Cardiotrophin-1 (CT-1), a member of the interleukin (IL)-6 family, is reported to exhibit a plethora of pleiotropic effects in the heart such as cytoprotective, pro-proliferative and pro-fibrotic ...ones. An extensive research has been devoted on proliferative and profibrotic effects of CT-1 on the heart. Thus the present review has been aimed to critically define the cytoprotective effects of CT-1 and the cellular and molecular mechanisms involved in them. Although many effects of CT-1 have been described on the heart, CT-1 has now also been reported to exhibit important protective effects in other organs such as liver, kidney or nervous system. CT-1 produces its effects through a unique receptor system comprising LIF receptor (LIFRβ) and a common signal transducer, the glycoprotein 130 (gp130). The signaling pathway downstream from gp130 is based on at least, three distinct pathways: 1) the janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway, 2) the p42/44 mitogen-activated protein kinase (p42/44 MAPK) pathway, also known as the extracellular receptor kinase-1/2 (ERK1/2) pathway, and 3) the phosphatidylinositol 3-OH kinase (PI3K)/Akt pathway. Since CT-1 easily achieves its cytoprotective effects via a combination of the above three signaling pathways, it becomes quite necessary to determine which pathway(s) is involved in each particular effect of CT-1. In each of its target organs, CT-1 may also display differential mechanisms of cytoprotection, and thus it is relevant to understand how these mechanisms are locally regulated.
INTRODUCTIONThe influence of tobacco on the microbiological spectrum, resistance-sensitivity pattern and evolution in patients with recurrent urinary tract infections (RUTI) is analyzed. Evaluation ...of the effect of polyvalent bacterial vaccine on the prevention of RUTI and smoking status. MATERIAL AND METHODSRetrospective multicenter study of 855 women with RUTI receiving suppressive antibiotic treatment or bacterial vaccine between 2009 and 2013. Group A (GA): Antibiotic (n=495); Subgroups: GA1 non-smoker (n=417), GA2 smoker (n=78). Group B (GB): Vaccine (n=360); Subgroups: GB1 non-smoker (n=263), GB2 smoker (n=97). VARIABLESAge, pre-treatment UTI, disease-free time (DFT), microbial species, sensitivity and resistance. Follow-up at 3, 6 and 12 months with culture and SF-36 questionnaire. RESULTSMean age 56.51 years (18-75), similar between groups (P=.2257). No difference in the number of pretreatment UTIs (P=.1329) or in the distribution of the bacterial spectrum (P=.7471). DFT was higher in subgroups B compared with A. Urine cultures in GA1: E. coli 62.71% with 8.10% resistance (33% quinolones; 33% cotrimoxazole; 33% quinolones + cotrimoxazole); in GA2 E. coli 61.53% with 75% resistance (16.66% quinolones; 33.33% quinolones + cotrimoxazole; 16.66% amoxicillin-clavulanate; 16.66% erythromycin + phosphomycin + clindamycin) (P=.0133). There were no differences between patients of GA treated with cotrimoxazole and nitrofurantoin (P=.8724). Urine cultures in GB1: E. coli 47.36% with 22.22% resistance (5.55% ciprofloxacin; 5.55% cotrimoxazole; 5.55% ciprofloxacin + cotrimoxazole; 5.55% amoxicillin/clavulanic acid). In GB2 E. coli 70.02% with 61.90% resistances (30.76% quinolones; 30.76% cotrimoxazole; 30.76% quinolones + cotrimoxazole; 17.69% amoxicillin-clavulanic acid) (P=.0144). CONCLUSIONSThe development of bacterial resistance is more frequent among women with smoking habits and recurrent urinary infections. This could influence a worse response to preventive treatments, either with antibiotics or vaccines.
