The aim of this study was to demonstrate the potential of a wireless pixelated β+-sensitive intracerebral probe (PIXSIC) for in vivo positron emission tomographic (PET) radiopharmacology in awake and ...freely moving rodents. The binding of
Craclopride to D
dopamine receptors was measured in anesthetized and awake rats following injection of the radiotracer. Competitive binding was assessed with a cold raclopride injection 20 minutes later. The device can accurately monitor binding of PET ligands in freely moving rodents with a high spatiotemporal resolution. Reproducible time-activity curves were obtained for pixels throughout the striatum and cerebellum. A significantly lower
Craclopride tracer-specific binding was observed in awake animals. These first results pave the way for PET tracer pharmacokinetics measurements in freely moving rodents.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Memory retrieval can be facilitated by pretest exposure to cues associated with the original training. The present series of experiments was aimed at investigating whether the effectiveness of the ...retrieval cues might be due to their emotional value and thus be associated to a particular pattern of activation of stress systems. Therefore, the effects of exposing rats to different cueing conditions were investigated both on retention performance and on the level of different stress hormones (ACTH, corticosterone and glucose; the latter as an indirect index of adrenergic/sympathetic nervous system activation). Rats trained in a brightness avoidance discrimination task exhibited an enhancement of the retention performance following exposure to the light discriminative stimulus when delivered 1-day after training and not after 21 days, while exposure to contextual cues led to opposite effects on the retention performance, confirming our previous results. Analyses of the level of stress hormones at the time of testing indicated that when the retrieval cues were effective at the behavioral level, cued rats exhibited higher ACTH plasmatic levels than controls, but did not differ in their glucose or corticosterone levels. Further experiments showed that one day after training, both ACTH and corticosterone levels were elevated in light-cued rats if hormone samples were taken 15 min after cueing. These results show that exposure to an effective retrieval cue is accompanied by the activation of the hypothalamus–pituitary–adrenal axis. The possible involvement of the Corticotropin Releasing Factor at the level of the hypothalamus and amygdala (particularly the central nucleus) on the facilitating effect on retention performance following exposure to aversive training-associated cues is discussed. The present results strengthen the notion that emotion can interact with retrieval processes.
Exposure to training-related cues is known to reactivate associated memory and improves subsequent retention performance under various circumstances. The present studies investigated the neural basis ...of retrieval cue effects, by studying in two separate experiments, the involvement of the medial prefrontal cortex and of the dorsal striatum. Rats with lesions to the prelimbic-infralimbic cortex (PL-IL), to the anterior dorsal cingulate (ACd), and to the lateral and medial parts of the dorsal striatum (lDS and mDS) were first trained in a brightness discrimination avoidance task. One day later, rats were tested after being placed in the cueing box with either no training-related cue or with additional exposures to the light discriminative stimulus. None of the lesions affected the acquisition performance. During the retention test, control rats cued with the light in the box exhibited significantly better retention performance than those simply placed in the box, confirming our previous results. While mDS lesions did not modify effects of the retrieval cue, lDS as well as both PL-IL and ACd lesions blocked the facilitative effects of the discriminative stimulus. The present data indicate that ACd, PL-IL and lDS are involved in processes promoted by exposure to training cues, the nature of which are reviewed and discussed. This study in conjunction with previous ones suggests that retrieval cues activate several subcircuits mainly based on an amygdalo–prefrontal–striatum network. Activation of this network results in an improvement of the expression of the associated conditioned response, and may thus be viewed as increasing the efficacy of the retrieval processes.
Retrograde Amnesia: Forgetting Back Riccio, David C.; Millin, Paula M.; Gisquet-Verrier, Pascale
Current directions in psychological science : a journal of the American Psychological Society,
04/2003, Letnik:
12, Številka:
2
Journal Article
Recenzirano
Recently, the field of animal memory research has seen a resurgence of interest in the mechanisms underlying retrograde amnesia (RA) and in the use of RA as a technique for studying memory processes. ...A recent report from a major neuroscience lab, which demonstrated RA for an old reactivated memory, revitalized the debate regarding the widely accepted memory-consolidation theory of RA. Here, we discuss a number of the characteristics of RA and consider the findings that led to the development of the memory-consolidation hypothesis, as well as those suggesting an alternative retrieval-deficit explanation.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, IZUM, KILJ, NMLJ, NUK, OILJ, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Pre-test exposure to training-related cues is known to improve subsequent retention performance. To identify brain regions engaged in processes promoted by retrieval cues, a brain imaging approach ...using the 6-
14Cglucose autoradiographic technique was used. Sprague–Dawley rats trained in a brightness discrimination avoidance task were submitted to different cueing conditions after a 1- or a 21-day training-to-test interval (TTI). Animals were either non-cued, cued with a box, or cued with a box and the light that served as a discriminative stimulus. Effects of the different cueing conditions on retention performance or on metabolic activity in 58 different brain regions were investigated. Rats cued with the light exhibited a subsequent improvement of their retention performance relative to controls, when tested at the 1- but not 21-days TTI, confirming our previous results. At the 1-day retention interval, a comparison between rats cued with the box and rats cued with the box and the light showed that the light cue significantly increased glucose uptake in a neuronal network composed of the lateral, basal, and central nuclei of the amygdala, the anterior and suprachiasmatic hypothalamic nuclei, the nucleus accumbens, the medial septum, and the insular cortex. In contrast, at the 21-day retention interval, both groups demonstrated similar cerebral metabolic activity. The present results indicate that exposure to a light cue increased metabolic activity in the previously mentioned brain structures only when the light acted as an effective retrieval cue, suggesting an involvement of this network in the processes triggered by a retrieval cue. Arguments are provided supporting the notion that the amygdala may play a key role in these processes. Whether the amygdala is a part of a neural network involved in retrieval processes or in neuromodulating systems that favour the efficacy of retrieval processes is also discussed.
