Summary
Several observational studies have assessed the association between psoriasis, psoriatic arthritis (PsA) and type 2 diabetes mellitus, with inconclusive results. We set out to investigate the ...association between psoriasis, PsA and type 2 diabetes mellitus. Observational studies assessing the relationship between psoriasis or PsA and type 2 diabetes mellitus up to December 2012 were identified by electronic and hand searches in Medline, Embase, PubMed, the Cochrane Database of Systematic Reviews and Google Scholar. For each study we collected the first author's last name, publication year, country of origin, study design, characteristics of participants (sample size, age and sex), the variables incorporated into the multivariable analyses, and the odds ratios (ORs) of psoriasis associated with diabetes along with the corresponding 95% confidence intervals (CIs). From the data provided in each article, the crude OR was also calculated. Forty‐four observational studies (in 37 articles) were identified for the final analysis. The pooled OR from random‐effects analysis was determined to be 1·76 (95% CI 1·59–1·96). The highest risk was for patients suffering from PsA (OR 2·18, 95% CI 1·36–3·50). We also observed a dose effect in the risk of suffering from type 2 diabetes mellitus, as patients considered as having severe psoriasis had higher risk (OR 2·10, 95% CI 1·73–2·55) than the pooled OR. We perform meta‐regression and sensitivity analyses to explore sources of heterogeneity among the studies and to determine how they would influence the estimates, and found no significant influence in the results of the meta‐analyses. The findings support the association between psoriasis, PsA and type 2 diabetes mellitus. Some caution must be taken in the interpretation of these results because there may be heterogeneity between studies.
What's already known about this topic?
Several observational studies have assessed the association between psoriasis, psoriatic arthritis and type 2 diabetes mellitus, with inconclusive results.
What does this study add?
A systematic review and meta‐analysis including all observational studies up to December 2012.
Investigation of the influence of psoriatic arthritis and the severity of the disease on the risk of diabetes.
Summary
In addition to its well‐documented value in improving the diagnosis of skin tumours, dermoscopy is continually gaining appreciation in the field of general dermatology. Dermoscopy has been ...shown to facilitate the clinical recognition of several inflammatory and infectious diseases, as well as their discrimination from skin tumours. Moreover, recent data indicate that it might also be profitable in assessing the outcome and adverse effects of various treatments. Application of dermoscopy should follow the standard procedure of acquiring information from patient history and clinically evaluating the number, location and morphology of the lesion(s). Four parameters should be assessed when applying dermoscopy in the realm of inflammatory and infectious diseases: (i) morphological vascular patterns; (ii) arrangement of vascular structures; (iii) colours; and (iv) follicular abnormalities, while the presence of other specific features (clues) should also be evaluated. It must be underlined that dermoscopic findings should always be interpreted within the overall clinical context of the patient, integrated with information from the history and the macroscopic examination. With new evidence continuously being gathered, the dermatoscope gradually acquires a role similar to the stethoscope of general practitioners, becoming an irreplaceable clinical tool for dermatologists. In this article, we provide a succinct summary of existing data on dermoscopy in general dermatology. Practical tips are suggested, which can assist clinicians in profitably utilizing and applying the available knowledge in their everyday practice.
What's already known about this topic?
Dermoscopy has well‐documented value in improving the diagnosis of skin tumours.
It is continually gaining appreciation in the field of general dermatology.
What does this study add?
We provide a succinct summary of existing data on dermoscopy in general dermatology.
Practical tips are suggested, which can assist clinicians in profitably utilizing and applying the available knowledge in their everyday practice.
