The present work follows a previous report describing the antibacterial activity of silver camphorimine complexes of general formula Ag(NO3)L. The synthesis and demonstration of the antifungal and ...antibacterial activity of three novel Ag(NO3)L complexes (named 1, 2 and 3) is herein demonstrated. This work also shows for the first time that the previously studied complexes (named 4 to 8) also exert antifungal activity. The antibacterial activity of complexes was evaluated against Staphylococcus aureus, Pseudomonas aeruginosa, Burkholderia contaminans and Escherichia coli strains, while antifungal activity was tested against the Candida species C. albicans, C. glabrata, C. parapsilosis and C. tropicalis. The antimicrobial activity of the complexes ranged from very high (complex 4) to moderate (complex 6) or low (complex 8), depending on the structural and electronic characteristics of the camphorimine ligands. Notably, the highest antibacterial and anti-Candida activities do not coincide in the same complex and in some cases they were even opposite, as is the case of complex 4 which exhibits a high anti-bacterial and low antifungal activity. These distinct results suggest that the complexes may have different mechanisms against prokaryotic and eukaryotic cells. The antifungal activity of the Ag(I) camphorimine complexes (in particular of complex 1) was found to be very high (MIC = 2 μg/mL) against C. parapsilosis, being also registered a prominent activity against C. tropicalis and C. glabrata. None of the tested compounds inhibited C. albicans growth, being this attributed to the ability of these yeast cells to mediate the formation of less toxic Ag nanoparticles, as confirmed by Scanning Electron Microscopy images. The high antibacterial and anti-Candida activities of the here studied camphorimine complexes, especially of complexes 1 and 7, suggests a potential therapeutic application for these compounds.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
complex (Bcc) bacteria emerged as opportunistic pathogens in cystic fibrosis and immunocompromised patients. Their eradication is very difficult due to the high level of intrinsic resistance to ...clinically relevant antibiotics. Bcc bacteria have large and complex genomes, composed of two to four replicons, with variable numbers of insertion sequences. The complexity of Bcc genomes confers a high genomic plasticity to these bacteria, allowing their adaptation and survival to diverse habitats, including the human host. In this work, we review results from recent studies using omics approaches to elucidate in vivo adaptive strategies and virulence gene regulation expression of Bcc bacteria when infecting the human host or subject to conditions mimicking the stressful environment of the cystic fibrosis lung.
expressing serotype 3 has a high virulence and a high case fatality ratio. Most studies of serotype 3 pneumococci have focused on a single lineage, the widespread sequence type 180 (ST180). To ...evaluate the serotype 3 lineages causing infections in Mexico, we characterized 196 isolates recovered from 1994 to 2017. The isolates were mostly susceptible to all antimicrobials tested. A single meningitis isolate was resistant to penicillin, and the resistance to erythromycin was 5.2%. The isolates represented the widely disseminated clonal complex 180 (CC180;
= 140), the unusual CC4909 (
= 42), CC260 (
= 11), and a few singletons (
= 3). CC260 was less frequent among pneumococcal invasive disease isolates than CC180 and CC4909 (
= 0.015). There was a decrease of CC4909 (
< 0.001) following PCV13 introduction (2012 to 2017). The CC4909 isolates were represented mostly by ST1119 (
= 40), seemingly having a restricted geographic origin, with isolates in the PubMLST database having been recovered only in Mexico, the United States, and Germany. A genomic analysis of publicly available genomes showed that ST1119 isolates have less than 32% similarity with ST180 isolates, indicating that these lineages are more separated than revealed by traditional multilocus sequence typing. Considering the suggestions of a lower efficacy of the 13-valent pneumococcal conjugate vaccine against serotype 3, the different dynamics of the two major serotype 3 lineages in Mexico following the introduction of PCV13 should be closely monitored.
Five new silver camphorimine complexes of general formula Ag(NO3)(Y)L were synthesized and fully characterized using spectroscopic and analytical techniques. The structure of ...Ag(NO3)(OC10H14NC6H4NC10H14O) () was analyzed using single crystal X-ray diffraction, showing that it arranges as a coordination polymer formed by sequential Ag(NO3) units bridged by the bi-camphor ligand (). The antimicrobial properties of the new complexes were screened using the disk diffusion method and their Minimal Inhibitory Concentrations (MIC) were assessed against selected bacterial strains of the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli, Pseudomonas aeruginosa, and Burkholderia contaminans. The lowest MICs were observed for , with estimated values of 72, 20, 32 and 19 μg mL(-1) for S. aureus, E. coli, B. contaminans, and P. aeruginosa, respectively. In the case of S. aureus, similar MIC values were obtained for silver nitrate and compound . All five compounds were bactericidal when used in concentrations equal or above the MIC value, as found by enumerating the total colony forming units (CFUs) after incubation in their presence.
