In this work we report the realization of the first low-threshold cryogenic detector that uses diamond as absorber for astroparticle physics applications. We tested two 0.175 g CVD diamond samples, ...each instrumented with a W-TES. The sensors showed transitions at about 25 mK. We present the performance of the diamond detectors and we highlight the best performing one, where we obtained an energy threshold as low as 16.8 eV. This promising result lays the foundation for the use of diamond for different fields of applications where low threshold and excellent energy resolution are required, as i.e. light dark matter searches and BSM physics with coherent elastic neutrino nucleus scattering.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The chemical composition of the human immunodeficiency virus type 1 (HIV-1) membrane is critical for fusion and entry into target cells, suggesting that preservation of a functional lipid bilayer ...organization may be required for efficient infection. HIV-1 acquires its envelope from the host cell plasma membrane at sites enriched in raft-type lipids. Furthermore, infectious particles display aminophospholipids on their surface, indicative of dissipation of the inter-leaflet lipid asymmetry metabolically generated at cellular membranes. By combining two-photon excited Laurdan fluorescence imaging and atomic force microscopy, we have obtained unprecedented insights into the phase state of membranes reconstituted from viral lipids (i.e., extracted from infectious HIV-1 particles), established the role played by the different specimens in the mixtures, and characterized the effects of membrane-active virucidal agents on membrane organization. In determining the molecular basis underlying lipid packing and lateral heterogeneity of the HIV-1 membrane, our results may help develop compounds with antiviral activity acting by perturbing the functional organization of the lipid envelope.
RES-NOVA is a new proposed experiment for the investigation of astrophysical neutrino sources with archaeological Pb-based cryogenic detectors. RES-NOVA will exploit Coherent Elastic neutrino-Nucleus ...Scattering (CEνNS) as detection channel, thus it will be equally sensitive to all neutrino flavors produced by Supernovae (SNe). RES-NOVA with only a total active volume of (60 cm)3 and an energy threshold of 1 keV will probe the entire Milky Way Galaxy for (failed) core-collapse SNe with > 3 σ detection significance. The high detector modularity makes RES-NOVA ideal also for reconstructing the main parameters (e.g. average neutrino energy, star binding energy) of SNe occurring in our vicinity, without deterioration of the detector performance caused by the high neutrino interaction rate. For the first time, distances <3 kpc can be surveyed, similarly to the ones where all known past galactic SNe happened. We discuss the RES-NOVA potential, accounting for a realistic setup, considering the detector geometry, modularity and background level in the region of interest. We report on the RES-NOVA background model and on the sensitivity to SN neutrinos as a function of the distance travelled by neutrinos.
Due to the major role of the sun in heating the earth's surface, the atmospheric planetary boundary layer over land is inherently marked by a diurnal cycle. The afternoon transition, the period of ...the day that connects the daytime dry convective boundary layer to the night-time stable boundary layer, still has a number of unanswered scientific questions. This phase of the diurnal cycle is challenging from both modelling and observational perspectives: it is transitory, most of the forcings are small or null and the turbulence regime changes from fully convective, close to homogeneous and isotropic, toward a more heterogeneous and intermittent state. These issues motivated the BLLAST (Boundary-Layer Late Afternoon and Sunset Turbulence) field campaign that was conducted from 14 June to 8 July 2011 in southern France, in an area of complex and heterogeneous terrain. A wide range of instrumented platforms including full-size aircraft, remotely piloted aircraft systems, remote-sensing instruments, radiosoundings, tethered balloons, surface flux stations and various meteorological towers were deployed over different surface types. The boundary layer, from the earth's surface to the free troposphere, was probed during the entire day, with a focus and intense observation periods that were conducted from midday until sunset. The BLLAST field campaign also provided an opportunity to test innovative measurement systems, such as new miniaturized sensors, and a new technique for frequent radiosoundings of the low troposphere. Twelve fair weather days displaying various meteorological conditions were extensively documented during the field experiment. The boundary-layer growth varied from one day to another depending on many contributions including stability, advection, subsidence, the state of the previous day's residual layer, as well as local, meso- or synoptic scale conditions. Ground-based measurements combined with tethered-balloon and airborne observations captured the turbulence decay from the surface throughout the whole boundary layer and documented the evolution of the turbulence characteristic length scales during the transition period. Closely integrated with the field experiment, numerical studies are now underway with a complete hierarchy of models to support the data interpretation and improve the model representations.
Chimeric antigen receptor (CAR) T cell therapy has made significant strides in the treatment of B-cell malignancies, but its application in treating solid tumors still poses significant challenges. ...Particularly, the widespread use of viral vectors to deliver CAR transgenes into T cells comes with limitations, including high costs and regulatory restrictions, which hinder the translation of novel genetic engineering concepts into clinical applications. Non-viral methods, such as transposon/transposase and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems, offer promising alternatives for stable transgene insertion in CAR-T cells. These methods offer the potential to increase accessibility and efficiency in the development and delivery of CAR-T cell therapies. The main challenge in using non-viral methods, however, is their low knock-in efficiency, which leads to low transgene expression levels. In this review, we discuss recent developments in non-viral approaches for CAR-T cell production, the manufacturing requirements for clinical-grade production of non-viral CAR-T cells, and the adjustments needed in quality control for proper characterization of genomic features and evaluation of potential genotoxicity.
