The quasifree
photon beam asymmetry,
, has been measured at photon energies,
, from 390 to 610 MeV, corresponding to center of mass energy from 1.271 to 1.424 GeV, for the first time. The data were ...collected in the A2 hall of the MAMI electron beam facility with the Crystal Ball and TAPS calorimeters covering pion center-of-mass angles from 49
to 148
. In this kinematic region, polarization observables are sensitive to contributions from the
and
(1440) resonances. The extracted values of
have been compared to predictions based on partial-wave analyses (PWAs) of the existing pion photoproduction database. Our comparison includes the SAID, MAID and Bonn-Gatchina analyses; while a revised SAID fit, including the new
measurements, has also been performed. In addition, isospin symmetry is examined as a way to predict
photoproduction observables, based on fits to published data in the channels
,
and
.
The quasifree
γ
→
d
→
π
0
n
(
p
)
photon beam asymmetry,
Σ
, has been measured at photon energies,
E
γ
, from 390 to 610 MeV, corresponding to center of mass energy from 1.271 to 1.424 GeV, for the ...first time. The data were collected in the A2 hall of the MAMI electron beam facility with the Crystal Ball and TAPS calorimeters covering pion center-of-mass angles from 49
∘
to 148
∘
. In this kinematic region, polarization observables are sensitive to contributions from the
Δ
(
1232
)
and
N
(1440) resonances. The extracted values of
Σ
have been compared to predictions based on partial-wave analyses (PWAs) of the existing pion photoproduction database. Our comparison includes the SAID, MAID and Bonn–Gatchina analyses; while a revised SAID fit, including the new
Σ
measurements, has also been performed. In addition, isospin symmetry is examined as a way to predict
π
0
n
photoproduction observables, based on fits to published data in the channels
π
0
p
,
π
+
n
and
π
-
p
.
The quasifree
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
...\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\overrightarrow{\gamma } d\rightarrow \pi ^0n(p)$$\end{document}
γ
→
d
→
π
0
n
(
p
)
photon beam asymmetry,
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\varSigma $$\end{document}
Σ
, has been measured at photon energies,
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$E_\gamma $$\end{document}
E
γ
, from 390 to 610 MeV, corresponding to center of mass energy from 1.271 to 1.424 GeV, for the first time. The data were collected in the A2 hall of the MAMI electron beam facility with the Crystal Ball and TAPS calorimeters covering pion center-of-mass angles from 49
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$^\circ $$\end{document}
∘
to 148
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$^\circ $$\end{document}
∘
. In this kinematic region, polarization observables are sensitive to contributions from the
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\varDelta (1232)$$\end{document}
Δ
(
1232
)
and
N
(1440) resonances. The extracted values of
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\varSigma $$\end{document}
Σ
have been compared to predictions based on partial-wave analyses (PWAs) of the existing pion photoproduction database. Our comparison includes the SAID, MAID and Bonn–Gatchina analyses; while a revised SAID fit, including the new
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\varSigma $$\end{document}
Σ
measurements, has also been performed. In addition, isospin symmetry is examined as a way to predict
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\pi ^0n$$\end{document}
π
0
n
photoproduction observables, based on fits to published data in the channels
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\pi ^0p$$\end{document}
π
0
p
,
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\pi ^+n$$\end{document}
π
+
n
and
\documentclass12pt{minimal}
\usepackage{amsmath}
\usepackage{wasysym}
\usepackage{amsfonts}
\usepackage{amssymb}
\usepackage{amsbsy}
\usepackage{mathrsfs}
\usepackage{upgreek}
\setlength{\oddsidemargin}{-69pt}
\begin{document}$$\pi ^-p$$\end{document}
π
-
p
.