INTRODUCTIONThe classical teaching methodology was based on passive transmission-based learning. The model has changed towards an orientation based on student-centred learning. OBJECTIVEThe objective ...of the study has been to evaluate the students' perception when learning about urinary tract infections, and their perspective about the teaching imparted on this pathology in the various subjects that include ITU in their syllabus. METHODSA cross-sectional analytical study of the responses to an anonymous survey entitled: "Methodology on urine infections. Teaching aspects "issued by 228 students at their fifth year of Medical School, from two promotions. They referred to the following subjects: Pharmacy, Pathophisiology, Gynecology and Obstetrics, Infectious diseases, Microbiology, Nephrology, Pediatrics and Urology. RESULTSThe following variables have been analysed: teaching content, teaching basic aspects of the disease, consideration of teaching methodology and improvement suggestions. Descriptive and inferential statistics were used. CONCLUSIONThe study has concluded that teaching urinary tract infection is perceived in specific subjects related to microorganism (Microbiology), the target organ (Infectious diseases, Urology), affected patients (Pediatrics, Gynecology and Obstetrics) rather than transversal subjects such as Pathophysiology or Pharmacy. The teaching methodology has been considered appropriate by more than 50% of the students in five from the 8 subjects that teach the concept of urinary tract infection. The students suggest convenient changes in current teaching methodology in several subjects that impart the urinary tract infection concept.
RESUMEN Objetivos: La revolución de las tecnologías de la información y la comunicación es la base sobre la que se ha desarrollado el avance de las innovaciones tecnológicas sanitarias y la ...investigación biomédica. Estos avances han sido determinantes en la mejora de la salud, ya que han supuesto nuevas formas de cuidados médicos que contribuyen al aumento de la esperanza de vida, la reducción del dolor y la gestión de las enfermedades. Se pretende valorar el nivel de aceptación de las nuevas tecnologías en médicos que traten el dolor a través de unidades específicas. Material y métodos: Se llevó a cabo un estudio sobre el uso de las nuevas tecnologías de la información en médicos que realizan tratamiento del dolor en centros específicos. Los datos se obtuvieron a partir de la elaboración de un cuestionario online que ha sido enviado a través del correo electrónico a los alumnos del Máster en Tratamiento del Dolor de la USAL. Tras la recogida sistemática de la información, hemos realizado los análisis estadísticos abordando la frecuencia de las variables y las relaciones estadísticamente significativas relevantes para nuestro estudio. Posteriormente realizamos un análisis de factores, análisis de clúster y la construcción de nuevas variables compuestas para sintetizar los datos. Tras esta identificación, el análisis de las relaciones estadísticamente significativas nos ha permitido caracterizar a estos individuos. Finalmente, hemos realizado un análisis utilizado una metodología de regresión logística a través del modelo Logit binomial. La herramienta utilizada para la realización de la encuesta online fue Google Sheets, herramienta englobada dentro de Google Docs, que está diseñada para trabajar a través de internet y de la web. Resultados: El 57 % de los profesionales médicos que contestaron a nuestra encuesta son hombres, mientras que el 43 % de las respuestas pertenecen a mujeres, de Unidad del Dolor (39,46 %), Cuidados Paliativos (21,35 %) y en los Centros de Atención Primaria (CAP). Agregando estos datos obtenemos que el 75,94 % de los profesionales trabajan en entidades públicas, el 9,72 % en entidades privadas y el 11,05 % en ambas. El 79,5 % de los profesionales encuestados no ofrecen su teléfono móvil a los pacientes. Más del 83 % de los profesionales encuestados utilizan muy frecuentemente (29,5 %) o frecuentemente (54,2 %) directrices/protocolos médicos en su práctica clínica; más del 55 % de los profesionales encuestados "Nunca utilizan ordenadores o tecnologías de la información y la comunicación para la realización remota de evaluación diagnóstica o prescripción de medidas terapéuticas". No obstante, un 40 % de los encuestados declaran que han realizado telemedicina. Conclusiones: En general, los datos nos muestran una concepción beneficiosa de las aplicaciones de las tecnologías de la información y la comunicación para la práctica clínica en lo relativo a la digitalización y distribución de la información clínica. Además, se constata, dada la experiencia clínica de los profesionales, la necesidad de cambios organizativos asociados a los nuevos sistemas informáticos.
