Objetivo: extraer y evaluar la actividad antiviral de los compuestos de Chondracanthus chamissoi y Chlorella peruviana contra DENV-2 en células Vero-76. Materiales y métodos: se extrajeron el ...carragenano de Chondracanthus chamissoi, los carbohidratos solubles de Chondracanthus chamissoi y Chlorella peruviana y se realizó la prueba de toxicidad en células VERO-76 y la evaluación de la actividad antiviral. Resultados: se obtuvieron carragenanos de la fase de esporofito y gametofito de Chondracanthus chamissoi, los mismos que fueron identificados, mediante infrarrojo, como k-carragenano. Por cromatografía se identificaron nueve azúcares (ribosa, xilosa, arabinosa, fructuosa, manosa, galactosa, sucrosa, maltosa y lactosa) en la muestra de carbohidratos solubles de Chondracanthus chamissoi fase gametofito y cuatro azucares (glucosa, sucrosa, maltosa y lactosa) en la de Chlorella peruviana. Los compuestos de Chondracanthus chamissoi y la solución de carbohidratos solubles de Chlorella peruviana no presentaron efecto citotóxico; los carbohidratos del extracto crudo de Chlorella peruviana sí los tuvieron. Todas las fracciones del extracto crudo de Chondracanthus chamissoi fase gametofítica fueron positivas por la prueba de reducción del número de placas(50) a la dilución 1:5. El k-carragenano de Chondracanthus chamissoi en ambas fases y los extractos crudos de carbohidratos solubles de Chondracanthus chamissoi, Chlorella peruviana y la solución de carbohidratos solubles de Chlorella peruviana inhibieron el crecimiento del virus dengue, pero no los carbohidratos del extracto crudo de la Chlorella peruviana. Conclusiones: los compuestos obtenidos de Chondracanthus chamissoi y Chlorella peruviana presentan actividad antiviral contra DENV-2 por lo cual es necesario continuar los estudios del potencial antiviral de estos compuestos fraccionados y purificados.
The goal of this study was to identify unique profiles of readers in a sample of 8–16 year olds with higher functioning autism spectrum disorders (HFASD) and examine the profiles in relation to ASD ...symptom severity. Eighty-one students were assessed utilizing a comprehensive reading battery that included basic word reading, language, and comprehension. Using Latent Profile Analysis, four empirically distinct profiles of readers emerged. Next, using the Autism Diagnostic Observation Schedule, Second Edition (Lord et al., Autism diagnostic observation schedule, 2nd edn, Western Psychological Services, Torrance, CA, 2012), analyses were conducted to determine if significant differences existed between profiles as a result of ASD symptomatology. Findings demonstrate the heterogeneous nature of reading profiles in students with HFASD and significant differences between the reading profiles and ASD symptom severity.
Redesigning lignin, the aromatic polymer fortifying plant cell walls, to be more amenable to chemical depolymerization can lower the energy required for industrial processing. We have engineered ...poplar trees to introduce ester linkages into the lignin polymer backbone by augmenting the monomer pool with monolignol ferulate conjugates. Herein, we describe the isolation of a transferase gene capable of forming these conjugates and its xylem-specific introduction into poplar. Enzyme kinetics, in planta expression, lignin structural analysis, and improved cell wall digestibility after mild alkaline pretreatment demonstrate that these trees produce the monolignol ferulate conjugates, export them to the wall, and use them during lignification. Tailoring plants to use such conjugates during cell wall biosynthesis is a promising way to produce plants that are designed for deconstruction.
We aim to understand how human, social, and cultural capitals are associated with the volunteer process, that is, engagement (starting), intensity (number of hours), and cessation (stopping), among ...older adults.
Data from the 2000 through 2008 Health and Retirement Study and the 2001 through 2009 Consumption and Activity Mail Survey provide a sample of 4,526 respondents. Random-effects pooled time series analyses incorporate not only the presence of various types of capital but also the quality of that capital.
Human and cultural capitals were positively associated with increased volunteer involvement. Effects of social capital (relationships in the family, employment status, and the community) depended on the quality of the relationships, not necessarily on their presence alone.