The quasifree $\overrightarrow{\gamma} d\to\pi^0n(p)$ photon beam asymmetry,
$\Sigma$, has been measured at photon energies, $E_\gamma$, from 390 to 610
MeV, corresponding to center of mass energy ...from 1.271 to 1.424 GeV, for the
first time. The data were collected in the A2 hall of the MAMI electron beam
facility with the Crystal Ball and TAPS calorimeters covering pion
center-of-mass angles from 49 to 148$^\circ$. In this kinematic region,
polarization observables are sensitive to contributions from the $\Delta
(1232)$ and $N(1440)$ resonances. The extracted values of $\Sigma$ have been
compared to predictions based on partial-wave analyses (PWAs) of the existing
pion photoproduction database. Our comparison includes the SAID, MAID, and
Bonn-Gatchina analyses; while a revised SAID fit, including the new $\Sigma$
measurements, has also been performed. In addition, isospin symmetry is
examined as a way to predict $\pi^0n$ photoproduction observables, based on
fits to published data in the channels $\pi^0p$, $\pi^+n$, and $\pi^-p$.
The quasifree \(\overrightarrow{\gamma} d\to\pi^0n(p)\) photon beam asymmetry, \(\Sigma\), has been measured at photon energies, \(E_\gamma\), from 390 to 610 MeV, corresponding to center of mass ...energy from 1.271 to 1.424 GeV, for the first time. The data were collected in the A2 hall of the MAMI electron beam facility with the Crystal Ball and TAPS calorimeters covering pion center-of-mass angles from 49 to 148\(^\circ\). In this kinematic region, polarization observables are sensitive to contributions from the \(\Delta (1232)\) and \(N(1440)\) resonances. The extracted values of \(\Sigma\) have been compared to predictions based on partial-wave analyses (PWAs) of the existing pion photoproduction database. Our comparison includes the SAID, MAID, and Bonn-Gatchina analyses; while a revised SAID fit, including the new \(\Sigma\) measurements, has also been performed. In addition, isospin symmetry is examined as a way to predict \(\pi^0n\) photoproduction observables, based on fits to published data in the channels \(\pi^0p\), \(\pi^+n\), and \(\pi^-p\).
Mesenchymal stromal cells (MSCs) are trophic immunomodulatory cells residing in tissue stroma. MSCs are deployed as cellular therapy to treat a multitude of diseases. Research to date supports that ...MSCs possess niche cell functionalities that promote local epithelial stem and progenitor cell regeneration with their trophic secretome comprising factors such as R-spondin and Wnt. Bone marrow and adipose are common sources of MSCs with unique attributes in niche functionality. We recently described minor salivary glands as an easily accessible source of pharmaceutical MSCs. It is unclear how the trophic secretome of various sources of MSC compare. The objective of this study was to define the trophic secretome of MSCs derived from marrow (M), adipose tissue (AT), and minor salivary glands (SG) alone and with cytokine stimulation.
MSCs were derived from human bone marrow MSC(M) (n=3), omental adipose tissue MSC(AT) (n=3), and labial salivary glands MSC(SG) (n=9). MSCs were isolated, expanded, and frozen from each tissue. Thawed MSCs from each tissue source were cultured to 80% confluence in standard culture media with platelet lysate supplement for all MSCs. MSCs were treated with IFNγ (10ng/mL), TNFα (10ng/mL), or TGFβ (2.5ng/mL). After 48 hrs of culture, the conditioned media was used for ELISA of epithelial morphogens Wnt2b and R-spondin3 (RSPO3).
The production of R-spondin was similar between human MSCs, regardless of tissue source (Fig 1a). We found that human MSC(M) expressed more Wnt2B than MSC(SG) or MSC(AT) (Fig 1b). RSPO3 production is synergistically increased by treatment with both IFNγ and TNFα in all MSCs, regardless of source (Fig 2a). Treatment of MSCs with IFNγ/TNFα reduced differences in Wnt2b production by MSC source (Fig 2b). In contrast, treatment with IFNγ and TGFβ led to lower RSPO3 production (Fig 2c). Under these same cytokine treatment conditions (IFNγ/TGFβ), Wnt2b production remained higher from MSC(M) than MSC(SG) or MSC(AT)s (Fig 2d).
MSCs derived from certain tissues may have differing trophic effects. Our findings support that tissue source is a factor affecting the MSC secretome in humans. Furthermore, cytokine stimulation can enhance or mitigate the trophic secretome differentially. RSPO3 secretion, responsible for Lgr4-driven progenitor epithelial cell stimulation, is optimized under dual stimulatory conditions with both IFNγ and TNFα.
PDF
