This article describes early results of a new instrument for measuring quality of life--the French Subjective Quality of Life Profile (SQLP) questionnaire. This 36-item, self-administered ...questionnaire has been previously validated in a large sample population with somatic disorders. It is characterized by its multidimensional pattern and subjective approach (i.e., the degree of satisfaction with various domains of life, the degree of change anticipated and the importance attributed to these domains). The SQLP was tested with three psychiatric patient samples: people with depression, psychosis or substance abuse. Preliminary findings indicate that the questionnaire is useful in describing psychiatric patients, their characteristics, and explaining some of their changes.
This study describes an inquiry concerning patients (65 in all) who were referred to the Socio-Professional Rehabilitation Department (CHS Le Vinatier, Bron) over 14 months. The aim was to study the ...influence of occupational therapy on the clinical evolution of psychotic patients, and more specifically on their quality of life. Two evaluations using questionnaires filled out both by the patients and the medical staff were performed. One evaluation was carried out at admission, and the second 3 months later. The studied variables were the patients' symptoms, quality of life and ability to perform a task. Validation studies showed the answers to questionnaires to be reliable indicators. It was found that this group of psychotic patients was anxious rather than depressed, and fairly autonomous; their dissatisfaction mainly concerned material conditions. The initial factors most related to quality of life after 3 months of treatment were mainly connected with their ability to perform a task, and to a lesser extent with the initial gravity of symptoms.
A range of "bioartificial" collagen/poly(vinyl alcohol) blends have been produced, cast as films and cross-linked using either glutaraldehyde or a dehydrothermal treatment (DRT). Films were used as ...substrates for the culture of osteoblast-like cells. The attachment, adhesion and proliferative responses of these cells to the range of films were examined using proliferation assays, light, electron and confocal microscopy. There was an inverse relationship between collagen content of glutaraldehyde cross-linked films and the extent of cell proliferation on them. A cytotoxicity assay demonstrated no toxic effect related to increasing collagen content. The greatest differences in cell responses observed were associated with the choice of cross-linking method. Films cross-linked with glutaraldehyde showed variation related to collagen content in cell adhesion, proliferation and morphology. Such differences were not apparent with the DHT cross-linked films. Collagen/PVA "bioartificial" films can be dehydrothermally cross-linked to increase biological stability and reduce water solubility. The method of cross-linking employed is the greater influence in determining osteoblast compatibility with these materials. The DRT cross-linking method is a preferable alternative to the use of glutaraldehyde. Collagen /PVA bioartificial films cross-linked by the DHT method have shown potential for biocompatibility with osteoblasts.
1.
1. The behavioral and anticonvulsant effects of several 1,4-benzodiazepine (BDZ) and azirino1,2-
d 1, 4benzodiazepine (ABDZ) derivatives were studied after intraperitoneal administration in DBA/2 ...mice, a strain genetically susceptible to sound-induced seizures.
2.
2. The anticonvulsant effects were evaluated on seizures evoked by means of auditory stimulation (109 dB, 12–16 kHz) in animals placed singly under a Perspex dome.
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3. The 1,4-benzodiazepines were generally more potent than the related azirino1,2-d1,4benzodiazepine derivatives which, however, showed a remarkable anticonvulsant activity. The rank order of potency for anticonvulsant activity was flunitrazepam > diazepam > pinazepam > ABDZ5 > ABDZ4 > prazepam > halazepam > ABDZ1 > ABDZ3 > camazepam > ABDZ6 > ABDZ2.
4.
4. The impairment of locomotor performance following intraperitoneal (IP) administration of the aforementioned derivatives was also evaluated by means of rotarod test. The rank order of potency for impairment of coordinated motor movements was pinazepam > flunitrazepam > diazepam > ABDZ5 > prazepam > halazepam > ABDZ4 > ABDZ3 > ABDZ1 > camazepam > ABDZ2 = ABDZ6.
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5. A hypothermic activity was observed after the highest doses of the benzodiazepines studied.
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6. The potency of various 1,4-benzodiazepines and azirino1,2-d1,4benzodiazepines as inhibitors of specific
3Hflumazenil binding to membranes from cerebellum or cortex was evaluated. In general, they inhibited
3Hflumazenil binding at the micromolar range. However, some ABDZ derivatives, although active as anticonvulsants, failed to displace
3Hflumazenil.
7.
7. The azirino1,2-
d 1,4benzodiazepine derivatives are more lipophilic than the related benzodiazepines, but the different degree of anticonvulsant activity and impairment of coordinated motor movements cannot be directly related to the lipophilicity of the compounds studied.
8.
8. The pharmacologic actions of ABDZ4 and ABDZ5, which appeared as the most potent anticonvulsants of the azirino1,2-
d 1,4benzodiazepine derivatives, were significantly reduced by treatment with flumazenil (8.24μmol/kg IP) suggesting a clear involvement of benzodiazepine mechanisms in the anticonvulsant activity of these compounds or their metabolites.
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9. The anticonvulsant activity of ABDZ4 and ABDZ5 was also evaluated against seizures induced by the two β-carbolines, methylβ-carboline-3-carboxylate (β-CCM) and methyl6,7-dimethoxy-4-ethyl-β-carboline-3-carboxylate (DMCM), in DBA/2 mice. Both ABDZ4 and ABDZ5 gave better protection against the seizures induced by β-CCM than DMCM, suggesting a preferential action on BDZ1 receptors.
Status of the LVD experiment at Gran Sasso Laboratory Pless, I.A.; Deutsch, M.; Guo, Y. ...