Breast cancer is a major cause of mortality in women. The transcription factor SNAI2 has been implicated in the pathogenesis of several types of cancer, including breast cancer of basal origin. Here ...we show that SNAI2 is also important in the development of breast cancer of luminal origin in MMTV-ErbB2 mice. SNAI2 deficiency leads to longer latency and fewer luminal tumors, both of these being characteristics of pretumoral origin. These effects were associated with reduced proliferation and a decreased ability to generate mammospheres in normal mammary glands. However, the capacity to metastasize was not modified. Under conditions of increased ERBB2 oncogenic activity after pregnancy plus SNAI2 deficiency, both pretumoral defects-latency and tumor load-were compensated. However, the incidence of lung metastases was dramatically reduced. Furthermore, SNAI2 was required for proper postlactational involution of the breast. At 3 days post lactational involution, the mammary glands of Snai2-deficient mice exhibited lower levels of pSTAT3 and higher levels of pAKT1, resulting in decreased apoptosis. Abundant noninvoluted ducts were still present at 30 days post lactation, with a greater number of residual ERBB2+ cells. These results suggest that this defect in involution leads to an increase in the number of susceptible target cells for transformation, to the recovery of the capacity to generate mammospheres and to an increase in the number of tumors. Our work demonstrates the participation of SNAI2 in the pathogenesis of luminal breast cancer, and reveals an unexpected connection between the processes of postlactational involution and breast tumorigenesis in Snai2-null mutant mice.
INTRODUCTIONProstate cancer (PCa) is the second most common male cancer in the world. Its incidence is estimated to grow to 1.7 million new cases and 499,000 new deaths by 2030. Treatment of OCPC can ...affect patients physically and mentally, as well as their close relationships and their job or career, which conditions health-related quality of life (QoL). OBJECTIVEEvaluate the impact on QoL attributable to the treatment for Organ Confined Prostate Cancer (OCPC). MATERIALS AND METHODSProspective multicenter observational study of 406 patients with OCPC treated from January 2015 to June 2018. The sample was divided into four study groups, according to the type of treatment: radical prostatectomy (RP) (GA), external radiotherapy (ERT) (GB), brachytherapy (BT) (GC) and other treatments different from monotherapy with RP, ERT or BT (GD). RESULTSThe age in GC was lower, the mean Prostate Specific Antigen (PSA) of all patients was 8.13 ng/ml, the group with the highest mean PSA was GB with a mean of 10.43 ng/dL, the mean Tumor Stage (TNM) was 3.82, and GD had the lowest post treatment quality of life. CONCLUSIONOCPC treatment affects QoL. Curative monotherapies, specifically RP and BT, have less effect on QoL than external radiotherapy or other therapeutic alternatives. Urinary incontinence and fistulas secondary to OCPC have the highest impact on QOL impairment. The internationally validated SF 36 questionnaire is a useful cross-sectional measure of QOL to compare the impact of OCPC treatment modalities.
Experimental murine model of renal cancer Padilla-Fernández, B; García-Cenador, M B; Rodríguez-Marcos, P ...
Actas urologicas españolas
41, Številka:
7
Journal Article
The objective of this study was to determine the reproducibility in a murine model of renal tumours of various histological strains that could be useful for investigating the response to target ...drugs.
Development and analysis of the "in vivo" model: tumour xenograft of renal cell carcinomas with Balb/c nude athymic mice. Nontumourous human renal tissue was implanted in the interscapular region of 5 mice, chromophobe renal cell carcinoma was implanted in 5 mice (which, after checking its growth, was prepared for implantation in another 10 mice) and Fuhrman grade 2 clear cell renal cell carcinoma (CCRCC) was implanted in 5 mice (which was also subsequently implanted in 10 mice). We monitored the tumour size, onset of metastases and increase in size and number of tumours. When the size had reached a point greater than or equal to locally advanced or metastatic carcinoma, the animals were euthanised for a pathological and immunohistochemical study and a second phase of implantation.
