Sulfur is required for growth of all organisms and is present in a wide variety of metabolites having distinctive biological functions. Sulfur is cycled in ecosystems in nature where conversion of ...sulfate to organic sulfur compounds is primarily dependent on sulfate uptake and reduction pathways in photosynthetic organisms and microorganisms. In vascular plant species, transport proteins and enzymes in this pathway are functionally diversified to have distinct biochemical properties in specific cellular and subcellular compartments. Recent findings indicate regulatory processes of sulfate transport and metabolism are tightly connected through several modes of transcriptional and posttranscriptional mechanisms. This review provides up-to-date knowledge in functions and regulations of sulfur assimilation in plants and algae, focusing on sulfate transport systems and metabolic pathways for sulfate reduction and synthesis of downstream metabolites with diverse biological functions.
Minimum energy (as photon) costs are predicted for core reactions of photosynthesis, for photorespiratory metabolism in algae lacking CO₂ concentrating mechanisms (CCMs) and for various types of ...CCMs; in algae, with CCMs; allowance was made for leakage of CO₂ from the internal pool. These predicted values are just compatible with the minimum measured photon costs of photosynthesis in microalgae and macroalgae lacking or expressing CCMs. More energy-expensive photorespiration, for example for organisms using Rubiscos with lower CO₂–O₂ selectivity coefficients, would be less readily accommodated within the lowest measured photon costs of photosynthesis by algae lacking CCMs. The same applies to the cases of CCMs with higher energy costs of active transport of protons or inorganic carbon species, or greater allowance for significant leakage from the accumulated intracellular pool of CO₂. High energetic efficiency can involve a higher concentration of catalyst to achieve a given rate of reaction, adding to the resource costs of growth. There are no obvious mechanistic interpretations of the occurrence of CCMs algae adapted to low light and low temperatures using the rationales adopted for the occurrence of C₄ photosynthesis in terrestrial flowering plants. There is an exception for cyanobacteria with low-selectivity Form IA or IB Rubiscos, and those dinoflagellates with low-selectivity Form II Rubiscos, for which very few natural environments have high enough CO₂:O₂ ratios to allow photosynthesis in the absence of CCMs.
Oxygenic photosynthesis evolved at least 2.4 Ga; all oxygenic organisms use the ribulose bisphosphate carboxylase-oxygenase (Rubisco)—photosynthetic carbon reduction cycle (PCRC) rather than one of ...the five other known pathways of autotrophic CO 2 assimilation. The high CO 2 and (initially) O 2 -free conditions permitted the use of a Rubisco with a high maximum specific reaction rate. As CO 2 decreased and O 2 increased, Rubisco oxygenase activity increased and 2-phosphoglycolate was produced, with the evolution of pathways recycling this inhibitory product to sugar phosphates. Changed atmospheric composition also selected for Rubiscos with higher CO 2 affinity and CO 2 /O 2 selectivity correlated with decreased CO 2 -saturated catalytic capacity and/or for CO 2 -concentrating mechanisms (CCMs). These changes increase the energy, nitrogen, phosphorus, iron, zinc and manganese cost of producing and operating Rubisco—PCRC, while biosphere oxygenation decreased the availability of nitrogen, phosphorus and iron. The majority of algae today have CCMs; the timing of their origins is unclear. If CCMs evolved in a low-CO 2 episode followed by one or more lengthy high-CO 2 episodes, CCM retention could involve a combination of environmental factors known to favour CCM retention in extant organisms that also occur in a warmer high-CO 2 ocean. More investigations, including studies of genetic adaptation, are needed.
The evolution of organisms capable of oxygenic photosynthesis paralleled a long-term reduction in atmospheric CO2 and the increase in O2. Consequently, the competition between O2 and CO2 for the ...active sites of RUBISCO became more and more restrictive to the rate of photosynthesis. In coping with this situation, many algae and some higher plants acquired mechanisms that use energy to increase the CO2 concentrations (CO2 concentrating mechanisms, CCMs) in the proximity of RUBISCO. A number of CCM variants are now found among the different groups of algae. Modulating the CCMs may be crucial in the energetic and nutritional budgets of a cell, and a multitude of environmental factors can exert regulatory effects on the expression of the CCM components. We discuss the diversity of CCMs, their evolutionary origins, and the role of the environment in CCM modulation.
