Solid set canopy delivery systems (SSCDS) are a novel foliar agrochemical delivery system designed as an alternative for airblast sprayers in high density fruit production. This study tested the pest ...management potential, coverage, and chemical deposition of an SSCDS using commercially available microsprinkler components over the course of a growing season. Spray coverage and deposition for a representative airblast sprayer and SSCDS were evaluated using water sensitive paper and tartrazine dye, respectively. Foliar sprays for pest suppression were applied through both systems, and damage assessments were taken at the midpoint and end of the growing season. SSCDS sprays demonstrated similar levels of coverage on the adaxial leaf surface as airblast sprays, but significantly lower coverage on the abaxial surface. However, mean levels of foliar chemical deposition was generally higher in the SSCDS. Evaluations found minimal arthropod and fungal damage in both airblast and SSCDS treated plots compared to untreated trees. The SSCDS was shown to be a viable alternative to the airblast, with inherent advantages such as rapid application time and improved worker safety. Furthermore, higher deposition on SSCDS treated foliage supports the hypothesis that SSCDS provide a higher droplet capture rate in the canopy, with less off-target loss and drift than airblast sprayers.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Abstract
To fully understand microplastics' impact on soil ecosystems, one must recognize soil organisms as not just passively enduring their negative effects, but potentially contributing to ...microplastics' formation, distribution, and dynamics in soil. We investigated the ability of four soil invertebrates, the cricket Acheta domesticus L. (Orthoptera: Gryllidae), the isopod Oniscus asellus L. (Isopoda: Oniscidae), larvae of the beetle Zophobas morio Fabricius (Coleoptera: Tenebrionidae), and the snail Cornu aspersum Müller (Stylommatophora: Helicidae) to fragment macroscopic pieces of weathered or pristine polystyrene (PS) foam. We placed invertebrates into arenas with single PS foam pieces for 24 h, then collected and assessed the microplastic content of each invertebrate's fecal material, its cadaver, and the sand substrate of its arena via hydrogen peroxide digestion, filtration, and fluorescent staining. All taxa excreted PS particles, though snails only to a tiny extent. Beetle larvae produced significantly more microplastics than snails, and crickets and isopods fragmented the weathered PS foam pieces more than the pristine pieces, which they left untouched. A follow-up experiment with pristine PS foam assessed the effect of different treatments mimicking exposure to the elements on fragmentation by isopods. PS foam pieces soaked in a soil suspension were significantly more fragmented than untreated pieces or pieces exposed to UV light alone. These findings indicate that soil invertebrates may represent a source of microplastics to the environment in places polluted with PS foam trash, and that the condition of macroplastic debris likely affects its palatability to these organisms.
Graphical Abstract
A pneumatic spray delivery (PSD)-based solid set canopy delivery system (SSCDS) consists of in-line reservoirs and micro-emitter assemblies distributed throughout perennial crop canopies. The ...existing PSD-based SSCDS uses a large number of reservoirs, i.e., one unit per 3 m of linear spacing, which resulted in high installation and maintenance costs. These reservoirs also produces up to 25% post-spray chemical losses. Therefore, this study aimed to optimize the volumetric capacity and functionality of the existing reservoir for an efficient spray performance and the large-scale commercial adaptation of PSD-based SSCDS. Three reservoirs with volumetric capacities of 370 (1×), 740 (2×), and 1110 mL (3×) were developed to cover a spray span of 3.0, 6.1, and 9.1 m, respectively. Five system configurations with modified reservoirs and spray outlets were evaluated in the laboratory for pressure drop and spray uniformity. The three best system configurations were then field evaluated in a high-density apple orchard. These configurations had reservoirs with 1×, 2×, and 3× volumetric capacity and micro-emitters installed in a three-tier arrangement. Each replicate configuration was installed as a 77 m loop length encompassing 50 apple trees trained in a tall spindle architecture. A pair of water-sensitive paper (WSPs) samplers (25.4 × 25.4 mm) were placed on the abaxial and adaxial leaf surfaces in the bottom, middle, and top third of the canopy to evaluate the spray coverage (%). The PSD-based SSCDS showed no significant difference at the 5% level in terms of coverage among the three reservoir treatments. Coverage was more evenly distributed among the top, middle, and bottom zones for the 2× and 3× as compared to the 1× reservoir treatment. Overall, compared to the 1× reservoirs, the 2× and 3× reservoirs could potentially reduce the system costs by USD 20,000 and USD 23,410 ha−1, respectively, for tall spindle apple orchards and potentially reduce maintenance needs as well.
