Numerous studies have shown that long noncoding RNAs (LncRNAs) are involved in the development and immune escape of head and neck squamous-cell carcinoma (HNSCC). However, the specific regulatory ...mechanisms by which LINC01123 regulates HNSCC and its correlation with immunity remain unclear. Therefore, this study's primary purpose was to explore the mechanisms by which LINC01123 regulates the immune escape and progression of HNSCC. This study confirmed that LINC01123 is competitively bound to miR-214-3p, and miR-214-3p specifically targets B7-H3. The effects of LINC01123, B7-H3, and miR-214-3p on tumor progression, CD8
T-cell-mediated immune response, and the tumorigenicity of HNSCC in vitro and in vivo were examined through the downregulation or upregulation of LINC01123, B7-H3, and miR-214-3p. Our results indicated that LINC01123 and B7-H3 were highly expressed in HNSCC and are associated with poor prognosis in patients. Notably, overexpression of LINC01123 or B7-H3 or downregulation of miR-214-3p inhibited the function of CD8
T cells and promoted the progression of HNSCC. Therefore, LINC01123 acts as a miR-214-3p sponge to inhibit the activation of CD8
T cells and promote the progression of HNSCC by upregulating B7-H3.
The treatment for severe acute pancreatitis (SAP) with Traditional Chinese medicine (TCM) is a hot topic in China. The purpose of our systematic review and meta-analysis is to evaluate the ...effectiveness and safety of rhubarb gavage combined with external application of miralite in SAP patients, to provide some clues for future studies.
All clinical trials on rhubarb nasal feeding combined with mirabilite external application for treating sever acute pancreatitis were searched. RevMan5.4 software was used for statistical analysis.
A total of 732 related articles were obtained in the initial screening. 13 studies were finally included, including 822 patients. The length of hospital stay in the experimental group was shorter than that in the control group MD=-6.80,95%CI(-8.91,-4.69),P < 0.00001. the abdominal pain relief time in the experimental group was shorter than that in the control group MD=-1.82,95%CI(-2.26,-1.39),P < 0.00001. The first defecation time in the experimental group was shorter than that in the control group MD=-1.65,95%CI(-2.06,-1.24),P < 0.00001. The mortality of the experimental group was lower than that of the control group MD=0.45,95%CI(0.22,0.91),P = 0.03.The subgroup with the period less than one week had a shorter length of hospitalization than that out of one week.
Rhubarb nasal feeding combined with mirabilite external application can reduce the time of hospitalization,abdominal pain and distension,and the mortality.Meanwhile, there is no obvious unbeneficial effect. It could be a beneficial and safe treatment approach for SAP.
Background
Perineural invasion (PNI) is a typical pathological characteristic of salivary adenoid cystic carcinoma (SACC) and other neurotrophic cancers. The mechanism of the neural microenvironment ...controlling tumor progression during the PNI process is unclear. In the present study, we investigated the role and molecular mechanisms of nerve‐derived neuropeptide galanin (GAL) and its receptor (GALR2) in the regulation of PNI in SACC.
Methods
Immunohistochemistry staining and clinical association studies were performed to analyze the expression of GAL and GALR2 in SACC tissues and their clinical value. Dorsal root ganglion or SH‐SY5Y cells were co‐cultured with SACC cells in vitro to simulate the interactions between the neural microenvironment and tumor cells, and a series of assays including transcriptome sequencing, Western blot, and Transwell were performed to investigate the role and molecular mechanism of GAL and GALR2 in the regulation of SACC cells. Moreover, both the in vitro and in vivo PNI models were established to assess the potential PNI‐specific therapeutic effects by blocking the GAL/GALR2 axis.
Results
GAL and GALR2 were highly expressed in SACC tissues, and were associated with PNI and poor prognosis in SACC patients (p < 0.05). Nerve‐derived GAL activated GALR2 expression in SACC cells and induced epithelial‐to‐mesenchymal transition (EMT) in SACC cells. Adding human recombinant GAL to the co‐culture system promoted the proliferation, migration, and invasion of SACC cells significantly, but inhibited the apoptosis of SACC cells. Adding M871, a specific antagonist of GALR2, significantly blocked the above effects (p < 0.05) and inhibited the PNI of SACC cells in vitro and in vivo (p < 0.05).
Conclusions
This study demonstrated that nerve‐derived GAL activated GALR2 expression, and promoted EMT in SACC cells, thereby enhancing the PNI process. Interruption of the GAL/GALR2 axis might be a novel strategy for anti‐PNI therapy for SACC.