Using in situ data of 2014–2018, the summers Emiliania huxleyi blooms in the Barents Sea were studied. The blooms were recorded in the upper mixed layer in July and August every year, during which ...they spread to cover large areas and were associated with Atlantic waters. The E. huxleyi abundance revealed interannual variability, with the highest values (up to 12 × 106 cells/L) in July 2016. Bloom is characterized by a sharp seasonal thermocline, water surface temperature of about 7.14–11.7 °C, low silicate (0.45 ± 0.08 μM) and nitrogen (0.74 ± 0.16 μM) concentration, high phosphorus concentration (0.09 ± 0.01 μM) and nitrogen to phosphorus ratio significantly below the Redfield ratio. Data confirming the hypothesis of limiting the growth of diatoms by nitrogen concentration are presented. When E. huxleyi bloomed, its biomass exceeded 70% of the total phytoplankton biomass, species diversity was low, and diatoms were practically absent, and dinoflagellates were usually represented by large species.
•In the Barents Sea, Emiliania huxleyi blooms were recorded in the summer every year from 2014 and 2018.•Low silicon and nitrogen concentrations and N:P ratios are the necessary conditions for the bloom.•Co-occurrence of large dinoflagellates but absence of diatoms is typical for these blooms.
The sulfate facilitation hypothesis suggests that changes in ocean sulfate concentration influenced the rise to dominance of phytoplankton species of the red lineage. The mechanistic reasons for this ...phenomenon are not yet understood. We started to address this question by investigating the differences in S utilization by algae of the green and red lineages and in cyanobacteria cultured in the presence of either 5 mmol · L−1 (approximately equivalent to Paleozoic ocean concentrations) or 30 mmol · L−1 (corresponding to post‐Mesozoic/extant concentrations) sulfate. The activities of the main enzymes involved in SO42− assimilation changed in response to changes in growth sulfate concentration. ATP sulfurylase showed different kinetics in the various taxa, with an especially odd behavior for the dinoflagellate. Sulfate availability had a modest effect on cell organic composition. Species‐specific differences in the use of some elements were instead obvious in algae grown in the presence of different sulfate concentrations, overall confirming that algae of the red lineage do better at high sulfate than algae of the green lineage. The increase in sulfate concentration may thus have had an impact on phytoplankton radiation both through changes in their enzymatic machinery and through indirect repercussion on elemental usage.
Carbon dioxide concentrating mechanisms (also known as inorganic carbon concentrating mechanisms; both abbreviated as CCMs) presumably evolved under conditions of low CO
2
availability. However, the ...timing of their origin is unclear since there are no sound estimates from molecular clocks, and even if there were, there are no proxies for the functioning of CCMs. Accordingly, we cannot use previous episodes of high CO
2
(e.g. the Palaeocene–Eocene Thermal Maximum) to indicate how organisms with CCMs responded. Present and predicted environmental change in terms of increased CO
2
and temperature are leading to increased CO
2
and HCO
3
−
and decreased CO
3
2−
and pH in surface seawater, as well as decreasing the depth of the upper mixed layer and increasing the degree of isolation of this layer with respect to nutrient flux from deeper waters. The outcome of these forcing factors is to increase the availability of inorganic carbon, photosynthetic active radiation (PAR) and ultraviolet B radiation (UVB) to aquatic photolithotrophs and to decrease the supply of the nutrients (combined) nitrogen and phosphorus and of any non-aeolian iron. The influence of these variations on CCM expression has been examined to varying degrees as acclimation by extant organisms. Increased PAR increases CCM expression in terms of CO
2
affinity, whilst increased UVB has a range of effects in the organisms examined; little relevant information is available on increased temperature. Decreased combined nitrogen supply generally increases CO
2
affinity, decreased iron availability increases CO
2
affinity, and decreased phosphorus supply has varying effects on the organisms examined. There are few data sets showing interactions amongst the observed changes, and even less information on genetic (adaptation) changes in response to the forcing factors. In freshwaters, changes in phytoplankton species composition may alter with environmental change with consequences for frequency of species with or without CCMs. The information available permits less predictive power as to the effect of the forcing factors on CCM expression than for their overall effects on growth. CCMs are currently not part of models as to how global environmental change has altered, and is likely to further alter, algal and aquatic plant primary productivity.
