Plants subjected to wounding stress produce secondary metabolites. Several of these metabolites prevent chronic diseases and can be used as colorants, flavors, and as antimicrobials. This ...wound-induced production of plant secondary metabolites is mediated by signaling-molecules such as reactive oxygen species (ROS), ethylene (ET) and jasmonic acid (JA). However, their specific role and interactions that modulate the wound-respond in plants is not fully understood. In the present study, a subtractive cDNA library was generated, to better understand the global response of plants to wounding stress. Carrot (Daucus carota) was used as a model system for this study. A total of 335 unique expressed sequence tags (ESTs) sequences were obtained. ESTs sequences with a putative identity showed involvement in stress-signaling pathways as well as on the primary and secondary metabolism. Inhibitors of ROS biosynthesis, ET action, and JA biosynthesis alone and in combination were applied to wounded-carrots in order to determine, based on relative gene expression data, the regulatory role of ET, JA, and ROS on the wound-response in plants. Our results demonstrate that ROS play a key role as signaling-molecules for the wound-induced activation of the primary and secondary metabolism whereas ET and JA are essential to modulate ROS levels.
Wearable devices are starting to gain popularity, which means that a large portion of the population is starting to acquire these products. This kind of technology comes with a lot of advantages, as ...it simplifies different tasks people do daily. However, as they recollect sensitive data, they are starting to be targets for cybercriminals. The number of attacks on wearable devices forces manufacturers to improve the security of these devices to protect them. Many vulnerabilities have appeared in communication protocols, specifically Bluetooth. We focus on understanding the Bluetooth protocol and what countermeasures have been applied during their updated versions to solve the most common security problems. We have performed a passive attack on six different smartwatches to discover their vulnerabilities during the pairing process. Furthermore, we have developed a proposal of requirements needed for maximum security of wearable devices, as well as the minimum requirements needed to have a secure pairing process between two devices via Bluetooth.
Arid environments are defined by the lack of water availability, which is directly related to the mean annual precipitation (MAP), and high values of solar irradiation, which impacts the community ...composition of animals, plants, and the microbial structure of the soil. Recent advances in NGS technologies have expanded our ability to characterize microbiomes, allowing environmental microbiologists to explore the complete microbial structure. Intending to identify and describe the state-of-the-art of bacterial communities in arid soils at a global scale, and to address the effect that some environmental features may have on them, we performed a systematic review based on the PRISMA guideline. Using a combination of keywords, we identified a collection of 66 studies, including 327 sampled sites, reporting the arid soil bacterial community composition by 16S rDNA gene high-throughput sequencing. To identify factors that can modulate bacterial communities, we extracted the geographical, environmental, and physicochemical data. The results indicate that even though each sampled site was catalogued as arid, they show wide variability in altitude, mean annual temperature (MAT), soil pH and electric conductivity, within and between arid environments. We show that arid soils display a higher abundance of Actinobacteria and lower abundance of Proteobacteria, Cyanobacteria, and Planctomycetes, compared with non-arid soil microbiomes, revealing that microbial structure seems to be strongly modulated by MAP and MAT and not by pH in arid soils. We observed that environmental and physicochemical features were scarcely described among studies, hence, we propose a reporting guideline for further analysis, which will allow deepening the knowledge of the relationship between the microbiome and abiotic factors in arid soil. Finally, to understand the academic collaborations landscape, we developed an analysis of the author's network, corroborating a low degree of connectivity and collaborations in this research topic. Considering that it is crucial to understand how microbial processes develop and change in arid soils, our analysis emphasizes the need to increase collaborations between research groups worldwide.
The role of and needs for extracorporeal membrane oxygenation (ECMO) at a population level during the coronavirus disease (COVID-19) pandemic have not been completely established. Here, a study that ...identifies the cumulative incidence of ECMO use in the first pandemic wave is offered. It has been concluded that documenting nationwide ECMO needs may help in planning ECMO provision for future COVID-19 pandemic waves. The 90-day mortality of the Chilean cohort of ECMO-supported patients with COVID-19 (38.8%) is comparable to that of previous reports.