The subcutaneous xenograft of the healthy tissue did not grow. The animals were euthanised at 6 months and no renal tissue was found. The chromophobe renal cell carcinoma cells grew in the initial phase (100%); however, in the second phase, we observed a chronic lymphomonocyte inflammatory reaction and a foreign body reaction. The CCRCC grew at 5-8 months both in the first and second phase (100%), maintaining the tumour type and grade.
The model with athymic Balb/c nude mice is useful for reproducing CCRCC, with the same histological characteristics and aggressiveness as native human tumours, promoting the development of the second experimental phase.
RESUMEN Objetivos: La información proporcionada por las Encuestas de Condiciones de Trabajo y Salud (ECTS) permite la formulación de programas de acción públicos que generen cambios, fijen ...prioridades y evalúen tendencias. Desde 1987 que se realizó la primera ECTS en España, este instrumento se ha ido consolidando como fuente de datos del sistema de las tecnologías de la información y la comunicación. Los resultados muestran la necesidad de establecer el avance de las innovaciones tecnológicas sanitarias y la investigación biomédica. Estos avances serán determinantes en la mejora de la salud, ya que supondrán nuevas formas de cuidados médicos que contribuyen al aumento de la esperanza de vida, la reducción del dolor y la gestión de las enfermedades. El objetivo de este estudio es valorar el nivel de aceptación de las nuevas tecnologías en médicos que traten el dolor a través de unidades específicas. Material y métodos: Se realizó un estudio para comprobar el uso de las TIC en médicos que realizan su labor asistencial y de investigación en Unidades de Dolor. Este estudio consta de una encuesta online, enviada a través de correo electrónico a los alumnos del máster en Tratamiento del Dolor de la Universidad de Salamanca. Tras la recogida sistemática de la información, se realizó el análisis estadístico abordando la frecuencia de las variables y las relaciones estadísticamente significativas relevantes para nuestro estudio. Posteriormente se realizó un análisis utilizando una metodología de regresión logística a través del modelo Logit binomial. Resultados: De los profesionales médicos que contestaron a nuestra encuesta, se obtuvo que aquellos que son varones y trabajan en Unidades del Dolor eran un 39,46 % y en Cuidados Paliativos eran un 21,35 %. Agregando estos datos obtenemos que el 75,94 % de los profesionales trabajan en entidades públicas, el 9,72 % en entidades privadas y el 11,05 % en ambas. Más del 55 % de los profesionales encuestados "Nunca utilizan ordenadores o Tecnologías de la Información y la Comunicación para la realización remota de evaluación diagnóstica o prescripción de medidas terapéuticas". Conclusiones: Los resultados obtenidos nos muestran que los médicos de unidades de dolor consideran un elemento relevante las aplicaciones de las Tecnologías de la Información y la Comunicación para la práctica clínica. Además, se constata, dada la experiencia clínica de los profesionales, la necesidad de cambios organizativos asociados a los nuevos sistemas informáticos.
The influence of tobacco on the microbiological spectrum, resistance-sensitivity pattern and evolution in patients with recurrent urinary tract infections (RUTI) is analyzed. Evaluation of the effect ...of polyvalent bacterial vaccine on the prevention of RUTI and smoking status.
Retrospective multicenter study of 855 women with RUTI receiving suppressive antibiotic treatment or bacterial vaccine between 2009 and 2013. Group A (GA): Antibiotic (n = 495). Subgroups: GA1 non-smoker (n = 417), GA2 smoker (n = 78). Group B (GB): Vaccine (n = 360). Subgroups: GB1 non-smoker (n = 263), GB2 smoker (n = 97). Variables: Age, pre-treatment UTI, disease-free time (DFT), microbial species, sensitivity and resistance. Follow-up at 3, 6 and 12 months with culture and SF-36 questionnaire.