•High costs and regulatory hurdles associated with viral vectors for CAR T therapies.•Non-viral gene delivery may overcome limitations of viral vector gene delivery.•Low knock-in efficiency remains a challenge in non-viral CAR-T cell production.•Focus on advancements in non-viral vector design and clinical manufacturing.•Analytical methods to characterize off-target editing and genotoxicity.
The effect of terrain heterogeneities in one-point measurements is a continuous subject of discussion. Here we focus on the order of magnitude of the advection term in the equation of the evolution ...of temperature as generated by documented terrain heterogeneities and we estimate its importance as a term in the surface energy budget (SEB), for which the turbulent fluxes are computed using the eddy-correlation method. The heterogeneities are estimated from satellite and model fields for scales near 1 km or broader, while the smaller scales are estimated through direct measurements with remotely piloted aircraft and thermal cameras and also by high-resolution modelling. The variability of the surface temperature fields is not found to decrease clearly with increasing resolution, and consequently the advection term becomes more important as the scales become finer. The advection term provides non-significant values to the SEB at scales larger than a few kilometres. In contrast, surface heterogeneities at the metre scale yield large values of the advection, which are probably only significant in the first centimetres above the ground. The motions that seem to contribute significantly to the advection term in the SEB equation in our case are roughly those around the hectometre scales.
Diamond operated as a cryogenic calorimeter is an excellent target for direct detection of low-mass dark matter candidates. Following the realization of the first low-threshold cryogenic detector ...that uses diamond as absorber for astroparticle physics applications, we now present the resulting exclusion limits on the elastic spin-independent interaction cross-section of dark matter with diamond. We measured two 0.175 g CVD (Chemical Vapor Deposition) diamond samples, each instrumented with a Transition Edge Sensor made of Tungsten (W-TES). Thanks to the energy threshold of just 16.8 eV of one of the two detectors, we set exclusion limits on the elastic spin-independent interaction of dark matter particles with carbon nuclei down to dark matter masses as low as 0.122 GeV/c
2
. This work shows the scientific potential of cryogenic detectors made from diamond and lays the foundation for the use of this material as target for direct detection dark matter experiments.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Rare-event search experiments located on-surface, such as short-baseline reactor neutrino experiments, are often limited by muon-induced background events. Highly efficient muon vetos are essential ...to reduce the detector background and to reach the sensitivity goals. We demonstrate the feasibility of deploying organic plastic scintillators at sub-Kelvin temperatures. For the NUCLEUS experiment, we developed a cryogenic muon veto equipped with wavelength shifting fibers and a silicon photo multiplier operating inside a dilution refrigerator. The achievable compactness of cryostat-internal integration is a key factor in keeping the muon rate to a minimum while maximizing coverage. The thermal and light output properties of a plastic scintillation detector were examined. We report first data on the thermal conductivity and heat capacity of the polystyrene-based scintillator UPS-923A over a wide range of temperatures extending below one Kelvin. The light output was measured down to 0.8 K and observed to increase by a factor of 1.61 ± 0.05 compared to 300 K. The development of an organic plastic scintillation muon veto operating in sub-Kelvin temperature environments opens new perspectives for rare-event searches with cryogenic detectors at sites lacking substantial overburden.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The experimental detection of the CE
ν
NS allows the investigation of neutrinos and neutrino sources with all-flavor sensitivity. Given its large content in neutrons and stability, Pb is a very ...appealing choice as target element. The presence of the radioisotope
210
Pb (T
1
/
2
∼
22 yrs) makes natural Pb unsuitable for low-background, low-energy event searches. This limitation can be overcome employing Pb of archaeological origin, where several half-lives of
210
Pb have gone by. We present results of a cryogenic measurement of a 15 g PbWO
4
crystal, grown with archaeological Pb (older than
∼
2000 yrs) that achieved a sub-keV nuclear recoil detection threshold. A ton-scale experiment employing such material, with a detection threshold for nuclear recoils of just 1 keV would probe the entire Milky Way for SuperNovae, with equal sensitivity for all neutrino flavors, allowing the study of the core of such exceptional events.
RES-NOVA is a newly proposed experiment for detecting neutrinos from astrophysical sources, mainly Supernovae, using an array of cryogenic detectors made of PbWO
4
crystals produced from ...archaeological Pb. This unconventional material, characterized by intrinsic high radiopurity, enables low-background levels in the region of interest for the neutrino detection via Coherent Elastic neutrino-Nucleus Scattering (CE
ν
NS). This signal lies at the detector energy threshold,
O
(1 keV), and it is expected to be hidden by naturally occurring radioactive contaminants of the crystal absorber. Here, we present the results of a radiopurity assay on a 0.84 kg PbWO
4
crystal produced from archaeological Pb operated as a cryogenic detector. The crystal internal radioactive contaminations are:
232
Th <40
μ
Bq/kg,
238
U <30
μ
Bq/kg,
226
Ra 1.3 mBq/kg and
210
Pb 22.5 mBq/kg. We also present a background projection for the final experiment and possible mitigation strategies for further background suppression. The achieved results demonstrate the feasibility of realizing this new class of detectors.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
PDF