Habitat management is an important strategy for pest control in integrated pest management (IPM). Various categories of habitat management such as trap cropping, intercropping, natural enemy refuges ...such as ‘beetle banks’, and floral resources for parasitoids and predators, have been used in applied insect ecology for many years. In a broader sense, two mechanisms, the ‘enemies hypothesis' and the ‘resource concentration hypothesis' have been identified as acting independently or combined in pest population dynamics. The ‘enemies hypothesis' directly supports the conservation and enhancement of natural enemies, floral resources such as shelter, nectar, alternative food sources, and pollen (SNAP) to improve conservation biological control. The ‘resource concentration hypothesis’ emphasizes how the host selection behavior of herbivores in a diverse habitat can reduce pest colonization in crops. This review emphasizes the potential of these approaches, as well as possible dis-services, and includes limitations and considerations needed to boost the efficacy of these strategies worldwide.
The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth ...of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejía Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress.
The study of emotional concepts stands at a very interesting intersection between the theoretical debate about the nature of emotions and the debate about the nature of processing concrete concepts ...and abstract concepts. On the one hand, it is debated whether it is possible to differentiate basic emotions from secondary emotions and, on the other hand, whether emotional concepts differ from abstract concepts. In this regard, the prototypical perceptual aspects are considered an important factor both for the differentiation between concrete and abstract concepts and for the differentiation between basic and secondary emotions (facial expressions). Thus, the objective has been to determine if (a) the presence or absence of a prototypical perceptual referent, and (b) the type of concept (referring to emotion and not referring to emotion), produce differences between concepts of basic emotions, secondary emotions and concepts not related to emotions, concrete and abstract, in the tasks of qualification of concreteness, imageability and availability of context and the task of the list of properties, that have been used in previous studies. A total of 86 university students from the suburbs of La Serena - Coquimbo (Chile), all native Spanish speakers, participated in the study. The results show that in the perception of concreteness and in the total of enumerated properties, emotional concepts presented similar results to abstract concepts not related to emotion and there was no difference between basic and secondary emotion concepts. In imageability and context availability, emotional concepts were perceived as different from and more concrete than abstract concepts. In addition, the cause-effect type attributes allowed to clearly differentiate emotional concepts from those not related to emotion and to differentiate between basic and secondary emotion concepts. These types of attributes appear almost exclusively in emotional concepts and are more frequent in basic emotions. These results are partially consistent with the predictions of Neurocultural and Conceptual Act theories about emotions.
Stressors to microbial life include expected physical and chemical concerns such as: temperature, pressure, redox state, pH, ionic strength, osmolarity, UV light exposure and shear forces. The ...diverse genetic diversity of the remarkable number of microbial species on the planet usually means that a species somewhere has evolved the ability to use such compounds as a carbon and/or energy source. Organisms as diverse as bacteria, yeast, and mammals share a requirement for the regulation of cellular content of essential metals to ensure correct function of several metal-binding proteins, like copper, iron, nickel, and zinc. To prevent the consequences of metal overload, living organisms have evolved molecular mechanisms that regulate uptake, intracellular traffic, storage, and efflux, reduction/oxidation to change species, chemical modification, sequestration, repair of damage (typically from ROS) 11,12,13,14.