Hfq has emerged in recent years as a master regulator of gene expression in bacteria, mainly due to its ability to mediate the interaction of small noncoding RNAs with their mRNA targets, including ...those related to virulence in Gram-negative bacteria. In this work, we review current knowledge on the involvement of Hfq in the regulation of virulence traits related to secretion systems, alternative sigma factors, outer membrane proteins, polysaccharides and iron metabolism. Recent data from transcriptomics and proteomics studies performed for major pathogens are included. We also summarize and correlate current knowledge on how Hfq protein impacts pathogenicity of bacterial pathogens.
Five new silver camphorimine complexes of general formula Ag(NO
3
)
Y
L were synthesized and fully characterized using spectroscopic and analytical techniques. The structure of Ag(NO
3
)(OC
10
H
14
...NC
6
H
4
NC
10
H
14
O) (
III
) was analyzed using single crystal X-ray diffraction, showing that it arranges as a coordination polymer formed by sequential Ag(NO
3
) units bridged by the bi-camphor ligand (
2A
L
). The antimicrobial properties of the new complexes were screened using the disk diffusion method and their Minimal Inhibitory Concentrations (MIC) were assessed against selected bacterial strains of the Gram-positive
Staphylococcus aureus
and the Gram-negative
Escherichia coli
,
Pseudomonas aeruginosa
, and
Burkholderia contaminans
. The lowest MICs were observed for
III
, with estimated values of 72, 20, 32 and 19 μg mL
−1
for
S. aureus
,
E. coli
,
B. contaminans
, and
P. aeruginosa
, respectively. In the case of
S. aureus
, similar MIC values were obtained for silver nitrate and compound
III
. All five compounds were bactericidal when used in concentrations equal or above the MIC value, as found by enumerating the total colony forming units (CFUs) after incubation in their presence.
Ag(NO
3
)(OC
10
H
14
NC
6
H
4
NC
10
H
14
O) is the first bi-camphor silver coordination polymer structurally characterized. It displays bactericidal activity against Gram-negative and Gram-positive strains comparable to drugs in use.
to assess the relationship between health-related quality of life with depression and self-esteem of people after kidney transplantation.
a cross-sectional study of 47 outpatients from October 2016 ...to February 2017. The following tools were applied: The Medical Outcomes Study 36-Item Short-Form Health Survey, Beck Depression Inventory and Rosenberg Self-Esteem Scale. Descriptive statistics and Spearman correlation were used.
women had lower scores for health-related quality of life. Young adults, people with up to one and a half years of transplantation and those who had dialysis for more than one year had higher scores.
the health-related quality of life of people with chronic kidney disease after transplantation ranged from good to excellent. The presence of depression was not identified. The relationship of data indicates that the higher the quality of life, the better the self-esteem assessment.
Introduction: Europe presents 19% of the population aged 65 or over (elderly), who are especially susceptible to inadequate prescriptions (potentially
inappropriate medications (PIM) and potential ...prescribing omissions (PPO)), identified by the STOPP and START criteria. This study has as main objectives
the identification of PIM and PPO and associated factors. Material and Methods: This is a cross sectional study, using a sample of 254 elderly individuals
from the Internal Medicine Service. Results: 81.9% of patients are polymedicated, 72.4% have at least one PIM and 57.5% have at least one PPO. The
most frequently identified PIM was benzodiazepine and PPO was the anti-pneumococcal vaccine. There was a direct correlation between the number
of PIM and chronic medication (r(254)=0.348, p<0.001) and inverse with the Katz scale (dependence) (r(254)=-0.324, p<0.001). In the cases of the
domicile it was verified association between PIM and the days of internment (U=3653, p=0.025). PIM were associated with death in less than 6 months
after discharge (U=3396, p=0.007) and the presence of intercurrences at admission (U=5766, p=0.005). There is a relationship between the number of
co-morbidities and having at least one PIM (U=5378, p=0.041) or at least one PPO (U=6271, p=0.005). Diabetes mellitus (DM) type 2, neurological and
psychiatric disease are associated with PIM, while obesity, DM type 2, arterial hypertension, dyslipidemia and cardiac pathology with PPO. Discussion and
Conclusion: In a population that is older each year, with more comorbidities and more polymedicated, PIM and PPO are increasingly relevant.
