Unlike conventional unsupervised classification methods, such as K-means and ISODATA, which are based on partitional clustering techniques, the methodology proposed in this work attempts to take ...advantage of the properties of Kohonen's self-organizing map (SOM) together with agglomerative hierarchical clustering methods to perform the automatic classification of remotely sensed images. The key point of the proposed method is to execute the cluster analysis process by means of a set of SOM prototypes, instead of working directly with the original patterns of the image. This strategy significantly reduces the complexity of the data analysis, making it possible to use techniques that have not normally been considered viable in the processing of remotely sensed images, such as hierarchical clustering methods and cluster validation indices. Through the use of the SOM, the proposed method maps the original patterns of the image to a two-dimensional neural grid, attempting to preserve the probability distribution and topology of the input space. Afterwards, an agglomerative hierarchical clustering method with restricted connectivity is applied to the trained neural grid, generating a simplified dendrogram for the image data. Utilizing SOM statistic properties, the method employs modified versions of cluster validation indices to automatically determine the ideal number of clusters for the image. The experimental results show examples of the application of the proposed methodology and compare its performance to the K-means algorithm.
Controversies about Mendelian segregation and CAG expansion (CAGexp) instabilities during meiosis in spinocerebellar ataxia type 3/Machado–Joseph disease (SCA3/MJD) need clarification. Additional ...evidence about these issues was obtained from the cohort of all SCA3/MJD individuals living in South Brazil. A survey was carried out to update information registered since 2001. Deaths were checked with the Public Information System, and data was made anonymous. Anticipation and delta‐CAGexp from parent–offspring pairs, and delta‐CAGexp between siblings were obtained. One hundred and fifty‐nine families (94% of the entire registry) were retrieved, comprising 3725 living individuals as of 2015, 625 of these being symptomatic. Minimal prevalence was 6:100,000. Carriers of a CAGexp represented 65.6% of sibs in the genotyped offspring (p < 0.001). Median instability was larger among paternal than maternal transmissions, and instabilities correlated with anticipation (r = 0.38; p = 0.001). Age of the parent correlated to delta‐CAGexp among 115 direct parent–offspring CAGexp transmissions (ρ = 0.23, p = 0.014). In 98 additional kindreds, the delta‐CAGexp between 269 siblings correlated with their delta‐of‐age (ρ = 0.27, p < 0.0001). SCA3/MJD was associated with a segregation distortion favoring the expanded allele in our cohort. Instability of expansion during meiosis was weakly influenced by the age of the transmitting parent at the time of conception.
In this paper, we consider a nonlinear programming problem for which the constraint set may be infeasible. We propose an algorithm based on a large family of augmented Lagrangian functions and ...analyze its global convergence properties taking into account the possible infeasibility of the problem. We show that, in a finite number of iterations, the algorithm stops detecting the infeasibility of the problem or finds an approximate feasible/optimal solution with any required precision. We illustrate, by means of numerical experiments, that our algorithm is reliable for different Lagrangian/penalty functions proposed in the literature.
Eucalyptus grandis (W. Hill ex Maiden) leaf traits and tree growth were studied over 3 years after the establishment of two adjacent complete randomized block designs in southern Brazil. In a ...nitrogen (N) input experiment, a treatment with the application of 120 kg N ha-1 was compared to a control treatment without N addition, and in a potassium (K) input experiment a control treatment without K addition was compared to a treatment with the application of 116 kg K ha-1. Young leaves were tagged 9 months after planting to estimate the effect of N and K fertilizations on leaf lifespan. Leaf mass, specific leaf area and nutrient concentrations were measured on a composite sample per plot every 28 days until the last tagged leaf fell. Successive inventories, destructive sampling of trees and leaf litter fall collection made it possible to assess the effect of N and K fertilization on the dynamics of biomass accumulation in above-ground tree components. Whilst the effects of N fertilization on tree growth only occurred in the first 24 months after planting, K fertilization increased the above-ground net primary production from 4478 to 8737 g m-2 over the first 36 months after planting. The average lifespan of tagged leaves was not modified by N addition but it increased from 111 to 149 days with K fertilization. The peak of leaf production occurred in the second year after planting (about 800 g m-2 year-1) and was not significantly modified (P < 0.05) by N and K fertilizations. By contrast, K addition significantly increased the maximum leaf standing biomass from 292 to 528 g m-2, mainly as a consequence of the increase in leaf lifespan. Potassium fertilization increased the stand biomass mainly through the enhancement in leaf area index (LAI) since growth efficiency (defined as the ratio between woody biomass production and LAI) was not significantly modified. A better understanding of the physiological processes governing the leaf lifespan is necessary to improve process-based models currently used in Eucalyptus plantations.