We study the multiphase feedback processes in the central ∼3 kpc of the barred Seyfert 2 galaxy NGC 5643. We used observations of the cold molecular gas (ALMA CO(2−1) transition) and ionized gas ...(MUSE IFU optical emission lines). We studied different regions along the outflow zone, which extends out to ∼2.3 kpc in the same direction (east-west) as the radio jet, as well as nuclear and circumnuclear regions in the host galaxy disk. The CO(2−1) line profiles of regions in the outflow and spiral arms show two or more different velocity components: one associated with the host galaxy rotation, and the others with out- or inflowing material. In the outflow region, the O
III
λ
5007 Å emission lines have two or more components: the narrow component traces rotation of the gas in the disk, and the others are related to the ionized outflow. The deprojected outflowing velocities of the cold molecular gas (median
V
central
∼ 189 km s
−1
) are generally lower than those of the outflowing ionized gas, which reach deprojected velocities of up to 750 km s
−1
close to the active galactic nucleus (AGN), and their spatial profiles follow those of the ionized phase. This suggests that the outflowing molecular gas in the galaxy disk is being entrained by the AGN wind. We derive molecular and ionized outflow masses of ∼5.2 × 10
7
M
⊙
(
α
CO
Galactic
) and 8.5 × 10
4
M
⊙
and molecular and ionized outflow mass rates of ∼51
M
⊙
yr
−1
(
α
CO
Galactic
) and 0.14
M
⊙
yr
−1
, respectively. This means that the molecular phase dominates the outflow mass and outflow mass rate, while the kinetic power and momentum of the outflow are similar in both phases. However, the wind momentum loads (
Ṗ
out
/
Ṗ
AGN
) for the molecular and ionized outflow phases are ∼27−5 (
α
CO
Galactic
and
α
CO
ULIRGs
) and < 1, which suggests that the molecular phase is not momentum conserving, but the ionized phase most certainly is. The molecular gas content (
M
east
∼ 1.5 × 10
7
M
⊙
;
α
CO
Galactic
) of the eastern spiral arm is approximately 50−70% of the content of the western one. We interpret this as destruction or clearing of the molecular gas produced by the AGN wind impacting in the eastern side of the host galaxy (negative feedback process). The increase in molecular phase momentum implies that part of the kinetic energy from the AGN wind is transmitted to the molecular outflow. This suggests that in Seyfert-like AGN such as NGC 5643, the radiative or quasar and the kinetic or radio AGN feedback modes coexist and may shape the host galaxies even at kiloparsec scales through both positive and (mild) negative feedback.
With the advent of flexible electronics, the old fashioned and conventional solid‐state technology will be replaced by conductive inks combined with low‐cost printing techniques. Graphene is an ideal ...candidate to produce conductive inks, due to its excellent conductivity and zero bandgap. The possibility to chemically modify graphene with active molecules opens up the field of responsive conductive inks. Herein, a bioresponsive, electroactive, and inkjet‐printable graphene ink is presented. The ink is based on graphene chemically modified with selected enzymes and an electrochemical mediator, to transduce the products of the enzymatic reaction into an electron flow, proportional to the analyte concentration. A water‐based formulation is engineered to be respectful with the enzymatic activity while matching the stringent requirements of inkjet printing. The efficient electrochemical performance of the ink, as well as a proof‐of‐concept application in biosensing, is demonstrated. The versatility of the system is demonstrated by modifying graphene with various oxidoreductases, obtaining inks with selectivity toward glucose, lactate, methanol, and ethanol.
Herein, the formulation of bioresponsive, electroactive, graphene‐based inks is presented. The inks are based on graphene chemically modified with selected enzymes and an electrochemical mediator, to transduce the products of the enzymatic reaction into an electron flow proportional to the analyte concentration. The produced inks are compatible with inkjet printing, a low‐cost technique that is industrially appealing, automatable, and scalable.
We present the stellar kinematic maps of a large sample of galaxies from the integral-field spectroscopic survey CALIFA. The sample comprises 300 galaxies displaying a wide range of morphologies ...across the Hubble sequence, from ellipticals to late-type spirals. This dataset allows us to homogeneously extract stellar kinematics up to several effective radii. In this paper, we describe the level of completeness of this subset of galaxies withrespect to the full CALIFA sample, as well as the virtues and limitations of the kinematic extraction compared to other well-known integral-field surveys. In addition, we provide averaged integrated velocity dispersion radial profiles for different galaxy types, which are particularly useful to apply aperture corrections for single aperture measurements or poorly resolved stellar kinematics of high-redshift sources. The work presented in this paper sets the basis for the study of more general properties of galaxies that will be explored in subsequent papers of the survey.
The specific characteristics of copy number variations (CNVs) require specific methods of detection and characterization. We developed the Easy One-Step Amplification and Labeling procedure for CNV ...detection (EOSAL-CNV), a new method based on proportional amplification and labeling of amplicons in 1 PCR.
We used tailed primers for specific amplification and a pair of labeling probes (only 1 labeled) for amplification and labeling of all amplicons in just 1 reaction. Products were loaded directly onto a capillary DNA sequencer for fragment sizing and quantification. Data obtained could be analyzed by Microsoft Excel spreadsheet or EOSAL-CNV analysis software. We developed the protocol using the LDLR (low density lipoprotein receptor) gene including 23 samples with 8 different CNVs. After optimizing the protocol, it was used for genes in the following multiplexes: BRCA1 (BRCA1 DNA repair associated), BRCA2 (BRCA2 DNA repair associated), CHEK2 (checkpoint kinase 2), MLH1 (mutL homolog 1) plus MSH6 (mutS homolog 6), MSH2 (mutS homolog 2) plus EPCAM (epithelial cell adhesion molecule) and chromosome 17 (especially the TP53 tumor protein 53 gene). We compared our procedure with multiplex ligation-dependent probe amplification (MLPA).
