The present paper summarizes new approaches regarding the progress done
to the understanding of the interaction of Trypanosoma
cruzi-cardiomyocytes. Mannose receptors localized at the surface of
...heart muscle cell are involved in binding and uptake of the parasite.
One of the most striking events in the parasite-heart muscle cells
interaction is the disruption of the actin cytoskeleton. We have
investigated the regulation of the actin mRNA during the cytopathology
induced in myocardial cells by the parasite. T. cruzi invasion
increases calcium resting levels in cardiomyocytes. We have previously
shown that Ca2+ ATPase of the sarcoplasmic reticulum (SERCA) is
involved in the invasion of T. cruzi in cardiomyocytes. Treating the
cells with thapsigargin, a drug that binds to all SERCA ATPases and
causes depletion of intracellular calcium stores, we found a 75%
inhibition in the T. cruzi-cardiomyocytes invasion.
Cardiac damages caused by in vivo infection with Trypanosoma cruzi are still not fully clarified. Here we describe for the first time an in vitro model of fibrosis, hypertrophy, and remodeling ...induced by T. cruzi in cardiomyocyte spheroids (cardiac microtissues). In this new 3-dimensional system, cardiac spheroids showed spontaneous contractility, with typical cardiac morphology and production of extracellular matrix components. There were 4-and 6-fold increases, respectively, in the area and the volume of T. cruzi -infected cardiomyocytes and whole micro-tissues, together with a 50% reduction of the cell population. Immunofluorescence showed increased expression of fibronectin, collagen IV, and laminin in the microtissues 144 h after infection. T. cruzi infection induced an increaseinboththecellularareaandtheextracellularmatrixcomponentsincardiacspheroids,whichcontributed to an increase in total microtissue volume, making this a powerful 3-dimensional in vitro model for the study of cardiac-tissue hypertrophy, fibrosis, and remodeling
A positive Trypanosoma cruzi polymerase chain reaction (PCR) is associated with a worse prognosis in patients with chronic Chagas disease (CD).
To study the association of clinical, ...electrocardiographic, and echocardiographic characteristics and biomarker blood levels with positive T. cruzi PCR in chronic CD.
This is a single-centre observational cross-sectional study. Positive T. cruzi PCR association with clinical, electrocardiographic, and echocardiographic characteristics, and biomarker blood levels were studied by logistic regression analysis. p values < 0.05 were considered significant.
Among 333 patients with chronic CD (56.4% men; 62 ± 10 years), T. cruzi PCR was positive in 41.1%. Stepwise multivariate logistic regression showed an independent association between positive T. cruzi PCR and diabetes mellitus {odds ratio (OR) 0.53 95% confidence interval (CI) 0.30-0.93; p = 0.03}, right bundle branch block OR 1.78 (95% CI 1.09-2.89); p = 0.02, and history of trypanocidal treatment OR 0.13 (95% CI 0.04-0.38); p = 0.0002. Among patients with a history of trypanocidal treatment (n = 39), only four (10%) patients had a positive T. cruzi PCR.
Among several studied parameters, only diabetes mellitus, right bundle branch block, and history of trypanocidal treatment showed an independent association with positive T. cruzi PCR. History of trypanocidal treatment was a strong protective factor against a positive T. cruzi PCR.
Arts and Health Promotion Corbin, J. Hope; Sanmartino, Mariana; Hennessy, Emily Alden ...
2021, 2021-03-29
eBook
Odprti dostop
This open access book offers an overview of the beautiful, powerful, and dynamic array of opportunities to promote health through the arts from theoretical, methodological, pedagogical, and critical ...perspectives. This is the first-known text to connect the disparate inter-disciplinary literatures into a coherent volume for health promotion practitioners, researchers, and teachers. It provides a one-stop depository for using the arts as tools for health promotion in many settings and as bridges across communities, cultures, and sectors. The diverse applications of the arts in health promotion transcend the multiple contexts within which health is created, i.e., individual, community, and societal levels, and has a number of potential health, aesthetic, and social outcomes. Topics covered within the chapters include: Exploring the Potential of the Arts to Promote Health and Social Justice Drawing as a Salutogenic Therapy Aid for Grieving Adolescents in Botswana Community Theater for Health Promotion in Japan From Arts to Action: Project SHINE as a Case Study of Engaging Youth in Efforts to Develop Sustainable Water, Sanitation, and Hygiene Strategies in Rural Tanzania and India Movimiento Ventana: An Alternative Proposal to Mental Health in Nicaragua Using Art to Bridge Research and Policy: An Initiative of the United States National Academy of Medicine Arts and Health Promotion is an innovative and engaging resource for a broad audience including practitioners, researchers, university instructors, and artists. It is an important text for undergraduate- and graduate-level courses, particularly in program planning, research methods (especially qualitative methodology), community health, and applied art classes. The book also is useful for professional development among current health promotion practitioners, community nurses, community psychologists, public health professionals, and social workers.
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