In this paper, a broadband circular polarized (CP) reflectarray using a linear polarized (LP) feed is proposed. First, a single‐layer broadband dual‐line polarized reflectarray unit is designed. This ...unit converts the LP incident waves into CP reflected waves by providing a phase difference of 90° in the orthogonal direction components of the incident waves. Based on the units proposed, a 109‐element reflectarray was designed and fabricated. The measured results indicate that the reflectarray has a 1‐dB gain bandwidth of 9.15 to 12.79 GHz, or equivalent to a bandwidth of 33.2%. Its 3‐dB axial ratio (AR) bandwidth for CP operation is 37% (9.06‐13.19 GHz). The reflectarray antenna has a peak gain of 23.53 dBi and its highest aperture efficiency is 64.1% achieved at 10 GHz.
The study was performed to evaluate the effects of the reduced lactate production by sodium oxamate (SO) on growth performance, lactate and glucose and lipid metabolism, and glucose tolerance of
fed ...high-carbohydrate (CHO) diets. In in vitro study, primary hepatocytes were incubated for 48 h in a control medium (5.5 mM glucose), a high-glucose medium (25 mM glucose, HG), or a SO-containing high-glucose medium (25 mM glucose + 50 mM SO, HG-SO). Results indicated lactate and triglyceride (TG) levels, and lactate dehydrogenase a (LDH-a) expression in the HG-SO group were remarkably lower than those of the HG group. In in vivo study,
(5.23 ± 0.03 g) were fed four diets containing a control diet (10% CHO, C) and three SO contents 0 (HC), 100 (HC-SO1), and 200 (HC-SO2) mg·kg
, respectively of high-CHO diets (20% CHO) for 11 wk. High-CHO diets significantly reduced weight gain rate (WGR), specific growth rate (SGR), p-AMPK-to-t-AMPK ratio, and expression of insulin receptor substrate 1 (IRS1), insulin-like growth factor I (IGF-I), insulin-like growth factor I receptor (IGF-IR), fructose-1,6-biphosphatase (FBPase), peroxisome proliferator-activated receptor α (PPARα), and carnitine palmitoyl transferase 1α (CPT1α) compared with the C group, whereas the opposite was true for plasma levels of glucose, TG, lactate, tissue glycogen, and lipid contents, and expression of LDH-a, monocarboxylate transporter 1 and 4 (MCT1 and MCT4), insulin, glucokinase (GK), pyruvate dehydrogenase E1 subunit (PDH), sterol-regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS). The HC-SO2 diets remarkably increased WGR, SGR, p-AMPK-to-t-AMPK ratio, and expression of IRS1, IGF-I, IGF-IR, GK, PDHα, PDHβ, FAS, acetyl-CoA carboxylase 1 (ACC1), PPARα, and CPT1α compared with the HC group. Besides, HC-SO2 diets also enhanced glucose tolerance of fish after a glucose loading. Overall, the reduced lactate production by SO benefits growth performance and glucose homeostasis of high-CHO-fed
through the enhancement of glycolysis, lipogenesis, and fatty acid β-oxidation coupled with the suppression of glycogenesis and gluconeogenesis.
Lead and chromate/dichromate are widely used in manufacturing industry, but Pb(II) and Cr(VI) ions pose fatal threats to human health and the water ecological environment. It is thus highly desirable ...to develop novel and valid strategies for rapid detection and effective decontamination of these heavy metal ions. Design and assembly of luminescent Metal-Organic Framework (LMOFs) for effective recognition and removal of Pb(II) and/or Cr(VI) from water provide a feasible avenue to address this issue. Herein, a novel Cd(II)-MOF, (Cd3L2)·(solvent)x (labeled as compound 1) with bright blue fluorescence was assembled under the solvothermal reaction of triangular ligand 5'-(5-carboxy-1H-benzo dimidazole-2-yl)-1,1':3′,1″-terphenyl-4,4″-dicarboxylic acid (H3L) and Cd(NO3)2. Given that the unique structure features of 3D stacking framework, i. e. strong π···π conjugative effects of organic linkers, plentiful free imidazole N atoms and carboxyl O atoms, Cd-MOF of 1 was deliberately deployed as bifunctional platform to sense Pb(II)/Cr(VI) and photochemically reduce Cr(VI) to Cr(III). Notably, 1 emits durable bright blue fluorescence and performs rapid quenching sense for Pb2+, Cr2O72− and CrO42−, with ultra low detection limits of 1.89, 4.83 and 2.84 ppb, respectively. Moreover, highly toxic Cr(VI) could be rapidly and thoroughly reduced to Cr(III) ions by 1 under the irradiation of mercury lamp, with reliable recycling ability. The possible photoluminescence, quenching and photocatalytic reduction mechanisms also were tentatively proposed.