The simple procedure for CNV detection required 150 min, with <10 min of handwork. After analyzing >240 samples, EOSAL-CNV excluded the presence of CNVs in all controls, and in all cases, results were identical using MLPA and EOSAL-CNV. Analysis of the 17p region in tumor samples showed 100% similarity between fluorescent in situ hybridization and EOSAL-CNV.
EOSAL-CNV allowed reliable, fast, easy detection and characterization of CNVs. It provides an alternative to targeted analysis methods such as MLPA.
Leishmaniasis is a zoonotic disease caused by Leishmania spp., impacts multiple systems and organs. While hematological and biochemical profiles aren’t definitive for diagnosis, recent studies have ...identified the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune-inflammation index (SII) as predictors of morbidity and mortality in critically ill human and dog patients. This study examined 100 dogs diagnosed with leishmaniasis, categorized by the International Renal Interest Society (IRIS) stages 1–4. Additionally, the dogs were divided based on whether they survived less or more than one year (L1Y and G1Y). Control group consisted of 43 dogs. The NLR increased as the disease progressed (IRIS 1–4), presenting statistically significant differences (P<0.05) when compared to the control group (2,37±2,08) IRIS 3 and 4 (4,59±13,39 and 6,99±12,86, respectively), and G1Y and L1Y (3,60±4,02 and 4,87±5,82, respectively). Significant changes in SII were only evident in short-term survivors (L1Y 951,93±1402) and advanced renal disease cases (IRIS 4 stage 1073,68±1901,09). Conversely, PLR remained largely unchanged. In conclusion, these results suggest that the neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII) may serve as potential markers for assessing disease progression and prognosis in dogs diagnosed with leishmaniasis.
•NLR values may be useful for the clinical assessment of the severity of the disease.•SII values increase with the progression of disease, being the first study that relates SII and canine leishmaniasis.•PLR as no statistically significant differences were observed against control.
Background and Purpose
Chronic alcohol consumption alters the gut–brain axis, but little is known about alcohol binge episodes on the functioning of the intestinal barrier. We investigated the ...influence of ethanol binges on bacterial translocation, gut inflammation and immunity, and tight junction (TJ) structure and the ability of the biolipid oleoylethanolamide (OEA) to prevent ethanol binge‐induced intestinal barrier dysfunction.
Experimental Approach
OEA was injected i.p. before repeated ethanol administration by oral gavage. Plasma, spleen, liver and mesenteric lymph nodes (MLN) were collected in sterile conditions for determination of bacterial load. Immune/inflammatory parameters, TJ proteins and apoptotic markers were determined in colonic tissue by RT‐PCR and Western blotting. TJ ultrastructure was examined by transmission electron microscopy.
Key Results
Ethanol binges induced bacterial translocation to the MLN (mainly) and spleen. Colonic tissues showed signs of inflammation, and activation of innate (Toll‐like receptor‐4) and adaptive (IgA) immune systems and TJ proteins (occludin and claudin‐3) were decreased after ethanol binges. Pretreatment with OEA reduced intestinal inflammation and immune activation and partially preserved the TJ structure affected by alcohol binges but had no effect on alcohol‐induced apoptosis. Ultrastructural analyses of colonic TJs revealed dilated TJs in all ethanol groups, with less electron‐dense material in non‐pretreated rats. The protective effects of i.p. OEA did not reduce bacterial translocation to the MLN. However, intragastric OEA administration significantly reduced plasma LPS levels and bacterial translocation to the MLN.
Conclusion and Implications
OEA‐based pharmacotherapies could potentially be useful to treat disorders characterized by intestinal barrier dysfunction, including alcohol abuse.
Mechanochemical treatment with carbohydrates leads to the successful exfoliation of graphite and this can be considered as a green approach to the preparation of graphene. Glucose, fructose and ...saccharose were used and the former showed the best exfoliation behaviour to generate graphene materials with a relatively low number of defects, as evidenced by Raman spectroscopy. The addition of small amounts of water to the ball milling treatment led to the formation of glucose-graphene co-crystals, which exhibited superior properties in terms of colloidal stability and minimization of cell toxicity.
PDF
