We report new Gaussian boson sampling experiments with pseudo-photon-number-resolving detection, which register up to 255 photon-click events. We consider partial photon distinguishability and ...develop a more complete model for the characterization of the noisy Gaussian boson sampling. In the quantum computational advantage regime, we use Bayesian tests and correlation function analysis to validate the samples against all current classical spoofing mockups. Estimating with the best classical algorithms to date, generating a single ideal sample from the same distribution on the supercomputer Frontier would take ∼600 yr using exact methods, whereas our quantum computer, Jiǔzhāng 3.0, takes only 1.27 μs to produce a sample. Generating the hardest sample from the experiment using an exact algorithm would take Frontier∼3.1×10^{10} yr.
Gaussian boson sampling (GBS) is not only a feasible protocol for demonstrating quantum computational advantage, but also mathematically associated with certain graph-related and quantum chemistry ...problems. In particular, it is proposed that the generated samples from the GBS could be harnessed to enhance the classical stochastic algorithms in searching some graph features. Here, we use Jiǔzhāng, a noisy intermediate-scale quantum computer, to solve graph problems. The samples are generated from a 144-mode fully connected photonic processor, with photon click up to 80 in the quantum computational advantage regime. We investigate the open question of whether the GBS enhancement over the classical stochastic algorithms persists-and how it scales-with an increasing system size on noisy quantum devices in the computationally interesting regime. We experimentally observe the presence of GBS enhancement with a large photon-click number and a robustness of the enhancement under certain noise. Our work is a step toward testing real-world problems using the existing noisy intermediate-scale quantum computers and hopes to stimulate the development of more efficient classical and quantum-inspired algorithms.
Staphylococcus aureus (S. aureus) is a widespread foodborne pathogen, causing a serious threat to human health and public safety. Thus, it is significant for rapid S. aureus determination. Herein, a ...visual and smartphone-integrated aptasensor for intact S. aureus was fabricated based on the Exo III-assisted recycling and hemin/G-quadruplex DNAzymes for signal amplification. Different concentrations of S. aureus caused the difference in releasing different amounts of DNAzymes via Exo III-assisted recycling reaction. These DNAzymes catalyzed the oxidation of 2,2′-azino-bis(3-ethylbenzothiozoline-6-sulfonic acid) (ABTS2-) to colored ABTS•−, which were respectively analyzed by smartphone and UV-Vis spectrophotometer for quantification. This assay exhibited good analytical performance with a detection limit of 32 CFU·mL−1 and a wide linear range. It also possesses the ability in complex sample matrices detection, low cost, and portability with the employment of a smartphone, making it a rapid, simple, and point-of-care test (POCT) method with great potential in food safety control.
•A smartphone-integrated dual-readout aptasensor for Staphylococcus aureus was constructed.•This method showed a good analytical performance and met the POCT requirement for application in food safety control.•A versatile platform for simple and intact detection of biomolecules was developed.
Emodin is a natural anthraquinone derivative that is present in various herbal preparations. The pharmacological effects of emodin include anticancer, hepatoprotective, anti-inflammatory, antioxidant ...and even antimicrobial activities. However, emodin also has been reported to induce hepatotoxicity, nephrotoxicity, genotoxicity and reproductive toxicity. The mechanism of emodin's adverse effects is complicated and currently not well understood. This study aimed to establish a cell metabonomic method to investigate the toxicity of emodin and explore its potential mechanism and relevant targets. In the present study, metabonomic profiles of cell extracts and cell culture media obtained using the
H NMR technique were used to assess emodin toxicity in HepG2 cells. Multivariate statistical analyses such as partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to characterize the metabolites that differed between the control and emodin groups. The results indicated that emodin resulted in differences in 33 metabolites, including acetate, arginine, aspartate, creatine, isoleucine, leucine and histidine in the cell extract samples and 23 metabolites, including alanine, formate, glutamate, succinate and isoleucine, in the cell culture media samples. Approximately 8 pathways associated with these metabolites were disrupted in the emodin groups. These results demonstrated the potential for using cell metabonomics approaches to clarify the toxicological effects of emodin, the underlying mechanisms and potential biomarkers. Our findings may help with the development of novel strategies to discover targets for drug toxicity, elucidate the changes in regulatory signal networks and explore its potential mechanism of action.
With the continuous development of society and the rapid development of science and technology, virtual reality technology is also developing rapidly. It has been widely used in all walks of life and ...is playing an irreplaceable role. Modern education has also begun to integrate with science. Sports are also constantly deepening the application of information technology. In order to make students have a new understanding of college physical education and sports training and improve the technical level and training quality of college sports athletes, this paper studies the application of virtual reality technology in physical education and sports training. In this paper, the semisupervised framework is used to implement motion input and interactive virtual scene algorithms. First, based on Q statistics, virtual simulation and differential selection algorithms are used to select athletic students with strong autonomous learning ability and then use the classifier’s neighbor confidence. The formula selects the student with the highest learning level and marks it. Experimental results show that this method can effectively assist physical education activities and improve students’ learning efficiency. Students’ efficiency in sports has increased by 30%. At the same time, 2/3 of people believe that their interest in sports training has increased 80% and another 90% of college coaches believe that the use of virtual reality technology in physical education is very necessary, which can improve the technical level and training quality of college sports athletes and contribute to the reserve of Chinese competitive sports talents.
