In Argentina, NDM metallo-β-lactamase was first reported in 2013. By now, it has disseminated throughout the country in diverse Gram negative bacteria. Here, we report the case of a paediatric ...patient that underwent a 1-year hospitalisation due to erythrodermic psoriasis in 2014 and received multiple antimicrobial treatments. During his stay, five isolates were obtained from rectal swabs (rs) or blood culture (bc) suspicious of carbapenemase production: a K. quasipneumoniae subsp. quasipneumoniae (rs), Citrobacter freundii (rs), Escherichia coli (bc), Enterobacter cloacae (rs), and a Serratia marcescens (bc). The isolates were studied with broth microdilution, biparental conjugation and plasmid and whole genome sequencing (Illumina). All isolates harboured an 138,998-bp type 1 IncC plasmid that carried blaNDM-1, bleMBL, blaCMY-6, rmtC, aac(6')-Ib, and sul1 resistance genes. Additionally, the blaNDM-plasmids contained ISKpn8 an insertion sequence previously described as associated only to blaKPC. One isolate, a colistin-resistant E. coli, also carried a mcr-1-containing an IncI2 plasmid, which did not harbour additional resistance. The whole genome of K. quasipneumoniae subsp. quasipneumoniae isolate was fully sequenced. This isolate harboured, additionally to blaNDM, three plasmid-mediated quinolone resistance genes: qnrB4, qnrB52 and aac(6')-Ib-cr1. The E. cloacae isolate also harboured qnrA1. These findings alert to the underestimated horizontal dissemination of multidrug-resistant plasmids limiting treatment options with last resort antimicrobials.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Members of the Wiskott-Aldrich syndrome protein (WASP) family control cytoskeletal dynamics by promoting actin filament nucleation with the Arp2/3 complex. The WASP relative WAVE regulates ...lamellipodia formation within a 400-kilodalton, hetero-pentameric WAVE regulatory complex (WRC). The WRC is inactive towards the Arp2/3 complex, but can be stimulated by the Rac GTPase, kinases and phosphatidylinositols. Here we report the 2.3-ångstrom crystal structure of the WRC and complementary mechanistic analyses. The structure shows that the activity-bearing VCA motif of WAVE is sequestered by a combination of intramolecular and intermolecular contacts within the WRC. Rac and kinases appear to destabilize a WRC element that is necessary for VCA sequestration, suggesting the way in which these signals stimulate WRC activity towards the Arp2/3 complex. The spatial proximity of the Rac binding site and the large basic surface of the WRC suggests how the GTPase and phospholipids could cooperatively recruit the complex to membranes.
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Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Abstract
We present the largest and deepest late-time radio and millimeter survey to date of superluminous supernovae (SLSNe) and long-duration gamma-ray bursts (LGRBs) to search for associated ...nonthermal synchrotron emission. Using the Karl G. Jansky Very Large Array (VLA) and the Atacama Large Millimeter/submillimeter Array (ALMA), we observed 43 sources at 6 and 100 GHz on a timescale of ∼ 1–19 yr post-explosion. We do not detect radio/millimeter emission from any of the sources, with the exception of a 6 GHz detection of PTF10hgi, as well as the detection of 6 GHz emission near the location of the SLSN PTF12dam, which we associate with its host galaxy. We use our data to place constraints on central engine emission due to magnetar wind nebulae and off-axis relativistic jets. We also explore nonrelativistic emission from the SN ejecta, and place constraints on obscured star formation in the host galaxies. In addition, we conduct a search for fast radio bursts (FRBs) from some of the sources using VLA phased-array observations; no FRBs are detected to a limit of 16 mJy (7
σ
; 10 ms duration) in about 40 minutes on source per event. A comparison to theoretical models suggests that continued radio monitoring may lead to detections of persistent radio emission on timescales of ≳ a decade.
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•S. maritima rhizosphere bacteria from the polluted Tinto River estuary is analysed.•PGP properties are studied in the absence and the presence of copper.•Four strains with salt and ...heavy metal resistance and PGP properties are selected.•The selected strains are tested in M. sativa seedlings, enhancing root elongation.•The inoculation of S. maritima may be determinant for restoration programs.
Spartina maritima is a native endangered heavy metal rhizoaccumulator cordgrass naturally growing in southwest coasts of Spain, where is used as a biotool to rehabilitate degraded salt marshes. Fifteen bacterial strains were isolated from the rhizosphere of S. maritima growing in the estuary of the Tinto River, one of the most polluted areas in the world. A high proportion of bacteria were resistant towards several heavy metals. They also exhibited multiple plant growth promoting (PGP) properties, in the absence and the presence of Cu. Bacillus methylotrophicus SMT38, Bacillusaryabhattai SMT48, B. aryabhattai SMT50 and Bacilluslicheniformis SMT51 were selected as the best performing strains. In a gnobiotic assay, inoculation of Medicago sativa seeds with the selected isolates induced higher root elongation. The inoculation of S. maritima with these indigenous metal-resistant PGP rhizobacteria could be an efficient method to increase plant adaptation and growth in contaminated estuaries during restoration programs.