Mean age 56.51 years (18–75), similar between groups (p = 0.2257). No difference in the number of pretreatment UTIs (p = 0.1329) or in the distribution of the bacterial spectrum (p = 0.7471). DFT was higher in subgroups B compared with A. Urine cultures in GA1: E. coli 62.71% with 8.10% resistance (33% quinolones; 33% cotrimoxazole; 33% quinolones + cotrimoxazole); in GA2 E. coli 61.53% with 75% resistance (16.66% quinolones; 33.33% quinolones + cotrimoxazole; 16.66% amoxicillin-clavulanate; 16.66% erythromycin + phosphomycin + clindamycin) (p = 0, 0133). There were no differences between patients of GA treated with cotrimoxazole and nitrofurantoin (p = 0.8724). Urine cultures in GB1: E. coli 47.36% with 22.22% resistance (5.55% ciprofloxacin; 5.55% cotrimoxazole; 5.55% ciprofloxacin + cotrimoxazole; 5.55% amoxicillin / clavulanic acid). In GB2 E.coli 70.02% with 61.90% resistances (30.76% quinolones; 30.76% cotrimoxazole; 30.76% quinolones + cotrimoxazole; 17.69% amoxicillin-clavulanic acid) (p = 0,0144).
The development of bacterial resistance is more frequent among women with smoking habits and recurrent urinary infections. This could influence a worse response to preventive treatments, either with antibiotics or vaccines.
Se analiza la influencia del tabaco en el espectro microbiológico, patrón de resistencia-sensibilidad y evolución en pacientes con infección de orina de repetición (ITUR). Evaluación del efecto de vacuna bacteriana polivalente en la prevención de las ITUR y el estado como fumador.
Estudio retrospectivo multicéntrico de 855 mujeres con ITUR tratadas con pauta antibiótica supresiva o vacuna bacteriana entre 2009 y 2013. Grupo A (GA): Antibiótico (n = 495). Subgrupos: GA1 no fumadora (n = 417), GA2 fumadora (n = 78).Grupo B (GB): Vacuna (n = 360). Subgrupos: GB1 no fumadora (n = 263), GB2 fumadora (n = 97).Variables: Edad, ITU pre-tratamiento, tiempo libre de enfermedad (TLE), especie microbiana, sensibilidad y resistencia. Seguimiento a 3, 6 y 12 meses con cultivo y cuestionario SF-36.
Edad media 56,51años (18–75), similar entre grupos (p = 0,2257). Sin diferencia en número de ITU pre-tratamiento (p = 0,1329) ni en distribución del espectro bacteriano (p = 0,7471). TLE fue superior en los subgrupos B respecto a los correspondientes A.Urocultivos en GA1: E. coli 62,71% con 8,10% resistencia (33% quinolonas; 33% cotrimoxazol; 33% quinolonas+cotrimoxazol); en GA2 E. coli 61,53% con 75% resistencia (16,66% quinolonas; 33,33% quinolonas + cotrimoxazol; 16,66% amoxi-clavulánico; 16,66% eritromicina+fosfomicina+clindamicina) (p = 0,0133). En GA, no hubo diferencias entre pacientes tratadas con cotrimoxazol y nitrofurantoina (p = 0,8724).Urocultivos en GB1: E. coli 47,36% con 22,22% resistencias (5,55% ciprofloxacino; 5,55% cotrimoxazol; 5,55% ciprofloxacino+cotrimoxazol; 5,55% amoxicilina/clavulánico). En GB2 E.Coli 70,02% con 61,90% resistencias (30,76% quinolonas; 30,76% cotrimoxazol; 30,76% quinolonas+cotrimoxazol; 17,69% amoxi-clavulánico) (p = 0,0144).
En mujeres con hábito tabáquico e infecciones urinarias de repetición es más frecuente la aparición bacterias resistentes, lo cual podría influir en una peor respuesta a los tratamientos preventivos, ya sea antibióticos o vacuna.
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