Highly efficient and bifunctional for cationic Pb(II), anionic Cr(VI) analytes detection and Cr(VI) photochemical reduction decontamination were thoroughly demonstrated by a powerful Cd(II)-Organic Framework platform, performing fairly low detection limits values and rather high/rapid photocatalytic reduction capability. And may open a new avenue for addressing the fatal environmental pollution issues caused by heavy metal ions. Display omitted
•The unique dual-fluorescent probe for cationic Pb(II) and anionic Cr(VI) analytes.•Fairly low detection limits values among all reported MOF-based sensors.•Excellent selectivity, anti-interference abilities and reliable recyclability.•High efficiency, rapid and recyclable photocatalytic reduction abilities for Cr(VI).
A series of novel 6-substituted quinazoline derivatives were synthesised as epidermal growth factor receptor(EGFR) and Human epidermal growth factor receptor 2 (HER2)inhibitors in our lab. The novel ...compounds were measured for their dual enzyme inhibition as well as their cytotoxic activity on MCF7 cell line. The results revealed that all the compounds showed inhibition of both enzymes. Compound 5c showed the best inhibitory activity against both enzymes and IC50 of its was 2.6 nM against EGFR kinases and 4.3 nM against HER2 kinases, respectively. Most of the measured compounds showed to antitumor activity on MCF7.
2 With improvements in medical imaging technology and related basic research, the perception of CSDH as an inflammatory vascular proliferative disease has gradually reached consensus. 3 In addition ...to head trauma, brain atrophy leading to expanded subdural space is the premise and primary reason for the occurrence of CSDH in the older adult population. The study was conducted by the Declaration of Helsinki and was approved by the Institutional Review Board of Capital Medical University. Inclusion criteria were as follows: (1) medical history of head trauma or no clear medical history; (2) symptoms such as headache, vomiting, blurred vision, seizures, memory and mental retardation, or mental disorders; (3) neurologic dysfunction, such as hemiplegia or aphasia; (4) CT showed crescent, half-moon, or fusiform low density appearing mainly below the inner plate cranial bone, possibly accompanied by brain deformation and midline shift; (5) no medication history for CSDH; (6) suspension of anticoagulant drugs such as aspirin for more than 10 days; and (7) non-recurrent CSDH.
Background
The aberrant regulation of cell cycle is significantly correlated with cancer carcinogenesis and progression, in which cell cycle checkpoints control phase transitions, cell cycle entry, ...progression, and exit. However, the integrative role of cell cycle checkpoint-related genes (CRGs) in bladder carcinoma (BC) remains unknown.
Methods
The transcriptomic data and clinical features of BC patients were downloaded from The Cancer Genome Atlas (TCGA), used to identify CRGs correlated with overall survival (OS) by univariate Cox regression analysis. Then, the multivariate and least absolute shrinkage and selection operator (LASSO) Cox regression analyses further developed a prognostic CRG signature, which was validated in three external datasets retrieved from Gene Expression Omnibus (GEO). The receiver operating characteristic curve (ROC) analysis was conducted for evaluating the performance of the CRG signature in prognosis prediction. RNA sequencing (RNA-Seq) was performed to explore the expression difference in the identified CRGs between tumor and normal tissue samples from 11 BC patients in the local cohort. Ultimately, genomic profiles and tumor microenvironment (TME), and the Genomics of Drug Sensitivity in Cancer (GDSC) were investigated to guide precision treatment for BC patients with different CRG features.