K
Introduction: The European population is aging, for every 100 young people there are 128 elderly. There is a decrease in the number of elderly people
living in their family environment. The main ...objective of this study is to identify the differences in the general health status of the elderly according to their
residence: in families or in others types of centres (nursing home, host family or national integrated network of integrated care (NINIC)).
Material and Methods: This is an observational study, using a sample of 254 elderly patients admitted to the Internal Medicine Service.
Results: Of the patients studied, 78,7% lived at home/with relatives, 15,4% in nursing homes, 3,15% in host families and 2,75% in NINIC. Age ranged
from 65 to 99 years, with a median of 80 years (SD=7,97). Patients from their homes were more independent/mild dependence (X(3)=59.8, p<0.001),
although there were no significant differences in the number of comorbidities (t(252)=0.029, p=0.999). However, patients from others centres had more
neurological pathology (X(1)=12.39, p<0.001). There were no statistically significant differences in the number of medications usually taken, although
patients from others centres had more potentially inappropriate medications (t(252)=5.85, p<0.001) and potential prescribing omissions (t(252)=3.71,
p<0.001). Patients at home had fewer rehosts (X(1)=5.14, p=0.039). There were more intercurrences (X(1)=19.0, p<0.001) and more deaths at 6
months after discharge (X(1)=16.3, p<0.001) from others centres.
Conclusion: In a population less attended by families, these differences in relation to their dwelling places are paramount
ABSTRACT Objective: To understand how self-care is carried out by people with chronic kidney disease on hemodialysis. Method: A qualitative approach was used. Data was collected through ...semi-structured interviews in 12 individuals on hemodialysis in Chile between September and December 2018. Dorothea Orem's self-care theory was used for directed content analysis. Results: Three categories emerged: self-care requirements, self-care deficit, and education and information management for self-care. People were aware of the importance of carrying out their self-care. They also stated not to carry out the care actions rigorously enough showing some limitations. Finally, people’s knowledge about their condition was usually acquired from the Internet and from their own experience rather than through consultations with a health team. Considerations: People are conscious about their role in their health in a flexible way. The health team should know how to recognize conditions interfering in people’s self-care and help to overcome them.
RESUMO Objetivo: Compreender como o autocuidado é realizado por pessoas com doença renal crônica em hemodiálise. Método: Abordagem qualitativa com entrevistas semiestruturadas com 12 pessoas que realizavam hemodiálise no Chile entre os meses de setembro e dezembro de 2018. A análise de conteúdo foi conduzida pela teoria do autocuidado de Dorothea Orem. Resultados: Surgiram três categorias: requisitos de autocuidado, déficit de autocuidado e educação e manejo de informação para o autocuidado. As pessoas reconheceram a importância de realizar seu autocuidado. No entanto, referiram não realizar o cuidado com o rigor que deveriam. Os conhecimentos que possuíam foram adquiridos na internet ou de sua própria experiência, sem recorrer à equipe de saúde. Conclusões: As pessoas são conscientes de seu papel na manutenção de sua saúde, mas de maneira flexível. A equipe de saúde deve saber reconhecer os aspectos que interferem no autocuidado e ajudar as pessoas a superá-los.
RESUMEN Objetivo: Comprender cómo se realiza el autocuidado en personas con enfermedad renal crónica en tratamiento de hemodiálisis. Método: Abordaje cualitativo con entrevistas semi-estructuradas con 12 personas en tratamiento de hemodiálisis en Chile entre los meses de septiembre y diciembre de 2018. Se empleó el análisis de contenido dirigido por la teoría de autocuidado de Dorothea Orem. Resultados: Surgieron tres categorías: requisitos de autocuidado, déficit de autocuidado, y educación y manejo de información para el autocuidado. Las personas conocían la importancia de ejecutar su autocuidado. Sin embargo, no realizaban los cuidados con rigurosidad. Adquirían los conocimientos por Internet y a partir de la propia experiencia, no recurriendo al equipo de salud ante dudas. Conclusiones: Las personas demostraron ser conscientes de su rol en preservar su salud, pero de forma flexible. El equipo de salud debe reconocer los aspectos que interfieren en el autocuidado y ayudar a las personas a superarlos.
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