The establishment of a simple and effective method for determination of heavy metal ions has great significance due to the high hazard of heavy metal ions to public health and environments. Herein, ...we developed a facile way to synthesise a nanocomposite comprised of carbon nanotubes (CNTs) and metal-organic frameworks (MOFs), and explored its application in constructing an electrochemical (EC) sensor for detection of Cd
2+
in aqueous solutions. The sensing surface was simply fabricated by casting a drop of CNTs-MOFs nanocomposite onto the electrode. After drying at room temperature, the modified electrode was immersed in a Cd
2+
-containing acetate buffer solution to accomplish the EC measurement. The morphology of materials was characterised by SEM. The EC characterisations validated the good conductivity and excellent Cd
2+
-responsive property of CNTs-MOFs. As expected, the analytical performance for Cd
2+
determination was satisfactory with a wide linear detection range of 0.3 μM-150 μM, a limit of detection (LOD) of 0.2 μM and good selectivity. Detection of Cd
2+
in real samples was also realised. Moreover, if under other potentials, even the analysis of other heavy metal ions such as Pb
2+
and Cu
2+
can be achieved with our sensing platform. This work provided a versatile way for detecting heavy metal ions in complicated samples.
Chronic myocardial infarction (MI) results in the formation of arrhythmogenic substrates, causing lethal ventricular arrhythmia (VA). We aimed to determine whether mesenchymal stem cells (MSCs) ...carrying a hepatocyte growth factor (HGF) gene modification (HGF-MSCs) decrease the levels of arrhythmogenic substrates and reduce the susceptibility to developing VA compared with unmodified MSCs and PBS in a swine infarction model.
The left descending anterior artery was balloon-occluded to establish an MI model. Four weeks later, the randomly grouped pigs were administered MSCs, PBS or HGF-MSCs via thoracotomy. After an additional four weeks, dynamic electrocardiography was performed to assess heart rate variability, and programmed electrical stimulation was conducted to evaluate the risk for VA. Then, the pigs were euthanized for morphometric, immunofluorescence and western blot analyses.
The HGF-MSC group displayed the highest vessel density and Cx43 expression levels, and the lowest levels of apoptosis, and tyrosine hydroxylase (TH) and growth associated protein 43 (GAP43) expression. Moreover, the HGF-MSC group exhibited a decrease in the number of sympathetic nerve fibers, substantial decreases in the low frequency and the low-/high- frequency ratio and increases in the root mean square of successive differences (rMSSD) and the percentage of successive normal sinus R-R intervals longer than 50 ms (pNN50), compared with the other two groups. Finally, the HGF-MSC group displayed the lowest susceptibility to developing VA.
HGF-MSCs displayed potent antiarrhythmic effects, reducing the risk for VA.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
CD147, also known as EMMPRIN or basigin, is a transmembrane glycoprotein receptor that activates matrix metalloproteinases and promotes inflammation. CD147 function is regulated by posttranslational ...modifications of which glycosylation has attracted the most attention. In this study, we demonstrated that glycosylated CD147 was the dominant form in heart tissue, and its levels were markedly elevated in response to transverse aortic constriction (TAC). Adeno-associated virus 9-mediated, cardiac-specific overexpression of wild-type CD147 in mice significantly promoted pressure overload-induced pathological cardiac remodeling accompanied by augmented oxidative stress and ferroptosis. By contrast, mutations of CD147 glycosylation sites notably weakened these detrimental effects of CD147. Mechanistically, CD147 exacerbated TAC-induced pathological cardiac remodeling via direct binding with the adaptor molecule TRAF2 and subsequent activation of TAK1 signalling, which was dependent on glycosylation of CD147. Collectively, our findings provide the first evidence that CD147 promoted pathological cardiac remodeling and dysfunction in a glycosylation-dependent manner through binding the adaptor protein TRAF2 and activating the downstream TRAF2-TAK1 signalling pathway. Thus, glycosylation of CD147 may be a potent interventional target for heart failure treatment.
Objective: The objective of the study was to observe and analyze the characteristics of negative emotions and quality of life (QoL) in patients with nonsmall cell lung cancer (NSCLC) from 1 to 3 ...months after surgery, and summarize their population characteristics after surgery. Materials and Methods: The consolidation rehabilitation period was defined as 1 to 3 months after surgery. The main observation indicators were the general Hospital Anxiety and Depression Scale, the European Organization for the Research and Treatment of Cancer Core QoL Questionnaire (EORTC QLQ-C30), and EORTC QLQ-Lung Cancer-13. The secondary observation indicators were peripheral blood cytokines and immune function indicators. Statistical methods such as one-way analysis of variance and logistic regression were used to analyze the characteristics of this part of the population. Results: Among patients who underwent lung surgery, the overall incidence of anxiety during consolidation recovery was 13.66%, and that of depression was 10.38%. The scores of fatigue, pain, dyspnea, insomnia, and loss of appetite were significantly higher than those of other symptoms (P = 0.000). The presence of anxiety was associated with the site of surgery (P < 0.05) and the presence of depression was associated with emotional functioning and dyspnea (P < 0.05). Conclusions: The occurrence of negative emotions in the postoperative NSCLC population is related to gender, and the degree of emotions is negatively correlated with QoL and positively correlated with the degree of symptoms. The main relevant syndromes are the pulmonary and meridian categories.
We aimed to explore the role of NLRP3 inflammasome in Bifidobacterium longum-regulated visceral hypersensitivity of postinfectious irritable bowel syndrome (PI-IBS). Fifty NIH mice were divided into ...five groups (n = 10). The visceral sensitivities of PI-2W and PI-8W groups significantly exceeded than those of normal control groups (P < 0.05), which was significantly decreased in PI-B group (P < 0.05). Significantly more IL-18 and IL-1β were expressed in PI-2W and PI-8W groups than those in normal control groups (P < 0.05), which was significantly decreased in PI-B group (P < 0.05). Bifidobacterium longum may down-regulate IL-18 and IL-1β expressions by inhibiting NLRP3 inflammasome and thus reduce the visceral hypersensitivity of PI-IBS.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
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