Several fungal plant pathogens induce 'pseudoflowers' on their hosts to facilitate insect-mediated transmission of gametes and spores. When spores must be transmitted to host flowers to complete the ...fungal life cycle, we predict that pseudoflowers should evolve traits that mimic flowers and attract the most effective vectors in the flower-visiting community. We quantified insect visitation to flowers, healthy leaves and leaves infected with Monilinia vaccinii-corymbosi (Mvc), the causative agent of mummy berry disease of blueberry. We developed a nested PCR assay for detecting Mvc spores on bees, flies and other potential insect vectors. We also collected volatiles from blueberry flowers, healthy leaves and leaves infected with Mvc, and experimentally manipulated specific pathogen-induced volatiles to assess attractiveness to potential vectors. Bees and flies accounted for the majority of contacts with flowers, leaves infected with Mvc and healthy leaves. Flowers were contacted most often, while there was no difference between bee or fly contacts with healthy and infected leaves. While bees contacted flowers more often than flies, flies contacted infected leaves more often than bees. Bees were more likely to have Mvc spores on their bodies than flies, suggesting that bees may be more effective vectors than flies for transmitting Mvc spores to flowers. Leaves infected with Mvc had volatile profiles distinct from healthy leaves but similar to flowers. Two volatiles produced by flowers and infected leaves, cinnamyl alcohol and cinnamic aldehyde, were attractive to bees, while no volatiles manipulated were attractive to flies or any other insects. These results suggest that Mvc infection of leaves induces mimicry of floral volatiles, and that transmission occurs primarily via bees, which had the highest likelihood of carrying Mvc spores and visited flowers most frequently.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The scope of this work rests at the interface between food and energy sustainability. Thermochemical conversion of biomass is an attractive strategy for the production of low-cost biofuels, and ...bio-based insecticides are a more sustainable and often safer alternative for pest management in agricultural production. This work demonstrates a complimentary strategy to access both biofuels and a bio-based insecticide through a catalytic fast pyrolysis process. Technoeconomic modeling shows the bioinsecticidebio-based insecticide can be produced at a cost ≤1.7$ per kg while fully formulated bioinsecticidebio-based insecticides typically sell for ≥6$ per kg, which can significantly reduce the biofuel selling price. Supply chain analysis shows a 46-88% reduction in green-house gas emissions for this agrochemical can be achieved. By using insecticidal activity data from two well-known crop pests, spotted-wing drosophila (
Drosophila suzukii
, Matsumura) and oriental fruit moth (
Grapholita molesta
, Herbst), with an analytical analysis, which achieved ≥99% mass balance closure on the thermochemically derived distillate product, a structure-function relationship between phenol alkylation and insecticidal activity is proposed. An ecotoxicological assessment of the bio-based insecticide was performed using existing data and prediction tools across 18 metrics. It is estimated that a 2000 tonne per day biorefinery can supply 1-5% of the market, which is typical for other moderately scaled chemicals. The mixture of alkylated phenols, used as a bio-based insecticide, is an ideal coproduct that overcomes separation challenges associated with thermochemical streams, such as heterogeneity and reactivity, while providing a more sustainable source for agrochemicals. Synergistic strategies for energy and food production, such as coproduction of bio-based insecticides with biofuels, can be a viable approach to improve sustainability in both sectors.
The efficacy, economics, and sustainability of a bio-based insecticide produced from the catalytic fast pyrolysis of biomass is reported. This synergistic approach to fuels and agrochemical production can improve both energy and food sectors.
Optimally configured solid set canopy delivery systems (SSCDS) can provide adequate spray performance in high-density apple orchards with a minimized risk of off-target pesticide drift. SSCDS ...configured in a shower-down emitter arrangement have been reported to be the simplest and most economical system. However, existing off-the-shelf emitters used in shower-down configurations have resulted in minimal deposition in lower canopy zones. Therefore, this study was focused on the modifications of off-the-shelf emitters to obtain a desirable spray pattern for adequate spray deposition in all the canopy zones. The modifications include redesigning the impact plate of two existing micro-emitters. Field tests were conducted to evaluate the spray performance of SSCDS with the non-modified emitters (treatment: SD1 and SD3) and contrast the results with modified emitters (treatment: SD2 and SD4). While the treatments SD1 and SD3 had off-the-shelf emitters with swivel plate and static spreader, respectively, the treatment SD2 and SD4 had similar emitters with modified impactor plates. In each treatment block, the apple canopy was divided into six zones and sprayed with a 500 ppm fluorescent tracer solution. Mylar cards and water-sensitive paper samplers were placed on the adaxial and abaxial leaf surfaces in each canopy zone to quantify spray deposition and coverage, respectively. The SSCDS treatments retrofitted with modified emitters, i.e., SD2 and SD4, were observed to have uniform and numerically higher deposition and coverage compared to SD1 and SD3. The SSCDS treatment with modified static spreader (i.e., SD4) resulted in the highest overall spray deposition (1405.7 ± 156.4 ng cm−2 mean ± standard error) with improved mid (1121.6 ± 186.9 ng cm−2) and bottom (895.6 ± 149.3 ng cm−2) canopy deposition. Overall, the proposed emitter modification assisted in improved SSCDS spray performances and may be a way forward toward large-scale emplacements of such systems.