Efficiently detecting early environmental threats to wildlife is vital for conservation. Beyond obvious dangers like habitat loss or deforestation, our study focuses on one of the most hazardous ...toxic metals for wildlife: lead (Pb). Pb is a widespread, cumulative, and insidious environmental pollutant that can trigger a wide range of physiological, biochemical, and behavioral disorders. In fact, Pb can cause permanent dysfunction of the major stress system, the hypothalamic pituitary adrenal (HPA) axis. We analyzed Pb and cortisol concentrations in fecal samples from Alouatta pigra in southern Mexico. Fecal samples were collected across six sites categorized as free‐ranging (n = 65; conserved and disturbed) and from captive animals (n = 58). Additionally, we collected soil samples (n = 35). We found that Pb was present in 28% of fecal samples and 83% of soil samples. There was a positive relation between fecal and soil Pb levels, and fecal Pb concentration was negatively associated with cortisol levels. However, the claim of Pb being a direct interference with HPA axis requires further investigation. Given our findings, assessing wildlife exposure can be a valuable tool for understanding potential Pb exposure levels in the environment and its possible implications for human health. It can also serve as an early warning system of these consequences.
Highlights
We found the presence of lead (Pb) in soil and fecal samples of captive and free‐ranging black howler monkeys in Southern Mexico.
Feces could be used to qualitatively detect the presence of Pb in organisms and their surroundings, though they do not provide a quantitative measure of Pb levels.
Fecal Pb concentrations were negatively associated with cortisol levels, suggesting that Pb exposure could disrupt endocrine function in nonhuman primates.
The identification of this harmful metal in monkey feces serves as a crucial signal, underscoring the urgency of both conservation efforts and the welfare of local communities.
The origin of tsunamis in the Mediterranean region and its connected seas, including the Marmara Sea, the Black Sea and the SW Iberian Margin in the NE Atlantic Ocean, is reviewed within the ...geological and seismotectonic settings of the region. A variety of historical documentary sources combined with evidence from onshore and offshore geological signatures, geomorphological imprints, observations from selected coastal archeological sites, as well as instrumental records, eyewitnesses accounts and pictorial material, clearly indicate that tsunami sources both seismic and non-seismic (e.g. volcanism, landslides) can be found in all the seas of the region with a variable tsunamigenic potential. Local, regional and basin-wide tsunamis have been documented. An improved map of 22 main tsunamigenic zones and their relative potential for tsunami generation is presented. From west to east, the most important tsunamigenic zones are situated offshore SW Iberia, in the North Algerian margin, in the Tyrrhenian Calabria and Messina Straits, in the western and eastern segments of the Hellenic Arc, in the Corinth Gulf of Central Greece, in the Levantine Sea offshore the Dead Sea Transform Fault and in the eastern side of the Marmara Sea. Important historical examples, including destructive tsunamis associated with large earthquakes, are presented. The mean recurrence of strong tsunamis in the several basins varies greatly but the highest event frequency (1/96years) is observed in the east Mediterranean basin. For most of the historical events it is still unclear which was the causative seismic source and if the tsunami was caused by co-seismic slip, by earthquake-triggered submarine landslides or by a combination of both mechanisms. In pre-historical times, submarine volcanic eruptions (i.e. caldera collapse, massive pyroclastic flows, volcanogenic landslides) and large submarine landslides caused important tsunamis although little is known about their source mechanisms. We conclude that further investigation of the tsunami generation mechanisms is of primary importance in the Mediterranean region. Inputs from tsunami numerical modeling as well as from empirical discrimination criteria for characterizing tsunami sources have been proved particularly effective for recent, well-documented, aseismic landslide tsunamis (e.g., 1963 Corinth Gulf, 1979 Côte d'Azur, 1999 Izmit Bay, 2002 Stromboli volcano). Since the tsunami generation mechanisms are controlled by a variety of factors, and given that the knowledge of past tsunami activity is the cornerstone for undertaking tsunami risk mitigation action, future interdisciplinary research efforts on past tsunamis are needed.
•Tsunamis in the Mediterranean, Marmara Sea, Black Sea and SW Iberia are reviewed.•Historical, geological, archeological and instrumental records are examined.•We evaluate seismic and aseismic generation mechanisms and 22 tsunamigenic zones.•A variety of criteria for tsunami source discrimination are applied.•Strong tsunami repeat time is 90years on average, hazard implications are discussed.
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