SETTING: Information about the sputum cells of pulmonary tuberculosis (PTB) patients is scarce. The analysis of sputum cells using optical microscopy (OM) is a well-established method, but it has ...some serious limitations.OBJECTIVE: To establish a new flow cytometry (FC) protocol
for the leucocyte evaluation of sputum samples from PTB patients.DESIGN: A new FC protocol using 0.1% dithiothreitol and 0.5% paraformaldehyde was developed to fluidise sputum samples and kill Mycobacterium tuberculosis, respectively, to allow the analysis of sputum samples collected
from TB patients. The protocol was validated by comparing it with OM, and the cellularity of 30 sputum samples from patients with PTB was evaluated.RESULTS: The comparison between leucocyte subsets analysed using OM and FC showed agreement. Immunophenotyping of leucocytes from sputum samples
showed that neutrophils (95.7%) comprised the largest proportion of sputum cells, followed by monocytes/macrophages (2.6%) and lymphocytes (1.6%). Among the total T-lymphocytes (100%), 12.3% were T-helper cells, 24.1% were cytotoxic T-cells and 62.9% were gamma/delta T; none of the T lymphocytes
had the CD4+/CD8+ phenotype.CONCLUSION: FC is a useful method for evaluating the different subtypes of leucocytes present in the sputum samples of PTB patients.
Aedes aegypti (L.) has become an efficient vector of important arboviruses due to its anthropophilic and domiciliary behaviors. Since the 1980s, dengue affects thousands of people every year in ...Brazil; in Fernando de Noronha (FN), a touristic archipelago, dengue cases have occurred since 2001. Once Ae. aegypti populations are well established in the inhabited areas of FN, the threat of dengue or another arbovirus epidemic is continuously imminent. This study aimed to monitor the DENV serotypes in mosquito samples collected in FN, where at least one resident was clinically diagnosed as dengue patient. Entomological surveillance was conducted in 2011 and 2012. Mosquitoes were sorted by sex and location and were stored in pools. DENV detection was performed using polymerase chain reaction with reverse transcription (RT-PCR) and the Platelia Dengue NS1 Ag. RNA integrity was checked by RT-PCR using rpL8 primers, and the minimum infection rate (MIR) was calculated. In total, 339 pools were analyzed, and only one was positive (DENV-1) by Multiplex RT-PCR (MIR = 1.53). When considering only pools with RNA integrity, the MIR was 2.92. Using the Platelia kit, the MIR was 9.18 (considering all the pools) and 17.54 (only 140 pools with RNA integrity). Our results showed the importance of a constant entomological surveillance in that area, the need to improve storage and transportation protocols, and an endogenous control in the RT-PCR to avoid false-negative results. Finally, our study indicated that the NS1-Ag detection was the most sensitive method and should be used routinely for DENV surveillance in mosquitoes if the serotype identification is not required.