Nerve‐derived galanin (GAL) could activate GALR2 and promote epithelial‐to‐mesenchymal transition in salivary adenoid cystic carcinoma (SACC) cells, thereby enhancing the perineural invasion (PNI) process. Interruption of the GAL/GALR2 axis might be a novel strategy for anti‐PNI therapy for SACC.
To compare the clinical efficacy of stereotactic body radiotherapy (SBRT) versus surgery for early-stage non-small cell lung cancer through evidence based medicine analysis.
A systematic search was ...performed in the PubMed, EMBASE, CNKI and Wan Fang databases to find studies published before June 2020. Two authors independently extracted the data and assessed the eligibility. All of the statistical analyses were performed using Stata 13.0. Subgroup analysis were performed according to the score matching study and operation type (lobectomy, segmentectomy and thoracoscopic assisted surgery).
Finally, 14 articles were included, including 15,841 cases in SBRT group and 17,708 cases in operation group. 10 articles used propensity score matching methods for survival analysis. Thirteen were retrospective cohort studies and one was randomized controlled trial. The results of meta-analysis showed that the overall survival rate of the surgery group and the SBRT group was statistically significant. The overall survival r
Single-flux-quantum (SFQ) circuits have great potential in building cryogenic quantum-classical interfaces for scaling up superconducting quantum processors. SFQ-based quantum gates have been ...designed and realized. However, current control schemes are difficult to tune the driving strength to qubits, which restricts the gate length and usually induces leakage to unwanted levels. In this study, we design the scheme and corresponding pulse generator circuit to continuously adjust the driving strength by coupling SFQ pulses with variable intervals. This scheme not only provides a way to adjust the SFQ-based gate length, but also proposes the possibility to tune the driving strength envelope. Simulations show that our scheme can suppress leakage to unwanted levels and reduce the error of SFQ-based Clifford gates by more than an order of magnitude.
Empowerment education is a new nursing education model with great significance in the process of chronic disease rehabilitation, and a number of studies have found that it has good benefits for ...patients after percutaneous coronary intervention (PCI). But there is no meta-analysis on how empowerment education influence the life of patients after PCI.
Our study intends to evaluate the impact of empowerment education on the quality of life, cognitive level, anxiety and depression level of patients after PCI.
Systematic review and meta-analysis, following PRISMA guidelines.
RevMan5.4 software and R software were used for statistical analysis. Mean difference or standard mean difference was used as effect analysis statistic for continuous variables with 95% confidence intervals.
Six studies met the inclusion criteria, including 641 patients. The Exercise of Self-Care Agency Scale score of the experimental group was higher than that of the control group, with statistically significant difference. Empowerment education could increase the knowledge of coronary heart disease in patients after PCI, but the difference was not statistically significant.
Significant effects of empowerment have been found in improving patients' quality of life and self-care ability. Empowerment education could be a safe exercise option in PCI rehabilitation. However, the effect of empowerment on cognitive level for coronary heart disease and the depression needs to carry out more large-sample, multi-center clinical trials.
A data-analysis researcher and 3 clinicians are responsible for the writing, and no patients participated in the writing of this paper.
To evaluate the efficacy and safety of Reduning injection for treating community-acquired pneumonia.
Literatures about randomized controlled trials of Reduning injection for treating ...community-acquired pneumonia were reviewed. The methodological quality of the trials was assessed by the Jadad scale, and evaluation was performed.
Seven RCTS were included. Reduning combined group (Reduning injection plus antibiotics and basic therapy) was compared with antibiotics group (antibiotics plus basic therapy). Meta-analysis showed that the relative risk (RR) for the total cure rate was 1.34, and 95% confidence interval (CI) was 1.19, 1.51; RR for the total effective rate was 1.10, and 95% CI was 1.06, 1.15. The weighted mean difference (WMD) in disappearance time of fever between the two groups was - 1.42, and 95% CI was - 2.58, -0.26; The WMD between the two groups for the total obvious effect rate of cough and expectoration were - 2.36, and 95% CI was - 3.41, - 1.31; Improve the time of pulmonary rales MD -2.30, 95% CI - 2.61, - 2.00; The WMD between the two groups in absorption of chest x-ray shadow was -2.36 and 95% CI -2.52, -2.20. Serious systematic adverse reactions had not been reported in the trials.
The effect of combined therapy with Reduning injection plus antibiotics and basic therapy is better than that of antibiotics plus basic therapy. Reduning injection can improve the symptoms of cough and expectoration, shorten the fever time and facilitate the absorption of chest x-ray shadow without any significant adverse reactions. However, further high quality trials are needed.