Results
The novel constructed 23-CRG prognostic signature could stratify BC patients into high-risk and low-risk groups with significantly different outcomes (median OS: 13.64 vs. 104.65 months). Notably, 19 CRGs were the first to be identified as being associated with BC progression. In three additional validation datasets (GSE13507, GSE31684, and GSE32548), higher CRG scores all indicated inferior survival, demonstrating the robust ability of the CRG signature in prognosis prediction. Moreover, the CRG signature as an independent prognostic factor had a robust and stable risk stratification for BC patients with different histological or clinical features. Then, a CRG signature-based nomogram with a better performance in prognostic prediction concordance index (C-index): 0.76 was established. Functional enrichment analysis revealed that collagen-containing extracellular matrix (ECM), and ECM-related and MAPK signaling pathways were significantly associated with the signature. Further analysis showed that low-risk patients were characterized by particularly distinctive prevalence of
FGFR3
(17.03% vs. 6.67%,
p
< 0.01) and
POLE
alterations (7.97% vs. 2.50%,
p
< 0.05), and enrichment of immune infiltrated cells (including CD8+ T cells, CD4+ naïve T cells, follicular helper T cells, Tregs, and myeloid dendritic cells). RNA-seq data in our local cohort supported the findings in the differentially expressed genes (DEGs) between tumor and normal tissue samples, and the difference in TME between high-risk and low-risk groups. Additionally, CRG signature score plus
FGFR3
status divided BC patients into four molecular subtypes, with distinct prognosis, TME, and transcriptomic profiling of immune checkpoint genes. Of note, CRG signature score plus
FGFR3
status could successfully distinguish BC patients who have a higher possibility of response to immunotherapy or chemotherapy drugs.
Conclusions
The CRG signature is a potent prognostic model for BC patients, and in combination with
FGFR3
alterations, it had more practical capacity in the prediction of chemotherapy and immunotherapy response, helping guide clinical decision-making.
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•A fluorescent probe was synthesized based on benzopyran-chromone for detection of ONOO−.•The probe shows short response time for ONOO− (<30 s).•The probe shows specifically ...selectivity and a very low detection limit of 22.4 nM for ONOO−.•The probe can accurately identify endogenous and exogenous ONOO− in HeLa cells.
Peroxynitrite is widely present in organisms and closely related to many pathophysiological functions. Therefore, it is of great physiological significance to develop capable probes for detecting ONOO−. In this work, a novel fluorescent probe B-Ch was designed based on the intramolecular charge transfer (ICT) effect. By means of molecular engineering, the replacement from diethylamine group to hydroxyl group has improved the detection sensitivity of the probe. After the addition of ONOO−, the solution color and fluorescence showed noticeable changes, which were visible to the naked eye. The probe showed excellent advantages: visualization, good selectivity, low sensitivity (22.4 nM), good stability and biocompatibility, exogenous and endogenous imaging of ONOO− in HeLa cells.
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•A NIR fluorescence probe based on coumarin-cyanine hybrid was prepared for detection of ONOO−.•The probe showed high selectivity and sensitivity for ONOO− with low detection limit ...(41.88 nM).•This probe showed a ratio response to ONOO− with a large Stokes shift (239 nm).•The probe was capable of accurate imaging of ONOO− in mitochondria.
There is an urgent need to develop highly sensitive and selective fluorescence probes for ONOO− in mitochondria. Herein, we reported a ratiometric fluorescent probe COUS with coumarin-cyanine hybrid as fluorophore and C = C bonds as reaction sites of ONOO−. The probe COUS was sensitive and selective to ONOO−, and had a large fluorescence emission shift (239 nm) as well as a low detection limit (41.88 nM). Moreover, COUS showed the mitochondrial targeting ability, and the targeting moiety could dissociate from the probe when reacting with ONOO−, which enabled COUS to accurately detect ONOO− in mitochondria.
The Radio Frequency Interference (RFI) generated by the surrounding environment will significantly reduce the observation efficiency of the large radio telescope. The Tian-Ma Radio Telescope (TMRT) ...has established a RFI monitoring system to keep watch on its surrounding RFI environment chronically in the L, S, C and X bands. The system consists of antennas, receivers, back-ends and control system. To achieve automatic system operation, we design and implement an automation software based on Tango Controls open source framework. This paper will briefly introduce the TMRT RFI Monitoring System (TRMS), and describe the functional design, architecture design and implementation of the automation software. Finally, with the help of this system, we carry out automatic remote monitoring of the RFI environment around the TMRT in the 1.12—12.4 GHz frequency band, and then analyze and verify the observation results.
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