The relevance of infochemicals in the relationships between organisms is emerging as a fundamental aspect of aquatic ecology. Exchanges of chemical cues are likely to occur not only between organisms ...of different species, but also between conspecific individuals. Especially intriguing is the investigation of chemical communication in microalgae, because of the relevance of these organisms for global primary production and their key role in trophic webs. Intraspecific communication between algae has been investigated mostly in relation to sexuality and mating. The literature also contains information on other types of intraspecific chemical communication that have not always been explicitly tagged as ways to communicate to conspecifics. However, the proposed role of certain compounds as intraspecific infochemicals appears questionable. In this article, we make use of this plethora of information to describe the various instances of intraspecific chemical communication between conspecific microalgae and to identify thecommontraits and ecological significance of intraspecific communication.Wealso discuss the evolutionary implications of intraspecific chemical communication and the mechanisms by which it can be inherited. A special focus is the genetic diversity among conspecific algae, including the possibility that genetic diversity is an absolute requirement for intraspecific chemical communication.
Women with polycystic ovary syndrome (PCOS) are at an elevated risk of endometrial cancer, which may be associated with the continuous proliferative state caused by the interaction between hormones ...and metabolic factors.
To investigate the impact of hormones and metabolic factors in the proliferation and death of endometrium during the proliferative phase.
Cross-sectional study with 11 women with PCOS and eight normal-cycling non-PCOS controls at the Federal University of the State of Rio de Janeiro from February 2011 to June 2019. Clinical, biochemical, and hormonal data were collected to analyze their influence on the expression of biomarkers related to the endometrial tissue breakdown. Hysteroscopy and endometrial biopsies were conducted, and the endometrial samples underwent immunohistochemistry for markers of apoptosis B-cell lymphoma 2 (BCL2), cleaved caspase-3 (CASP3), fas cell surface death receptor (FAS), FAS ligand (FASLG), BCL2 associated X (BAX), marker of proliferation Ki-67 (MKI67), and cell death using terminal deoxynucleotidyl transferase dUTP nick and labeling (TUNEL).
CASP3 and TUNEL expressions were lower in both stroma and endometrium gland of PCOS women than in controls. MKI67 and homeostasis indexes (BCL2/BAX; FASLG/FAS) in the endometrium of the PCOS group were significantly higher. Body mass index (BMI) values were positively correlated with the expression of MKI67 and MKI67/TUNEL ratio in the endometrial stroma compartment. Fasting insulin levels were positively correlated with the expression of BCL2, and DHEA-S levels were negatively correlated with the expression of CASP3 of women with PCOS.
BMI, insulin, and DHEA-S influence the endometrial homeostasis breakdown in PCOS in the endometrium stroma.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
The need to reduce the CO2 footprint of human activities calls for the utilization of new means of production and new sources of products. Microalgae are a very promising source of a large variety of ...products, from fuels to chemicals for multiple industrial applications (e.g., dyes, pharmaceutical products, cosmetics, food and feed, new materials for high tech manufacture), and for processes such as wastewater treatment. Algae, as photosynthetic organisms, use light to energize the synthesis of organic matter and differently from most terrestrial plants, can be cultured on land that is not used for crop production. We describe the main factors contributing to microalgae productivity in artificial cultivation systems and discuss the research areas that still need investigation in order to pave the way to the generation of photosynthetic cell factories. We shall comment on the main caveats of the possible mode of improving photosynthetic efficiency and to optimize the partitioning of fixed C to products of commercial relevance. We address the problem of the selection of the appropriate strain and of the consequences of their diverse physiology and culture conditions for a successful commercial application. Finally, we shall provide state of the art information on cell factories chassis by means of synthetic biology approaches to produce chemicals of interest.
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