Most vascular flowering plants have the capacity to form symbiotic associations with arbuscular mycorrhizal (AM) fungi. The symbiosis develops in the roots where AM fungi colonize the root cortex and ...form arbuscules within the cortical cells. Arbuscules are enveloped in a novel plant membrane and their establishment requires the coordinated cellular activities of both symbiotic partners. The arbuscule-cortical cell interface is the primary functional interface of the symbiosis and is of central importance in nutrient exchange. To determine the molecular events the underlie arbuscule development and function, it is first necessary to identify genes that may play a role in this process. Toward this goal we used the Affymetrix GeneChip Medicago Genome Array to document the M. truncatula transcript profiles associated with AM symbiosis, and then developed laser microdissection (LM) of M. truncatula root cortical cells to enable analyses of gene expression in individual cell types by RT-PCR.
This approach led to the identification of novel M. truncatula and G. intraradices genes expressed in colonized cortical cells and in arbuscules. Within the arbuscule, expression of genes associated with the urea cycle, amino acid biosynthesis and cellular autophagy was detected. Analysis of gene expression in the colonized cortical cell revealed up-regulation of a lysine motif (LysM)-receptor like kinase, members of the GRAS transcription factor family and a symbiosis-specific ammonium transporter that is a likely candidate for mediating ammonium transport in the AM symbiosis.
Transcript profiling using the Affymetrix GeneChip Medicago Genome Array provided new insights into gene expression in M. truncatula roots during AM symbiosis and revealed the existence of several G. intraradices genes on the M. truncatula GeneChip. A laser microdissection protocol that incorporates low-melting temperature Steedman's wax, was developed to enable laser microdissection of M. truncatula root cortical cells. LM coupled with RT-PCR provided spatial gene expression information for both symbionts and expanded current information available for gene expression in cortical cells containing arbuscules.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Most plants form mutualistic associations with arbuscular mycorrhizal (AM) fungi that are ubiquitous in soils. Through this symbiosis, plants can withstand abiotic and biotic stresses. The underlying ...molecular mechanisms involved in mediating mycorrhiza-induced resistance against insects needs further research, and this is particularly true for potato (Solanum tuberosum L. (Solanales: Solanaceae)), which is the fourth most important crop worldwide. In this study, the tripartite interaction between potato, the AM fungus Rhizophagus irregularis (Glomerales: Glomeraceae), and cabbage looper (Trichoplusia ni Hübner) (Lepidoptera: Noctuidae) was examined to determine whether potato exhibits mycorrhiza-induced resistance against this insect. Plant growth, insect fitness, AM fungal colonization of roots, and transcript levels of defense-related genes were measured in shoots and roots after 5 and 8 d of herbivory on mycorrhizal and nonmycorrhizal plants. AM fungal colonization of roots did not have an effect on potato growth, but root colonization levels increased by herbivory. Larval weight gain was reduced after 8 d of feeding on mycorrhizal plants compared with nonmycorrhizal plants. Systemic upregulation of Allene Oxide Synthase 1 (AOS1), 12-Oxo-Phytodienoate Reductase 3 (OPR3) (jasmonic acid pathway), Protease Inhibitor Type I (PI-I) (anti-herbivore defense), and Phenylalanine Ammonia Lyase (PAL) transcripts (phenylpropanoid pathway) was found during the tripartite interaction. Together, these findings suggest that potato may exhibit mycorrhiza-induced resistance to cabbage looper by priming anti-herbivore defenses aboveground. This study illustrates how mycorrhizal potato responds to herbivory by a generalist-chewing insect and serves as the basis for future studies involving tripartite interactions with other pests.
We present Spitzer Space Telescope 3.6 and 4.5 m observations of the binary neutron star merger GW170817 at 43, 74, and 264 days post-merger. Using the final observation as a template, we uncover a ...source at the position of GW170817 at 4.5 m with a brightness of 22.9 0.3 AB mag at 43 days and 23.8 0.3 AB mag at 74 days (the uncertainty is dominated by systematics from the image subtraction); no obvious source is detected at 3.6 m to a 3 limit of >23.3 AB mag in both epochs. The measured brightness is dimmer by a factor of about 2-3 times compared to our previously published kilonova model, which is based on UV, optical, and near-infrared data at 30 days. However, the observed fading rate and color (m3.6-m4.5 0 AB mag) are consistent with our model. We suggest that the discrepancy is likely due to a transition to the nebular phase, or a reduced thermalization efficiency at such late time. Using the Spitzer data as a guide, we briefly discuss the prospects of observing future binary neutron star mergers with Spitzer (in the Laser Interferometer Gravitational-Wave Observatory (LIGO)/Virgo Observing Run 3) and the James Webb Space Telescope (in LIGO/Virgo Observing Run 4 and beyond).
The full-field solution (FFS) of the seismic radiation from a blasthole is refined with the use of a source function consistent with the shock physics on the detonation shock/rock interface. This ...source function accounts for the directionality of the flow in the detonation front, that is curved under the influence of the confining rock, inducing on it an oblique shock mostly on axial direction, contrary to current models that consider a radial source. The interaction of the explosive/confiner system is solved with the oblique shock local polar theory. From this analysis, pressure (p) versus deflection angle (Θ) polar curves are constructed and plotted in the p-Θ plane. The intersection of the explosive and confiner polar curves corresponds to the possible shock matching solution across the explosive/rock interface, which leads to a shock incident angle. A code has been written in MATLAB to perform the shock polar analysis. The resulting detonation front angle of the explosive with the confiner is considered to build a combined radial/axial source. For this, the displacement potentials for the axial component of the source are formulated and incorporated to the solution. Experimental data from quarry blasting are used to illustrate the application of the FFS model and the calibration of results in terms of attenuation coefficients. The effect of introducing the radial/axial combined source is also discussed.
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