Results suggest that bolstering older adults' capitals, particularly among lower socioeconomic status groups, can increase volunteer engagement and intensity and reduce cessation. Additionally, a variety of organizational policies including respite programs for caregivers and employer policies allowing employees to reduce their work hours might indirectly affect participation rates and commitment. Potential pools of volunteers exist in families, workplaces, and religious organizations, but more research is necessary to identify how to recruit and retain individuals in social networks where volunteer participatory rates are low.
Knowing What It Makes Winterich, Karen Page; Nenkov, Gergana Y.; Gonzales, Gabriel E.
Journal of marketing,
07/2019, Letnik:
83, Številka:
4
Journal Article
Recenzirano
Recycling campaigns abound, but do consumers think about what becomes of those recyclables? This research proposes that product transformation salience (thinking about recyclables turning into new ...products) increases recycling. The authors theorize that consumers are inspired by the transformation of recyclables into new products and that this inspiration motivates them to recycle. The authors demonstrate the effect of product transformation messages on recycling behavior using a recycling campaign (Study 1) and advertisements for products made from recycled plastic (Study 2). Study 3 demonstrates the mediating role of inspiration. Then, three field studies provide robust support for the transformation salience effect through click-through rates for recycling advertisements (Study 4), recycling rates during pre–football game tailgating (Study 5), and a reduction in the amount of recyclable materials incorrectly placed in the landfill bin by students in a university residence hall (Study 6). The authors discuss implications for the design of recycling campaigns and positioning of recycled products in the marketplace as well as theoretical contributions regarding the roles of transformation salience and inspiration in encouraging recycling and other sustainable behaviors.
Abstract
We present the discovery from the TESS mission of two giant planets transiting M-dwarf stars: TOI 4201 b and TOI 5344 b. We also provide precise radial velocity measurements and updated ...system parameters for three other M dwarfs with transiting giant planets: TOI 519, TOI 3629, and TOI 3714. We measure planetary masses of 0.525 ± 0.064
M
J
, 0.243 ± 0.020
M
J
, 0.689 ± 0.030
M
J
, 2.57 ± 0.15
M
J
, and 0.412±0.040
M
J
for TOI 519 b, TOI 3629 b, TOI 3714 b, TOI 4201 b, and TOI 5344 b, respectively. The corresponding stellar masses are 0.372 ± 0.018
M
☉
, 0.635 ± 0.032
M
☉
, 0.522 ± 0.028
M
☉
, 0.626 ± 0.033
M
☉
, and 0.612 ± 0.034
M
☉
. All five hosts have supersolar metallicities, providing further support for recent findings that, like for solar-type stars, close-in giant planets are preferentially found around metal-rich M-dwarf host stars. Finally, we describe a procedure for accounting for systematic errors in stellar evolution models when those models are included directly in fitting a transiting planet system.
Antimicrobial-resistant (AMR) bacteria, such as
species, are an increasingly common cause of hospital-acquired pneumonia, resulting in high mortality and morbidity. Harnessing the host immune ...response to AMR bacterial infection using mesenchymal stem cells (MSCs) is a promising approach to bypass bacterial AMR mechanisms. The administration of single doses of naïve MSCs to ARDS clinical trial patient cohorts has been shown to be safe, although efficacy is unclear. The study tested whether repeated MSC dosing and/or preactivation, would attenuate AMR
-induced established pneumonia. Rat models of established
-induced pneumonia were randomised to receive intravenous naïve or cytomix-preactivated umbilical cord MSCs as a single dose at 24 h post pneumonia induction with or without a subsequent dose at 48 h. Physiological indices, bronchoalveolar lavage (BAL), and tissues were obtained at 72 h post pneumonia induction. A single dose of naïve MSCs was largely ineffective, whereas two doses of MSCs were effective in attenuating
pneumosepsis, improving lung compliance and oxygenation, while reducing bacteria and injury in the lung. Cytomix-preactivated MSCs were superior to naïve MSCs. BAL neutrophil counts and activation were reduced, and apoptosis increased. MSC therapy reduced cytotoxic BAL T cells, and increased CD4
/CD8
ratios. Systemically, granulocytes, classical monocytes, and the CD4
/CD8
ratio were reduced, and nonclassical monocytes were increased. Repeated doses of MSCs-particularly preactivated MSCs-enhance their therapeutic potential in a clinically relevant model of established AMR
-induced pneumosepsis.