The leaf-cutting ant Atta sexdens Forel (Hymenoptera: Formicidae) is one of the most damaging agricultural pests in the Neotropics. Management strategies predominantly rely on the use of general ...insecticides. What is needed are more species-specific and environmentally friendly options. Parasitioids such as phorid flies (Diptera: Phoridae) may be one such option, but a greater understanding of the ecology of the flies and their ant hosts is essential to devise biological control strategies. Here we report parasitism rates, ant host size, parasitoid abundance per host and resultant sex ratios of two phorid species Apocephalus attophilus Borgmeier and Eibesfeldtphora tonhascai Brown parasitizing A.sexdens. The two species achieved parasitism rates of 1.48 and 1.46%, respectively and the pupal period was 14.7 ± 1.1 days and 22.1 ± 2.8 days, respectively. There was no significant difference between the head capsule width of ants parasitized by either A. attophilus or E. tonhascai. Likewise, there was no significant effect between the head capsule width of parasitized and unparasitized ants for both species. A significant positive correlation was found between the head capsule width of the parasitized ants and the number of adult parasitoids A. attophilus emerged. Ants parasitized by E. tonhascai survived significantly longer than those parasitized by A. attophilus. There was no significant effect of ant head width on the sex ratio of the offspring of either parasitoid species and no significant difference in the sex ratio (male: female) of their offspring. In summary, these data addressed here are important steps when considering natural enemies for biological control. Studying survival of the parasitized ants, parasitoid offspring sex ratio and host size preference allows for a better understanding of ant natural biological control in the field and can help in rearing of A. attophilus and E. tonhascai in laboratory.
Housing nursery pigs according to body weight is an observed common practice in production systems and, supposedly, improves growth performance and reduces body weight (BW) variation. This 42-day ...study evaluated the effects of housing nursery pigs according to BW on performance, onset of feed intake, and aggressive behavior. A total of 504 pigs were ranked by BW at weaning and categorized into three groups of 168 pigs each: light, medium, and heavy. Pigs were randomly distributed to unsorted pens (
Unsorted
) containing 6 pigs of each weight group and sorted pens with 18 pigs from just one group per pen (
Sorted
). From weaning to day 3, pigs were fed a diet containing 1% iron oxide dye and rectal swabs presenting red coloration were evaluated to assess feed intake onset. Eight pens were video recorded to evaluate aggressive behavior. Sorted-Heavy pigs delayed the onset of feed intake (
P
≤ 0.011) and presented more aggressive behaviors than Sorted-Light and Sorted-Medium pigs (
P
≤ 0.036). In
Unsorted
, onset of feed intake showed no differences between weight categories. Also, no differences were observed for aggressive behavior between
Sorted
and
Unsorted
. Final BW showed no differences between
Sorted
and
Unsorted
pigs. The within-pen weight coefficient variation (CV) was slightly different (
P
= 0.042) between
Sorted
and
Unsorted
pigs (13.3 and 15.6%, respectively), at the end of the study. Thus, sorting nursery pigs by BW did not improve growth performance and also, induce a lag of post-weaning feed intake onset and increased fights in Heavy pigs.
Adhesive bonding has become more efficient in the last few decades due to the adhesives developments, granting higher strength and ductility. On the other hand, natural fibre composites have recently ...gained interest due to the low cost and density. It is therefore essential to predict the fracture behavior of joints between these materials, to assess the feasibility of joining or repairing with adhesives. In this work, the tensile fracture toughness (Gnc) of adhesive joints between natural fibre composites is studied, by bonding with a ductile adhesive and co-curing. Conventional methods to obtain Gnc are used for the co-cured specimens, while for the adhesive within the bonded joint, the J-integral is considered. For the J-integral calculation, an optical measurement method is developed for the evaluation of the crack tip opening and adherends rotation at the crack tip during the test, supported by a Matlab sub-routine for the automated extraction of these quantities. As output of this work, an optical method that allows an easier and quicker extraction of the parameters to obtain Gnc than the available methods is proposed (by the J-integral technique), and the fracture behaviour in tension of bonded and co-cured joints in jute-reinforced natural fibre composites is also provided for the subsequent strength prediction. Additionally, for the adhesively-bonded joints, the tensile cohesive law of the adhesive is derived by the direct method.
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