To determine the contribution of the hippocampus in the processing of a configural positive patterning discrimination (PPD) task, discrimination between reinforced presentations of a tone plus light ...compound stimulus and nonreinforced presentations of each of its components (TL+/T-,L-) was examined using a conditioned-suppression paradigm. In the first experiment, rats demonstrated a rapid acquisition of the PPD with an appropriate discriminative responding. Rats submitted to posttraining hippocampal lesions (using multiple injections of ibotenic acid) were no longer able to master correctly the previously solved discrimination, demonstrating significant differences in their response rates during the 2 never-reinforced elemental stimulus presentations. In Experiment II, lesioned rats were not able to correctly learn the PPD, demonstrating the same pattern of responding as in Experiment I. These rats were also severely disrupted in a radial maze elimination task. Experiment IIIa indicated that, in a simple conditioning task (T+, L+), normal rats acquired a rapid conditioned suppression for both stimuli, with the tone being slightly more susceptible to conditioning than the light stimulus. In Experiment IIIb, conditioning to the compound tone plus light stimulus led to a clear conditioning to the tone and almost no conditioning to the light, suggesting an overshadowing from the tone to the light. Similar results were obtained in rats with hippocampal lesions. These results strongly suggest that the disruption showed by rats with hippocampal lesions in the PPD task cannot be due to an alteration of the relative salience of the stimulus. The inability of rats with hippocampal lesions to solve correctly the PPD is due to difficulties in eliminating responding to some unimportant events of the situation, reflecting a deficit in selective attention processes rather than in an ability to process configural stimuli. In the discussion, the putative role of the hippocampus in selective attentional processes is more fully discussed.
The effects of ibotenic lesions of the hippocampus on
conditioning to contextual cues during classical fear conditioning
in rats were evaluated by (a) the amount of freezing elicited by
contextual ...cues and (b) the relative avoidance of a shock
compartment. In Experiment 1, lesions to the hippocampus had no
effect on contextual freezing and marginally affected avoidance
after repeated sessions. Experiment 2 showed that lesions to the
hippocampus disrupted avoidance when tested after a single
conditioning session, while leaving unaffected the acquisition of
contextual freezing. Experiment 3 indicated that these lesions
decreased the acquisition of contextual freezing when higher
footshock intensity was used but had no effect on avoidance after
repeated conditioning sessions. These results show that freezing and
avoidance do not quantify context conditioning similarly. They
further indicate that lesions to the hippocampus may disrupt the
expression of these behaviors used as measures of context
conditioning but not the acquisition of context conditioning per
se.
Effects of neurotoxic lesions of the prelimbic-infralimbic cortex (PL-IL) were examined in rats performing 2 conditional tasks. PL-IL-lesioned rats showed normal acquisition of a visuospatial ...conditional discrimination in a Y maze as well as a tone-light conditional discrimination in an operant chamber, indicating that the PL-IL is not necessary for response selection processes. When the working memory load was subsequently increased in the tone-light conditional discrimination, rats with PL-IL lesions showed a delay-dependent disruption of performance. This suggests a role of the PL-IL in some working memory processes. However, the present results, considered along with previous studies, suggest that the PL-IL does not seem to be directly involved in the processes necessary to maintain specific items over a delay period but rather in the planning of forthcoming behavioral responses on the basis of previously acquired information.
Previously, we reported that posttraining paradoxical sleep deprivation (PSD) resulted in an enhancement of the subsequent avoidance performance for rats trained for 15 trials in a Y-maze brightness ...avoidance discrimination task. A series of experiments were conducted to try to further understand the reasons for results which were contrary to those of the bulk of the sleep-learning literature. Experiment 1 investigated the effectiveness of the PSD technique. Rats (N= 4) were sleep recorded while residing on a “swimming pool” apparatus for 24 h. Compared to their baseline values, all animals showed a very large reduction in paradoxical sleep and spent significantly more time awake. Slow-wave sleep was unchanged. In Experiment 2, proactive motor effects were tested. Rats were deprived of PS for 24 h and then tested in a hole board motor activity task. There was a slight effect of PS deprivation on the day following the PSD and no effect when the rats were retested 1 week later. Experiment 3 investigated possible proactive effects of PSD on avoidance performance. Rats exposed to PSD in the 24 h before training in the Y-maze task did not demonstrate any facilitative effect on the subsequent avoidance performance. Experiment 4 investigated the possibility that the PSD facilitative effect could be due to partial training. Rats were given 75 acquisition trials in the brightness discrimination Y-maze avoidance before being subjected to 24 h of PSD. PS-deprived animals showed superior avoidance scores compared to non-PSD controls when retested 24 h later. In Experiment 5, the same strain of rats (N= 11) were sleep recorded after exposure to a partial acquisition in a Y-maze brightness avoidance discrimination task. They were then continuously monitored for 4 consecutive days. The percent PS for the Trained rats was significantly lower than that for the Control animals. This drop in percent PS was not confined to any particular time period in the 24-h day. None of the other sleep parameters reached significance. Analyses of the present results suggest that PSD exerts its facilitative effects on posttraining consolidation processes. We present arguments suggesting that PSD can have effects opposite to those generally reported, in animals demonstrating poor avoidance abilities, in an avoidance task.