Context. The NASA mission TESS is currently doing an all-sky survey from space to detect transiting planets around bright stars. As part of the validation process, the most promising planet ...candidates need to be confirmed and characterized using follow-up observations.
Aims. In this article, our aim is to confirm the planetary nature of the transiting planet candidate TOI-674b using spectroscopic and photometric observations.
Methods. We use TESS, Spitzer, ground-based light curves, and HARPS spectrograph radial velocity measurements to establish the physical properties of the transiting exoplanet candidate TOI-674b. We perform a joint fit of the light curves and radial velocity time series to measure the mass, radius, and orbital parameters of the candidate.
Results. We confirm and characterize TOI-674b, a low-density super-Neptune transiting a nearby M dwarf. The host star (TIC 158588995, V = 14.2 mag, J = 10.3 mag) is characterized by its M2V spectral type with M⋆ = 0.420 ± 0.010 Mꙩ, R⋆ = 0.420 ± 0.013 Rꙩ, and T(eff) = 3514 ± 57 K; it is located at a distance d = 46.16 ± 0.03 pc. Combining the available transit light curves plus radial velocity measurements and jointly fitting a circular orbit model, we find an orbital period of 1.977143 ± 3 × 10^(−6) days, a planetary radius of 5.25 ± 0.17 Rꚛ, and a mass of 23.6 ± 3.3 Mꚛ implying a mean density of ρp =0.91 ± 0.15 g/cu. cm. A non-circular orbit model fit delivers similar planetary mass and radius values within the uncertainties. Given the measured planetary radius and mass, TOI-674b is one of the largest and most massive super-Neptune class planets discovered around an M-type star to date. It is found in the Neptunian desert, and is a promising candidate for atmospheric characterization using the James Webb Space Telescope.
Abstract
Urbanization has spread throughout the globe. Accompanied by rapid population growth, urbanization has resulted in the change of land use/land cover manifested by conversion of forests to ...impervious areas and subsequent alteration of the hydrology in the area. This study sheds light on the impacts of such land cover changes to peak discharges in the Davao River Basin as a result of the watershed alterations. Land cover from 2010 and 2018 were used to represent two (2) levels of urbanization (increasing with year). These were generated from spatial analysis of MODIS imagery data in ArcGIS. This was used in the hydrologic simulation in HEC-HMS to gather the peak discharges for the two events. An increase in peak discharge is observed along with an increase in urbanization. Furthermore, the additional 1.12% of land converted to an impervious area of the basin lead to an increase in peak discharge of 105.85% in the simulations. Moreover, the conversion of areas to impermeable surfaces led to a 52.23% decrease in the infiltration capacity of the sub basins.
Abstract The vulnerability of oligodendrocytes to ischemic injury may contribute to functional loss in diseases of central white matter. Immunocytochemical methods to identify oligodendrocyte injury ...in experimental models rely on epitope availability, and fail to discriminate structural changes in oligodendrocyte morphology. We previously described the use of a lentiviral vector (LV) carrying enhanced green fluorescent protein (eGFP) under the myelin basic protein (MBP) promoter for selective visualization of oligodendrocyte cell bodies and processes. In this study, we used LV-MBP-eGFP to label oligodendrocytes in rat cerebral white matter prior to transient focal cerebral ischemia, and examined oligodendrocyte injury 24 h, 48 h and 1 week post-reperfusion by quantifying cell survival and assaying the integrity of myelin processes. There was progressive loss of GFP+ oligodendrocytes in ischemic white matter at 24 and 48 h. Surviving GFP+ cells had non-pyknotic nuclear morphology and were terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-negative, but there was marked fragmentation of myelin processes as early as 24 h after stroke. One week after stroke, we observed a restoration of GFP+ oligodendrocytes in ischemic white matter, reflected both by cell counts and by structural integrity of myelin processes. Proliferating cells were not the main source of GFP+ oligodendrocytes, as revealed by bromodeoxyuridine (BrdU) incorporation. These observations identify novel transient structural changes in oligodendrocyte cell bodies and myelinating processes, which may have consequences for white matter function after stroke.
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