blood hematopoietic stem cell transplantation (PB-HSCT). Delayed immune reconstitution after cord blood (CB)-HSCT leads to ...even higher HCMV-related morbidity and mortality. Towards a feasible dendritic cell therapy to accelerate de novo immunity against HCMV, we validated a tricistronic integrase-defective lentiviral vector (coexpressing GM-CSF, IFN-α, and HCMV pp65 antigen) capable to directly induce self-differentiation of PB and CB monocytes into dendritic cells processing pp65 ("SmyleDCpp65"). In vitro, SmyleDCpp65 resisted HCMV infection, activated CD4(+) and CD8(+) T cells and expanded functional pp65-specific memory cytotoxic T lymphocytes (CTLs). CD34(+) cells obtained from PB and CB were transplanted into irradiated NOD.Rag1(-/-).IL2γc(-/-) mice. Donor-derived SmyleDCpp65 administration after PB-HSCT stimulated peripheral immune effects: lymph node remodeling, expansion of polyclonal effector memory CD8(+) T cells in blood, spleen and bone marrow, and pp65-reactive CTL and IgG responses. SmyleDCpp65 administration after CB-HSCT significantly stimulated thymopoiesis. Expanded frequencies of CD4(+)/CD8(+) T cell precursors containing increased levels of T-cell receptor excision circles in thymus correlated with peripheral expansion of effector memory CTL responses against pp65. The comparative in vivo modeling for PB and CB-HSCT provided dynamic and spatial information regarding human T and B cell reconstitution. In vivo potency supports future clinical development of SmyleDCpp65.
Key Data Elements in Myeloid Leukemia Varghese, Julian; Holz, Christian; Neuhaus, Phillip ...
Studies in health technology and informatics,
01/2016, Letnik:
228
Journal Article
Recenzirano
Data standards consisting of key data elements for clinical routine and trial documentation harmonize documentation within and across different health care institutions making documentation more ...efficient and improving scientific data analysis. This work focusses on the field of myeloid leukemia (ML), where a semantic core of common data elements (CDEs) in routine and trial documentation is established by automatic UMLS-based form analysis of existing documentation models. These CDEs (n = 227) were initially reviewed and commented by leukemia experts before they were systematically surveyed by an international voting process through seven hematologists of four countries. The total agreement score was 86%. 116 elements (51%) of these share an agreement score of 100%. This work generated CDEs with language-independent semantic codes and international clinical expert review to build a first approach towards an international data standard for ML. A first version of the CDE list is implemented in the data standard Operational Data Model and additional other data formats for reuse in different medical information systems.
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