Four groups of hysterectomy-derived and colostrum-deprived pigs were intranasally inoculated with an
Actinobacillus pleuropneumoniae biotype 1-serotype 2 strain (producing RTX toxins ApxII and ...ApxIII, 6 pigs), an
A. pleuropneumoniae biotype 1-serotype 10 strain (producing ApxI, 5 pigs), an
A. pleuropneumoniae biotype 2-serotype 2 strain (producing ApxII, 5 pigs) or saline (controls, 7 pigs). All pigs were exposed to
A. pleuropneumoniae biotype 1-serotype 2 endobronchial challenge. After challenge, severe clinical signs were observed in all control pigs, one pig immunized with the
A. pleuropneumoniae biotype 1-serotype 10 strain and two pigs immunized with the
A. pleuropneumoniae biotype 2-serotype 2 strain. These pigs died within 36 h after challenge and 20 to 50% of the lungs were macroscopically affected. In the other pigs, clinical signs were mild or absent and no or only small, focal lung lesions were observed when euthanized at 48 h after challenge. At the time of challenge neutralizing antibodies against ApxI only, ApxII only and both ApxII and III were present in sera of pigs immunized with the
A. pleuropneumoniae biotype 1-serotype 10 strain, the
A. pleuropneumoniae biotype 2-serotype 2 strain and the
A. pleuropneumoniae biotype 1-serotype 2 strain, respectively. These results indicate that immune mechanisms other than Apx neutralizing antibodies were involved in partial cross-protection of pigs immunized against
A. pleuropneumoniae biotype 1-serotype 10 and challenged with the
A. pleuropneumoniae biotype 1-serotype 2.
This enzyme immunoassay (EIA) of human prolactin (hPRL) involves incubation of sample and anti-hPRL antibodies conjugated to horseradish peroxidase (EC 1.11.1.7) in tubes coated with a second ...antibody to hPRL. The test can be performed within 60 min. No reaction of the antibodies with human placental lactogen and human somatotropin is detectable. The presence of detergent allows assay of both serum and plasma. Precision was improved by including polyethylene glycol in the reaction mixture. To optimize analytical recovery, we added protease inhibitor. Assay of the EIA standards shows good correlation with results for World Health Organization reference preparations. The measurable range is 1 to 400 micrograms/L. Intra- and interassay CVs are about 5%. Comparisons with two RIAs and two other EIAs show reasonably good correlations. The components of our EIA are stable for 18 months.
New compounds isolated from the pyrolysis of cellulose Rijsbergen, R. van (State University of Gent, Gent, Belgium); Anteunis, M; Bruyn, A. de ...
Journal of carbohydrate chemistry,
06/1992, Letnik:
11, Številka:
4
Journal Article
Recenzirano
Two so far unknown minor compounds 1,6-anhydro-beta-D-altropyranose and 1,6-anhydro-beta-D-allopyranose were isolated during the classic pyrolysis of cellulose in the presence of copper powder. ...Although for one of them a new intermediate must be proposed, their occurence can be explained from already proven or postulated intermediates
A randomized trial was carried out with 194 patients to compare the effectiveness of sodium warfarin or aspirin with that of placebo in the prevention of venous thromboembolism after surgery for ...fractured hip. Prophylaxis was commenced postoperatively and continued for 21 days or until patient discharge, whichever was earlier. All patients underwent surveillance with iodine 125-fibrinogen leg scanning and impedance plethysmography, with subsequent venography. Venous thromboembolism occurred in 13 patients (20.0%) in the warfarin group, 27 patients (40.9%) in the aspirin group, and 29 patients (46.0%) in the placebo group. Proximal vein thrombosis or pulmonary embolism occurred in 6 patients (9.2%) in the warfarin group, 7 patients (10.6%) in the aspirin group, and 19 patients (30.2%) in the placebo group. The results of this study show that sodium warfarin therapy is safe and effective in preventing thromboembolic complications in patients undergoing surgery for fractured hip, and that aspirin therapy is an equally safe and effective method for preventing proximal vein thrombosis or pulmonary embolism.
Poly(A)‐containing encephalomyocarditis virus RNA functions as an excellent template for cDNA synthesis in vitro with an RNA‐dependent DNA polymerase in the presence of an oligothymidylate primer. ...Under appropriate conditions, discrete transcripts of increasing chain length were obtained, suitable for sequence analysis. A limited cDNA fragment of 36 nucleotides, primer (dT)10 included, was synthesized when dGTP was omitted from the reaction mixture and its primary structure was elucidated using direct DNA‐sequencing methods. The complement corresponds to the 3′ end of encephalomyocarditis RNA. The hexanucleotide (5′‐3′)(A‐A‐U‐A‐A‐A) found in this sequence is also present in all 3′ non‐coding regions of poly(A)‐containing eukaryotic mRNAs studied until now, in nearly identical positions relative to the poly(A) tail. The possible biological significance of this structural homology is discussed.
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