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•Black soldier fly (BSF) are not susceptible to entomopathogenic nematodes (EPN).•Wounding 5th instar BSF increases their infection and mortality rate to EPN.•Wounding 5th instar BSF ...increases the production of some species of EPN.•Heterorhabditis bacteriophora does not show a preference for wounded 5th instar BSF.•BSF larvae are a marginal in vivo rearing host for entomopathogenic nematodes.
Production costs and limited regional availability are two key factors limiting the widespread adoption of entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) in biological control programs. We explore the potential of using black soldier fly larvae Hermetia illucens (L.) (Diptera: Stratiomyidae) as an alternative in vivo rearing host to the greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae). We injured black soldier fly larvae to test the hypothesis that the tough cuticle was preventing the penetration of founding infective juveniles (IJs) into the host and egress of offspring from the cadaver. Injuring the black soldier fly larvae increased the infection rate, the number of nematodes entering a host, and the number of IJs harvested from a cadaver. Black soldier fly larvae, however, provided at most 10-fold less IJs compared to G. mellonella. In olfactometer assays, we assessed nematode behavioral responses to wounded black soldier fly larvae. Steinernema carpocapsae did not move towards the insects. Heterorhabditis bacteriophora was attracted to black soldier fly larvae but not G. mellonella. Heterorhabditis bacteriophora did not show a preference for injured black soldier fly larvae over non-injured larvae. Thus, increased colonization on wounded black soldier fly larvae was likely due to additional entry points rather than an increase in their apparency in the soil solution.
Spotted-wing drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), is an invasive pest of softskinned fruits across the globe. Effective monitoring is necessary to manage this pest, but ...suitable attractants are still being identified. In this study, we combined lures with fermenting liquid baits to improve D. suzukii trapping specificity and attractiveness. We also measured the efficiency and specificity of baits/lures during different times of the season; the reproductive status of females among baits/lures; and the effects of locations and crop type on these response variables. We developed a metric that combined mating status and fat content to determine differences in types of females attracted. Lures utilizing yeast and sugar-based volatiles trapped the most D. suzukii. The addition of a commercial lure to yeast and sugar-based lures increased catches in most locations, but was also the least specific to D. suzukii. Apple juice-based chemical lures tended to be most specific to D. suzukii, while lures comprised of a singular attractant tended to trap more D. suzukii with a higher reproductive potential than combinations of attractants. Trap catch and lure specificity was lower during fruit development than fruit ripening. While catch amounts varied by geographic location and crop type, attractants performed similarly relative to each other in each location and crop. Based on the metrics in this study, the yeast and sugar-based attractants were the most effective lures. However, further work is needed to improve early season monitoring, elucidate the effects of physiological status on bait attraction, and understand how abiotic factors influence bait attraction.
Targeting the genome with sequence-specific DNA-binding molecules is a major goal at the interface of chemistry, biology, and precision medicine. Polyamides, composed of N-methylpyrrole and ...N-methylimidazole monomers, are a class of synthetic molecules that can be rationally designed to “read” specific DNA sequences. However, the impact of different chromatin states on polyamide binding in live cells remains an unresolved question that impedes their deployment in vivo. Here, we use cross-linking of small molecules to isolate chromatin coupled to sequencing to map the binding of two bioactive and structurally distinct polyamides to genomes directly within live H1 human embryonic stem cells. This genome-wide view from live cells reveals that polyamide-based synthetic genome readers bind cognate sites that span a range of binding affinities. Polyamides can access cognate sites within repressive heterochromatin. The occupancy patterns suggest that polyamides could be harnessed to target loci within regions of the genome that are inaccessible to other DNA-targeting molecules.
Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae) and entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) are two soil-dwelling biological control agents used to ...manage western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) and fungus gnats Bradysis spp. (Diptera: Sciaridae) in glasshouses. Growers often use multiple natural enemies to achieve economic control, but knowledge of interactions among natural enemies is lacking. We conducted a laboratory bioassay to test the pathogenicity of four commercially available nematode species-Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhbditidae), Steinernema carpocapsae (Weiser) (Rhabditida: Steinernematidae), S. feltiae (Filipjev), and S. riobrave Cabanillas et al.-to third instar and adult D. coriaria. Third instars were three times more susceptible than the adults to the entomopathogenic nematodes. Mortality for D. coriaria adults and third instars treated with S. feltiae and H. bacteriophora was lower than the mortality for D. coriaria adults and third instars treated with S. carpocapsae and S. riobrave. Neither infective juvenile foraging behavior nor size correlates with D. coriaria mortality. Dalotia coriaria appears to be most likely compatible with applications of S. feltiae and H. bacteriophora.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
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