Suspended particulate matter (SPM) and sediments are important sources of dissolved organic matter (DOM) in lake water. However, studies on what extent and how both sources affect DOM composition are ...lacking, which hampers DOM management. Herein, DOM, SPM-extracted particulate organic matter (POM), and sediment-extracted organic matter (SOM) were characterized and compared in terms of absorption spectral properties and chemical composition in Lake Taihu, a large cyanobacterial bloom-affected shallow lake. A statistical method was proposed to quantify the similarity of organic matter (OM) in the different states and to evaluate the potential effects of SPM and sediments on DOM. Results showed that POM and DOM were mainly composed of small-molecular-size and low-humified organic components (i.e., 27 %–38 % tryptophan-like and ~30 % protein-like substances), and most of them were derived from autochthonous sources. While tyrosine-like (57 %) and humic-like (27 %) substances were dominant in SOM. The OM similarity between POM and DOM was approximately 1.5 times higher than that between SOM and DOM, indicating the greater effect of SPM than sediments on DOM composition. High pH and low nitrogen (e.g., nitrate and ammonia) were positively correlated to the OM similarity between POM and DOM. Further, the findings indicated that nitrogen limitation enhanced the OM exchange between POM and DOM by promoting the production of extracellular polymeric substances (EPS) in cyanobacterial aggregates. The obtained findings highlighted the importance of SPM in shaping the DOM composition relative to sediments and facilitating the DOM management in bloom-affected lakes.
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•DOM, POM, and sediment-extracted OM (SOM) were characterized in Lake Taihu.•The OM exchanges in the multi-pools were evaluated based on a similarity analysis.•POM had a stronger effect (>1.5 times) than SOM on DOM composition.•High pH and low nitrogen conditions enhanced the OM exchange between POM on DOM.
Adsorption is an effective and reliable method to recover phosphorus (P) from sewages. Extracellular polymeric substances (EPS) and agarose are common polysaccharides bioresources, and their ...carboxylic and phenolic hydroxyl groups can directly adsorb P via ion exchange and hydrogen bonding mechanisms. Here, we designed EPS-based and Fe-modified hydrogels (agarose (AG)- and agarose-humic (AH)- based hydrogels) for P recovery. The results showed that AG and AH hydrogels could adsorb 33.9 and 67.7 mg P g−1, respectively. Both as-developed hydrogels showed > 75% of the maximal adsorption capacity in the tested pH conditions (3−10). Coexisting anions (i.e., SO42-, NO3-, and CO32-) did not significantly affect the adsorption performance, except for CO32- which inhibited the process. Intraparticle diffusion controlled the adsorption process. After adsorption, large amounts of FePO4 and Fe2(HPO4)3 were generated in the hydrogels, confirming that ligand exchange was the major driving force.
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•EPS-based hydrogels are made from agarose and humic acid.•EPS-based hydrogel appeared to have excellent phosphorus adsorption performance.•Ligand exchange is the major driving force for hydrogels to trap phosphate.
Superconductivity and the normal-state properties of NbN films can be tuned in a wide range, supplying a suitable platform to investigate the systematical evolution of the superconducting ...performances. Herein, we report the upper critical field of NbN films in both the vertical (B⊥ film) and parallel (B∥ film) orientations over a wide temperature range. Eight samples with the superconducting critical temperature Tc ranging from 2.5 K to 9.8 K are studied. It is found that the magnitudes of the upper critical field in the parallel direction (Bc2∥) exceeds the paramagnetic limiting field Bp in the zero-temperature limit, while the perpendicular one (Bc2⊥) is closely approaching Bp. The temperature dependent Bc2⊥ can be described by the extended Werthamer–Helfand–Hohenberg (WHH) model considering the Pauli spin paramagnetism. Meanwhile, the Bc2∥−T data shows the feature of two-dimensional superconductivity in the temperature range near Tc. Our results show that the Pauli paramagnetic effect is greatly enhanced with the decrease of Tc. The evolution of the obtained Maki parameter with other parameters are discussed to reveal the characteristics of the Pauli paramagnetic effect in this system.
•Bc2|| exceeds the Pauli-limiting field Bp, while Bc2${\perp }$ is closely approaching Bp.•Bc2${\perp }$-T curves can be described by WHH model consideringPauli paramagnetic effect.•The Pauli paramagnetic effect is greatly enhanced with the decrease of Tc.
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