AIP Conference Proceedings (American Institute of Physics); (United States),
02/1992, Letnik:
272:2
Conference Proceeding
Recenzirano
The LVD particle astrophysics detector at Gran Sasso Laboratory is described and the status of the detector is presented.
Regulatory cell therapies have shown promise in tolerance-induction protocols in living donor organ transplantation. These protocols should be pursued in deceased donor transplantation. Donor ...peripheral mononuclear cells (PBMCs) are an optimal source of donor antigens for the induction of donor-specific regulatory cells. During the development of a regulatory cell tolerance-induction protocol with organs from deceased donors, we compared 3 methods of obtaining PBMCs from deceased donors focusing on cell yield, viability, and contamination of unwanted cell types. PBMC procurement methods: 1. During organ procurement at the time of cold perfusion, blood was collected from the vena cava and placed into a 10-liter blood collection bag, and thereafter transported to Karolinska University Hospital, where leukapheresis was performed (BCL). 2. Blood was collected via the vena cava into blood donation bags before cold perfusion. The bags underwent buffy coat separation and thereafter automated leukocyte isolation system (BCS). 3. To collect PBMCs, leukapheresis was performed via a central dialysis catheter on deceased donors in the intensive care unit (ICU) prior to the organ procurement procedure (LEU).All 3 methods to obtain PBMC from deceased donors were safe and did not affect the procurement of organs. BCL contained around 50% of NK cells in lymphocytes population. LEU had a highest yield of donor PBMC among 3 groups. LEU had the lower amount of granulocyte contamination, compared to BCS and BCL. Based on these results, we choose LEU as the preferred method to obtain donor PBMC in the development of our tolerance-induction protocol.
Anthocyanin-rich extracts, potent antioxidants and commercially available food coloring agents, have been reported to inhibit growth of various cancer cell lines. We investigated the effect of ...semipurified anthocyanin-rich extract from fruits of Aronia meloncarpa, on normal colon and colon cancer cell lines. A 24-h exposure to 50 micrograms monomeric anthocyanin/ml of Aronia extract resulted in 60% growth inhibition of human HT-29 colon cancer cells. The treated cells showed a blockage at G1/G0 and G2/M phases of the cell cycle. The cell cycle arrest coincided with an increased expression of the p21WAF1 and p27KIP1 genes and decreased expression of cyclin A and B genes. Prolonged exposure to the extract resulted in no further change in the cell number, indicating a cytostatic inhibition of cell growth. NCM460 normal colon cells demonstrated <10% growth inhibition at the highest concentration of 50 micrograms/ml extract. A 35% decrease in the cyclooxygenase-2 gene expression was observed within 24 h of exposure of HT-29 cells but did not translate into decreased protein levels or protein activity. These results support the need for further research to identify the specific component(s) in this extract that suppress cancer cell growth and the genes affected by these natural compounds.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, OILJ, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK, VSZLJ
Pigments and phenolics that absorb ultraviolet light (UV) are involved in the protection of the photosynthetic apparatus during periods of high ultraviolet‐B (UV‐B) radiation and can be of benefit to ...turfgrasses. This study initiated in October 2010 and repeated in March 2011 aimed to characterize protective pigment responses to elevated UV‐B in cool‐season turfgrass. Tall fescue (Festuca arundinacea Schreb.), perennial ryegrass (Lolium perenne L.), and creeping bentgrass (Agrostis stolonifera L.) cultivars L93 and Penncross were tested. Turfgrass pigment responses were measured over a 1‐wk period during which they were subjected to 16 kJ m−2 d−1 of UV‐B in growth chambers. Photoperiod was 14 h and plants were subjected to 26.2 mol m−2 d−1 photosynthetically active radiation (PAR) at 20° C day and 17°C night. Turfgrass samples were collected at Day 0, 1, 3, 5, and 7. Measurements included chlorophyll fluorescence, chlorophyll pigmentation, and flavonoid, phenolic, anthocyanins, and carotenoid concentrations. Chlorophyll fluorescence increased and chlorophyll quantities decreased significantly (P < 0.05) in UV‐B conditions compared to control. All species had significantly (P < 0.05) higher quantities of total phenolics and flavonoids at the top of the tissue canopy relative to roots and shoot tissue near the soil surface. Anthocyanins were only found in creeping bentgrass L93. Carotenoids, zeaxanthin, and β‐carotene declined in the UV‐B treatment for both creeping bentgrass L‐93 and Penncross after 7 d, but did not decrease for perennial ryegrass or tall fescue. Carotenoids may play a greater role in UV‐B tolerance than anthocyanins in cool‐season turfgrasses due to their ubiquitous presence.
Although oral motor therapy is sometimes used to treat articulation disorders in school-age children, several reports question its efficacy. In this case study, four first-grade students, two boys ...and two girls, received 15 half-hour sessions of oral motor treatment based on Easy Does it for ArticulationAn Oral Motor Approach (). Pre- and post-test measures of the childrenʼs articulation indicated no real differences in speech production. These results question the efficacy of general and discrete oral motor exercises because they did not enhance the childrenʼs speech production.
![Azirino[1, 2- d][1, 4]benzo...](http://api.summon.serialssolutions.com/2.0.0/image/issn/XR4PS5YY4K/0306-3623/small "Azirino[1, 2- d][1, 4]benzodiazepine derivatives and related 1,4-benzodiazepines as anticonvulsant agents in DBA/2 mice")
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